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The map label for this gene is pdhC [H]

Identifier: 154245518

GI number: 154245518

Start: 1783406

End: 1784791

Strand: Direct

Name: pdhC [H]

Synonym: Xaut_1572

Alternate gene names: 154245518

Gene position: 1783406-1784791 (Clockwise)

Preceding gene: 154245517

Following gene: 154245519

Centisome position: 33.59

GC content: 72.22

Gene sequence:

>1386_bases
ATGCCCAAGGAAATCCTGATGCCCGCCCTCTCTGCCGGAATGGAGGAGGGTCACCTCGTCCGCTGGCTGAAGAAGGAAGG
CGAGGCGGTGAAGCGTGGTGACCTGCTCGCCGAGATCGAGACCGACAAGGCCGTCATGGAGATGGAGGCGGAGGACGAAG
GCCGCCTCGGCCCCATCCTGATCGGCGACGGTTCGCGCGGCGTGGCGGTGGGCACACTCATCGCCTCCATCCTCGCGGAA
GGGGAAGCCGCGCCCGCGCGGGTCGCGTCCGTCGCCGCCGCGCCGGCCGTGGCGGCGCCTGCGGCGGTTGCTGTTCCTTC
GCCTGCGCCATCGACACCTGCGCCCTTCGCACGGGCGCCCGCTGCGACGGTTGCACCGGCCCCGAGCCTCGCGGCGCGGG
CGCCGTCCATCGCGGCCCTTGTCGCCGCGCGCAAGGTCGTTGCCTCGCCCCTCGCGAGGCGCCTCGCGGACGATTTCGGC
GTCGATCTCAGCACGCTCACCGGCTCAGGTCCCAGGGGCCGCATCGTGCGGATCGACGTGGAGAAGGCGCGCCACCAGCC
GGTTGCGCCCGCCGCGCCGCGTTTTTCCCCTGCTGCGCCGCGTCTTGGCAGCGGGTCGGGGCAGGGGGGACTCAACCATG
CGTGGCTGCGGCAGGGTGAAGGCCGGCCGCTGGTGCTGGTCCACGGCTTCGGCTCGGAGCTGAACGGCTGGCGTCCCTTC
CTTGCCGGCGCTGCGGTGTCGCGGCCGGTGCTCGGCATCGACCTGCCAGGCCATGGCGGATCGGCCGGCCACGGCGCCGC
CCGCTTCGAGGCGCTAGTGGCGGCGGTGGGCGAGACCCTGCAGGGCCTCGGCCTGTTCGATCTGGATCTTGTCGCCCATT
CCCTCGGCGCGGCGGTGGCGGTGGCGCTCGCGGCCGAAGGCTATCCCGATGTGCGCTCCTTGTTCCTGCTCGCGCCGGCG
GGCCTTGGGCCCGATATCAACGGCGCCTTCGTATCCGGCCTTGCCCGCGCCCGCACCGCCGAGAGCCTTGCGCCCTGGCT
GGGTGAACTAGTGGCCGATCCTTCGGTGCTGACCCCCGCCTTCGTGCGGGCCAGCGCCGCGGCCCGTGCCGACGACGCGC
TGGTAGCCGCCCAGCAGCGTCTCGCCGCAGCCCTGTTCCCGGACGGCACGCAGGCCTTCAGCGTGCGCGCGGAGCTGGAG
CAGTTGCGCATTCCGGTCACTGTCGTGGCCGGCACCGAGGATAAGATCATCCCGCCGGGCCATGTGCGGCGGCTGCCTGG
TATGGTGGGCCTGCACCTGTTTCCGGGAGTTGGCCACATGCCGCAGCTGGAGATCCGCGACGAGGTCTGGCGCCTTCTGG
AGCGCCATTTGCGCGCGGCGGGATGA

Upstream 100 bases:

>100_bases
CTGGAAGCCGCCGCCTGCGCCGGTCCCAAGGACATCGAGGCCGGCATCCGTCGCGTCATGGCCGAGCAGGGCCTCGCCCT
CGCCTGATCAGGAGCCCCAC

Downstream 100 bases:

>100_bases
GGCCGCAGAGAGTGAAGAAGAACCGGGTGAAGCCAAGCAAAACCACGTCCCAGGGAGGACTTTTCATGACCATCCGTGCA
CGTTTCCTCGCGCCGATCGC

Product: biotin/lipoyl attachment domain-containing protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPKEILMPALSAGMEEGHLVRWLKKEGEAVKRGDLLAEIETDKAVMEMEAEDEGRLGPILIGDGSRGVAVGTLIASILAE
GEAAPARVASVAAAPAVAAPAAVAVPSPAPSTPAPFARAPAATVAPAPSLAARAPSIAALVAARKVVASPLARRLADDFG
VDLSTLTGSGPRGRIVRIDVEKARHQPVAPAAPRFSPAAPRLGSGSGQGGLNHAWLRQGEGRPLVLVHGFGSELNGWRPF
LAGAAVSRPVLGIDLPGHGGSAGHGAARFEALVAAVGETLQGLGLFDLDLVAHSLGAAVAVALAAEGYPDVRSLFLLAPA
GLGPDINGAFVSGLARARTAESLAPWLGELVADPSVLTPAFVRASAAARADDALVAAQQRLAAALFPDGTQAFSVRAELE
QLRIPVTVVAGTEDKIIPPGHVRRLPGMVGLHLFPGVGHMPQLEIRDEVWRLLERHLRAAG

Sequences:

>Translated_461_residues
MPKEILMPALSAGMEEGHLVRWLKKEGEAVKRGDLLAEIETDKAVMEMEAEDEGRLGPILIGDGSRGVAVGTLIASILAE
GEAAPARVASVAAAPAVAAPAAVAVPSPAPSTPAPFARAPAATVAPAPSLAARAPSIAALVAARKVVASPLARRLADDFG
VDLSTLTGSGPRGRIVRIDVEKARHQPVAPAAPRFSPAAPRLGSGSGQGGLNHAWLRQGEGRPLVLVHGFGSELNGWRPF
LAGAAVSRPVLGIDLPGHGGSAGHGAARFEALVAAVGETLQGLGLFDLDLVAHSLGAAVAVALAAEGYPDVRSLFLLAPA
GLGPDINGAFVSGLARARTAESLAPWLGELVADPSVLTPAFVRASAAARADDALVAAQQRLAAALFPDGTQAFSVRAELE
QLRIPVTVVAGTEDKIIPPGHVRRLPGMVGLHLFPGVGHMPQLEIRDEVWRLLERHLRAAG
>Mature_460_residues
PKEILMPALSAGMEEGHLVRWLKKEGEAVKRGDLLAEIETDKAVMEMEAEDEGRLGPILIGDGSRGVAVGTLIASILAEG
EAAPARVASVAAAPAVAAPAAVAVPSPAPSTPAPFARAPAATVAPAPSLAARAPSIAALVAARKVVASPLARRLADDFGV
DLSTLTGSGPRGRIVRIDVEKARHQPVAPAAPRFSPAAPRLGSGSGQGGLNHAWLRQGEGRPLVLVHGFGSELNGWRPFL
AGAAVSRPVLGIDLPGHGGSAGHGAARFEALVAAVGETLQGLGLFDLDLVAHSLGAAVAVALAAEGYPDVRSLFLLAPAG
LGPDINGAFVSGLARARTAESLAPWLGELVADPSVLTPAFVRASAAARADDALVAAQQRLAAALFPDGTQAFSVRAELEQ
LRIPVTVVAGTEDKIIPPGHVRRLPGMVGLHLFPGVGHMPQLEIRDEVWRLLERHLRAAG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098753, Length=181, Percent_Identity=37.5690607734807, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI203098816, Length=181, Percent_Identity=37.5690607734807, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI31711992, Length=183, Percent_Identity=38.7978142076503, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=62.7118644067797, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560088, Length=189, Percent_Identity=42.3280423280423, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324258, Length=179, Percent_Identity=34.6368715083799, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321632, Length=187, Percent_Identity=32.0855614973262, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129315, Length=72, Percent_Identity=52.7777777777778, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582497, Length=65, Percent_Identity=52.3076923076923, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47184; Mature: 47053

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEILMPALSAGMEEGHLVRWLKKEGEAVKRGDLLAEIETDKAVMEMEAEDEGRLGPIL
CCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCEEEEECCCHHHHEECCCCCCCCCCEE
IGDGSRGVAVGTLIASILAEGEAAPARVASVAAAPAVAAPAAVAVPSPAPSTPAPFARAP
ECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCEEECCCCCCCCCCCCCCCC
AATVAPAPSLAARAPSIAALVAARKVVASPLARRLADDFGVDLSTLTGSGPRGRIVRIDV
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEH
EKARHQPVAPAAPRFSPAAPRLGSGSGQGGLNHAWLRQGEGRPLVLVHGFGSELNGWRPF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHH
LAGAAVSRPVLGIDLPGHGGSAGHGAARFEALVAAVGETLQGLGLFDLDLVAHSLGAAVA
HHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VALAAEGYPDVRSLFLLAPAGLGPDINGAFVSGLARARTAESLAPWLGELVADPSVLTPA
HHHHHCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVRASAAARADDALVAAQQRLAAALFPDGTQAFSVRAELEQLRIPVTVVAGTEDKIIPPG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
HVRRLPGMVGLHLFPGVGHMPQLEIRDEVWRLLERHLRAAG
HHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKEILMPALSAGMEEGHLVRWLKKEGEAVKRGDLLAEIETDKAVMEMEAEDEGRLGPIL
CHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCEEEEECCCHHHHEECCCCCCCCCCEE
IGDGSRGVAVGTLIASILAEGEAAPARVASVAAAPAVAAPAAVAVPSPAPSTPAPFARAP
ECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCEEECCCCCCCCCCCCCCCC
AATVAPAPSLAARAPSIAALVAARKVVASPLARRLADDFGVDLSTLTGSGPRGRIVRIDV
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEH
EKARHQPVAPAAPRFSPAAPRLGSGSGQGGLNHAWLRQGEGRPLVLVHGFGSELNGWRPF
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHH
LAGAAVSRPVLGIDLPGHGGSAGHGAARFEALVAAVGETLQGLGLFDLDLVAHSLGAAVA
HHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VALAAEGYPDVRSLFLLAPAGLGPDINGAFVSGLARARTAESLAPWLGELVADPSVLTPA
HHHHHCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVRASAAARADDALVAAQQRLAAALFPDGTQAFSVRAELEQLRIPVTVVAGTEDKIIPPG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
HVRRLPGMVGLHLFPGVGHMPQLEIRDEVWRLLERHLRAAG
HHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA