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The map label for this gene is cspA [C]

Identifier: 154245503

GI number: 154245503

Start: 1767984

End: 1768196

Strand: Direct

Name: cspA [C]

Synonym: Xaut_1556

Alternate gene names: 154245503

Gene position: 1767984-1768196 (Clockwise)

Preceding gene: 154245501

Following gene: 154245504

Centisome position: 33.3

GC content: 59.15

Gene sequence:

>213_bases
ATGGCTGAGGGAACCGTAAAGTGGTTCAACGCCCAGAAGGGCTTTGGCTTCATCACTCCCGATTCTGGTGGCAGCGATGC
CTTCGTGCATATCAGCGCGGTTGAGCGCGCAGGTATGTCGGACCTGCGCGAAGGTCAGAAGGTGGCCTTCGAGCTCGTCA
CGGATCGCAAGAGCGGCAAGCTGTCCGCGGATCAGCTCAAGGATCTGAGCTGA

Upstream 100 bases:

>100_bases
TGATTTCTGCTCTAAACCAATATCTCTCGCCAGAAAATGCGATTTCGACGGCCGGGCAGGGCTCGGCAAGCTACAACTCA
AAAATTTGAAAGGGTTTCCC

Downstream 100 bases:

>100_bases
TCTTTTCGGCCGGCGGCCCGGTCACCTGGTCATCGCCGGCCTTCGCATCGCTTTCATGAGAGATGCGTAGACCACACTTC
TGCAAGGCGCCCGGCGCCTT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MAEGTVKWFNAQKGFGFITPDSGGSDAFVHISAVERAGMSDLREGQKVAFELVTDRKSGKLSADQLKDLS

Sequences:

>Translated_70_residues
MAEGTVKWFNAQKGFGFITPDSGGSDAFVHISAVERAGMSDLREGQKVAFELVTDRKSGKLSADQLKDLS
>Mature_69_residues
AEGTVKWFNAQKGFGFITPDSGGSDAFVHISAVERAGMSDLREGQKVAFELVTDRKSGKLSADQLKDLS

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=49, Percent_Identity=63.265306122449, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1786841, Length=55, Percent_Identity=54.5454545454545, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI2367114, Length=49, Percent_Identity=57.1428571428571, Blast_Score=64, Evalue=1e-12,
Organism=Escherichia coli, GI1788126, Length=55, Percent_Identity=54.5454545454545, Blast_Score=64, Evalue=1e-12,
Organism=Escherichia coli, GI1787107, Length=52, Percent_Identity=51.9230769230769, Blast_Score=63, Evalue=4e-12,
Organism=Escherichia coli, GI1787839, Length=49, Percent_Identity=57.1428571428571, Blast_Score=62, Evalue=6e-12,
Organism=Escherichia coli, GI1787834, Length=47, Percent_Identity=55.3191489361702, Blast_Score=59, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7535; Mature: 7403

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGTVKWFNAQKGFGFITPDSGGSDAFVHISAVERAGMSDLREGQKVAFELVTDRKSGK
CCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHCCHHEEEHEECCCCCCC
LSADQLKDLS
CCHHHHCCCC
>Mature Secondary Structure 
AEGTVKWFNAQKGFGFITPDSGGSDAFVHISAVERAGMSDLREGQKVAFELVTDRKSGK
CCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHCCHHEEEHEECCCCCCC
LSADQLKDLS
CCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]