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The map label for this gene is mhpA [H]

Identifier: 154245252

GI number: 154245252

Start: 1499449

End: 1501056

Strand: Direct

Name: mhpA [H]

Synonym: Xaut_1304

Alternate gene names: 154245252

Gene position: 1499449-1501056 (Clockwise)

Preceding gene: 154245251

Following gene: 154245253

Centisome position: 28.24

GC content: 66.67

Gene sequence:

>1608_bases
ATGATGAGCACCTATACCTATCCCAAGTATCCCTATCGCATCTCGCCCGAGCAGGAAGCCGGCCGCCTCGTGCGCCATCC
GGTGGTGGTGATCGGCGCCGGCCCGGTGGGCCTCACCGCTGCCCTCGACTTTGCCGCGCGCGGCATTCCCACCGTGGTGC
TGGACGACAACGACACCGTGTCGATGGGCTCGCGCGCTGTGTGCTACGCCAAGCGGCCGCTGGAGATCTGGGATCGCCTG
GGTTGCGGCGAGCGCATGGTGGCGCGGGGCATCAGCTGGCGCATCGGCAAGGTCTTCTTTGAAGATGACCTGCGCTACCA
GTTTGATCTTCTCCCCGAGCCGGACCACAAGATGCCGGCCATGATCAACCTTCAGCAATATTATCTGGAGGAGTTCATGG
TGGAGGAATGCGCCAGCCAGCCTCTGGTGGACCTGCGCTGGAAGCACAAGCTACTGTCCCTCCAGCAGCATGAGGATTTC
GCCACCCTCGTGGTGGAGACGCCGGACGGGGTGTTCACCCTGGAGGCGCAATGGGTGGTGGCCTGCGACGGCGCCAATTC
CGACACCCGCAAGATGGTGGGCGCCGAATTCACCGGACAGGCCTTTCAGGACCGCTTCCTCATCGCCGACGTGGTGATGA
AGGCGCAGTTTCCCACCGAGCGCTGGTTCTGGTTCGATCCCCCCTTCCATCGCAACCAGTCGGTGCTGCTGCACCGGCAG
GCGGATGATGTGTGGCGCATCGACTTCCAGCTCGGTTGGGACGCCGACCCCACCGAGGAGAAGAAGCCGGAGAACATCAT
CCCGCGGGTGAAGGCCATGCTGGGGCCGGATGCGCAGTTCGAGCTGGAATGGGCCTCGGTCTACCAGTTCGCCTGCCGGC
GCATCGACCGCTTCCGCCACGGCCGGGTGATCTTCGCCGGCGATGCCGCCCACCAGGTCTCGCCATTCGGCGCGCGCGGC
GCCAATTCCGGCGTGCAGGACGTGGACAATCTGGGCTGGAAGCTCAAGCTGGTGCTGGATGGCGCGGCGCCGGAGAGCCT
CCTCGACACCTACCATGACGAGCGGGCGCTGGCCGCCGACGACAACATCGCCAATTCCACCCGCTCTACGGATTTCATCA
CCCCCAAGAGCGCCATGAGCCGGCGCTTCCGCAATGCCGTGCTGGAGCTGGCGCAGGACCACCCCTTCGCCCGGCCGCTG
GTGAATTCCGGGCGGCTCTCCACCCCCACGCCTTATCTCGCCTCGCCCCTCAACACGCCGGACGAGGACGCCTTCGCCGG
CCTCATGAAGCCCGGTACCACCTGCGCCGATGCGCCGGTGCGGGTGGATGGCAAGGACGGCTGGTTCCTCGGCCTTTTGG
GCGACGGCTTCACCGTGGTCACCTTCGGCGATGCTCCGCCGGGCGAGGTGCGGGTAGGGGGCATCGCGGCCCCGGTGCTG
CGCATCGGCATCGATGCTGCGGATGCCAAGGGCCGCCTCGCCGAGCGCTACGACGGCGCCGATGGCACCACCTATCTCAT
CCGCCCGGACCAGCATGTGGCGGCCCGCTGGCGCAGCTTCGACCCCGACAAGATCCAGAGCGCCATCAAGCGCGCCATCG
CCGCCTAG

Upstream 100 bases:

>100_bases
AGCTGAGGGCAGGCCCCCTCGCGCCCGGACGCCGCCGGGCGCACCATCACCAATCGTCACGAGGCCGAATGGCCCGCCGC
GCTTCGGGAGAAACGCTCCG

Downstream 100 bases:

>100_bases
AGCGTTTTCCATTCGCTCCGGCTCACGGAAATCGCTCCAGACTCTCGTTCTGACGCGTTTTCTTTCTGAGAACCGGTACT
CACTTCGCTCGAAAACGCTT

Product: FAD-dependent oxidoreductase

Products: NA

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MMSTYTYPKYPYRISPEQEAGRLVRHPVVVIGAGPVGLTAALDFAARGIPTVVLDDNDTVSMGSRAVCYAKRPLEIWDRL
GCGERMVARGISWRIGKVFFEDDLRYQFDLLPEPDHKMPAMINLQQYYLEEFMVEECASQPLVDLRWKHKLLSLQQHEDF
ATLVVETPDGVFTLEAQWVVACDGANSDTRKMVGAEFTGQAFQDRFLIADVVMKAQFPTERWFWFDPPFHRNQSVLLHRQ
ADDVWRIDFQLGWDADPTEEKKPENIIPRVKAMLGPDAQFELEWASVYQFACRRIDRFRHGRVIFAGDAAHQVSPFGARG
ANSGVQDVDNLGWKLKLVLDGAAPESLLDTYHDERALAADDNIANSTRSTDFITPKSAMSRRFRNAVLELAQDHPFARPL
VNSGRLSTPTPYLASPLNTPDEDAFAGLMKPGTTCADAPVRVDGKDGWFLGLLGDGFTVVTFGDAPPGEVRVGGIAAPVL
RIGIDAADAKGRLAERYDGADGTTYLIRPDQHVAARWRSFDPDKIQSAIKRAIAA

Sequences:

>Translated_535_residues
MMSTYTYPKYPYRISPEQEAGRLVRHPVVVIGAGPVGLTAALDFAARGIPTVVLDDNDTVSMGSRAVCYAKRPLEIWDRL
GCGERMVARGISWRIGKVFFEDDLRYQFDLLPEPDHKMPAMINLQQYYLEEFMVEECASQPLVDLRWKHKLLSLQQHEDF
ATLVVETPDGVFTLEAQWVVACDGANSDTRKMVGAEFTGQAFQDRFLIADVVMKAQFPTERWFWFDPPFHRNQSVLLHRQ
ADDVWRIDFQLGWDADPTEEKKPENIIPRVKAMLGPDAQFELEWASVYQFACRRIDRFRHGRVIFAGDAAHQVSPFGARG
ANSGVQDVDNLGWKLKLVLDGAAPESLLDTYHDERALAADDNIANSTRSTDFITPKSAMSRRFRNAVLELAQDHPFARPL
VNSGRLSTPTPYLASPLNTPDEDAFAGLMKPGTTCADAPVRVDGKDGWFLGLLGDGFTVVTFGDAPPGEVRVGGIAAPVL
RIGIDAADAKGRLAERYDGADGTTYLIRPDQHVAARWRSFDPDKIQSAIKRAIAA
>Mature_535_residues
MMSTYTYPKYPYRISPEQEAGRLVRHPVVVIGAGPVGLTAALDFAARGIPTVVLDDNDTVSMGSRAVCYAKRPLEIWDRL
GCGERMVARGISWRIGKVFFEDDLRYQFDLLPEPDHKMPAMINLQQYYLEEFMVEECASQPLVDLRWKHKLLSLQQHEDF
ATLVVETPDGVFTLEAQWVVACDGANSDTRKMVGAEFTGQAFQDRFLIADVVMKAQFPTERWFWFDPPFHRNQSVLLHRQ
ADDVWRIDFQLGWDADPTEEKKPENIIPRVKAMLGPDAQFELEWASVYQFACRRIDRFRHGRVIFAGDAAHQVSPFGARG
ANSGVQDVDNLGWKLKLVLDGAAPESLLDTYHDERALAADDNIANSTRSTDFITPKSAMSRRFRNAVLELAQDHPFARPL
VNSGRLSTPTPYLASPLNTPDEDAFAGLMKPGTTCADAPVRVDGKDGWFLGLLGDGFTVVTFGDAPPGEVRVGGIAAPVL
RIGIDAADAKGRLAERYDGADGTTYLIRPDQHVAARWRSFDPDKIQSAIKRAIAA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=516, Percent_Identity=25.7751937984496, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 59672; Mature: 59672

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTYTYPKYPYRISPEQEAGRLVRHPVVVIGAGPVGLTAALDFAARGIPTVVLDDNDTV
CCCCCCCCCCCEEECCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCC
SMGSRAVCYAKRPLEIWDRLGCGERMVARGISWRIGKVFFEDDLRYQFDLLPEPDHKMPA
CCCCEEEEEECCCHHHHHHCCCCHHHHHCCCCEEEEEEEEECCCCEEEECCCCCCCCCCC
MINLQQYYLEEFMVEECASQPLVDLRWKHKLLSLQQHEDFATLVVETPDGVFTLEAQWVV
EECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEEEE
ACDGANSDTRKMVGAEFTGQAFQDRFLIADVVMKAQFPTERWFWFDPPFHRNQSVLLHRQ
EECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
ADDVWRIDFQLGWDADPTEEKKPENIIPRVKAMLGPDAQFELEWASVYQFACRRIDRFRH
CCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHC
GRVIFAGDAAHQVSPFGARGANSGVQDVDNLGWKLKLVLDGAAPESLLDTYHDERALAAD
CCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEECC
DNIANSTRSTDFITPKSAMSRRFRNAVLELAQDHPFARPLVNSGRLSTPTPYLASPLNTP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHCCCCCCC
DEDAFAGLMKPGTTCADAPVRVDGKDGWFLGLLGDGFTVVTFGDAPPGEVRVGGIAAPVL
CHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEECCCCCCCEEECCCHHHHH
RIGIDAADAKGRLAERYDGADGTTYLIRPDQHVAARWRSFDPDKIQSAIKRAIAA
HHCCCCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSTYTYPKYPYRISPEQEAGRLVRHPVVVIGAGPVGLTAALDFAARGIPTVVLDDNDTV
CCCCCCCCCCCEEECCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCC
SMGSRAVCYAKRPLEIWDRLGCGERMVARGISWRIGKVFFEDDLRYQFDLLPEPDHKMPA
CCCCEEEEEECCCHHHHHHCCCCHHHHHCCCCEEEEEEEEECCCCEEEECCCCCCCCCCC
MINLQQYYLEEFMVEECASQPLVDLRWKHKLLSLQQHEDFATLVVETPDGVFTLEAQWVV
EECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEEEE
ACDGANSDTRKMVGAEFTGQAFQDRFLIADVVMKAQFPTERWFWFDPPFHRNQSVLLHRQ
EECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
ADDVWRIDFQLGWDADPTEEKKPENIIPRVKAMLGPDAQFELEWASVYQFACRRIDRFRH
CCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHC
GRVIFAGDAAHQVSPFGARGANSGVQDVDNLGWKLKLVLDGAAPESLLDTYHDERALAAD
CCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEECC
DNIANSTRSTDFITPKSAMSRRFRNAVLELAQDHPFARPLVNSGRLSTPTPYLASPLNTP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHCCCCCCC
DEDAFAGLMKPGTTCADAPVRVDGKDGWFLGLLGDGFTVVTFGDAPPGEVRVGGIAAPVL
CHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEECCCCCCCEEECCCHHHHH
RIGIDAADAKGRLAERYDGADGTTYLIRPDQHVAARWRSFDPDKIQSAIKRAIAA
HHCCCCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA