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The map label for this gene is xylX [H]

Identifier: 154245082

GI number: 154245082

Start: 1305157

End: 1306545

Strand: Direct

Name: xylX [H]

Synonym: Xaut_1134

Alternate gene names: 154245082

Gene position: 1305157-1306545 (Clockwise)

Preceding gene: 154245081

Following gene: 154245083

Centisome position: 24.58

GC content: 64.79

Gene sequence:

>1389_bases
ATGAACGCCCACGCTCCGCTCCCCGATTATGCCGCCCTTGCCGCCCGCCTCGAAGGCGCGGTGGTGGAAGACCCGGCCAA
GGGCCTCTATCGCTGCCGCCGCGACATCTTCACCGACCCCGACCTGTTCGAGCTGGAGATGACGCACATCTTCGAGGGCA
ACTGGATTTATCTTGCCCACGAGAGCCAGATCCCCAATCCGAACGATTATTTCACCACCTTCATCGGCCGCCAGCCCATC
GTCATCACCCGCGACCGCAAGGGCGAACTGCATGCTTTGGTGAATGCCTGCTCCCATCGCGGCGCCATGCTGTGCCGGCA
CAAGCGCGGCAACAGGGCGACCTATACCTGCCCGTTCCACGGCTGGACCTTCTCCAATGCCGGAAAACTGCTGAAGACCA
AGGAAGCCGACGGCGCCGGCTATCCCGAAAGCTTCAACACCAACGGCTCCCACGACCTCACCAAGGTGGCGCGGTTCGAG
AATTATCGCGGCTTCCTGTTCGGCTCGCTGAAGGAAGATGTATTGCCCCTGACCGAGCATCTGGGCGAGGCGGCCAAGAT
CATCGACATGATCGTGGACCAGTCGCCCGAAGGTCTCGAAGTGCTGCGGGGCAGCTCGACCTATGTCTATGACGGCAACT
GGAAGGTGCAGGCGGAGAACGGCGCCGACGGCTATCACGTCACCTCCGTGCACTGGAACTATGCTGCCACCGTCAACCAG
CGCAAGGCCGCGGCCGCAGCAGAAGCCGGCAAGGACGACGTGCGCGCCATGGATGCGGGCAGCTGGGCCAAGCAGGGCGG
CGGCTTCTATTCCTTCGACAACGGCCACCTGCTGCTATGGAGCCGCTGGGCCAACCCGGAGGATCGGCCGCTCTACGACC
GCCGCGCGGAGCTGGCGGAAAAGTTCGGCGAGGCAATGGCCGACTGGATGATCAGCCGCTCGCGCAACCTGTGCCTCTAC
CCCAACGTCTATCTGATGGACCAGTTCTCCTCGCAGATCCGCACCTTCCGGCCCATCAGCGTGGATCAGACCGATGTCAC
CATCTACTGCATCGCCCCCAAGGGCGAGAGCGCCGAGGCGCGGGCCCATCGCATCCGCCAGTATGAGGACTTCTTCAACG
CCTCCGGCATGGCGACGCCCGACGACCTGGAGGAATTCCGCTCCTGCCAGATGGGCTTCATGGCCACCTCCGCGCCGTGG
AACGACCTGTCGCGCGGCGCCACCCACTGGATCGAGGGGGCGGACGAGGGCGCCGCCGCCATCGGCCTGAAGCCGCTCCT
CTCCGGTGCCAAGACCGAGGACGAGGGCCTGTTCGTGGTCCAGCACCGCTACTGGAAGGACACTTTGGCCCGCGCCGTCG
CTGCGACGGATGCCTCCGAGACGCCCTGA

Upstream 100 bases:

>100_bases
GCGGCCTGAGCCGGGAGCGCATCGCCTACAGCCATTCCCGCAACGATCCGGACGGCCCGTCGCACCGACGGCGGGCCGAG
CCGGCAGAGAGGAAACGCCA

Downstream 100 bases:

>100_bases
CAGCCCAATGCCTTAACGAGAGACGGCGCGCCTTCAGGAGCCGCCGTCCGGGAGGAAACAATGAACGTGACCCACGAGGC
CGTGGCGGCCTTCCTCTATC

Product: ring hydroxylating dioxygenase subunit alpha

Products: 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNAHAPLPDYAALAARLEGAVVEDPAKGLYRCRRDIFTDPDLFELEMTHIFEGNWIYLAHESQIPNPNDYFTTFIGRQPI
VITRDRKGELHALVNACSHRGAMLCRHKRGNRATYTCPFHGWTFSNAGKLLKTKEADGAGYPESFNTNGSHDLTKVARFE
NYRGFLFGSLKEDVLPLTEHLGEAAKIIDMIVDQSPEGLEVLRGSSTYVYDGNWKVQAENGADGYHVTSVHWNYAATVNQ
RKAAAAAEAGKDDVRAMDAGSWAKQGGGFYSFDNGHLLLWSRWANPEDRPLYDRRAELAEKFGEAMADWMISRSRNLCLY
PNVYLMDQFSSQIRTFRPISVDQTDVTIYCIAPKGESAEARAHRIRQYEDFFNASGMATPDDLEEFRSCQMGFMATSAPW
NDLSRGATHWIEGADEGAAAIGLKPLLSGAKTEDEGLFVVQHRYWKDTLARAVAATDASETP

Sequences:

>Translated_462_residues
MNAHAPLPDYAALAARLEGAVVEDPAKGLYRCRRDIFTDPDLFELEMTHIFEGNWIYLAHESQIPNPNDYFTTFIGRQPI
VITRDRKGELHALVNACSHRGAMLCRHKRGNRATYTCPFHGWTFSNAGKLLKTKEADGAGYPESFNTNGSHDLTKVARFE
NYRGFLFGSLKEDVLPLTEHLGEAAKIIDMIVDQSPEGLEVLRGSSTYVYDGNWKVQAENGADGYHVTSVHWNYAATVNQ
RKAAAAAEAGKDDVRAMDAGSWAKQGGGFYSFDNGHLLLWSRWANPEDRPLYDRRAELAEKFGEAMADWMISRSRNLCLY
PNVYLMDQFSSQIRTFRPISVDQTDVTIYCIAPKGESAEARAHRIRQYEDFFNASGMATPDDLEEFRSCQMGFMATSAPW
NDLSRGATHWIEGADEGAAAIGLKPLLSGAKTEDEGLFVVQHRYWKDTLARAVAATDASETP
>Mature_462_residues
MNAHAPLPDYAALAARLEGAVVEDPAKGLYRCRRDIFTDPDLFELEMTHIFEGNWIYLAHESQIPNPNDYFTTFIGRQPI
VITRDRKGELHALVNACSHRGAMLCRHKRGNRATYTCPFHGWTFSNAGKLLKTKEADGAGYPESFNTNGSHDLTKVARFE
NYRGFLFGSLKEDVLPLTEHLGEAAKIIDMIVDQSPEGLEVLRGSSTYVYDGNWKVQAENGADGYHVTSVHWNYAATVNQ
RKAAAAAEAGKDDVRAMDAGSWAKQGGGFYSFDNGHLLLWSRWANPEDRPLYDRRAELAEKFGEAMADWMISRSRNLCLY
PNVYLMDQFSSQIRTFRPISVDQTDVTIYCIAPKGESAEARAHRIRQYEDFFNASGMATPDDLEEFRSCQMGFMATSAPW
NDLSRGATHWIEGADEGAAAIGLKPLLSGAKTEDEGLFVVQHRYWKDTLARAVAATDASETP

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=366, Percent_Identity=28.6885245901639, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788103, Length=205, Percent_Identity=30.2439024390244, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.12.10

Molecular weight: Translated: 51632; Mature: 51632

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHAPLPDYAALAARLEGAVVEDPAKGLYRCRRDIFTDPDLFELEMTHIFEGNWIYLAH
CCCCCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEE
ESQIPNPNDYFTTFIGRQPIVITRDRKGELHALVNACSHRGAMLCRHKRGNRATYTCPFH
CCCCCCCCCHHHHEECCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCC
GWTFSNAGKLLKTKEADGAGYPESFNTNGSHDLTKVARFENYRGFLFGSLKEDVLPLTEH
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
LGEAAKIIDMIVDQSPEGLEVLRGSSTYVYDGNWKVQAENGADGYHVTSVHWNYAATVNQ
HHHHHHHHHHHHCCCCCCHHHEECCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEECCH
RKAAAAAEAGKDDVRAMDAGSWAKQGGGFYSFDNGHLLLWSRWANPEDRPLYDRRAELAE
HHHHHHHHCCCHHHHHCCCCCHHHCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHHHHH
KFGEAMADWMISRSRNLCLYPNVYLMDQFSSQIRTFRPISVDQTDVTIYCIAPKGESAEA
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCHHH
RAHRIRQYEDFFNASGMATPDDLEEFRSCQMGFMATSAPWNDLSRGATHWIEGADEGAAA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHCCHHHHHCCCCCCCHH
IGLKPLLSGAKTEDEGLFVVQHRYWKDTLARAVAATDASETP
CCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAHAPLPDYAALAARLEGAVVEDPAKGLYRCRRDIFTDPDLFELEMTHIFEGNWIYLAH
CCCCCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEE
ESQIPNPNDYFTTFIGRQPIVITRDRKGELHALVNACSHRGAMLCRHKRGNRATYTCPFH
CCCCCCCCCHHHHEECCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCC
GWTFSNAGKLLKTKEADGAGYPESFNTNGSHDLTKVARFENYRGFLFGSLKEDVLPLTEH
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
LGEAAKIIDMIVDQSPEGLEVLRGSSTYVYDGNWKVQAENGADGYHVTSVHWNYAATVNQ
HHHHHHHHHHHHCCCCCCHHHEECCCEEEECCCEEEEECCCCCCEEEEEEEEEEEEECCH
RKAAAAAEAGKDDVRAMDAGSWAKQGGGFYSFDNGHLLLWSRWANPEDRPLYDRRAELAE
HHHHHHHHCCCHHHHHCCCCCHHHCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHHHHH
KFGEAMADWMISRSRNLCLYPNVYLMDQFSSQIRTFRPISVDQTDVTIYCIAPKGESAEA
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCHHH
RAHRIRQYEDFFNASGMATPDDLEEFRSCQMGFMATSAPWNDLSRGATHWIEGADEGAAA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHCCHHHHHCCCCCCCHH
IGLKPLLSGAKTEDEGLFVVQHRYWKDTLARAVAATDASETP
CCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzoate; NADH; H+; O2

Specific reaction: benzoate + NADH + H+ + O2 = 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]