Click here to switch to the map view.

The map label for this gene is dnaG

Identifier: 154245187

GI number: 154245187

Start: 1425407

End: 1427326

Strand: Reverse

Name: dnaG

Synonym: Xaut_1239

Alternate gene names: 154245187

Gene position: 1427326-1425407 (Counterclockwise)

Preceding gene: 154245188

Following gene: 154245186

Centisome position: 26.89

GC content: 68.85

Gene sequence:

>1920_bases
ATGCGCTTTCCGCCCGCCTTCCTCGACGAGATCCGTGCCCGCCTTCCGGTTTCGGAGGTGGTGGGCCGGCGCGTCAAGCT
GAAGAAGCAGGGGCGTGAGTTCGCTGGCCTCTCGCCTTTCAACGCGGAGAAGACGCCGTCCTTCTTCGTCAATGATCAGA
AGGGCTTCTACCACTGCTTCTCCTCCGGCAAGCACGGCGACGTGTTCGACTTCCTGATGGAGACCGAGGGCGTGCCCTTC
GGCGAGGCAGTGGAGCGGCTTGCCGCAATGGCCGGCCTGCCCCTGCCGCAGGAGACCCCCGAAGCCGCCGCCAAGGAGCG
CCGCCGCCACACCCTGTTCGAGGTCATGTCCCTTGCCGCCCGGCATTTCGAGGAGACGCTGCAATCGCGCCTCGGCGCCA
AGGCCCGCGGTTACCTGGCTGATCGGGAACTGGGCGCTGCCACCCAGAAGCGATTCGGTCTCGGCTATGCCGCCGGCGAG
CGCTTCCGCCTGAAGGAGGCGCTGGGCAAGGCCGGCGTGCCGGTGGAGGACATGGTGGAAGCCGGCCTGCTGATTGCCGG
CGACGACATTCCCGTGCCCTACGACCGCTTCCGCGACCGGGTGATGTTTCCCATCACCGACCTGAAGGGCCGCATCGTCG
GCTTCGGCGGCCGGGCGCTGGAGAAGGATGTTCCGGCCAAATACCTCAACTCCCCGGAGACGGTGCTCTTTCACAAGGGA
TCGCTGCTTTATAACGGCGCCGCCGCCCGCACCGCCGCCCAGTCGGGGGCGCGGGTGATCGCGGTGGAAGGCTATGTGGA
CGTGATCGCCATGGCCGAGGCCGGCTTCGGCGCCACCGTGGCCCCGCTGGGCACGGCACTGACCGAGGAGCAATTGCAAC
TGCTCTGGCGCATGTCGGAGGAGCCGCTGCTGCTGTTCGACGGCGACAAGGCCGGCCGCCGAGCCGCCCATCGCGCCATC
GATCTCGCCCTGCCGCACCTGAAGGCCGGCCGCAGCCTCGCCTTCGCCACCCTGCCGGAGGGGCAGGACCCCGACGACCT
CATCCGCCGCTCCGGCCGCGATGCCATGGAAGCCGTGCTGGCCCAGGCGCGGCCGCTGGTGGCGGAACTGTGGGCGCGCG
AGAGCGAAGCCGCCACCCTCGACACGCCGGAACGCCGCGCCGGCCTCGAAGCCCGCCTCGGCGAGGTGGTGTCCGCCATC
GCCGACGAGACGGTGCGCAAGCACTACCGCCAAGAATTGCTCGGCCGCTTCAACGCCCTGGTTTCCCCGCAGGACCGAGC
CCCAGCCCGTGCCCGGCGGGAGGGCTGGAAGGACGGCTGGCAGCCGTCCGGCGGTCGCCGCGGAACGCCTTTGCCCCCGC
CCCTCGTGCCGCGCGGCACCGAGATCGCCCGCAGCGCGCTGGTGCGCGGGCCCATGGCGGCCCTGCCCCGAAACGAGGCC
CTGCTGCTGTTCTGCCTCATCAACCACACTTTCCTGCTGGACGAGGCCGACGAAGACGTGGCAAGCCTACCGCTGGCCAA
CCCGGAGGCGGATCGTCTGCGCCGCATCCTGATCGAAGCCCATCTTGAAGGCGCCGACGCCGACGCCCTCACCGAGCGCC
TGGGGCGGGCAGATATCGACGCCCTCACCCGCCGGGTGAAGGCACTCGCCAATCCCCGGCACGACTGGCCGGCATATCCA
GATACCGCGCAGAATGATGTCCGGCTCTGGTGGCATCAGCGGACGGCCTTGCATCGCAAGGCCCACGCGCTATCTAGAGA
GTTGAAGGAGGCCGAGCGCAGTCTGGGAGAAGATCCCAGTGAAGCCAATTTTGCCTGGCTTCGGGACGTCCGCGAGCGCC
TTTCTGCGCTGGATGGAACCGAAGCACTGGTGGAAGGCTTTGGTGCGTCATCAGGCCGGAGTGCGGTACGCTCCCTTTGA

Upstream 100 bases:

>100_bases
GTCTCGTCCGCCGTGCCTCTCATGCGGCCTCGGCGGCCACAGGCGCTGACGCCGCGACACCGCGCCGACGCCCCAACAGA
CCAGGCACAGACCAAGCCAT

Downstream 100 bases:

>100_bases
ACTGCCCATGTTCCAGGAACCGGATTCCGGGAATAAAGGTGGTTCGAGGCGGATTACGGCACTCTTCTCGCCTTTCGGCA
GTCGGCCACCCCGTGGCGGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MRFPPAFLDEIRARLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGVPF
GEAVERLAAMAGLPLPQETPEAAAKERRRHTLFEVMSLAARHFEETLQSRLGAKARGYLADRELGAATQKRFGLGYAAGE
RFRLKEALGKAGVPVEDMVEAGLLIAGDDIPVPYDRFRDRVMFPITDLKGRIVGFGGRALEKDVPAKYLNSPETVLFHKG
SLLYNGAAARTAAQSGARVIAVEGYVDVIAMAEAGFGATVAPLGTALTEEQLQLLWRMSEEPLLLFDGDKAGRRAAHRAI
DLALPHLKAGRSLAFATLPEGQDPDDLIRRSGRDAMEAVLAQARPLVAELWARESEAATLDTPERRAGLEARLGEVVSAI
ADETVRKHYRQELLGRFNALVSPQDRAPARARREGWKDGWQPSGGRRGTPLPPPLVPRGTEIARSALVRGPMAALPRNEA
LLLFCLINHTFLLDEADEDVASLPLANPEADRLRRILIEAHLEGADADALTERLGRADIDALTRRVKALANPRHDWPAYP
DTAQNDVRLWWHQRTALHRKAHALSRELKEAERSLGEDPSEANFAWLRDVRERLSALDGTEALVEGFGASSGRSAVRSL

Sequences:

>Translated_639_residues
MRFPPAFLDEIRARLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGVPF
GEAVERLAAMAGLPLPQETPEAAAKERRRHTLFEVMSLAARHFEETLQSRLGAKARGYLADRELGAATQKRFGLGYAAGE
RFRLKEALGKAGVPVEDMVEAGLLIAGDDIPVPYDRFRDRVMFPITDLKGRIVGFGGRALEKDVPAKYLNSPETVLFHKG
SLLYNGAAARTAAQSGARVIAVEGYVDVIAMAEAGFGATVAPLGTALTEEQLQLLWRMSEEPLLLFDGDKAGRRAAHRAI
DLALPHLKAGRSLAFATLPEGQDPDDLIRRSGRDAMEAVLAQARPLVAELWARESEAATLDTPERRAGLEARLGEVVSAI
ADETVRKHYRQELLGRFNALVSPQDRAPARARREGWKDGWQPSGGRRGTPLPPPLVPRGTEIARSALVRGPMAALPRNEA
LLLFCLINHTFLLDEADEDVASLPLANPEADRLRRILIEAHLEGADADALTERLGRADIDALTRRVKALANPRHDWPAYP
DTAQNDVRLWWHQRTALHRKAHALSRELKEAERSLGEDPSEANFAWLRDVRERLSALDGTEALVEGFGASSGRSAVRSL
>Mature_639_residues
MRFPPAFLDEIRARLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGVPF
GEAVERLAAMAGLPLPQETPEAAAKERRRHTLFEVMSLAARHFEETLQSRLGAKARGYLADRELGAATQKRFGLGYAAGE
RFRLKEALGKAGVPVEDMVEAGLLIAGDDIPVPYDRFRDRVMFPITDLKGRIVGFGGRALEKDVPAKYLNSPETVLFHKG
SLLYNGAAARTAAQSGARVIAVEGYVDVIAMAEAGFGATVAPLGTALTEEQLQLLWRMSEEPLLLFDGDKAGRRAAHRAI
DLALPHLKAGRSLAFATLPEGQDPDDLIRRSGRDAMEAVLAQARPLVAELWARESEAATLDTPERRAGLEARLGEVVSAI
ADETVRKHYRQELLGRFNALVSPQDRAPARARREGWKDGWQPSGGRRGTPLPPPLVPRGTEIARSALVRGPMAALPRNEA
LLLFCLINHTFLLDEADEDVASLPLANPEADRLRRILIEAHLEGADADALTERLGRADIDALTRRVKALANPRHDWPAYP
DTAQNDVRLWWHQRTALHRKAHALSRELKEAERSLGEDPSEANFAWLRDVRERLSALDGTEALVEGFGASSGRSAVRSL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=473, Percent_Identity=39.7463002114165, Blast_Score=334, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70306; Mature: 70306

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPPAFLDEIRARLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCF
CCCCHHHHHHHHHHCCHHHHHCHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCHHHHH
SSGKHGDVFDFLMETEGVPFGEAVERLAAMAGLPLPQETPEAAAKERRRHTLFEVMSLAA
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHFEETLQSRLGAKARGYLADRELGAATQKRFGLGYAAGERFRLKEALGKAGVPVEDMVE
HHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHH
AGLLIAGDDIPVPYDRFRDRVMFPITDLKGRIVGFGGRALEKDVPAKYLNSPETVLFHKG
CCCEEECCCCCCCHHHHCCCEEEEEECCCCCEEECCCHHHHHCCCHHHCCCCCEEEEECC
SLLYNGAAARTAAQSGARVIAVEGYVDVIAMAEAGFGATVAPLGTALTEEQLQLLWRMSE
CEEECCHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EPLLLFDGDKAGRRAAHRAIDLALPHLKAGRSLAFATLPEGQDPDDLIRRSGRDAMEAVL
CCEEEECCCHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHH
AQARPLVAELWARESEAATLDTPERRAGLEARLGEVVSAIADETVRKHYRQELLGRFNAL
HHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSPQDRAPARARREGWKDGWQPSGGRRGTPLPPPLVPRGTEIARSALVRGPMAALPRNEA
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCC
LLLFCLINHTFLLDEADEDVASLPLANPEADRLRRILIEAHLEGADADALTERLGRADID
EEEEEECCCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHH
ALTRRVKALANPRHDWPAYPDTAQNDVRLWWHQRTALHRKAHALSRELKEAERSLGEDPS
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EANFAWLRDVRERLSALDGTEALVEGFGASSGRSAVRSL
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure
MRFPPAFLDEIRARLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCF
CCCCHHHHHHHHHHCCHHHHHCHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCHHHHH
SSGKHGDVFDFLMETEGVPFGEAVERLAAMAGLPLPQETPEAAAKERRRHTLFEVMSLAA
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHFEETLQSRLGAKARGYLADRELGAATQKRFGLGYAAGERFRLKEALGKAGVPVEDMVE
HHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCCCCCHHHHHH
AGLLIAGDDIPVPYDRFRDRVMFPITDLKGRIVGFGGRALEKDVPAKYLNSPETVLFHKG
CCCEEECCCCCCCHHHHCCCEEEEEECCCCCEEECCCHHHHHCCCHHHCCCCCEEEEECC
SLLYNGAAARTAAQSGARVIAVEGYVDVIAMAEAGFGATVAPLGTALTEEQLQLLWRMSE
CEEECCHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EPLLLFDGDKAGRRAAHRAIDLALPHLKAGRSLAFATLPEGQDPDDLIRRSGRDAMEAVL
CCEEEECCCHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHH
AQARPLVAELWARESEAATLDTPERRAGLEARLGEVVSAIADETVRKHYRQELLGRFNAL
HHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSPQDRAPARARREGWKDGWQPSGGRRGTPLPPPLVPRGTEIARSALVRGPMAALPRNEA
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCC
LLLFCLINHTFLLDEADEDVASLPLANPEADRLRRILIEAHLEGADADALTERLGRADID
EEEEEECCCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHH
ALTRRVKALANPRHDWPAYPDTAQNDVRLWWHQRTALHRKAHALSRELKEAERSLGEDPS
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EANFAWLRDVRERLSALDGTEALVEGFGASSGRSAVRSL
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]