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The map label for this gene is panB

Identifier: 154245389

GI number: 154245389

Start: 1654392

End: 1655234

Strand: Reverse

Name: panB

Synonym: Xaut_1442

Alternate gene names: 154245389

Gene position: 1655234-1654392 (Counterclockwise)

Preceding gene: 154245390

Following gene: 154245388

Centisome position: 31.18

GC content: 69.99

Gene sequence:

>843_bases
ATGTCCAGTCAGGCCGATGCCCGCCGCATCACGGCGCCGCAGATCCAGGCGCGCAAGGGGGGCGAGCCCATCGTCTCCCT
CACCTCCTACCATTCCCACACGGCACGGCTGCTCGACCGCCATGTGGACGTGATCCTGGTGGGCGACAGCCTCGGCATGG
TGATGCACGGCATGGAGACCACCGTGCCGGTCACCGTGGAGATGATGATCGTCCACGGCCGCGCCGTGGTGCGCGGCACC
AAGCGCGCCCTCATCGTGGTGGACCTGCCCTTCGGCAGCTACGAGGCGAGCCATACGGAAGCCTTCCACACCGCCGCCCG
GGTGCTGAAGGAGACCGGCGCCGGCGCGGTGAAGCTGGAGGGCGGCCGCCGCATGGCCGAGACCGTGCGCTTCCTGGTGG
ATCGCGGCGTGCCGGTGATGGGCCATGTGGGCCTCACCCCCCAGGCCATCAACACCCTGGGCTCCTTCAAGGCCCGCGGC
CGGGACGATGCGGAAGCCTCCATCATCCTCGATGACGCCCGCGCCATCGCCGAGGCCGGCGCTTTCTCCATCGTGCTCGA
AGCCATCGCCGAGCCGCTGGCCCGCCGCATCACCCAGGAGGTGGCGCCCCCGACCATCGGCATCGGCGGCTCGCCCGCCT
GCGACGGCCAGATCCTGGTGCTGGAGGACATGCTGGGTCTTGGCGACCGGGTGCCCAAGTTCGTGAAGAAATACGCCAAT
CTCGGCCCCGACATCGAGAAGGCGGTGGCAAGCTACGCCGACGAGGTGCGCGCCCGCACCTTCCCCGCCGCCGAGCACAC
CTACGCCCCGCGCCTCGTCGAGAAGCCGGCGAAAGCCTCCTGA

Upstream 100 bases:

>100_bases
GCTGGTGCTCTATGCCGCGCTCGCTTACCTATTCCATCCCTATGTGATCGGCGTGCCCGTGATGTGAGGCCGCCTGCGCC
AGAAACGTAAGAGGAACGCC

Downstream 100 bases:

>100_bases
TGGCTGCGCGCCGGACCGGAGCCGCCCTGTCGCGCGCCGGGCTCGGCCTTCTCCTCGCCCTCGCCGCCGCGATATCCGCC
GCGCCGGCGCGGGCGGCGGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSSQADARRITAPQIQARKGGEPIVSLTSYHSHTARLLDRHVDVILVGDSLGMVMHGMETTVPVTVEMMIVHGRAVVRGT
KRALIVVDLPFGSYEASHTEAFHTAARVLKETGAGAVKLEGGRRMAETVRFLVDRGVPVMGHVGLTPQAINTLGSFKARG
RDDAEASIILDDARAIAEAGAFSIVLEAIAEPLARRITQEVAPPTIGIGGSPACDGQILVLEDMLGLGDRVPKFVKKYAN
LGPDIEKAVASYADEVRARTFPAAEHTYAPRLVEKPAKAS

Sequences:

>Translated_280_residues
MSSQADARRITAPQIQARKGGEPIVSLTSYHSHTARLLDRHVDVILVGDSLGMVMHGMETTVPVTVEMMIVHGRAVVRGT
KRALIVVDLPFGSYEASHTEAFHTAARVLKETGAGAVKLEGGRRMAETVRFLVDRGVPVMGHVGLTPQAINTLGSFKARG
RDDAEASIILDDARAIAEAGAFSIVLEAIAEPLARRITQEVAPPTIGIGGSPACDGQILVLEDMLGLGDRVPKFVKKYAN
LGPDIEKAVASYADEVRARTFPAAEHTYAPRLVEKPAKAS
>Mature_279_residues
SSQADARRITAPQIQARKGGEPIVSLTSYHSHTARLLDRHVDVILVGDSLGMVMHGMETTVPVTVEMMIVHGRAVVRGTK
RALIVVDLPFGSYEASHTEAFHTAARVLKETGAGAVKLEGGRRMAETVRFLVDRGVPVMGHVGLTPQAINTLGSFKARGR
DDAEASIILDDARAIAEAGAFSIVLEAIAEPLARRITQEVAPPTIGIGGSPACDGQILVLEDMLGLGDRVPKFVKKYANL
GPDIEKAVASYADEVRARTFPAAEHTYAPRLVEKPAKAS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=245, Percent_Identity=44.4897959183673, Blast_Score=201, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_XANP2 (A7IF97)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416347.1
- ProteinModelPortal:   A7IF97
- SMR:   A7IF97
- STRING:   A7IF97
- GeneID:   5425495
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1442
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   XAUT78245:XAUT_1442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29874; Mature: 29743

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 88-88 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQADARRITAPQIQARKGGEPIVSLTSYHSHTARLLDRHVDVILVGDSLGMVMHGMET
CCCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCC
TVPVTVEMMIVHGRAVVRGTKRALIVVDLPFGSYEASHTEAFHTAARVLKETGAGAVKLE
CCCEEEEEEEEECHHHEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEC
GGRRMAETVRFLVDRGVPVMGHVGLTPQAINTLGSFKARGRDDAEASIILDDARAIAEAG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHH
AFSIVLEAIAEPLARRITQEVAPPTIGIGGSPACDGQILVLEDMLGLGDRVPKFVKKYAN
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHC
LGPDIEKAVASYADEVRARTFPAAEHTYAPRLVEKPAKAS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SSQADARRITAPQIQARKGGEPIVSLTSYHSHTARLLDRHVDVILVGDSLGMVMHGMET
CCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCC
TVPVTVEMMIVHGRAVVRGTKRALIVVDLPFGSYEASHTEAFHTAARVLKETGAGAVKLE
CCCEEEEEEEEECHHHEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEC
GGRRMAETVRFLVDRGVPVMGHVGLTPQAINTLGSFKARGRDDAEASIILDDARAIAEAG
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHH
AFSIVLEAIAEPLARRITQEVAPPTIGIGGSPACDGQILVLEDMLGLGDRVPKFVKKYAN
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHC
LGPDIEKAVASYADEVRARTFPAAEHTYAPRLVEKPAKAS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA