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The map label for this gene is ilvI [H]

Identifier: 154245435

GI number: 154245435

Start: 1697125

End: 1698930

Strand: Direct

Name: ilvI [H]

Synonym: Xaut_1488

Alternate gene names: 154245435

Gene position: 1697125-1698930 (Clockwise)

Preceding gene: 154245427

Following gene: 154245436

Centisome position: 31.97

GC content: 66.33

Gene sequence:

>1806_bases
GTGAGCCCGGCGGGGCCGAAGGCGCCCCTGATGGAGTTGCAGACCATGAGCATCCAGATGACCGGCGCCGAAATGGTGAT
GCGCGCCTTCGCCGACCAGGGGGTCGAGCATTTCTTCGGCTATCCGGGCGGGGCGGTGCTGCCGATCTATGACGCGCTGT
TCCAGCAGAACGCCATCGAGCACATCCTGGTGCGCCACGAGCAGGCGGCGGTCCATGCGGCGGAGGGCTATGCCCGCTCC
TCCGGCAAGGTCGGCGTGGTGCTGGTCACCTCCGGCCCCGGCGCGACCAATGCGGTCACCGGCCTCACCGACGCGCTGAT
GGATTCGATTCCCCTCGTCTGCATCACCGGGCAGGTGGCGACCCACCTCATCGGCAATGACGCGTTCCAGGAATGCGACA
CGGTGGGCATCACCCGGCCCTGCACCAAGCACAATTACCTCGTGCGCGACGTGAACGACCTCGCACGCGTGCTGCACGAG
GCCTTCTACGTGGCGCAGAACGGCCGGCCCGGCCCGGTGGTCGTCGACATTCCCAAGGATGTCCAGTTCGCCACCGGCAC
CTACATCGGCCGCGAGAACATCCAGCACAAGACCTACCGCCCCAAGCTGAAGGCCGAGGACGCGCAGATCTCCGCCGCGG
TGGAGATGATCGCGGGCGCGAAGAAGCCGATCTTCTACACCGGCGGCGGCGTCATCAATTCCGGGCCGGAGGCGAGCCGC
CTGCTGCGCGAGCTGGCGCGCCTCACCGGCGTGCCGGTCACCTCGACGCTGATGGGCCTCGGCGCCTTCCCGGCGGCGAA
CCGGCAGTGGCTGGGCATGCTGGGCATGCACGGCACCTATGAAGCCAACATGGCCATGCACGATTGCGACGTGATGGTCT
GCATCGGCGCGCGCTTCGACGACCGCATCACCGGCCGCCTCGACGCCTTCGCGCCGGGCTCGAAGAAGATCCACATCGAC
ATCGATCCCTCCTCCATCAACAAGAACGTGAAGGTGGACCTGCCCATCATCGGCGACTGCACCCGCGTGCTCGCCGACAT
GGTCGAGGCCTGGAACGCAAGGTCGCTGAAGATCGACCAGCCGGCGCTCGCCGCCTGGTGGACGCAGATCGAGCGCTGGC
GCTCGCGCAACTGCCTCGCCTATCGCCCGTCCGACGAGATCATCAAGCCGCAGTTCGCCATCGAGCGGCTGTACGAGGCG
ACCAAGGACAAGGACGTCTACATCTCCACGGAAGTGGGCCAGCACCAGATGTGGGCGGCGCAGTTCTTCCGCTTCGAGGA
GCCGAACCGCTGGATGACCTCCGGCGGCCTCGGCACCATGGGCTACGGCCTGCCCGCCGCCATCGGCGCGCAGAAGGCCC
ACCCGAACGCGCTGTGCATCGACATCGCGGGCGAGGCCTCCATCCTCATGAACATGCAGGAGATGTCCACGGCGGTGCAG
TTCGACCTGCCGGTGAAGATCTTCATCCTCAACAACCGCTACATGGGCATGGTGCGCCAGTGGCAGGAACTGCTGCACGG
CGGGCGCTATTCCCACTCCTATTCGGAAGCGCTGCCCGACTTCGTGAAGCTGGCCGACGCCTACGGCGGCGTGGGCATCC
GCTGCGAGAAGCCCGGCGACCTCGACGCGGCGATCCAGGAGATGATCAACGTGAAGCGGCCGGTGATCTTCGACTGCATC
GTGGACCAGCAGGAGAATTGCTTCCCCATGATCCCGTCGGGCCGCGCCCACAACGAGATGATCCTCGGCGACATGGCCAT
CGACCTCGACGATGCCATCACCGACGAGGGCAAGATGCTGGTCTGA

Upstream 100 bases:

>100_bases
TTCTGAAGGGCCTCGGCCCTGAGGGCTTCGGCGTCCGGCACCGGCCGCCATTCGTGACCGAGATGCCGGCAAGGCCGGCG
TTGATGCAGGATTTGCCGGC

Downstream 100 bases:

>100_bases
GGCACGGTCGCGAGCGGGGCGGCACGCGCTGACACGCGCCGCTCTTGCCCCTCTCCCGCTCGCGGGGGAGGGGCGGGGCG
GGCGCAGACCCTGTTCGCCA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSPAGPKAPLMELQTMSIQMTGAEMVMRAFADQGVEHFFGYPGGAVLPIYDALFQQNAIEHILVRHEQAAVHAAEGYARS
SGKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHE
AFYVAQNGRPGPVVVDIPKDVQFATGTYIGRENIQHKTYRPKLKAEDAQISAAVEMIAGAKKPIFYTGGGVINSGPEASR
LLRELARLTGVPVTSTLMGLGAFPAANRQWLGMLGMHGTYEANMAMHDCDVMVCIGARFDDRITGRLDAFAPGSKKIHID
IDPSSINKNVKVDLPIIGDCTRVLADMVEAWNARSLKIDQPALAAWWTQIERWRSRNCLAYRPSDEIIKPQFAIERLYEA
TKDKDVYISTEVGQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQKAHPNALCIDIAGEASILMNMQEMSTAVQ
FDLPVKIFILNNRYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDAAIQEMINVKRPVIFDCI
VDQQENCFPMIPSGRAHNEMILGDMAIDLDDAITDEGKMLV

Sequences:

>Translated_601_residues
MSPAGPKAPLMELQTMSIQMTGAEMVMRAFADQGVEHFFGYPGGAVLPIYDALFQQNAIEHILVRHEQAAVHAAEGYARS
SGKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHE
AFYVAQNGRPGPVVVDIPKDVQFATGTYIGRENIQHKTYRPKLKAEDAQISAAVEMIAGAKKPIFYTGGGVINSGPEASR
LLRELARLTGVPVTSTLMGLGAFPAANRQWLGMLGMHGTYEANMAMHDCDVMVCIGARFDDRITGRLDAFAPGSKKIHID
IDPSSINKNVKVDLPIIGDCTRVLADMVEAWNARSLKIDQPALAAWWTQIERWRSRNCLAYRPSDEIIKPQFAIERLYEA
TKDKDVYISTEVGQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQKAHPNALCIDIAGEASILMNMQEMSTAVQ
FDLPVKIFILNNRYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDAAIQEMINVKRPVIFDCI
VDQQENCFPMIPSGRAHNEMILGDMAIDLDDAITDEGKMLV
>Mature_600_residues
SPAGPKAPLMELQTMSIQMTGAEMVMRAFADQGVEHFFGYPGGAVLPIYDALFQQNAIEHILVRHEQAAVHAAEGYARSS
GKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCITGQVATHLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEA
FYVAQNGRPGPVVVDIPKDVQFATGTYIGRENIQHKTYRPKLKAEDAQISAAVEMIAGAKKPIFYTGGGVINSGPEASRL
LRELARLTGVPVTSTLMGLGAFPAANRQWLGMLGMHGTYEANMAMHDCDVMVCIGARFDDRITGRLDAFAPGSKKIHIDI
DPSSINKNVKVDLPIIGDCTRVLADMVEAWNARSLKIDQPALAAWWTQIERWRSRNCLAYRPSDEIIKPQFAIERLYEAT
KDKDVYISTEVGQHQMWAAQFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQKAHPNALCIDIAGEASILMNMQEMSTAVQF
DLPVKIFILNNRYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGDLDAAIQEMINVKRPVIFDCIV
DQQENCFPMIPSGRAHNEMILGDMAIDLDDAITDEGKMLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=25.1327433628319, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=515, Percent_Identity=25.631067961165, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=52.112676056338, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=42.756183745583, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=33.220910623946, Blast_Score=307, Evalue=2e-84,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=27.5491949910555, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=24.1258741258741, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=25.3886010362694, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=25.3886010362694, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=517, Percent_Identity=26.1121856866538, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=44.1471571906354, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320816, Length=505, Percent_Identity=23.5643564356436, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=26.1855670103093, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65891; Mature: 65759

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAGPKAPLMELQTMSIQMTGAEMVMRAFADQGVEHFFGYPGGAVLPIYDALFQQNAIE
CCCCCCCCCHHEEEEEEEEECHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHH
HILVRHEQAAVHAAEGYARSSGKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCITGQVA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECHHH
THLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGPVVVDIPKD
HHHHCCHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
VQFATGTYIGRENIQHKTYRPKLKAEDAQISAAVEMIAGAKKPIFYTGGGVINSGPEASR
CEEECCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
LLRELARLTGVPVTSTLMGLGAFPAANRQWLGMLGMHGTYEANMAMHDCDVMVCIGARFD
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHCCCCCCEECCEEEECCCEEEEECCCCC
DRITGRLDAFAPGSKKIHIDIDPSSINKNVKVDLPIIGDCTRVLADMVEAWNARSLKIDQ
CCCCCCEECCCCCCCEEEEEECHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECC
PALAAWWTQIERWRSRNCLAYRPSDEIIKPQFAIERLYEATKDKDVYISTEVGQHQMWAA
CHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
QFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQKAHPNALCIDIAGEASILMNMQEMSTAVQ
HHHHCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCHHEEEEHHHHHHHEE
FDLPVKIFILNNRYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGD
ECCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCC
LDAAIQEMINVKRPVIFDCIVDQQENCFPMIPSGRAHNEMILGDMAIDLDDAITDEGKML
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCC
V
C
>Mature Secondary Structure 
SPAGPKAPLMELQTMSIQMTGAEMVMRAFADQGVEHFFGYPGGAVLPIYDALFQQNAIE
CCCCCCCCHHEEEEEEEEECHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHH
HILVRHEQAAVHAAEGYARSSGKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCITGQVA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECHHH
THLIGNDAFQECDTVGITRPCTKHNYLVRDVNDLARVLHEAFYVAQNGRPGPVVVDIPKD
HHHHCCHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
VQFATGTYIGRENIQHKTYRPKLKAEDAQISAAVEMIAGAKKPIFYTGGGVINSGPEASR
CEEECCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
LLRELARLTGVPVTSTLMGLGAFPAANRQWLGMLGMHGTYEANMAMHDCDVMVCIGARFD
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHCCCCCCEECCEEEECCCEEEEECCCCC
DRITGRLDAFAPGSKKIHIDIDPSSINKNVKVDLPIIGDCTRVLADMVEAWNARSLKIDQ
CCCCCCEECCCCCCCEEEEEECHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECC
PALAAWWTQIERWRSRNCLAYRPSDEIIKPQFAIERLYEATKDKDVYISTEVGQHQMWAA
CHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
QFFRFEEPNRWMTSGGLGTMGYGLPAAIGAQKAHPNALCIDIAGEASILMNMQEMSTAVQ
HHHHCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCHHEEEEHHHHHHHEE
FDLPVKIFILNNRYMGMVRQWQELLHGGRYSHSYSEALPDFVKLADAYGGVGIRCEKPGD
ECCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCC
LDAAIQEMINVKRPVIFDCIVDQQENCFPMIPSGRAHNEMILGDMAIDLDDAITDEGKML
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]