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The map label for this gene is citE [C]

Identifier: 154245295

GI number: 154245295

Start: 1549506

End: 1550336

Strand: Reverse

Name: citE [C]

Synonym: Xaut_1348

Alternate gene names: 154245295

Gene position: 1550336-1549506 (Counterclockwise)

Preceding gene: 154245309

Following gene: 154245294

Centisome position: 29.2

GC content: 73.04

Gene sequence:

>831_bases
ATGTCCGAGTTTGCCCGGCCCGTTTCGCTCAGGAGCGGGCGGCTCGTTGCCCTGTTTGTCCCGGCGACGCGTCCCGATCG
CTTCGCCAAAGCGCTCGCCTCCGGCGCCGACGCCATCATCATCGACCTCGAGGATGCGGTGGGCCACGCAGAGAAGGATG
CCGCCCGCGATGCCCTCGCCGCCGCCATCCCGGCCGGAGGACCGGTGCTGCTGCGAATCAACGCCTCTGGCACCCCGTGG
CACGCGGAGGATCTCGCCGTCGCGCGGCGGCTGCCCCTTGCCGGCGTCATGCTGCCGAAGGCCGAGAGCCGGGACGAGGT
GGAGCAGGTGGCCGCCCGCCTCGGCGAGGGCGTTGCCCTCATTCCCCTCATCGAAAGCGCCCGGGGCCTTGCCGCGGCGC
GGTCCATCGCGGCGGTGCCGTGCGTAGCCCGCCTCGCCTTCGGCTCCATCGATTTCGCCGCTGATCTGGGCTGTGCCCAT
ACCCGCGAGGCGCTCCTTTCCGCCCGTTCCGAGCTGGTGCTGGCGGCGCGGCTCGCCGGCGGTCAGGGCCCCATGGACGG
GGTCACCACCCGCGTCGACAACCCCGCCCTCGCGCAGGACGACGCGGCCCATGCGGTGGCCCTCGGCTTCGCCGGCAAGC
TGTGCATCCACCCCGGCCAGATCGCCCCCGTGATGACAGGCTTTGCGCCGACCGCCGAAGAGGTGAGCTGGGCCGAGCGC
ATCCTCGCCGCCCTAGACGCGGGCGGTTCGGTCGTGACGGTGGACGGCGCCATGGTGGACGCCCCCGTGCGCCTGCGCGC
CGAGCGCATCCTAGCCCGCACAGGCCGCTGA

Upstream 100 bases:

>100_bases
CTCCCGCCGCGTTTGCGGTGACTGTGCCCCCGTTTCCATGACGTCGCGTCGCGCCGGTCCTTGCCGAGCGCGCGCCGACC
CCATCCGACGGGTTTCGACC

Downstream 100 bases:

>100_bases
AAGGTCAAGCCATGAGCAATGAGACCCTCACCCTCGCCGCCTATGCCGCCGGCATCCGGCTCGCGGACATCCCCGCCGAT
GTGGTCGCGCGGGCGGAGCG

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSEFARPVSLRSGRLVALFVPATRPDRFAKALASGADAIIIDLEDAVGHAEKDAARDALAAAIPAGGPVLLRINASGTPW
HAEDLAVARRLPLAGVMLPKAESRDEVEQVAARLGEGVALIPLIESARGLAAARSIAAVPCVARLAFGSIDFAADLGCAH
TREALLSARSELVLAARLAGGQGPMDGVTTRVDNPALAQDDAAHAVALGFAGKLCIHPGQIAPVMTGFAPTAEEVSWAER
ILAALDAGGSVVTVDGAMVDAPVRLRAERILARTGR

Sequences:

>Translated_276_residues
MSEFARPVSLRSGRLVALFVPATRPDRFAKALASGADAIIIDLEDAVGHAEKDAARDALAAAIPAGGPVLLRINASGTPW
HAEDLAVARRLPLAGVMLPKAESRDEVEQVAARLGEGVALIPLIESARGLAAARSIAAVPCVARLAFGSIDFAADLGCAH
TREALLSARSELVLAARLAGGQGPMDGVTTRVDNPALAQDDAAHAVALGFAGKLCIHPGQIAPVMTGFAPTAEEVSWAER
ILAALDAGGSVVTVDGAMVDAPVRLRAERILARTGR
>Mature_275_residues
SEFARPVSLRSGRLVALFVPATRPDRFAKALASGADAIIIDLEDAVGHAEKDAARDALAAAIPAGGPVLLRINASGTPWH
AEDLAVARRLPLAGVMLPKAESRDEVEQVAARLGEGVALIPLIESARGLAAARSIAAVPCVARLAFGSIDFAADLGCAHT
REALLSARSELVLAARLAGGQGPMDGVTTRVDNPALAQDDAAHAVALGFAGKLCIHPGQIAPVMTGFAPTAEEVSWAERI
LAALDAGGSVVTVDGAMVDAPVRLRAERILARTGR

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=290, Percent_Identity=29.6551724137931, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI87081764, Length=281, Percent_Identity=33.8078291814947, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557458, Length=315, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28343; Mature: 28212

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFARPVSLRSGRLVALFVPATRPDRFAKALASGADAIIIDLEDAVGHAEKDAARDALA
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHH
AAIPAGGPVLLRINASGTPWHAEDLAVARRLPLAGVMLPKAESRDEVEQVAARLGEGVAL
HHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCEEE
IPLIESARGLAAARSIAAVPCVARLAFGSIDFAADLGCAHTREALLSARSELVLAARLAG
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHEEEEECCC
GQGPMDGVTTRVDNPALAQDDAAHAVALGFAGKLCIHPGQIAPVMTGFAPTAEEVSWAER
CCCCCCCCCCCCCCCCCCCCCCHHEEECCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHH
ILAALDAGGSVVTVDGAMVDAPVRLRAERILARTGR
HHHHHCCCCCEEEECCEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEFARPVSLRSGRLVALFVPATRPDRFAKALASGADAIIIDLEDAVGHAEKDAARDALA
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHCCCHHHHHHHHHH
AAIPAGGPVLLRINASGTPWHAEDLAVARRLPLAGVMLPKAESRDEVEQVAARLGEGVAL
HHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCEEE
IPLIESARGLAAARSIAAVPCVARLAFGSIDFAADLGCAHTREALLSARSELVLAARLAG
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHEEEEECCC
GQGPMDGVTTRVDNPALAQDDAAHAVALGFAGKLCIHPGQIAPVMTGFAPTAEEVSWAER
CCCCCCCCCCCCCCCCCCCCCCHHEEECCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHH
ILAALDAGGSVVTVDGAMVDAPVRLRAERILARTGR
HHHHHCCCCCEEEECCEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]