Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 154245275

GI number: 154245275

Start: 1522610

End: 1523704

Strand: Direct

Name: nemA [H]

Synonym: Xaut_1327

Alternate gene names: 154245275

Gene position: 1522610-1523704 (Clockwise)

Preceding gene: 154245274

Following gene: 154245276

Centisome position: 28.68

GC content: 69.04

Gene sequence:

>1095_bases
ATGAGCGCGCCCACCCTGTTCGACAGCTTCCGCCTCGGCGACATGACCCTGCCCAACCGCATCGTCATGGCGCCGCTGAC
CCGTAACCGCGCCGGTGCCGGCTTCGTGCCGAGCCCGCTGGCTCCTGAATATTACGCCCAGCGCGCCGACGCGGGCCTGC
TCATCACCGAGGCCTCGCAGATCTCCCAGCAGGGCCAGGGCTATCAGGACACCCCCGGCATCTACACCGACGCCCAGGTC
GAGGGCTGGAAGGCCGTGACCGACGCCGTGCATGCCCGCGGCGGGCACATCGTCATCCAGCTCTGGCACGTGGGCCGGGT
CTCCCACACGTCGCTGCAGCCGAACGGCGGCGCGCCGGTGGCGCCTTCCGCCATCACGGCCGCGACCAAGACCTTCGTGA
ACAACGCCTTCGCCGACACCTCCGAGCCCCGCGCGCTGGAGCGCTCCGAGATCCCCGGCATCCTTGAGGATTATCGCCGC
GCCGCCGCCAACGCCATCAAGGCCGGCTTCGACGGGGTGGAGATCCACGGCGCCAATGGCTACCTGATCGACCAGTTCCT
GAAGGATGGCTCGAACAAGCGCACCGACGACTACGGCGGCTCCATCGAGAACCGCGCGCGCTTCCTGCTGGAGGTGGTGG
ACACCATCGTCGCCGAGATCGGCGCCAACCGCACCGGCCTGCGCCTGTCGCCGGTATCCCCGGCCAACGGCATCAGCGAC
AGCAACCCGCAGGCCCTCTTCAACCATGTGGCCGAGCAGCTGGAAAAGCGCCACCTGCTCTTCGTCCATGTGGTGGAAGG
TGCCACCGGCGGCCCGCGCGACGTGGCGCCGGACTTCTCCTTCGATGCGCTGCGCCAGCGCTTCACCGGCCCCTGGATCG
TCAACAACGGCTATGACCTGGAGCTGGCCCAGAGCGCGATCGGCGCTGGCAAGGCGGACCTCGTCGCCTTCGGCAAGCCG
TTCATCGCCAATCCGGACCTGGTGGAGCGCCTGCGCCGCAATGCGCCGCTGAACGAGCCCGACCGCGACACCTTCTACGG
CGGCGGCGCCAAGGGCTATACCGACTATCCCACCCTTGCGGACGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
GCGTGGGAAATAGCGAGCAGTCTGACCTTGGCTGGGACGCGATCGCTCCTATGTGTGCTGCATCGCAACACTCCCACTTG
CCCTTCCGGAGGGTATCATC

Downstream 100 bases:

>100_bases
CCACCGACGCCGGCTGGGGTTTCAAATGGAACGTGGACCGGCGCATCGCGCCGGTCCATCGCCTTGAGCTGCGCGCCGAG
CCGGGCGGCTGGCCGGAACT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSAPTLFDSFRLGDMTLPNRIVMAPLTRNRAGAGFVPSPLAPEYYAQRADAGLLITEASQISQQGQGYQDTPGIYTDAQV
EGWKAVTDAVHARGGHIVIQLWHVGRVSHTSLQPNGGAPVAPSAITAATKTFVNNAFADTSEPRALERSEIPGILEDYRR
AAANAIKAGFDGVEIHGANGYLIDQFLKDGSNKRTDDYGGSIENRARFLLEVVDTIVAEIGANRTGLRLSPVSPANGISD
SNPQALFNHVAEQLEKRHLLFVHVVEGATGGPRDVAPDFSFDALRQRFTGPWIVNNGYDLELAQSAIGAGKADLVAFGKP
FIANPDLVERLRRNAPLNEPDRDTFYGGGAKGYTDYPTLADAAA

Sequences:

>Translated_364_residues
MSAPTLFDSFRLGDMTLPNRIVMAPLTRNRAGAGFVPSPLAPEYYAQRADAGLLITEASQISQQGQGYQDTPGIYTDAQV
EGWKAVTDAVHARGGHIVIQLWHVGRVSHTSLQPNGGAPVAPSAITAATKTFVNNAFADTSEPRALERSEIPGILEDYRR
AAANAIKAGFDGVEIHGANGYLIDQFLKDGSNKRTDDYGGSIENRARFLLEVVDTIVAEIGANRTGLRLSPVSPANGISD
SNPQALFNHVAEQLEKRHLLFVHVVEGATGGPRDVAPDFSFDALRQRFTGPWIVNNGYDLELAQSAIGAGKADLVAFGKP
FIANPDLVERLRRNAPLNEPDRDTFYGGGAKGYTDYPTLADAAA
>Mature_363_residues
SAPTLFDSFRLGDMTLPNRIVMAPLTRNRAGAGFVPSPLAPEYYAQRADAGLLITEASQISQQGQGYQDTPGIYTDAQVE
GWKAVTDAVHARGGHIVIQLWHVGRVSHTSLQPNGGAPVAPSAITAATKTFVNNAFADTSEPRALERSEIPGILEDYRRA
AANAIKAGFDGVEIHGANGYLIDQFLKDGSNKRTDDYGGSIENRARFLLEVVDTIVAEIGANRTGLRLSPVSPANGISDS
NPQALFNHVAEQLEKRHLLFVHVVEGATGGPRDVAPDFSFDALRQRFTGPWIVNNGYDLELAQSAIGAGKADLVAFGKPF
IANPDLVERLRRNAPLNEPDRDTFYGGGAKGYTDYPTLADAAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=51.0928961748634, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1789463, Length=346, Percent_Identity=29.7687861271676, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17565138, Length=290, Percent_Identity=27.5862068965517, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=128, Percent_Identity=42.1875, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=131, Percent_Identity=41.2213740458015, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=266, Percent_Identity=27.0676691729323, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=268, Percent_Identity=25.7462686567164, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=247, Percent_Identity=28.3400809716599, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=39.7905759162304, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=382, Percent_Identity=38.4816753926702, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39040; Mature: 38909

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTLFDSFRLGDMTLPNRIVMAPLTRNRAGAGFVPSPLAPEYYAQRADAGLLITEASQ
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECHHH
ISQQGQGYQDTPGIYTDAQVEGWKAVTDAVHARGGHIVIQLWHVGRVSHTSLQPNGGAPV
HHHHCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCC
APSAITAATKTFVNNAFADTSEPRALERSEIPGILEDYRRAAANAIKAGFDGVEIHGANG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLIDQFLKDGSNKRTDDYGGSIENRARFLLEVVDTIVAEIGANRTGLRLSPVSPANGISD
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
SNPQALFNHVAEQLEKRHLLFVHVVEGATGGPRDVAPDFSFDALRQRFTGPWIVNNGYDL
CCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCH
ELAQSAIGAGKADLVAFGKPFIANPDLVERLRRNAPLNEPDRDTFYGGGAKGYTDYPTLA
HHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
DAAA
HCCC
>Mature Secondary Structure 
SAPTLFDSFRLGDMTLPNRIVMAPLTRNRAGAGFVPSPLAPEYYAQRADAGLLITEASQ
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECHHH
ISQQGQGYQDTPGIYTDAQVEGWKAVTDAVHARGGHIVIQLWHVGRVSHTSLQPNGGAPV
HHHHCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCC
APSAITAATKTFVNNAFADTSEPRALERSEIPGILEDYRRAAANAIKAGFDGVEIHGANG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLIDQFLKDGSNKRTDDYGGSIENRARFLLEVVDTIVAEIGANRTGLRLSPVSPANGISD
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
SNPQALFNHVAEQLEKRHLLFVHVVEGATGGPRDVAPDFSFDALRQRFTGPWIVNNGYDL
CCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCH
ELAQSAIGAGKADLVAFGKPFIANPDLVERLRRNAPLNEPDRDTFYGGGAKGYTDYPTLA
HHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
DAAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]