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The map label for this gene is aes [H]

Identifier: 154245399

GI number: 154245399

Start: 1665675

End: 1666631

Strand: Direct

Name: aes [H]

Synonym: Xaut_1452

Alternate gene names: 154245399

Gene position: 1665675-1666631 (Clockwise)

Preceding gene: 154245395

Following gene: 154245404

Centisome position: 31.37

GC content: 69.7

Gene sequence:

>957_bases
ATGTCGAAGCTGGTTCTTGATGCCGCCGTGTTCTCGCCGGCGGCCGTCGCGCCCGATACCCGCATGGTCAACGACGCCAT
CGTCAAGATCATGAAGACCATTCCTGATCGCTGGGCGGTGCCGCTACCGGAGATCCGCGCCGCGCGGCTGTCCGGCAAGG
GCGTGTTCCCGCTCGCGCCGGAAAGCGCGCGGGCGCGGCAGGAGACCATTCCCGGCCCGCGCGGGGATATTCCGGTGCGC
ATCTACATGCCGGATGGCGCCCCCACCGGCGTCTACCTGCACATCCACGGCGGCGGCTGGACCGTGGGCTCGGCGCGGGA
GAACGAGGGGATGCTGGACCGCATCGCCGGCCAGTGCGGGCTGGCCGTGGTCTCGGTGGACTACGCGCTCGCCCCCGAGG
AGCCCTATCCCGCCGGCCCGGATGATTGCGAGGCGGCGGCGCTGTGGCTGGTGAAGGAGGGCGCGGCCCGCTTCGGCACC
GAGCGTTTCGCCATCGGCGGCGAATCGGGCGGGGCGACGCTCGCGGTGACGACGCTGGTGCGGCTGCGCGACAAGCACCA
GCTTGCGCCGTTCTCGGCGGCGATGCTGACGGCCGGCTGCTTCGACCTGCGCCTGACCCCCTCCGCGCGCAACTGGGGCG
ACGAGCCGCTGGTGCTGAACACGCGGGACATCACCTTCTTCGTGAACAATTATCTTCGGCTGGAGGGCAAGGCCGAGGAG
GCGGATGTCTCCCCGCTGCTCGGCAACCTGAAGGGCCTGCCGAAGGCGCTGTTCATGGTGGGCACGCGCGACGCGCTGCT
CGACGACACCCTGTTCATGGCCAACGCCTGGCTCGCGGCCGGCAACGTGGCGGAGCTCGATGTCTATCCCGGCGGCTGCC
ACGTCTTCATCGGCTTTCCCGGTACCCTCGCCGATCAGGGCCTCAAGCGCATGTGCGCCTATCTGAGCGCGTTCTGA

Upstream 100 bases:

>100_bases
CGTCTTGCGCCGAGCGGTTCCGGCTCGTACCAGTTTTGCCATCTCCCCGTCTTTATCGCGACTGCGGCCGGCTGGCCGCC
GCCGCCAGGGACTGTCGCCG

Downstream 100 bases:

>100_bases
ACGCCGGTCAGAGGCGCTCCTCGCCGGTTGGGGCGCGCAACTCGTCGATCAGGCCTTTGAGGCGCCGGTAGAAGCGCTCC
GCATAGGCGAAGGCCTGGTC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSKLVLDAAVFSPAAVAPDTRMVNDAIVKIMKTIPDRWAVPLPEIRAARLSGKGVFPLAPESARARQETIPGPRGDIPVR
IYMPDGAPTGVYLHIHGGGWTVGSARENEGMLDRIAGQCGLAVVSVDYALAPEEPYPAGPDDCEAAALWLVKEGAARFGT
ERFAIGGESGGATLAVTTLVRLRDKHQLAPFSAAMLTAGCFDLRLTPSARNWGDEPLVLNTRDITFFVNNYLRLEGKAEE
ADVSPLLGNLKGLPKALFMVGTRDALLDDTLFMANAWLAAGNVAELDVYPGGCHVFIGFPGTLADQGLKRMCAYLSAF

Sequences:

>Translated_318_residues
MSKLVLDAAVFSPAAVAPDTRMVNDAIVKIMKTIPDRWAVPLPEIRAARLSGKGVFPLAPESARARQETIPGPRGDIPVR
IYMPDGAPTGVYLHIHGGGWTVGSARENEGMLDRIAGQCGLAVVSVDYALAPEEPYPAGPDDCEAAALWLVKEGAARFGT
ERFAIGGESGGATLAVTTLVRLRDKHQLAPFSAAMLTAGCFDLRLTPSARNWGDEPLVLNTRDITFFVNNYLRLEGKAEE
ADVSPLLGNLKGLPKALFMVGTRDALLDDTLFMANAWLAAGNVAELDVYPGGCHVFIGFPGTLADQGLKRMCAYLSAF
>Mature_317_residues
SKLVLDAAVFSPAAVAPDTRMVNDAIVKIMKTIPDRWAVPLPEIRAARLSGKGVFPLAPESARARQETIPGPRGDIPVRI
YMPDGAPTGVYLHIHGGGWTVGSARENEGMLDRIAGQCGLAVVSVDYALAPEEPYPAGPDDCEAAALWLVKEGAARFGTE
RFAIGGESGGATLAVTTLVRLRDKHQLAPFSAAMLTAGCFDLRLTPSARNWGDEPLVLNTRDITFFVNNYLRLEGKAEEA
DVSPLLGNLKGLPKALFMVGTRDALLDDTLFMANAWLAAGNVAELDVYPGGCHVFIGFPGTLADQGLKRMCAYLSAF

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=101, Percent_Identity=35.6435643564356, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786682, Length=264, Percent_Identity=24.6212121212121, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=269, Percent_Identity=26.0223048327138, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=109, Percent_Identity=34.8623853211009, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=112, Percent_Identity=34.8214285714286, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33923; Mature: 33792

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLDAAVFSPAAVAPDTRMVNDAIVKIMKTIPDRWAVPLPEIRAARLSGKGVFPLAP
CCCEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHCCCCCCCCCCC
ESARARQETIPGPRGDIPVRIYMPDGAPTGVYLHIHGGGWTVGSARENEGMLDRIAGQCG
CHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCHHHHHHHHCC
LAVVSVDYALAPEEPYPAGPDDCEAAALWLVKEGAARFGTERFAIGGESGGATLAVTTLV
EEEEEEEEEECCCCCCCCCCCCHHHHEEEEEHHCHHHCCCCEEEECCCCCCCEEHHHHHH
RLRDKHQLAPFSAAMLTAGCFDLRLTPSARNWGDEPLVLNTRDITFFVNNYLRLEGKAEE
HHHHCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCEEEEEEECEEEECCCCCC
ADVSPLLGNLKGLPKALFMVGTRDALLDDTLFMANAWLAAGNVAELDVYPGGCHVFIGFP
CCCHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEEECC
GTLADQGLKRMCAYLSAF
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLVLDAAVFSPAAVAPDTRMVNDAIVKIMKTIPDRWAVPLPEIRAARLSGKGVFPLAP
CCEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHCCCCCCCCCCC
ESARARQETIPGPRGDIPVRIYMPDGAPTGVYLHIHGGGWTVGSARENEGMLDRIAGQCG
CHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCHHHHHHHHCC
LAVVSVDYALAPEEPYPAGPDDCEAAALWLVKEGAARFGTERFAIGGESGGATLAVTTLV
EEEEEEEEEECCCCCCCCCCCCHHHHEEEEEHHCHHHCCCCEEEECCCCCCCEEHHHHHH
RLRDKHQLAPFSAAMLTAGCFDLRLTPSARNWGDEPLVLNTRDITFFVNNYLRLEGKAEE
HHHHCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCEEEEEEECEEEECCCCCC
ADVSPLLGNLKGLPKALFMVGTRDALLDDTLFMANAWLAAGNVAELDVYPGGCHVFIGFP
CCCHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEEECC
GTLADQGLKRMCAYLSAF
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA