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The map label for this gene is mdh2 [H]

Identifier: 154245617

GI number: 154245617

Start: 1890951

End: 1891934

Strand: Direct

Name: mdh2 [H]

Synonym: Xaut_1673

Alternate gene names: 154245617

Gene position: 1890951-1891934 (Clockwise)

Preceding gene: 154245616

Following gene: 154245618

Centisome position: 35.62

GC content: 66.97

Gene sequence:

>984_bases
ATGACCAAACCCGCCGTTCGCGTCGCTGTCACGGGCGCCGCCGGTCAGATTTGCTATTCGCTGCTTTTCCGCATCGCCAA
CGGCGACATGTACGGCAAGGACCAGCCGGTCATCCTCCAGCTTCTGGACCTGCCGCAGGCGCAGGCCGCCGTCGGCGGCG
TCGTGATGGAGCTCGAGGACTGCGCGTTCCCGAACCTTGCCGGCGTGGTCATCACCGACGATCCCAAGGTGGCGTTCAAG
GACATCGACGCTGCGGTGCTCGTCGGCGCCCGTCCGCGCTCTAAGGGCATGGAACGCGCCGACCTGCTGTCCGCCAACGC
CGAGATCTTCAAGGTCCAGGGCAAGGCGCTGAACGAGGTGGCCAAGCGCGACGTGAAGGTGCTGGTGGTGGGCAATCCGG
CCAACACCAACGCCTACATCACCGCCAAGAGCGCTCCGGACCTGCCGGCGAAGAACATCACCGCCATGCTCCGCCTGGAC
CACAACCGCGCCCTGTCGCAGTTCGCGGCCAAGGCCGGCATCGCCACCGCGGACATCGAGAAGGTGGCGGTGTGGGGCAA
CCACTCCCCGACCATGTATGCCGACTACCGCTTCGCCACCGCGAACGGCAAGCCGCTGCCGGAGCTCATCAACGACGAGA
CCTGGTATCGCGAGACCCTGCTTCCCAAGGTGGGCAAGCGCGGCGCCGCCATCATCGAGGCCCGTGGCCTGTCGTCGGCC
GCCTCGGCTGCCAACGCCGCCGTGGACCACATGCATGACTGGATCCACGGCACCGACGGCAAGTGGGTGTCCATGGGCGT
GGCCTCCGACGGCTCCTATGGCGTGCCCGAGGGCATCGTGTTCGGCGTCCCGGCGGTGTGCAGCGGCGGCGAGTATGAAG
TGGTCAAGGGCCTGCCCATGGACGACTTCTCCAAGGCCATGTTCGAGAAGACCCTGAACGAGCTGCTTGAAGAGCGCGAC
GGCGTCGCCGCCCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
CCGAATCGGCTCCGCCTCGCGCCTTTCCTTCCGCCGCGCTGCGGCATAGATTGCGCGCCCGAGCCTTCGGGGCTCGACCT
AAGGCAAAGGGCTTCTTCTC

Downstream 100 bases:

>100_bases
TCTCGTCTGGTCGACGCGGTGCGAATCTGTTTCCCGGACCCCGTCGCTCTGTGCGACCGGGGACCGGGACGCAGTCGAAG
ATGGCATCCCTGCCGGTGCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTKPAVRVAVTGAAGQICYSLLFRIANGDMYGKDQPVILQLLDLPQAQAAVGGVVMELEDCAFPNLAGVVITDDPKVAFK
DIDAAVLVGARPRSKGMERADLLSANAEIFKVQGKALNEVAKRDVKVLVVGNPANTNAYITAKSAPDLPAKNITAMLRLD
HNRALSQFAAKAGIATADIEKVAVWGNHSPTMYADYRFATANGKPLPELINDETWYRETLLPKVGKRGAAIIEARGLSSA
ASAANAAVDHMHDWIHGTDGKWVSMGVASDGSYGVPEGIVFGVPAVCSGGEYEVVKGLPMDDFSKAMFEKTLNELLEERD
GVAALLG

Sequences:

>Translated_327_residues
MTKPAVRVAVTGAAGQICYSLLFRIANGDMYGKDQPVILQLLDLPQAQAAVGGVVMELEDCAFPNLAGVVITDDPKVAFK
DIDAAVLVGARPRSKGMERADLLSANAEIFKVQGKALNEVAKRDVKVLVVGNPANTNAYITAKSAPDLPAKNITAMLRLD
HNRALSQFAAKAGIATADIEKVAVWGNHSPTMYADYRFATANGKPLPELINDETWYRETLLPKVGKRGAAIIEARGLSSA
ASAANAAVDHMHDWIHGTDGKWVSMGVASDGSYGVPEGIVFGVPAVCSGGEYEVVKGLPMDDFSKAMFEKTLNELLEERD
GVAALLG
>Mature_326_residues
TKPAVRVAVTGAAGQICYSLLFRIANGDMYGKDQPVILQLLDLPQAQAAVGGVVMELEDCAFPNLAGVVITDDPKVAFKD
IDAAVLVGARPRSKGMERADLLSANAEIFKVQGKALNEVAKRDVKVLVVGNPANTNAYITAKSAPDLPAKNITAMLRLDH
NRALSQFAAKAGIATADIEKVAVWGNHSPTMYADYRFATANGKPLPELINDETWYRETLLPKVGKRGAAIIEARGLSSAA
SAANAAVDHMHDWIHGTDGKWVSMGVASDGSYGVPEGIVFGVPAVCSGGEYEVVKGLPMDDFSKAMFEKTLNELLEERDG
VAALLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=50, Blast_Score=327, Evalue=8e-90,
Organism=Homo sapiens, GI89886456, Length=334, Percent_Identity=25.1497005988024, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=53.9877300613497, Blast_Score=322, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32566798, Length=263, Percent_Identity=54.7528517110266, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=47.6470588235294, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24583394, Length=331, Percent_Identity=53.4743202416918, Blast_Score=329, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34679; Mature: 34548

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPAVRVAVTGAAGQICYSLLFRIANGDMYGKDQPVILQLLDLPQAQAAVGGVVMELED
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHCCEEEEEHH
CAFPNLAGVVITDDPKVAFKDIDAAVLVGARPRSKGMERADLLSANAEIFKVQGKALNEV
CCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHH
AKRDVKVLVVGNPANTNAYITAKSAPDLPAKNITAMLRLDHNRALSQFAAKAGIATADIE
HHCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCE
KVAVWGNHSPTMYADYRFATANGKPLPELINDETWYRETLLPKVGKRGAAIIEARGLSSA
EEEEECCCCCCEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCHH
ASAANAAVDHMHDWIHGTDGKWVSMGVASDGSYGVPEGIVFGVPAVCSGGEYEVVKGLPM
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEEECCCC
DDFSKAMFEKTLNELLEERDGVAALLG
HHHHHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TKPAVRVAVTGAAGQICYSLLFRIANGDMYGKDQPVILQLLDLPQAQAAVGGVVMELED
CCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHCCEEEEEHH
CAFPNLAGVVITDDPKVAFKDIDAAVLVGARPRSKGMERADLLSANAEIFKVQGKALNEV
CCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHH
AKRDVKVLVVGNPANTNAYITAKSAPDLPAKNITAMLRLDHNRALSQFAAKAGIATADIE
HHCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCE
KVAVWGNHSPTMYADYRFATANGKPLPELINDETWYRETLLPKVGKRGAAIIEARGLSSA
EEEEECCCCCCEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCHH
ASAANAAVDHMHDWIHGTDGKWVSMGVASDGSYGVPEGIVFGVPAVCSGGEYEVVKGLPM
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEEECCCC
DDFSKAMFEKTLNELLEERDGVAALLG
HHHHHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA