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The map label for this gene is ygbJ [H]

Identifier: 154245485

GI number: 154245485

Start: 1748219

End: 1749097

Strand: Reverse

Name: ygbJ [H]

Synonym: Xaut_1538

Alternate gene names: 154245485

Gene position: 1749097-1748219 (Counterclockwise)

Preceding gene: 154245486

Following gene: 154245484

Centisome position: 32.95

GC content: 72.58

Gene sequence:

>879_bases
ATGACGACCATCGGCTTCATCGGTCTCGGCTCCATGGGCCTGCCCATGGCGCGCAACCTCGTGGCGCGGGGCTTCGAGGT
CACCGGCTTCGACCTGCGCGCCGAGGCCCGCAACGCCCTCGTGGCGGCGGGCGGCAAGGCTGCCGACACCGCCGCGGCCG
CCTGCGCCGGGGCGGACATGGCCGTGCTGATGGTGGTGAACATCGCCCAGGCCCGCGACGTGCTGTTCACTGGCGGCGTG
CTCGATGCTCTCGCCCCCGGCGCTGCGGTCCTGCTCATGTCCACCTGCCCGCCGGCAGCGGTGGCCGAGGTGGCGGCAGA
GGTGGAAGCGCGGGGCTTCACCTTCGTCGATGCCCCGGTTTCGGGCGGAGTGGTGGGCGCGACCGCCGGCTCCCTCACCA
TCATGGTGGGCGCCGGCGATGCCGCTTTCGCCCGCGCCAAGCCGGCGCTCGGCGCCATGGGCGCCAAGGTGGTGCATGTG
GGCAAGGACGCTGGACAGGGCGCCACCGCGAAGGTGGTGAACCAGCTGCTGTGCGGCGCCCATCTCGCCGTCGCGGCGGA
AGCCCTGTCGCTGGCCGAGCGGCTGGGCGTGGACACCACCGCCATGCTCGACATTCTCGGCGGATCGGCGGCTGCAAGCT
GGATGCTGCGCGACCGCGGTCCCCGCATGCTGGAAGCCGACCCCACCGTCACCAGCGCCGTGGACATCTTCGTGAAGGAT
CTTGGCCTCGTGCTGGAGGCCGGACGCGAGGCCAAGGCCGCCCTGCCCATGGCAGCCCTCGCCCACCAGATGTTCCTCTC
CGTCTCCGGCCGCGGCCTCGGCGCCGCCGACGACAGCCAGGTCATCCGCGCCTATCGCAGCCTGCAGGGCCGGGACTGA

Upstream 100 bases:

>100_bases
GGGCCCGCCCCTTCCCGCCCGATCCGCCGGAGAGCGGACGGTCCAGGCAAACGAAATCGCCCAAGAAAGACATGGCCCAA
AGCGGCCTAGGGGAGGAAGA

Downstream 100 bases:

>100_bases
CCCGCCGGCGCCTCCGCCGACACGCCAAACGCCGACCACCCAAGCGCCGACCGCCAACGCGCGGAACATCAAGAAGGCCG
CACAGCGGCCATAAGGGAGA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTTIGFIGLGSMGLPMARNLVARGFEVTGFDLRAEARNALVAAGGKAADTAAAACAGADMAVLMVVNIAQARDVLFTGGV
LDALAPGAAVLLMSTCPPAAVAEVAAEVEARGFTFVDAPVSGGVVGATAGSLTIMVGAGDAAFARAKPALGAMGAKVVHV
GKDAGQGATAKVVNQLLCGAHLAVAAEALSLAERLGVDTTAMLDILGGSAAASWMLRDRGPRMLEADPTVTSAVDIFVKD
LGLVLEAGREAKAALPMAALAHQMFLSVSGRGLGAADDSQVIRAYRSLQGRD

Sequences:

>Translated_292_residues
MTTIGFIGLGSMGLPMARNLVARGFEVTGFDLRAEARNALVAAGGKAADTAAAACAGADMAVLMVVNIAQARDVLFTGGV
LDALAPGAAVLLMSTCPPAAVAEVAAEVEARGFTFVDAPVSGGVVGATAGSLTIMVGAGDAAFARAKPALGAMGAKVVHV
GKDAGQGATAKVVNQLLCGAHLAVAAEALSLAERLGVDTTAMLDILGGSAAASWMLRDRGPRMLEADPTVTSAVDIFVKD
LGLVLEAGREAKAALPMAALAHQMFLSVSGRGLGAADDSQVIRAYRSLQGRD
>Mature_291_residues
TTIGFIGLGSMGLPMARNLVARGFEVTGFDLRAEARNALVAAGGKAADTAAAACAGADMAVLMVVNIAQARDVLFTGGVL
DALAPGAAVLLMSTCPPAAVAEVAAEVEARGFTFVDAPVSGGVVGATAGSLTIMVGAGDAAFARAKPALGAMGAKVVHVG
KDAGQGATAKVVNQLLCGAHLAVAAEALSLAERLGVDTTAMLDILGGSAAASWMLRDRGPRMLEADPTVTSAVDIFVKDL
GLVLEAGREAKAALPMAALAHQMFLSVSGRGLGAADDSQVIRAYRSLQGRD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=33.6734693877551, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=29.6819787985866, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=40.7665505226481, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI145693186, Length=286, Percent_Identity=33.2167832167832, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=32.3943661971831, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=31.9444444444444, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=32, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24655230, Length=287, Percent_Identity=31.0104529616725, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922568, Length=287, Percent_Identity=31.0104529616725, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=24.113475177305, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 29142; Mature: 29011

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIGFIGLGSMGLPMARNLVARGFEVTGFDLRAEARNALVAAGGKAADTAAAACAGADM
CCEEEEEECCCCCCHHHHHHHHCCCEEECEEEHHHHCCEEEECCCCCHHHHHHHHCCCCH
AVLMVVNIAQARDVLFTGGVLDALAPGAAVLLMSTCPPAAVAEVAAEVEARGFTFVDAPV
HHHHHHHHHHHHHHEEECCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCC
SGGVVGATAGSLTIMVGAGDAAFARAKPALGAMGAKVVHVGKDAGQGATAKVVNQLLCGA
CCCEEEECCCCEEEEEECCCHHHHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
HLAVAAEALSLAERLGVDTTAMLDILGGSAAASWMLRDRGPRMLEADPTVTSAVDIFVKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
LGLVLEAGREAKAALPMAALAHQMFLSVSGRGLGAADDSQVIRAYRSLQGRD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIGFIGLGSMGLPMARNLVARGFEVTGFDLRAEARNALVAAGGKAADTAAAACAGADM
CEEEEEECCCCCCHHHHHHHHCCCEEECEEEHHHHCCEEEECCCCCHHHHHHHHCCCCH
AVLMVVNIAQARDVLFTGGVLDALAPGAAVLLMSTCPPAAVAEVAAEVEARGFTFVDAPV
HHHHHHHHHHHHHHEEECCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCC
SGGVVGATAGSLTIMVGAGDAAFARAKPALGAMGAKVVHVGKDAGQGATAKVVNQLLCGA
CCCEEEECCCCEEEEEECCCHHHHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
HLAVAAEALSLAERLGVDTTAMLDILGGSAAASWMLRDRGPRMLEADPTVTSAVDIFVKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
LGLVLEAGREAKAALPMAALAHQMFLSVSGRGLGAADDSQVIRAYRSLQGRD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]