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The map label for this gene is fumB [H]

Identifier: 154245307

GI number: 154245307

Start: 1561987

End: 1563555

Strand: Direct

Name: fumB [H]

Synonym: Xaut_1360

Alternate gene names: 154245307

Gene position: 1561987-1563555 (Clockwise)

Preceding gene: 154245306

Following gene: 154245308

Centisome position: 29.42

GC content: 69.73

Gene sequence:

>1569_bases
ATGTGTTCCGCCCAAACAAGTTCCGCCCAAGCAGGTTCCGCCCAAATCCTCCGCACCGGCCCTGCAAAAGTCATCCGCGA
GGACGACATCATCCAGAGCGTCGCCGACGCGCTCCAGTACATCTCCTTCTTTCATCCGGCGGACTACATCGCCCATCTCG
CCCGCGCCCATGCGCGCGAAGCCTCCCCCGCGGCGCGCGATGCCATCGCCCAGATCCTCGTGAACTCGCGCATGGCGGCG
CTGGGGCGACGGCCCATCTGCCAGGATACCGGCCTCGTGGTGGTGTTCGCCAAGGTGGGGATGGATGTGCGCATCGACAG
CGCCCGCCCCTTCGCCGACCTCATCAACGATGCCGTGCGCCGCGCTTATCTGGACGAGGCCAATCCCCTGCGCGCCTCCA
TGGTGGGGGACCCGCTGTTCGAGCGGCGCAACACCAGGGACAACGCCCCGGCGGTGGTGCATGTGGAACTGGTGCCGGGC
GCCACGCTGGAGATCACGGTGGCGGCCAAGGGCGGCGGCTCGGAGAACAAGGCGAAGTTCGTGACGCTGGATCCCGGCGC
CAGCGTCGAGGACTGGGTGCTGAAGACCGTGCCGGAGCTGGGCGCCGGCTGGTGCCCGCCCGGTGTCATCGGCCTCGGCA
TCGGCGGCTCGGCGGAAAAGGCCATGCTGATCGCCAAGGAGGCGCTCTTGGAGGAGATCGACATGACCGACCTCCTCGCC
CGCGGCCCCTCCAGCAAATTGGAGGAGATGCGCATCTCCCTCTACGAGAAGATCAACGCGCTGGGCATCGGCGCGCAGGG
CCTCGGCGGCCTGACTACAGTGGTGGACGTGAAGATCGCCACCTTCCCGACCCATGCGGCGTCGAAGCCCGTGGCGCTCA
TTCCCCAGTGCGCCGCCAACCGCCATGCCACCATCGTGCTCGATGGCTCGGGGCCGGTGCGGCTCGATCCGCCGGACCTC
TCGGCCTGGCCGCAGATGGTGCTGGATGCCGCCGACGGAAGCGCCCGCCGGGTGAACCTCGATGGGCTGACGCGGGAGGA
GGTCGGCTCCTGGACGCCGGGGCAGAAGCTGCTGCTGACCGGCCGCATGCTCACCGGCCGCGACGCCGCCCATCAGCGCC
TCACCGACATGCTGAAGGCCGGCACGCCCCTGCCGGTGAACCTCGACGGGCGCGTCATCTATTATGTCGGCCCGGTGGAC
CCGGTGGGGGACGAGGCGGTGGGCCCGGCCGGCCCCACCACCTCCACCCGCATGGATCGCTACACCGATGCCATCCTCGG
CACCGGCCTGCTCGCCATGGTGGGCAAGGCGGAGCGGGGGCCGGCGGCGATCGCGGCCATCGCCCGGCACAGGGCGGCCT
ATCTCGTCGCCGTGGGCGGGGCGGCCTATCTCGTCTCCAAGGCCATCAAGGCGGCGCGGGTGGTGGCCTTCGAAGACCTC
GGCATGGAGGCCATCTACGAGTTCGAGGTGGAGGACATGCCGGTGACGGTGGCAGTGAGCGCCGCGGGCCAGTCCATCCA
CACCCTTGGACCGGCACTCTGGCGGGAGCGCATCGCCGCGCAGCCCTGA

Upstream 100 bases:

>100_bases
GTTCCGCCGCGGTCGAGCACGGCGGCTGACGCTCTGAGATCGGATGGGGAGGCGGCAGCCAGTCGCTTCCCCATCCCCAC
GGCAAGGAAAGCCAGGCGCC

Downstream 100 bases:

>100_bases
GCAGGAGCCGCCTTCGTGGCCTGTGAAGGCGATCTTCGGGAACGATTGACCCGGCGCAACGTTTTTCCTCGCAGTTGCAG
TCAGGCTTTCGCGCCGTGGC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MCSAQTSSAQAGSAQILRTGPAKVIREDDIIQSVADALQYISFFHPADYIAHLARAHAREASPAARDAIAQILVNSRMAA
LGRRPICQDTGLVVVFAKVGMDVRIDSARPFADLINDAVRRAYLDEANPLRASMVGDPLFERRNTRDNAPAVVHVELVPG
ATLEITVAAKGGGSENKAKFVTLDPGASVEDWVLKTVPELGAGWCPPGVIGLGIGGSAEKAMLIAKEALLEEIDMTDLLA
RGPSSKLEEMRISLYEKINALGIGAQGLGGLTTVVDVKIATFPTHAASKPVALIPQCAANRHATIVLDGSGPVRLDPPDL
SAWPQMVLDAADGSARRVNLDGLTREEVGSWTPGQKLLLTGRMLTGRDAAHQRLTDMLKAGTPLPVNLDGRVIYYVGPVD
PVGDEAVGPAGPTTSTRMDRYTDAILGTGLLAMVGKAERGPAAIAAIARHRAAYLVAVGGAAYLVSKAIKAARVVAFEDL
GMEAIYEFEVEDMPVTVAVSAAGQSIHTLGPALWRERIAAQP

Sequences:

>Translated_522_residues
MCSAQTSSAQAGSAQILRTGPAKVIREDDIIQSVADALQYISFFHPADYIAHLARAHAREASPAARDAIAQILVNSRMAA
LGRRPICQDTGLVVVFAKVGMDVRIDSARPFADLINDAVRRAYLDEANPLRASMVGDPLFERRNTRDNAPAVVHVELVPG
ATLEITVAAKGGGSENKAKFVTLDPGASVEDWVLKTVPELGAGWCPPGVIGLGIGGSAEKAMLIAKEALLEEIDMTDLLA
RGPSSKLEEMRISLYEKINALGIGAQGLGGLTTVVDVKIATFPTHAASKPVALIPQCAANRHATIVLDGSGPVRLDPPDL
SAWPQMVLDAADGSARRVNLDGLTREEVGSWTPGQKLLLTGRMLTGRDAAHQRLTDMLKAGTPLPVNLDGRVIYYVGPVD
PVGDEAVGPAGPTTSTRMDRYTDAILGTGLLAMVGKAERGPAAIAAIARHRAAYLVAVGGAAYLVSKAIKAARVVAFEDL
GMEAIYEFEVEDMPVTVAVSAAGQSIHTLGPALWRERIAAQP
>Mature_522_residues
MCSAQTSSAQAGSAQILRTGPAKVIREDDIIQSVADALQYISFFHPADYIAHLARAHAREASPAARDAIAQILVNSRMAA
LGRRPICQDTGLVVVFAKVGMDVRIDSARPFADLINDAVRRAYLDEANPLRASMVGDPLFERRNTRDNAPAVVHVELVPG
ATLEITVAAKGGGSENKAKFVTLDPGASVEDWVLKTVPELGAGWCPPGVIGLGIGGSAEKAMLIAKEALLEEIDMTDLLA
RGPSSKLEEMRISLYEKINALGIGAQGLGGLTTVVDVKIATFPTHAASKPVALIPQCAANRHATIVLDGSGPVRLDPPDL
SAWPQMVLDAADGSARRVNLDGLTREEVGSWTPGQKLLLTGRMLTGRDAAHQRLTDMLKAGTPLPVNLDGRVIYYVGPVD
PVGDEAVGPAGPTTSTRMDRYTDAILGTGLLAMVGKAERGPAAIAAIARHRAAYLVAVGGAAYLVSKAIKAARVVAFEDL
GMEAIYEFEVEDMPVTVAVSAAGQSIHTLGPALWRERIAAQP

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=485, Percent_Identity=31.7525773195876, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787897, Length=489, Percent_Identity=31.9018404907975, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789442, Length=287, Percent_Identity=29.2682926829268, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789443, Length=169, Percent_Identity=30.1775147928994, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54979; Mature: 54979

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSAQTSSAQAGSAQILRTGPAKVIREDDIIQSVADALQYISFFHPADYIAHLARAHARE
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
ASPAARDAIAQILVNSRMAALGRRPICQDTGLVVVFAKVGMDVRIDSARPFADLINDAVR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHH
RAYLDEANPLRASMVGDPLFERRNTRDNAPAVVHVELVPGATLEITVAAKGGGSENKAKF
HHHHCCCCCCHHHHCCCHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEE
VTLDPGASVEDWVLKTVPELGAGWCPPGVIGLGIGGSAEKAMLIAKEALLEEIDMTDLLA
EEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
RGPSSKLEEMRISLYEKINALGIGAQGLGGLTTVVDVKIATFPTHAASKPVALIPQCAAN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCHHCCC
RHATIVLDGSGPVRLDPPDLSAWPQMVLDAADGSARRVNLDGLTREEVGSWTPGQKLLLT
CCEEEEEECCCCEEECCCCCCHHHHHHEECCCCCCEEEECCCCCHHHHCCCCCCCEEEEE
GRMLTGRDAAHQRLTDMLKAGTPLPVNLDGRVIYYVGPVDPVGDEAVGPAGPTTSTRMDR
CCEECCCHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
YTDAILGTGLLAMVGKAERGPAAIAAIARHRAAYLVAVGGAAYLVSKAIKAARVVAFEDL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHC
GMEAIYEFEVEDMPVTVAVSAAGQSIHTLGPALWRERIAAQP
CCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCSAQTSSAQAGSAQILRTGPAKVIREDDIIQSVADALQYISFFHPADYIAHLARAHARE
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
ASPAARDAIAQILVNSRMAALGRRPICQDTGLVVVFAKVGMDVRIDSARPFADLINDAVR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHH
RAYLDEANPLRASMVGDPLFERRNTRDNAPAVVHVELVPGATLEITVAAKGGGSENKAKF
HHHHCCCCCCHHHHCCCHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCEEE
VTLDPGASVEDWVLKTVPELGAGWCPPGVIGLGIGGSAEKAMLIAKEALLEEIDMTDLLA
EEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
RGPSSKLEEMRISLYEKINALGIGAQGLGGLTTVVDVKIATFPTHAASKPVALIPQCAAN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCHHCCC
RHATIVLDGSGPVRLDPPDLSAWPQMVLDAADGSARRVNLDGLTREEVGSWTPGQKLLLT
CCEEEEEECCCCEEECCCCCCHHHHHHEECCCCCCEEEECCCCCHHHHCCCCCCCEEEEE
GRMLTGRDAAHQRLTDMLKAGTPLPVNLDGRVIYYVGPVDPVGDEAVGPAGPTTSTRMDR
CCEECCCHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
YTDAILGTGLLAMVGKAERGPAAIAAIARHRAAYLVAVGGAAYLVSKAIKAARVVAFEDL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHC
GMEAIYEFEVEDMPVTVAVSAAGQSIHTLGPALWRERIAAQP
CCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]