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The map label for this gene is zraS [H]

Identifier: 154245239

GI number: 154245239

Start: 1485521

End: 1487593

Strand: Reverse

Name: zraS [H]

Synonym: Xaut_1291

Alternate gene names: 154245239

Gene position: 1487593-1485521 (Counterclockwise)

Preceding gene: 154245240

Following gene: 154245235

Centisome position: 28.02

GC content: 70.82

Gene sequence:

>2073_bases
ATGCCTGAACCGGCGGCGCAGGCCGCGCCGAGGCATTTGTTCTCCCGCCTGCGCTCGGTGCGGGCGCGGCTGCTGGCCAT
CGCGCTGTTGCCGACGCTGATCATCATGCCGGCGGTGCTGGCGCTGGTGGTGACATGGTGGACCCGCCAGTTCGACCACC
TGCTCATTTCCAAGGTGGCGGGCGACCTCACCATCGCGCGGCAATATCTCGACCGCCTCATGGAGCGTTCCGACGAGACC
CTGCACGCGCTCGGGGAGTCCGTCGCCTTCGCGCGGGTGGCGGAGGCCGGCGATGGCGCCGCCATGGCCGATTTCCTCGA
TGCCCGCCGCCGCGCCATGGGACTCGATTTCCTTTACCTGCTGGATCCGCAGGGCCGCCGCATCGCCGCCTCCCCGCCCT
CGGTGCAGGATGGCCCGCAGGCGCACCTGTGGCTGGTGGAGCGCCGGGCACTGGAGGGGCAGGCCTCCACGCAGATCGAC
ATCTTCGGCCCGGAGCAGCTTGCCCGTCTCTCCCCCGAGCTGGCGGCGCGCGCCCGCATCGCCCTCGTGCCCACCCCCGG
CGCCGTGCCCACCGCCCGCGATGCCGAAGACCGGGGCATGGTGGTGCAGTCCGGCACCCCGGTGCAGCTTGCGGACGGCA
CGCGGAAGGGCGCGCTGGTGGGTGGCATCCTGCTCAACCAGAACCTGGACTTCATCGACACCATCAATGACCTGGTCTAC
CGCCCCGGCAGCCTGCCGGAAGGCTCAGCCGGCACCGCCACCCTGTTCGTGGAGGACGTGCGCGTCTCCACCAACGTGCG
CCTGTTCGAGGGCCGCCGGGCGCTGGGCACCCGCGTCTCCAAGGAGGTGCGCGAGGCGGTGCTGGGTGAGGGCCGGGTGT
GGCTCGACCGCGCCTTCGTGGTCAACGACTGGTACATCTCCGCCTATGAACCGGTGGTGGACAGCAAAGGCCAGCGCATC
GGCATGCTCTATGTGGGCTTCCTGGAGGCGCCATTCCTGGCGCTGAAGCGGGCGAGCCTCGCGGCGGTGGGCATCGGCTT
CCTGCTGGTGGCGCTCATCGCCAGTCCGGTGCTGCTGCGCTGGGCGGCGGGTATCTTCCGGCCGCTGGAGGCCATGAATG
CCACCATCGCCCAGGTGGAGGCCGGCACGCTCTCCGCCCGCACCGGGGTCGCCGCCACCTCCGACGAGATCGGCCGCGTC
GCCCACCATCTCGACGGCATGCTGGACCTGCTCCAGCAGCGCGACGCCGAGCTCAGGCGCTGGGCTCAGGAGCTCGACGG
CCGCGTCGCCGCCCGCACGGCGGAGCTGAAGGCCGCCAATGCGGAGCTCGCCGCCACCCAGCGCCAGCTGGTGATGTCGG
AGAAGCTGGCGGCCATCGGCGAGATCACCGCCGGGGTGGCCCACGAGATCAACAATCCGGTGGCGGTCATCCAGGGCAAC
CTCGATGTGGCGCGCGACGTGCTGGGGCCGGCCGCGGCTCCGGTGCGCACCGAGTTCAACCTCATCGACCAGCAGGTCCA
CCGCATCACCATCATCGTTTCGAAGCTGCTGCAGTTCGCCCGGCCGGCGGAATTTGCCGGCCACCTGGAGCCGGTGGCGC
CGTCCGAGGTGATCGGCGATGCTTTGGTGCTGGTGCAATACCTGCTCGGGCGCGGCGAGATCGCGGTGGTGCGGGACGAG
AGCGCGACGCGCCTCGTCCTCATGAACCGGGTGGAGCTGCAGCAGGTGCTCATCAACCTGATGGTCAACGCTGCCCACGC
CATGCCCCAGGGCGGGACCCTCACCTTGCGCACCCGCGACACCGCGCACAACGGCCGCGCCGGGGTCTCCATCGCCGTGG
CCGACACCGGCCCCGGCATAGCCCCAGAGCATGTGGCGCGGGTGTTCGATCCCTTCTTCACCACCAAACCGCAGGGCGGC
GACAAGGCCGGCACCGGCCTCGGCCTCTCCATCAGCTTCACTTTGGTGGACCGGGCCGGCGGCACCATGAGCCTTGCGAG
CCCACCGGGAGGCGGCGCCACCTTCGTCCTCTGGCTGCCGCAGGCGGATGCCACCGCCGCGTGGGTCGGCTGA

Upstream 100 bases:

>100_bases
ATGCTCAAGGTGCTGGGCGAGGCCGGCGGCAACCGGGCGGAGGCGGCGCGGCGCCTCGGCGTCTCGCGCAAGACCCTCGA
CCGCAAGCTGAAGGCCTGGA

Downstream 100 bases:

>100_bases
CGGACGGCGTCCGCGCCGTTCTCAGACTAGCACGAAGGCGGCGATCATCGCGGCATAAAGGGCGAGGATGATGCCGGCGC
CCAGCGCATTGGTGCGCGGC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MPEPAAQAAPRHLFSRLRSVRARLLAIALLPTLIIMPAVLALVVTWWTRQFDHLLISKVAGDLTIARQYLDRLMERSDET
LHALGESVAFARVAEAGDGAAMADFLDARRRAMGLDFLYLLDPQGRRIAASPPSVQDGPQAHLWLVERRALEGQASTQID
IFGPEQLARLSPELAARARIALVPTPGAVPTARDAEDRGMVVQSGTPVQLADGTRKGALVGGILLNQNLDFIDTINDLVY
RPGSLPEGSAGTATLFVEDVRVSTNVRLFEGRRALGTRVSKEVREAVLGEGRVWLDRAFVVNDWYISAYEPVVDSKGQRI
GMLYVGFLEAPFLALKRASLAAVGIGFLLVALIASPVLLRWAAGIFRPLEAMNATIAQVEAGTLSARTGVAATSDEIGRV
AHHLDGMLDLLQQRDAELRRWAQELDGRVAARTAELKAANAELAATQRQLVMSEKLAAIGEITAGVAHEINNPVAVIQGN
LDVARDVLGPAAAPVRTEFNLIDQQVHRITIIVSKLLQFARPAEFAGHLEPVAPSEVIGDALVLVQYLLGRGEIAVVRDE
SATRLVLMNRVELQQVLINLMVNAAHAMPQGGTLTLRTRDTAHNGRAGVSIAVADTGPGIAPEHVARVFDPFFTTKPQGG
DKAGTGLGLSISFTLVDRAGGTMSLASPPGGGATFVLWLPQADATAAWVG

Sequences:

>Translated_690_residues
MPEPAAQAAPRHLFSRLRSVRARLLAIALLPTLIIMPAVLALVVTWWTRQFDHLLISKVAGDLTIARQYLDRLMERSDET
LHALGESVAFARVAEAGDGAAMADFLDARRRAMGLDFLYLLDPQGRRIAASPPSVQDGPQAHLWLVERRALEGQASTQID
IFGPEQLARLSPELAARARIALVPTPGAVPTARDAEDRGMVVQSGTPVQLADGTRKGALVGGILLNQNLDFIDTINDLVY
RPGSLPEGSAGTATLFVEDVRVSTNVRLFEGRRALGTRVSKEVREAVLGEGRVWLDRAFVVNDWYISAYEPVVDSKGQRI
GMLYVGFLEAPFLALKRASLAAVGIGFLLVALIASPVLLRWAAGIFRPLEAMNATIAQVEAGTLSARTGVAATSDEIGRV
AHHLDGMLDLLQQRDAELRRWAQELDGRVAARTAELKAANAELAATQRQLVMSEKLAAIGEITAGVAHEINNPVAVIQGN
LDVARDVLGPAAAPVRTEFNLIDQQVHRITIIVSKLLQFARPAEFAGHLEPVAPSEVIGDALVLVQYLLGRGEIAVVRDE
SATRLVLMNRVELQQVLINLMVNAAHAMPQGGTLTLRTRDTAHNGRAGVSIAVADTGPGIAPEHVARVFDPFFTTKPQGG
DKAGTGLGLSISFTLVDRAGGTMSLASPPGGGATFVLWLPQADATAAWVG
>Mature_689_residues
PEPAAQAAPRHLFSRLRSVRARLLAIALLPTLIIMPAVLALVVTWWTRQFDHLLISKVAGDLTIARQYLDRLMERSDETL
HALGESVAFARVAEAGDGAAMADFLDARRRAMGLDFLYLLDPQGRRIAASPPSVQDGPQAHLWLVERRALEGQASTQIDI
FGPEQLARLSPELAARARIALVPTPGAVPTARDAEDRGMVVQSGTPVQLADGTRKGALVGGILLNQNLDFIDTINDLVYR
PGSLPEGSAGTATLFVEDVRVSTNVRLFEGRRALGTRVSKEVREAVLGEGRVWLDRAFVVNDWYISAYEPVVDSKGQRIG
MLYVGFLEAPFLALKRASLAAVGIGFLLVALIASPVLLRWAAGIFRPLEAMNATIAQVEAGTLSARTGVAATSDEIGRVA
HHLDGMLDLLQQRDAELRRWAQELDGRVAARTAELKAANAELAATQRQLVMSEKLAAIGEITAGVAHEINNPVAVIQGNL
DVARDVLGPAAAPVRTEFNLIDQQVHRITIIVSKLLQFARPAEFAGHLEPVAPSEVIGDALVLVQYLLGRGEIAVVRDES
ATRLVLMNRVELQQVLINLMVNAAHAMPQGGTLTLRTRDTAHNGRAGVSIAVADTGPGIAPEHVARVFDPFFTTKPQGGD
KAGTGLGLSISFTLVDRAGGTMSLASPPGGGATFVLWLPQADATAAWVG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=35.7446808510638, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=32.4894514767932, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789149, Length=440, Percent_Identity=23.1818181818182, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082128, Length=267, Percent_Identity=26.9662921348315, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=27.4774774774775, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=303, Percent_Identity=30.03300330033, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=29.8039215686275, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=29.3877551020408, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI145693157, Length=223, Percent_Identity=30.9417040358744, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=25.5411255411255, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790346, Length=250, Percent_Identity=23.6, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73856; Mature: 73725

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPAAQAAPRHLFSRLRSVRARLLAIALLPTLIIMPAVLALVVTWWTRQFDHLLISKVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLTIARQYLDRLMERSDETLHALGESVAFARVAEAGDGAAMADFLDARRRAMGLDFLYL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEE
LDPQGRRIAASPPSVQDGPQAHLWLVERRALEGQASTQIDIFGPEQLARLSPELAARARI
ECCCCCEEECCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECHHHHHHCCHHHHCCEEE
ALVPTPGAVPTARDAEDRGMVVQSGTPVQLADGTRKGALVGGILLNQNLDFIDTINDLVY
EEEECCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHC
RPGSLPEGSAGTATLFVEDVRVSTNVRLFEGRRALGTRVSKEVREAVLGEGRVWLDRAFV
CCCCCCCCCCCEEEEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
VNDWYISAYEPVVDSKGQRIGMLYVGFLEAPFLALKRASLAAVGIGFLLVALIASPVLLR
EECEEEECCCHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAAGIFRPLEAMNATIAQVEAGTLSARTGVAATSDEIGRVAHHLDGMLDLLQQRDAELRR
HHHHHHHHHHHHCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
WAQELDGRVAARTAELKAANAELAATQRQLVMSEKLAAIGEITAGVAHEINNPVAVIQGN
HHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
LDVARDVLGPAAAPVRTEFNLIDQQVHRITIIVSKLLQFARPAEFAGHLEPVAPSEVIGD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
ALVLVQYLLGRGEIAVVRDESATRLVLMNRVELQQVLINLMVNAAHAMPQGGTLTLRTRD
HHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
TAHNGRAGVSIAVADTGPGIAPEHVARVFDPFFTTKPQGGDKAGTGLGLSISFTLVDRAG
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCC
GTMSLASPPGGGATFVLWLPQADATAAWVG
CEEEECCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PEPAAQAAPRHLFSRLRSVRARLLAIALLPTLIIMPAVLALVVTWWTRQFDHLLISKVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLTIARQYLDRLMERSDETLHALGESVAFARVAEAGDGAAMADFLDARRRAMGLDFLYL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEE
LDPQGRRIAASPPSVQDGPQAHLWLVERRALEGQASTQIDIFGPEQLARLSPELAARARI
ECCCCCEEECCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEECHHHHHHCCHHHHCCEEE
ALVPTPGAVPTARDAEDRGMVVQSGTPVQLADGTRKGALVGGILLNQNLDFIDTINDLVY
EEEECCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHC
RPGSLPEGSAGTATLFVEDVRVSTNVRLFEGRRALGTRVSKEVREAVLGEGRVWLDRAFV
CCCCCCCCCCCEEEEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
VNDWYISAYEPVVDSKGQRIGMLYVGFLEAPFLALKRASLAAVGIGFLLVALIASPVLLR
EECEEEECCCHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAAGIFRPLEAMNATIAQVEAGTLSARTGVAATSDEIGRVAHHLDGMLDLLQQRDAELRR
HHHHHHHHHHHHCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
WAQELDGRVAARTAELKAANAELAATQRQLVMSEKLAAIGEITAGVAHEINNPVAVIQGN
HHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
LDVARDVLGPAAAPVRTEFNLIDQQVHRITIIVSKLLQFARPAEFAGHLEPVAPSEVIGD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
ALVLVQYLLGRGEIAVVRDESATRLVLMNRVELQQVLINLMVNAAHAMPQGGTLTLRTRD
HHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
TAHNGRAGVSIAVADTGPGIAPEHVARVFDPFFTTKPQGGDKAGTGLGLSISFTLVDRAG
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCC
GTMSLASPPGGGATFVLWLPQADATAAWVG
CEEEECCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]