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The map label for this gene is nfrA1 [H]

Identifier: 154245078

GI number: 154245078

Start: 1300965

End: 1301825

Strand: Direct

Name: nfrA1 [H]

Synonym: Xaut_1130

Alternate gene names: 154245078

Gene position: 1300965-1301825 (Clockwise)

Preceding gene: 154245077

Following gene: 154245080

Centisome position: 24.51

GC content: 71.78

Gene sequence:

>861_bases
ATGTCTGCCACTCTGTCTGAGGCCCGCCCCGCGCCGGCCCCGTCCGCCGATGCCCGCGCCCTGCTGGAGGCCCGCTACGG
TGCCGCCGAGGCGCCCGCCGGCATCCTGCTCAACGACACCATCGCCGCGCTGCTCTCCCACCGCACCGTGCGTGCCTTCT
CCGACCGTCCCCTGCCGGAGGGGCTGATCGAGACGCTGGTGGCGGCGGCGCAGTCGGCGCCGTCCTCATCCAACCTTCAG
ACCTTCAGCGTGGTGGCGGTGGAAAGCCCGGCCACCAAGGCCCGCCTCGCCGAGGTGGCGGGCGGCCAGAAGCATGTGGT
GGAAGCTCCTCTGGTGCTGGTCTGGCTCGCCGATCTCTCCCGCCTGGAGGAGATCGGCCGCCGCGCCGGGGCGCCCACCG
ATGCCCTGTCCTTCCTCGAAACCCTGTTCGTGGGCATCATCGATGCCGCGCTGGCGGCGCAGAATGCGCTGGTGGCGCTG
GAATCCCTCGGCCTCGGCGGCGTTTATCTCGGCGCCCTGCGCAACAAGCCGGAGGTGGTCGCCGAGCTGCTGGGCCTGCC
GCCCAACGTGGTGGGCGTGTTCGGCCTCGCCGTCGGCTATCCCGATCCGCAGCGCCCGGCGGATGTGAAGCCGCGCATCT
CCCAGCGCCTCGTGCTCCACCGCGAGCGCTATGACGCCAGCCTGCCGCAGGCGGCGCTGGACCATTATGAGGACGCTCTG
AAGGCGTTCCAGCACGGCCAGAATCTCCCCGAGGTGGGCTGGGCCAGCGTGGCGCTGGCCCGCGTGCGCGGGGCGGAGTC
CTTAAGCGGGCGCGACCGCATCCCGGAAGTGCTGAAGGCGCTCGGCTTCGGCCTGCGTTGA

Upstream 100 bases:

>100_bases
GTGCCATCGGGCAAACGGTCTTGTTGCTTGAAGTTCGGCACGGGAACGGTTCAGCTTGCCGCACGCTGAACCGTTCCCGC
CTCCGCATTATGGAGCTGTC

Downstream 100 bases:

>100_bases
GGAGCGACCGGCGCCGCGGTCACGGCGGCAGGAGCCGGTTCTCCGACAGCTTCAGCCAGTCGGGCTGGTCGGCGTACATC
TCGCGGATCAGCTGCTTGAT

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSATLSEARPAPAPSADARALLEARYGAAEAPAGILLNDTIAALLSHRTVRAFSDRPLPEGLIETLVAAAQSAPSSSNLQ
TFSVVAVESPATKARLAEVAGGQKHVVEAPLVLVWLADLSRLEEIGRRAGAPTDALSFLETLFVGIIDAALAAQNALVAL
ESLGLGGVYLGALRNKPEVVAELLGLPPNVVGVFGLAVGYPDPQRPADVKPRISQRLVLHRERYDASLPQAALDHYEDAL
KAFQHGQNLPEVGWASVALARVRGAESLSGRDRIPEVLKALGFGLR

Sequences:

>Translated_286_residues
MSATLSEARPAPAPSADARALLEARYGAAEAPAGILLNDTIAALLSHRTVRAFSDRPLPEGLIETLVAAAQSAPSSSNLQ
TFSVVAVESPATKARLAEVAGGQKHVVEAPLVLVWLADLSRLEEIGRRAGAPTDALSFLETLFVGIIDAALAAQNALVAL
ESLGLGGVYLGALRNKPEVVAELLGLPPNVVGVFGLAVGYPDPQRPADVKPRISQRLVLHRERYDASLPQAALDHYEDAL
KAFQHGQNLPEVGWASVALARVRGAESLSGRDRIPEVLKALGFGLR
>Mature_285_residues
SATLSEARPAPAPSADARALLEARYGAAEAPAGILLNDTIAALLSHRTVRAFSDRPLPEGLIETLVAAAQSAPSSSNLQT
FSVVAVESPATKARLAEVAGGQKHVVEAPLVLVWLADLSRLEEIGRRAGAPTDALSFLETLFVGIIDAALAAQNALVALE
SLGLGGVYLGALRNKPEVVAELLGLPPNVVGVFGLAVGYPDPQRPADVKPRISQRLVLHRERYDASLPQAALDHYEDALK
AFQHGQNLPEVGWASVALARVRGAESLSGRDRIPEVLKALGFGLR

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=204, Percent_Identity=39.2156862745098, Blast_Score=137, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 30055; Mature: 29924

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLSEARPAPAPSADARALLEARYGAAEAPAGILLNDTIAALLSHRTVRAFSDRPLPE
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCH
GLIETLVAAAQSAPSSSNLQTFSVVAVESPATKARLAEVAGGQKHVVEAPLVLVWLADLS
HHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RLEEIGRRAGAPTDALSFLETLFVGIIDAALAAQNALVALESLGLGGVYLGALRNKPEVV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
AELLGLPPNVVGVFGLAVGYPDPQRPADVKPRISQRLVLHRERYDASLPQAALDHYEDAL
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KAFQHGQNLPEVGWASVALARVRGAESLSGRDRIPEVLKALGFGLR
HHHHCCCCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SATLSEARPAPAPSADARALLEARYGAAEAPAGILLNDTIAALLSHRTVRAFSDRPLPE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCH
GLIETLVAAAQSAPSSSNLQTFSVVAVESPATKARLAEVAGGQKHVVEAPLVLVWLADLS
HHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RLEEIGRRAGAPTDALSFLETLFVGIIDAALAAQNALVALESLGLGGVYLGALRNKPEVV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
AELLGLPPNVVGVFGLAVGYPDPQRPADVKPRISQRLVLHRERYDASLPQAALDHYEDAL
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KAFQHGQNLPEVGWASVALARVRGAESLSGRDRIPEVLKALGFGLR
HHHHCCCCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]