Click here to switch to the map view.

The map label for this gene is cysD [H]

Identifier: 154245346

GI number: 154245346

Start: 1602947

End: 1603741

Strand: Direct

Name: cysD [H]

Synonym: Xaut_1399

Alternate gene names: 154245346

Gene position: 1602947-1603741 (Clockwise)

Preceding gene: 154245335

Following gene: 154245347

Centisome position: 30.19

GC content: 65.91

Gene sequence:

>795_bases
ATGACGCGTCTCGATGCGCTGGAAGCGCAGAGCATCTTCATCTTCCGCGAGGCCTTCGCCCAGCTGCGCAAGATTGCCAT
GCTGTGGTCGCTGGGCAAGGATTCCAACGTGATGATCCACCTGGCGCGCAAGGCCTTCTTCGGCCGCCTGCCATTCCCGG
CCATGCACGTGGATACCCACCGCAAGTTCCCCGAGATGTATGCCTTCCGCGATCACTACGCGAAGGAATGGGACCTCGAC
CTCATCGTCGAGGATTGCCCGCCCATCGAGAGCACCGACCCCACCCTTCCCCCCGCTGCCCGCTCGGCCGCCCGCAAGAC
CGAAGGCCTGCGCCTGGCGCTGGCCAAGCATGGCTTCGACGGTGTCATCGCCGGCATTCGCCGCGACGAGGAGCCGACCC
GGGCCAAGGAGCGCTTCTTCTCGCCGCGCGGCACCGACGGCGCCTGGAACGTGCGCGAGCAGCCGCCGGAATTCTGGGAC
CAGTACAACACTGCCCTGACCCCCGGCGCCCATGTGCGTATCCATCCCATCCTGCATTGGACCGAGATGGACATCTGGGC
CTACACCCAGCGCGAGAACATCCCGGTGATCCCGCTGTATTTCTCCAAGAACGGCAAGCGCTACCGCTCGCTGGGCGACC
AGGACATCACCAACCCGGTGGAGTCGGACGCGTCCACCCTGGACGAGATCCTGGCCGAGCTCGCCATCACCAAGACCTCC
GAGCGCTCGGGCCGCGCCATGGACCACGAATCCGAGGACGCCTTCGAGCGCCTGCGTACCGCCGGCTACCTGTGA

Upstream 100 bases:

>100_bases
TCGGTTTGGATGGGCTCCGGCCCGGCGTGTCCCGCTTTCCGCGGGCAGGCGAAGACCGGGGCAGACCGGCACTTCAGCGG
TGCCACGAAGGAATGACGTC

Downstream 100 bases:

>100_bases
GCCCAGCGCGCCGCATCCCGAGATTTTGAAGAGATCATTGCCATGTCCGCTTCTTCGTTCGCCTCAACGCCTGCCGCGCC
GCTCGCCACCTCGACCGCCG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTRLDALEAQSIFIFREAFAQLRKIAMLWSLGKDSNVMIHLARKAFFGRLPFPAMHVDTHRKFPEMYAFRDHYAKEWDLD
LIVEDCPPIESTDPTLPPAARSAARKTEGLRLALAKHGFDGVIAGIRRDEEPTRAKERFFSPRGTDGAWNVREQPPEFWD
QYNTALTPGAHVRIHPILHWTEMDIWAYTQRENIPVIPLYFSKNGKRYRSLGDQDITNPVESDASTLDEILAELAITKTS
ERSGRAMDHESEDAFERLRTAGYL

Sequences:

>Translated_264_residues
MTRLDALEAQSIFIFREAFAQLRKIAMLWSLGKDSNVMIHLARKAFFGRLPFPAMHVDTHRKFPEMYAFRDHYAKEWDLD
LIVEDCPPIESTDPTLPPAARSAARKTEGLRLALAKHGFDGVIAGIRRDEEPTRAKERFFSPRGTDGAWNVREQPPEFWD
QYNTALTPGAHVRIHPILHWTEMDIWAYTQRENIPVIPLYFSKNGKRYRSLGDQDITNPVESDASTLDEILAELAITKTS
ERSGRAMDHESEDAFERLRTAGYL
>Mature_263_residues
TRLDALEAQSIFIFREAFAQLRKIAMLWSLGKDSNVMIHLARKAFFGRLPFPAMHVDTHRKFPEMYAFRDHYAKEWDLDL
IVEDCPPIESTDPTLPPAARSAARKTEGLRLALAKHGFDGVIAGIRRDEEPTRAKERFFSPRGTDGAWNVREQPPEFWDQ
YNTALTPGAHVRIHPILHWTEMDIWAYTQRENIPVIPLYFSKNGKRYRSLGDQDITNPVESDASTLDEILAELAITKTSE
RSGRAMDHESEDAFERLRTAGYL

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=299, Percent_Identity=41.4715719063545, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 30330; Mature: 30198

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLDALEAQSIFIFREAFAQLRKIAMLWSLGKDSNVMIHLARKAFFGRLPFPAMHVDTH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCEEECCHH
RKFPEMYAFRDHYAKEWDLDLIVEDCPPIESTDPTLPPAARSAARKTEGLRLALAKHGFD
HCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHCCCH
GVIAGIRRDEEPTRAKERFFSPRGTDGAWNVREQPPEFWDQYNTALTPGAHVRIHPILHW
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEE
TEMDIWAYTQRENIPVIPLYFSKNGKRYRSLGDQDITNPVESDASTLDEILAELAITKTS
EECEEEEEECCCCCCEEEEEECCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCC
ERSGRAMDHESEDAFERLRTAGYL
CCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TRLDALEAQSIFIFREAFAQLRKIAMLWSLGKDSNVMIHLARKAFFGRLPFPAMHVDTH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCEEECCHH
RKFPEMYAFRDHYAKEWDLDLIVEDCPPIESTDPTLPPAARSAARKTEGLRLALAKHGFD
HCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHCCCH
GVIAGIRRDEEPTRAKERFFSPRGTDGAWNVREQPPEFWDQYNTALTPGAHVRIHPILHW
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEE
TEMDIWAYTQRENIPVIPLYFSKNGKRYRSLGDQDITNPVESDASTLDEILAELAITKTS
EECEEEEEECCCCCCEEEEEECCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCC
ERSGRAMDHESEDAFERLRTAGYL
CCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA