Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 154245386

GI number: 154245386

Start: 1651249

End: 1652241

Strand: Direct

Name: braE [H]

Synonym: Xaut_1439

Alternate gene names: 154245386

Gene position: 1651249-1652241 (Clockwise)

Preceding gene: 154245385

Following gene: 154245391

Centisome position: 31.1

GC content: 66.06

Gene sequence:

>993_bases
ATGAGCACCTCCACCCCCGCCCATCCCGCCGGTGCGCCCGCGGCCTCCGGCTCCGGCGCCAGCGCCGGCACCATCTTCGG
CATCCTCGCCATCGCGGTGCTGCTGGCGGCGCCGTTCGGCGTCTACCCCGTGTTCCTCATGAAGGTGATGTGCTTCGCGT
TGTTCGCGTGCGCCTTCAACCTGCTTCTGGGCTACACGGGCCTGCTCTCGTTCGGCCACGCCATGTTCTTCGGCGGGTCG
GCCTATGTGTGCGCCCACACCATCAAGGTGATGGGCTTCACCCCGGAGCTGGGCATCCTTGCCGGCGTCGTCTTCGCCGC
CATGCTGGGTTTCATCTCCGGCCTGCTCGCCATCCGCCGCCAGGGCATCTATTTCGCCATGGTGACGCTGGCGCTGGCGC
AGATGTTCTTCTTCTTCTGCCTCCAGGCCCGCTTCACCGGCGGCGAGGACGGCCTTCAGGCGGTGCCGCGCGGGGCGCTG
TTCGGCCTCATCGACCTGTCGAAGGATATCAACCTCTACTACCTGGTGCTGGCCATCTTCGTGTTCGGCTTCCTGGTCAT
CTACCGGGCGATCCACTCGCCGTTCGGTCAGGTGCTGAAGGCCATCCGCGAGAACGAGCCGCGCGCTATCTCTCTGGGCT
ACCGGGTGAACCAGTACAAGCTGCTCGCCTTCGTGCTGTCGGCGGCGCTGGCCGGCCTTGCGGGAGCGACCAAGGTGCTG
GTGTTCCAGCTCGCCTCGCTCACAGACGTGGCCTGGCAGATGTCGGGCGAGGTGATCCTGATGACGCTGGTGGGCGGCAT
GGGCACCATCCTCGGCCCCATCGTTGGCGCGGCGGTGATCGTGACCATGCAGAACTACCTCGCCGGCCTCGGCGAATGGG
TGCTGGTGATCCAGGGCGTCATCTTCGTCCTCGCCGTCTCCCTGTTCCGCAGAGGCTTCGTCGGCGAGGTCATCGCCATC
ATCCAGCAGTTCAAGGCCAAGAAGGCAGGCTGA

Upstream 100 bases:

>100_bases
TACCCGGAGGCCTCCTCCACGGTGATTTTCGTCATCATGGCCATCGTGCTCATGGTGAAGCCCGCCGGCCTGTTCGGCAC
AGCCAAGTGAGCGCGGCGCA

Downstream 100 bases:

>100_bases
GACAGGCCGGCTGAGAAGGCCTGAGACGCACCTCAAATCCCGCGGCTGACCGGTGTCGCCCGCGGGATTTTCGTTTCAGT
GGGCCGCTTCAGGCACGGCG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSTSTPAHPAGAPAASGSGASAGTIFGILAIAVLLAAPFGVYPVFLMKVMCFALFACAFNLLLGYTGLLSFGHAMFFGGS
AYVCAHTIKVMGFTPELGILAGVVFAAMLGFISGLLAIRRQGIYFAMVTLALAQMFFFFCLQARFTGGEDGLQAVPRGAL
FGLIDLSKDINLYYLVLAIFVFGFLVIYRAIHSPFGQVLKAIRENEPRAISLGYRVNQYKLLAFVLSAALAGLAGATKVL
VFQLASLTDVAWQMSGEVILMTLVGGMGTILGPIVGAAVIVTMQNYLAGLGEWVLVIQGVIFVLAVSLFRRGFVGEVIAI
IQQFKAKKAG

Sequences:

>Translated_330_residues
MSTSTPAHPAGAPAASGSGASAGTIFGILAIAVLLAAPFGVYPVFLMKVMCFALFACAFNLLLGYTGLLSFGHAMFFGGS
AYVCAHTIKVMGFTPELGILAGVVFAAMLGFISGLLAIRRQGIYFAMVTLALAQMFFFFCLQARFTGGEDGLQAVPRGAL
FGLIDLSKDINLYYLVLAIFVFGFLVIYRAIHSPFGQVLKAIRENEPRAISLGYRVNQYKLLAFVLSAALAGLAGATKVL
VFQLASLTDVAWQMSGEVILMTLVGGMGTILGPIVGAAVIVTMQNYLAGLGEWVLVIQGVIFVLAVSLFRRGFVGEVIAI
IQQFKAKKAG
>Mature_329_residues
STSTPAHPAGAPAASGSGASAGTIFGILAIAVLLAAPFGVYPVFLMKVMCFALFACAFNLLLGYTGLLSFGHAMFFGGSA
YVCAHTIKVMGFTPELGILAGVVFAAMLGFISGLLAIRRQGIYFAMVTLALAQMFFFFCLQARFTGGEDGLQAVPRGALF
GLIDLSKDINLYYLVLAIFVFGFLVIYRAIHSPFGQVLKAIRENEPRAISLGYRVNQYKLLAFVLSAALAGLAGATKVLV
FQLASLTDVAWQMSGEVILMTLVGGMGTILGPIVGAAVIVTMQNYLAGLGEWVLVIQGVIFVLAVSLFRRGFVGEVIAII
QQFKAKKAG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=281, Percent_Identity=28.8256227758007, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34868; Mature: 34737

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSTPAHPAGAPAASGSGASAGTIFGILAIAVLLAAPFGVYPVFLMKVMCFALFACAFN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLLGYTGLLSFGHAMFFGGSAYVCAHTIKVMGFTPELGILAGVVFAAMLGFISGLLAIRR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QGIYFAMVTLALAQMFFFFCLQARFTGGEDGLQAVPRGALFGLIDLSKDINLYYLVLAIF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
VFGFLVIYRAIHSPFGQVLKAIRENEPRAISLGYRVNQYKLLAFVLSAALAGLAGATKVL
HHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFQLASLTDVAWQMSGEVILMTLVGGMGTILGPIVGAAVIVTMQNYLAGLGEWVLVIQGV
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFVLAVSLFRRGFVGEVIAIIQQFKAKKAG
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSTPAHPAGAPAASGSGASAGTIFGILAIAVLLAAPFGVYPVFLMKVMCFALFACAFN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]