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The map label for this gene is hyuA [H]

Identifier: 154245286

GI number: 154245286

Start: 1537131

End: 1539062

Strand: Direct

Name: hyuA [H]

Synonym: Xaut_1338

Alternate gene names: 154245286

Gene position: 1537131-1539062 (Clockwise)

Preceding gene: 154245285

Following gene: 154245287

Centisome position: 28.95

GC content: 67.7

Gene sequence:

>1932_bases
ATGTCCCTTCAGATCAACATCGACAACGGCGGCACGCTGACGGACATCTGTATTCTGTCAGATGATGCGGTGAGAAAGAC
CAAGGTCCTCACCACGCCTTATGATCTCAGCAAGTGCTTCTTCGAGGGCCTGACCAAGGCTTCCGGCGTGCTCTACGGCG
CGCCGGATGTGAAGCGTCTGCTGGAGGAGGTGGACCTCATCCGCTATTCCACCACCCAGGGCACCAACGCGATCTGCGAG
CGCAAGGGGCCCCGGCTCGGCCTCATCCTCGATGCATCCGCGCACGACCTGCCGAGCCGCCTCGCGGCCCACGACCAGGA
TGTGTTCGAGGCCCTGGTGGGCGACCGGATCGCCTTCCTCGATGCCGCCGCCATCAGCGCCGACGAGGCGGACGTGGCGG
TGGCGAAGGCCATCAACACCCTCACCGCCGCCGGTGCCAACCGCCTGGTGGTGAGCTTCGGCGGCGCCGATTTCCAGGCG
CTGGAGAACGACTTCCGCCTTGTCGCCCTGCGCAAGTATCCGCGCCACCTGCTCGGCGCCGTGCCGATCCTCTACGGCTC
CGATCTCACCACCGACCCGGATGGCGAGCGGCGCACCTGGACCGCGCTCATCAACTCGTTCCTCCACCCCTCCATGGAGG
CGTTCCTCTACAATGCGGAGAACCGGCTGCGGGCCTATCGCACCAAGAACCCGCTGCTCATCTTCCGCAATGACGGCGAC
GCCTCGCGCGTGGCCAAGACCATCGCCATCAAGACCTATTCGTCCGGCCCGCGCGGCGGCATGGAGGGCGTGAAGTCGTT
CTCGCGCCGCTACGGCTTCGCCGACACCGTTTCCATCGACGTGGGCGGCACCACCACGGATATCGGCCAGTACATCGACG
GCACCGTGGCCGAGGTGCGGCGCGGTCATGTGGAAGGCATCTCCGTCTCCTTCCCCCTGTGCGAAATCCTGAGCGCGGGG
GCGGGCGGCTCCTCCATCTTCAAGGTGGTGGACGGGCGCATCGTCATCGGGCCGGAGAGCGTCGGCGCGGTTCCGGGGCC
GGCCTGCTTCGGGCGCGGCGGGCGCGAGGCCACCATCACCGACGCCAGCCTGCTCATCGGCCTGTTCGATCCCACCTCCT
ATTTCGGCGGCGGCATGGGGCTCGACGCGGACCGCGCGGCGGCGGCGGTGACCGCCAGCGTCGCCAGCCGGCTGGGGCTC
GGGCTCGATGATGCGCTGCTGCGCATGGAATTCGCCTATGAGGAGAAGATCGCGGCGGAGCTTCACCGCTTCACCAAGAT
CTCCGACGAGACGGTGATGCTCGCCTTCGGCGGCGCAGGCCCCCTCAACGCCTGCGGCGTCGCCGAGAAGGCGGGCATTC
GCCGGGTGGCGATCCCGCAGATGGCGGCGGTGTTCTCGGCCTACGGCATCGGCTCCTGCGATGTCTCCCAGCGCTATGCG
GTGACGCTGAGCGATGCCGCCGAGCTTTCCGGCACCATCGCGGCGCTGAAGGTGAAGGCGTCCCGCGACATGTTCGCGGA
AGGCTGCGCCGACGGCACCTACACCCTCGATGCACGCCTCGTCGCCGACTTCGGGGACGGGCGCACGCTGGTCCACGTGC
TGGGCGACCAGCTGGAGGTTCCGGCCGCCTTCGCCGGGGCTCGCTCGGTGGAAGTGGAACTGAAGGCGGTCCGCAGTCTG
CGCGAAGGCGCGGAGCGGTCGGCTCGCTTCGCGCCCCGCTCGTCGGCCGCAGCCCATGGCAAGCGCAACGTGCTGATCCG
TGATCGCGGGCGCATCGACGTGCCGGTCTACCGCCTCGCCGATCTTGCAGATGGCGACTTCGCATCCGGCCCCGCCATCC
TTGAGGAAGATTATTTCACCTGCCGGGTCCCTGAAAACTGGCAGTTCGTCATCAGCGATGCAGGCGACATCCTGCTGAGC
CGGGAGGATTGA

Upstream 100 bases:

>100_bases
GGACCGTGTGTCGACCTGGCCGCGAGCGCCGCTGCGGCGCGCCCGGGCCTGCCGACGCATGAAGCCGCAGCCGGAGAAGA
CGTGGGAGGAGAAAGAGCGT

Downstream 100 bases:

>100_bases
AGAACATGAAAGTCGCCATGACCGAATATCTCCGCATCAACCTGAAGTCGGAGAGGTGGGAATGCCGCGTGTGCGATCAC
GACATTGCGCCGGCACGGGG

Product: hydantoinase/oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSLQINIDNGGTLTDICILSDDAVRKTKVLTTPYDLSKCFFEGLTKASGVLYGAPDVKRLLEEVDLIRYSTTQGTNAICE
RKGPRLGLILDASAHDLPSRLAAHDQDVFEALVGDRIAFLDAAAISADEADVAVAKAINTLTAAGANRLVVSFGGADFQA
LENDFRLVALRKYPRHLLGAVPILYGSDLTTDPDGERRTWTALINSFLHPSMEAFLYNAENRLRAYRTKNPLLIFRNDGD
ASRVAKTIAIKTYSSGPRGGMEGVKSFSRRYGFADTVSIDVGGTTTDIGQYIDGTVAEVRRGHVEGISVSFPLCEILSAG
AGGSSIFKVVDGRIVIGPESVGAVPGPACFGRGGREATITDASLLIGLFDPTSYFGGGMGLDADRAAAAVTASVASRLGL
GLDDALLRMEFAYEEKIAAELHRFTKISDETVMLAFGGAGPLNACGVAEKAGIRRVAIPQMAAVFSAYGIGSCDVSQRYA
VTLSDAAELSGTIAALKVKASRDMFAEGCADGTYTLDARLVADFGDGRTLVHVLGDQLEVPAAFAGARSVEVELKAVRSL
REGAERSARFAPRSSAAAHGKRNVLIRDRGRIDVPVYRLADLADGDFASGPAILEEDYFTCRVPENWQFVISDAGDILLS
RED

Sequences:

>Translated_643_residues
MSLQINIDNGGTLTDICILSDDAVRKTKVLTTPYDLSKCFFEGLTKASGVLYGAPDVKRLLEEVDLIRYSTTQGTNAICE
RKGPRLGLILDASAHDLPSRLAAHDQDVFEALVGDRIAFLDAAAISADEADVAVAKAINTLTAAGANRLVVSFGGADFQA
LENDFRLVALRKYPRHLLGAVPILYGSDLTTDPDGERRTWTALINSFLHPSMEAFLYNAENRLRAYRTKNPLLIFRNDGD
ASRVAKTIAIKTYSSGPRGGMEGVKSFSRRYGFADTVSIDVGGTTTDIGQYIDGTVAEVRRGHVEGISVSFPLCEILSAG
AGGSSIFKVVDGRIVIGPESVGAVPGPACFGRGGREATITDASLLIGLFDPTSYFGGGMGLDADRAAAAVTASVASRLGL
GLDDALLRMEFAYEEKIAAELHRFTKISDETVMLAFGGAGPLNACGVAEKAGIRRVAIPQMAAVFSAYGIGSCDVSQRYA
VTLSDAAELSGTIAALKVKASRDMFAEGCADGTYTLDARLVADFGDGRTLVHVLGDQLEVPAAFAGARSVEVELKAVRSL
REGAERSARFAPRSSAAAHGKRNVLIRDRGRIDVPVYRLADLADGDFASGPAILEEDYFTCRVPENWQFVISDAGDILLS
RED
>Mature_642_residues
SLQINIDNGGTLTDICILSDDAVRKTKVLTTPYDLSKCFFEGLTKASGVLYGAPDVKRLLEEVDLIRYSTTQGTNAICER
KGPRLGLILDASAHDLPSRLAAHDQDVFEALVGDRIAFLDAAAISADEADVAVAKAINTLTAAGANRLVVSFGGADFQAL
ENDFRLVALRKYPRHLLGAVPILYGSDLTTDPDGERRTWTALINSFLHPSMEAFLYNAENRLRAYRTKNPLLIFRNDGDA
SRVAKTIAIKTYSSGPRGGMEGVKSFSRRYGFADTVSIDVGGTTTDIGQYIDGTVAEVRRGHVEGISVSFPLCEILSAGA
GGSSIFKVVDGRIVIGPESVGAVPGPACFGRGGREATITDASLLIGLFDPTSYFGGGMGLDADRAAAAVTASVASRLGLG
LDDALLRMEFAYEEKIAAELHRFTKISDETVMLAFGGAGPLNACGVAEKAGIRRVAIPQMAAVFSAYGIGSCDVSQRYAV
TLSDAAELSGTIAALKVKASRDMFAEGCADGTYTLDARLVADFGDGRTLVHVLGDQLEVPAAFAGARSVEVELKAVRSLR
EGAERSARFAPRSSAAAHGKRNVLIRDRGRIDVPVYRLADLADGDFASGPAILEEDYFTCRVPENWQFVISDAGDILLSR
ED

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=522, Percent_Identity=26.2452107279693, Blast_Score=103, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI133901900, Length=398, Percent_Identity=26.1306532663317, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322634, Length=735, Percent_Identity=23.1292517006803, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45550492, Length=580, Percent_Identity=25.1724137931034, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 68391; Mature: 68260

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQINIDNGGTLTDICILSDDAVRKTKVLTTPYDLSKCFFEGLTKASGVLYGAPDVKRL
CEEEEEECCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEECCHHHHHH
LEEVDLIRYSTTQGTNAICERKGPRLGLILDASAHDLPSRLAAHDQDVFEALVGDRIAFL
HHHHHHHEEECCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHCHHHHHHHHHCCCEEEE
DAAAISADEADVAVAKAINTLTAAGANRLVVSFGGADFQALENDFRLVALRKYPRHLLGA
EHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCEEEEEHHHHHHHHHC
VPILYGSDLTTDPDGERRTWTALINSFLHPSMEAFLYNAENRLRAYRTKNPLLIFRNDGD
CCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEECCHHHHHHEECCCCEEEEECCCC
ASRVAKTIAIKTYSSGPRGGMEGVKSFSRRYGFADTVSIDVGGTTTDIGQYIDGTVAEVR
HHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHH
RGHVEGISVSFPLCEILSAGAGGSSIFKVVDGRIVIGPESVGAVPGPACFGRGGREATIT
CCCCCCEEECCCHHHHHHCCCCCCHHHEEECCEEEECCCCCCCCCCCHHHCCCCCEEEEE
DASLLIGLFDPTSYFGGGMGLDADRAAAAVTASVASRLGLGLDDALLRMEFAYEEKIAAE
CCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LHRFTKISDETVMLAFGGAGPLNACGVAEKAGIRRVAIPQMAAVFSAYGIGSCDVSQRYA
HHHHHCCCCCEEEEEECCCCCCCCCCCHHHCCCEEECHHHHHHHHHHCCCCCCCCCCEEE
VTLSDAAELSGTIAALKVKASRDMFAEGCADGTYTLDARLVADFGDGRTLVHVLGDQLEV
EEECCHHHHCCCEEEEEEECCHHHHHHCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCC
PAAFAGARSVEVELKAVRSLREGAERSARFAPRSSAAAHGKRNVLIRDRGRIDVPVYRLA
CHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHH
DLADGDFASGPAILEEDYFTCRVPENWQFVISDAGDILLSRED
HCCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCEEEECCC
>Mature Secondary Structure 
SLQINIDNGGTLTDICILSDDAVRKTKVLTTPYDLSKCFFEGLTKASGVLYGAPDVKRL
EEEEEECCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCEEECCHHHHHH
LEEVDLIRYSTTQGTNAICERKGPRLGLILDASAHDLPSRLAAHDQDVFEALVGDRIAFL
HHHHHHHEEECCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHCHHHHHHHHHCCCEEEE
DAAAISADEADVAVAKAINTLTAAGANRLVVSFGGADFQALENDFRLVALRKYPRHLLGA
EHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCEEEEEHHHHHHHHHC
VPILYGSDLTTDPDGERRTWTALINSFLHPSMEAFLYNAENRLRAYRTKNPLLIFRNDGD
CCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEECCHHHHHHEECCCCEEEEECCCC
ASRVAKTIAIKTYSSGPRGGMEGVKSFSRRYGFADTVSIDVGGTTTDIGQYIDGTVAEVR
HHHHEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHH
RGHVEGISVSFPLCEILSAGAGGSSIFKVVDGRIVIGPESVGAVPGPACFGRGGREATIT
CCCCCCEEECCCHHHHHHCCCCCCHHHEEECCEEEECCCCCCCCCCCHHHCCCCCEEEEE
DASLLIGLFDPTSYFGGGMGLDADRAAAAVTASVASRLGLGLDDALLRMEFAYEEKIAAE
CCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LHRFTKISDETVMLAFGGAGPLNACGVAEKAGIRRVAIPQMAAVFSAYGIGSCDVSQRYA
HHHHHCCCCCEEEEEECCCCCCCCCCCHHHCCCEEECHHHHHHHHHHCCCCCCCCCCEEE
VTLSDAAELSGTIAALKVKASRDMFAEGCADGTYTLDARLVADFGDGRTLVHVLGDQLEV
EEECCHHHHCCCEEEEEEECCHHHHHHCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCC
PAAFAGARSVEVELKAVRSLREGAERSARFAPRSSAAAHGKRNVLIRDRGRIDVPVYRLA
CHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHH
DLADGDFASGPAILEEDYFTCRVPENWQFVISDAGDILLSRED
HCCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]