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The map label for this gene is tuf1 [H]

Identifier: 154245621

GI number: 154245621

Start: 1895588

End: 1896778

Strand: Direct

Name: tuf1 [H]

Synonym: Xaut_1677

Alternate gene names: 154245621

Gene position: 1895588-1896778 (Clockwise)

Preceding gene: 154245620

Following gene: 154245622

Centisome position: 35.71

GC content: 65.74

Gene sequence:

>1191_bases
ATGGCCAAAGAGAAGTTCAACCGCTCGAAGCCGCACTGCAACATCGGCACGATCGGTCACGTGGATCATGGCAAGACGTC
GCTGACTGCGGCGATCACGAAGATCCTTGCGGAGACGGGCGGCGCGACGTTCACGGCGTACGACCAGATCGACAAGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATCTCGACCGCTCACGTGGAATACGAGACCCAGAACCGCCACTACGCGCACGTG
GACTGCCCCGGGCACGCCGACTATGTGAAGAACATGATCACCGGCGCGGCGCAGATGGACGGCGCGATCCTGGTGGTGTC
GGCGGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGTCAGGTCGGCGTGCCGGCGCTGGTGGTGT
TCCTCAACAAGTGCGACATGGTCGACGATGAGGAGCTGCTGGAGCTGGTCGAGCTGGAAGTGCGCGAGCTGCTGTCCAAG
TACGACTTCCCCGGCGACGACATCCCGATCATCCGTGGCTCGGCGCTGGTGGCGCTGGAGAACGGCGATCCGAAGCTCGG
CCGCGACGCGGTGCTGAAGCTGATGGAGGCGGTCGACGCCTACATCCCGCAGCCCGAGCGTCCGGTGGACCTGCCGTTCC
TGATGCCCATCGAGGACGTGTTCTCGATCTCGGGCCGCGGCACGGTGGTGACCGGCCGCGTGGAGCGCGGCATCATCAAG
GTCGGTGAGGAAGTGGAAATCGTGGGCATCCGCCCGACGCAGAAGACCACGGTCACCGGCGTCGAGATGTTCCGTAAGCT
GCTCGACCAGGGCCAGGCCGGTGACAACGTCGGCATCCTGGTGCGCGGCACCAAGCGTGAGGACGTGGAGCGCGGCCAGG
TGGTGTGCAAGCCGGGTACGGTGAAGCCACACACCAAGTTCAAGGCCGAAGCCTACATCCTGACCAAGGAAGAGGGCGGC
CGTCACACCCCGTTCTTCACCAACTACCGCCCGCAGTTCTACTTCCGGACCACGGACGTGACCGGCGTGTGCACGCTGCC
GGAAGGCACCGAGATGGTGATGCCGGGCGACAACGTGTCCATGGACGTGGCGCTGATCGTTCCGATCGCCATGGAAGAGA
AGCTGCGCTTCGCCATCCGCGAGGGTGGCCGCACGGTGGGCGCCGGCGTCGTCGCCTCCATCATCGAGTGA

Upstream 100 bases:

>100_bases
TCGCGCAGGAGGTCCAGGCCAAGTACGCCTGATGGCGTCGGTCTGAGCCTTCACGAGTTTCACCCTTCAGGTTCGCCAGA
GAGACCCAACGGAGAGTCCA

Downstream 100 bases:

>100_bases
TCTGACAAGAAAAACGGGGACGCGCCGCGGAACGCGCGCGTCCTTCATTCCCCGGAGCCTTTGAGCCATGAACGGCCAGA
ACATTCGAATTCGCCTGAAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFNRSKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLSK
YDFPGDDIPIIRGSALVALENGDPKLGRDAVLKLMEAVDAYIPQPERPVDLPFLMPIEDVFSISGRGTVVTGRVERGIIK
VGEEVEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNVGILVRGTKREDVERGQVVCKPGTVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVSMDVALIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKEKFNRSKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLSK
YDFPGDDIPIIRGSALVALENGDPKLGRDAVLKLMEAVDAYIPQPERPVDLPFLMPIEDVFSISGRGTVVTGRVERGIIK
VGEEVEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNVGILVRGTKREDVERGQVVCKPGTVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVSMDVALIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKEKFNRSKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLSKY
DFPGDDIPIIRGSALVALENGDPKLGRDAVLKLMEAVDAYIPQPERPVDLPFLMPIEDVFSISGRGTVVTGRVERGIIKV
GEEVEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNVGILVRGTKREDVERGQVVCKPGTVKPHTKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVSMDVALIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.2752293577982, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.2752293577982, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=30.7174887892377, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=29.9776286353468, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI194018522, Length=320, Percent_Identity=29.6875, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018520, Length=320, Percent_Identity=29.6875, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=320, Percent_Identity=29.6875, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=29.9382716049383, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI54607086, Length=320, Percent_Identity=29.375, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=26.4808362369338, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.4683544303797, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.4683544303797, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=383, Percent_Identity=29.5039164490862, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=31.8840579710145, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789108, Length=320, Percent_Identity=28.125, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.5, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=29.6803652968037, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=28.8636363636364, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=28.6363636363636, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=30.1003344481605, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=27.7620396600567, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.3504273504274, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=26.4285714285714, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=67.6767676767677, Blast_Score=544, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=31.7460317460317, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=31.7460317460317, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.9259259259259, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.6135693215339, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=285, Percent_Identity=27.3684210526316, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=30.4093567251462, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=26.6666666666667, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.5063613231552, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.5063613231552, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.9210526315789, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.9210526315789, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.9210526315789, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.9210526315789, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=30.1969365426696, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=30.1969365426696, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.023102310231, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=30.7246376811594, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.080118694362, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=26.4285714285714, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=26.4285714285714, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43263; Mature: 43132

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFNRSKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLSKYDFPGDDIPIIRGSALVALE
HHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEE
NGDPKLGRDAVLKLMEAVDAYIPQPERPVDLPFLMPIEDVFSISGRGTVVTGRVERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNVGILVRGTKREDVERGQVVCKPGT
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVS
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MDVALIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFNRSKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLSKYDFPGDDIPIIRGSALVALE
HHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEE
NGDPKLGRDAVLKLMEAVDAYIPQPERPVDLPFLMPIEDVFSISGRGTVVTGRVERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNVGILVRGTKREDVERGQVVCKPGT
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVS
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MDVALIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA