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The map label for this gene is nirA [H]

Identifier: 154245344

GI number: 154245344

Start: 1600615

End: 1602270

Strand: Reverse

Name: nirA [H]

Synonym: Xaut_1397

Alternate gene names: 154245344

Gene position: 1602270-1600615 (Counterclockwise)

Preceding gene: 154245345

Following gene: 154245343

Centisome position: 30.18

GC content: 66.43

Gene sequence:

>1656_bases
ATGTACGTCTATGACGAGTTCGATCGCACGTTCCTTCAGGAGCGCGTCGCCGAGTTCCGCGACCAGGTGGCCCGCCGCCT
GTCGGGGGAGCTGAACGAGGAGGAGTTCAAGCCGCTCCGCCTCATGAACGGCGTGTACCTGCAGCTCCACGCCTACATGC
TGCGGGTGTGCATTCCCTACGGCACCCTGTCCTCGCAGCAGCTGCGCGCGCTCGGCGCCGTCGCCCGCAAGTACGACCGG
GGCTACGGCCACTTCACCACCCGGCAGAACATCCAGTTCAACTGGATCAAGCTCAACGAGCTGCCGGACGCCATGGCGGA
CCTTGCCAAGGTGGGCATCCACGGCCTCCAGACCTCCGGCAACTGCATCCGCAACACCACCACCGACCCCTGGGCCGGCG
CGGCGGCGGACGAGGTGGAGGACCCGCGCCTCTATGCGGAGGTGATCCGCCAGTGGTCCACCCTGCACCCGGAATTCACC
TACCTGCCGCGCAAGTTCAAGATCGCCATCACCGCGGCCAAGCATGACCGCGCCGCTATCCGCGTGCACGACATCGGCCT
GCGCCTCCACAAGCGCGACGGCGAGACCGGCTTCGAGGTGATGGTGGGCGGCGGGCTCGGGCGTTCGCCCTTCATCGGCA
AAACCATCAAGGACTTCCTGCCGAAGGAGCACCTGCTCTCTTATCTCGAGGCGGTGCTGCGCACCTACAACCTCGGCGGC
CGGCGCGACAACATCTACAAGGCGCGCATCAAGATCCAGGTGCACGAGATGGGCGCGGAGGCCTTCGCCAAGAAGGTGGA
GGCCGAATGGGTCGAGATCCGCGACGGTGCGCTGAAGCTGGACGAGGCCGCCCTCGACGCCATCCGCGACCGCTTCAAGA
TCCCGGACCTCGAGGCGCTGGACGACCAGCCCGCGGAACTCGCCCGCGCGCTCGCCACCGACGCCGCCTTCGCCCGCTGG
CATGCCAATTCCGTCGCCCCCCACAAGGCGAAGGGCCATGCCATCGTCACGCTCTCGCTGAAGCCCGTGGGCGCGCCGCC
CGGCGATGCCACGGCGGAACAGATGGACGCCATCGCCGACCTCGCCGACCAGTATGCCTATGGCGAGATCCGGGTTGCGC
ACGAGCAGAACCTCACCTTCCCCTATGTGCGGCAGAAGGATCTGCCGGCGCTGTGGGCGAAGCTGAAGGCGATCGGCGTC
GCGACCCCCAATGTGGGCCTCGTCACCGACATTATCTCTTGCCCCGGCCTCGACTATTGCTCGCTGGCCAACACCCGCTC
CATCCCCATCGCGCAGAAGCTCACCGAGCGCTTCGCCGACGAGGCACGCATCGCCGACATCGGCCGGCTGCACGTGAACA
TTTCCGGCTGCATCAATGCCTGCGGCCACCACCATGTGGGCCACATCGGCATTCTGGGCGTGGAGAAGAAGGACGAGGAG
TTCTTCCAGATCACCCTTGGCGGCAAGGGCAATTCCAAGGCCCGCATCGGCGAGCTGATCGGCCCCGCCGTCGCGGAAAG
CGAGATCGTGGACGTGGTGGAGAGCCTCGTCGATACCTATCTCAACCTGCGGACCTCACCCGCCGAGACCTTCGTGGCGG
CGGTGGAGCGCCTGGGAATCGAGCCTTTCAAGGAGCGCGCCTATGCCCCTCGTTAA

Upstream 100 bases:

>100_bases
AACCTGCGCGAGCGCATCCGCGTCACCGGCCCCACCATTTCCCTGCCCGGCGCCGCCTGAGATCCCTCAAGGGTCCCCGA
CGCCCAAGCGAGTAGGCCTC

Downstream 100 bases:

>100_bases
GAACGGGGCGCTTGCCGCCGATGCCTTCATCCCCGTCGCCGACGGCGATCCTCTGCCCGACGGGCCGGTGATCGTCTCCG
CCGCGCGCCTGCTGGCGGAC

Product: nitrite/sulfite reductase hemoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MYVYDEFDRTFLQERVAEFRDQVARRLSGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPYGTLSSQQLRALGAVARKYDR
GYGHFTTRQNIQFNWIKLNELPDAMADLAKVGIHGLQTSGNCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHKRDGETGFEVMVGGGLGRSPFIGKTIKDFLPKEHLLSYLEAVLRTYNLGG
RRDNIYKARIKIQVHEMGAEAFAKKVEAEWVEIRDGALKLDEAALDAIRDRFKIPDLEALDDQPAELARALATDAAFARW
HANSVAPHKAKGHAIVTLSLKPVGAPPGDATAEQMDAIADLADQYAYGEIRVAHEQNLTFPYVRQKDLPALWAKLKAIGV
ATPNVGLVTDIISCPGLDYCSLANTRSIPIAQKLTERFADEARIADIGRLHVNISGCINACGHHHVGHIGILGVEKKDEE
FFQITLGGKGNSKARIGELIGPAVAESEIVDVVESLVDTYLNLRTSPAETFVAAVERLGIEPFKERAYAPR

Sequences:

>Translated_551_residues
MYVYDEFDRTFLQERVAEFRDQVARRLSGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPYGTLSSQQLRALGAVARKYDR
GYGHFTTRQNIQFNWIKLNELPDAMADLAKVGIHGLQTSGNCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHKRDGETGFEVMVGGGLGRSPFIGKTIKDFLPKEHLLSYLEAVLRTYNLGG
RRDNIYKARIKIQVHEMGAEAFAKKVEAEWVEIRDGALKLDEAALDAIRDRFKIPDLEALDDQPAELARALATDAAFARW
HANSVAPHKAKGHAIVTLSLKPVGAPPGDATAEQMDAIADLADQYAYGEIRVAHEQNLTFPYVRQKDLPALWAKLKAIGV
ATPNVGLVTDIISCPGLDYCSLANTRSIPIAQKLTERFADEARIADIGRLHVNISGCINACGHHHVGHIGILGVEKKDEE
FFQITLGGKGNSKARIGELIGPAVAESEIVDVVESLVDTYLNLRTSPAETFVAAVERLGIEPFKERAYAPR
>Mature_551_residues
MYVYDEFDRTFLQERVAEFRDQVARRLSGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPYGTLSSQQLRALGAVARKYDR
GYGHFTTRQNIQFNWIKLNELPDAMADLAKVGIHGLQTSGNCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFT
YLPRKFKIAITAAKHDRAAIRVHDIGLRLHKRDGETGFEVMVGGGLGRSPFIGKTIKDFLPKEHLLSYLEAVLRTYNLGG
RRDNIYKARIKIQVHEMGAEAFAKKVEAEWVEIRDGALKLDEAALDAIRDRFKIPDLEALDDQPAELARALATDAAFARW
HANSVAPHKAKGHAIVTLSLKPVGAPPGDATAEQMDAIADLADQYAYGEIRVAHEQNLTFPYVRQKDLPALWAKLKAIGV
ATPNVGLVTDIISCPGLDYCSLANTRSIPIAQKLTERFADEARIADIGRLHVNISGCINACGHHHVGHIGILGVEKKDEE
FFQITLGGKGNSKARIGELIGPAVAESEIVDVVESLVDTYLNLRTSPAETFVAAVERLGIEPFKERAYAPR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=568, Percent_Identity=23.0633802816901, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 61279; Mature: 61279

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVYDEFDRTFLQERVAEFRDQVARRLSGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GTLSSQQLRALGAVARKYDRGYGHFTTRQNIQFNWIKLNELPDAMADLAKVGIHGLQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
NCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFTYLPRKFKIAITAAKHDRAAI
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCEEE
RVHDIGLRLHKRDGETGFEVMVGGGLGRSPFIGKTIKDFLPKEHLLSYLEAVLRTYNLGG
EEEEEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCC
RRDNIYKARIKIQVHEMGAEAFAKKVEAEWVEIRDGALKLDEAALDAIRDRFKIPDLEAL
CCCCEEEEEEEEEEECCCHHHHHHHHHHHHHEECCCCEEHHHHHHHHHHHHCCCCCCHHC
DDQPAELARALATDAAFARWHANSVAPHKAKGHAIVTLSLKPVGAPPGDATAEQMDAIAD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
LADQYAYGEIRVAHEQNLTFPYVRQKDLPALWAKLKAIGVATPNVGLVTDIISCPGLDYC
HHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
SLANTRSIPIAQKLTERFADEARIADIGRLHVNISGCINACGHHHVGHIGILGVEKKDEE
HCCCCCCCCHHHHHHHHHHHHHHHEECEEEEEEHHHHHHHCCCCCCCEEEEEECCCCCCE
FFQITLGGKGNSKARIGELIGPAVAESEIVDVVESLVDTYLNLRTSPAETFVAAVERLGI
EEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
EPFKERAYAPR
CHHHHCCCCCC
>Mature Secondary Structure
MYVYDEFDRTFLQERVAEFRDQVARRLSGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GTLSSQQLRALGAVARKYDRGYGHFTTRQNIQFNWIKLNELPDAMADLAKVGIHGLQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
NCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFTYLPRKFKIAITAAKHDRAAI
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCEEE
RVHDIGLRLHKRDGETGFEVMVGGGLGRSPFIGKTIKDFLPKEHLLSYLEAVLRTYNLGG
EEEEEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCC
RRDNIYKARIKIQVHEMGAEAFAKKVEAEWVEIRDGALKLDEAALDAIRDRFKIPDLEAL
CCCCEEEEEEEEEEECCCHHHHHHHHHHHHHEECCCCEEHHHHHHHHHHHHCCCCCCHHC
DDQPAELARALATDAAFARWHANSVAPHKAKGHAIVTLSLKPVGAPPGDATAEQMDAIAD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
LADQYAYGEIRVAHEQNLTFPYVRQKDLPALWAKLKAIGVATPNVGLVTDIISCPGLDYC
HHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
SLANTRSIPIAQKLTERFADEARIADIGRLHVNISGCINACGHHHVGHIGILGVEKKDEE
HCCCCCCCCHHHHHHHHHHHHHHHEECEEEEEEHHHHHHHCCCCCCCEEEEEECCCCCCE
FFQITLGGKGNSKARIGELIGPAVAESEIVDVVESLVDTYLNLRTSPAETFVAAVERLGI
EEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
EPFKERAYAPR
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]