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The map label for this gene is pcaJ [H]

Identifier: 154245067

GI number: 154245067

Start: 1289734

End: 1290387

Strand: Reverse

Name: pcaJ [H]

Synonym: Xaut_1119

Alternate gene names: 154245067

Gene position: 1290387-1289734 (Counterclockwise)

Preceding gene: 154245068

Following gene: 154245066

Centisome position: 24.31

GC content: 67.58

Gene sequence:

>654_bases
ATGAGCGACATCGCCTTCACGCCCTGGACGCGCGCCGAGATGGCGCAGCGCATCGCCGCCGACATTCCGGACGGCGCCAT
CGTCAACATCGGCATCGGCATGCCCGAGCTGGTTGCGGACTTCCTGCCCCAGGACAAGGAAATCCTGCTCCACAGCGAGA
ACGGCATCCTCGGCATGGGGCCCAAGCCCTCGCCGAACGAGGTGGACTACGACCTCATCAACGCCGGCAAGCAGCCGGTG
ACGCTGCTGGAAGGCGGCGCCTTCTTCGACCACAGCCTGTCGTTCGCCATGATGCGCGGCGGGCATCTGGACATCGCCAT
CCTCGGCGCCTTCCAGGTGGCGGTGAACGGCGATCTCGCCAACTGGGCCACCAACGATCCCAACGCCATTCCCGCCGTGG
GCGGCGCCATGGACCTCGCGGTGGGCGCCCGCAACGTGTTCGTGATGATGGAGTTGCTCACCAAGAAGGGCGAATCCAAG
CTGGTGGCGCACTGCACCTACCCGCTCACCGGCCTCGGCTGCGTCTCCCGCGTCTATACCGACCACGGGGTGATCGACAT
CACCCCCGAGGGCTTCGTGGCCCGCCAGCTTCCGCCGGGCCTGAGCCTCGAGGCGCTGAACGCGCGCGCCGGCATCGAGA
TCCGCGCCAGCTGA

Upstream 100 bases:

>100_bases
TGGAGGACATCGTTCCCCTCGGGACGCTCGATCCGGAAGACATCGTCACCCCCGGCGTGTTCGTGGACCGCGTCGTGGCC
ATCGGCACGGGAGCCGCCCA

Downstream 100 bases:

>100_bases
CCCTGCGCGGCCGGGGACGACCCGGCCGCGCCTTGCCCGACGACCCCTTGCCGCCCATTGCGTCGCACAGCCCGCCCCTT
GCGCCGGGGCGACCGGGCGG

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSDIAFTPWTRAEMAQRIAADIPDGAIVNIGIGMPELVADFLPQDKEILLHSENGILGMGPKPSPNEVDYDLINAGKQPV
TLLEGGAFFDHSLSFAMMRGGHLDIAILGAFQVAVNGDLANWATNDPNAIPAVGGAMDLAVGARNVFVMMELLTKKGESK
LVAHCTYPLTGLGCVSRVYTDHGVIDITPEGFVARQLPPGLSLEALNARAGIEIRAS

Sequences:

>Translated_217_residues
MSDIAFTPWTRAEMAQRIAADIPDGAIVNIGIGMPELVADFLPQDKEILLHSENGILGMGPKPSPNEVDYDLINAGKQPV
TLLEGGAFFDHSLSFAMMRGGHLDIAILGAFQVAVNGDLANWATNDPNAIPAVGGAMDLAVGARNVFVMMELLTKKGESK
LVAHCTYPLTGLGCVSRVYTDHGVIDITPEGFVARQLPPGLSLEALNARAGIEIRAS
>Mature_216_residues
SDIAFTPWTRAEMAQRIAADIPDGAIVNIGIGMPELVADFLPQDKEILLHSENGILGMGPKPSPNEVDYDLINAGKQPVT
LLEGGAFFDHSLSFAMMRGGHLDIAILGAFQVAVNGDLANWATNDPNAIPAVGGAMDLAVGARNVFVMMELLTKKGESKL
VAHCTYPLTGLGCVSRVYTDHGVIDITPEGFVARQLPPGLSLEALNARAGIEIRAS

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=209, Percent_Identity=43.5406698564593, Blast_Score=169, Evalue=1e-42,
Organism=Homo sapiens, GI4557817, Length=209, Percent_Identity=42.1052631578947, Blast_Score=165, Evalue=3e-41,
Organism=Escherichia coli, GI1788552, Length=199, Percent_Identity=40.2010050251256, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531517, Length=180, Percent_Identity=47.7777777777778, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI21358325, Length=209, Percent_Identity=39.2344497607655, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655794, Length=209, Percent_Identity=39.2344497607655, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22963; Mature: 22832

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIAFTPWTRAEMAQRIAADIPDGAIVNIGIGMPELVADFLPQDKEILLHSENGILGMG
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEEECC
PKPSPNEVDYDLINAGKQPVTLLEGGAFFDHSLSFAMMRGGHLDIAILGAFQVAVNGDLA
CCCCCCCCCHHHHHCCCCCEEEEECCEEECCCCEEEEECCCCEEEEEEEEEEEEECCCCC
NWATNDPNAIPAVGGAMDLAVGARNVFVMMELLTKKGESKLVAHCTYPLTGLGCVSRVYT
CCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHC
DHGVIDITPEGFVARQLPPGLSLEALNARAGIEIRAS
CCCEEEECCCCEEEECCCCCCCEEEECCCCCEEEEEC
>Mature Secondary Structure 
SDIAFTPWTRAEMAQRIAADIPDGAIVNIGIGMPELVADFLPQDKEILLHSENGILGMG
CCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEEECC
PKPSPNEVDYDLINAGKQPVTLLEGGAFFDHSLSFAMMRGGHLDIAILGAFQVAVNGDLA
CCCCCCCCCHHHHHCCCCCEEEEECCEEECCCCEEEEECCCCEEEEEEEEEEEEECCCCC
NWATNDPNAIPAVGGAMDLAVGARNVFVMMELLTKKGESKLVAHCTYPLTGLGCVSRVYT
CCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHC
DHGVIDITPEGFVARQLPPGLSLEALNARAGIEIRAS
CCCEEEECCCCEEEECCCCCCCEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]