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The map label for this gene is nasD [H]

Identifier: 154245029

GI number: 154245029

Start: 1240854

End: 1243298

Strand: Reverse

Name: nasD [H]

Synonym: Xaut_1080

Alternate gene names: 154245029

Gene position: 1243298-1240854 (Counterclockwise)

Preceding gene: 154245030

Following gene: 154245028

Centisome position: 23.42

GC content: 65.03

Gene sequence:

>2445_bases
ATGCAAAAACTGGTCGTTATAGGCGCCGGCATGGCTTCCGGCCGGGTTCTGGAACATTTGTTCGAAACCGATCCCGACGC
CTATCAGGTCACCCTCTTCAATGCTGAGCCGCGCGGCAATTACAACCGCATCATGCTGTCGCCGGTCCTGTCCGGCGAGA
AGAGCTATGAGGAAATCGTCATCCATGATGACGCCTGGTATGTCCGCCACAACGTCATCTGCCGCTTCGGCGAACGGGTT
CTCGAAGTCGACCGCGCCCGCAAGGTCGTGGTCGGCCCGAAGGGTGAGATCGCCTATGACAAGCTGCTGATCGCCACCGG
CTCAGTGCCATTTATCGTGCCGGTGGACGGCCACAAGCTGCCCGGCGTGATCTCCTATCGCGATCTCGACGATACCAACG
CGATGGTGGAAGCGGCCGGCGTGCCCGGCGCGCGCGCCGTGGTCATCGGCGGCGGACTGCTGGGTCTGGAGGCGGCCGCA
GGTCTCCGGCTGCGCGGCATGGAGGTGACGGTGGTGCACCTCGCCGGCCATCTGATGGAGCGCCAGCTCGACGAGGCCGC
CGGCTATCTGCTCAGCAAGGACCTGGAAAGGCGGGGAATCGCGGTCATCACCCGCGCCTCGACCAAGGCGATCGTGGGCA
CCGAGCGCGTCGAGGGCGTCCAGCTTGAGGACGGCACGCTCCTGCCGGCGGACCTCGTGGTGATGGCCGTGGGCATCCGG
CCCGCCGCCGACCTGGCGAAGCAGTCGGGGCTGGACGTTGGCCGCGCCGTGCGGGTGAACGCGCAGATGCAGACCTCGGA
CCCCGACATCTACGCCGTGGGCGAGTGCGTGGAATTCGAGGGAACACTGTTCGGCCTGGTCGCACCGCTTTACGACCAGG
CGAAGGTGGTCGCCAAATCGCTGCTCGGCGAGGCCGACGCCTTTGCCGTGAAGGAACTGTCCACCAAGCTCAAGGTCACC
GGCTGCGACCTGTTCAGCGCCGGCAGCTTTGCCGACGGCGCGGGACGCGAGGACATCGTCCTGCGCGATCCCGCACGGGG
CATCTACAAGCGGCTGGTGATCGAGGACGACCGCCTGGTGGGCGCGGTGATGTATGGCGACACCTCCGACAGCAACTGGT
TCTTCGGGCTCATCCGGGACGGCGTGAAGATCGCGGACATGCGCGACACGTTGATCTTCGGCCCGAGCTTCCAGGGAGGA
ACGCCGCTGGACCCTATGGCGGCCGTTGCAGCGTTGCCCGATGAGGCAGAGATCTGTGGTTGCAACGGCGTCTGCAAGGG
AACGATCACGGCGGCGATCGCGGCCAAGGGGCTGACCAGCCTCGACGACGTGCGGGCGCACACCAAGGCATCGGCGTCGT
GCGGTTCCTGCACCGGCCTCGTCGAGCAGATCATGCAGATCTCGCTCGGCGACAGCTACACGGCCGCCGCCGTGCAGCCG
ATCTGTTCCTGCACCACCCTCGGCCATGACGACGTGCGCCGGCTGATCAAGGCCAAGTCGCTGAAGACCATCCCGCAGGT
GATGCAGGAGCTGGAGTGGAAGACCTCCTGCGGCTGCGCCAAATGCCGGCCCGCGCTCAACTATTACCTCGTCTGCGACT
GGCCGGATGAATATGCCGACGACTACCAGTCCCGGTTCGTCAATGAACGCATCCACGCCAACATCCAGAAGGACGGAACC
TATTCCGTGGTTCCGCGCATGTGGGGCGGGGTGACATCGTCCAGCGAACTGCGCGCGATCGCCGATGTGGTCGACAAGTT
CGCCATTCCCGCCGTGAAGGTCACCGGCGGCCAGCGCATCGACATGCTCGGCATCAAGAAGGAAGACCTTCCGGCGGTGT
GGGCCGATCTCGGCAAGGCGGGCTTCGTCTCGGGCCAGGCCTATGCCAAGGGGCTCCGGACCGTGAAGACCTGCGTTGGC
ACCGACTGGTGCCGGTTCGGGACCCAGGATTCCACCGGGCTCGGCATCCGCATCGAGAAGTTCATGTGGGGCTCGTGGAC
CCCGGCCAAGCTCAAGATGGCGGTGTCCGGCTGTCCGCGCAATTGTGCCGAGGCCACCTGCAAGGACATCGGCGTCATCT
GCGTGGATTCCGGCTACGAGATCCATTTCGCTGGAGCCGCGGGCCTCGACATCAAGGGAACCGAGGTGCTCGGCCTGGTG
AAGAGCGAGGATGAGGCGCTCGAGCATATCGTGGCGCTCACCCAGATGTATCGCGAGCAGGGCCGCTATCTCGAGCGCAT
CTACAAGTGGGCCAAGCGGATCGGACTTGCGGAGATCCGCCAGCAGATCATGGAGGACCATGACAAGCGCCGGGCCTATT
ACGACCGCTTCGTGTTCTCCCAGAAGTTCGCGCAGGTCGATCCCTGGTCGGAGCGTGTCTCCGGCAAGGACAAGCACGAG
TTCCGGCCCATGGCGGAACTGGACATCACGCAGGCAGCGGAGTGA

Upstream 100 bases:

>100_bases
CCGGCCTGCCCGCCGGCTACGAATCGGTGGTCGCGATCCCGGTCTATCGGGGCGCCGAAGTCGCCTATGTCGTGGCCTGG
TATTCCTGAAGGAACGAGCG

Downstream 100 bases:

>100_bases
CGGTGGTGAACTGGATTGCAATCGGCTCCATCAGCGACATTCCCAGCCGCGGCGCGCGCTGCGTGACGACGCCTAACGGC
CGGATCGGCGTGTTCCGCAC

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MQKLVVIGAGMASGRVLEHLFETDPDAYQVTLFNAEPRGNYNRIMLSPVLSGEKSYEEIVIHDDAWYVRHNVICRFGERV
LEVDRARKVVVGPKGEIAYDKLLIATGSVPFIVPVDGHKLPGVISYRDLDDTNAMVEAAGVPGARAVVIGGGLLGLEAAA
GLRLRGMEVTVVHLAGHLMERQLDEAAGYLLSKDLERRGIAVITRASTKAIVGTERVEGVQLEDGTLLPADLVVMAVGIR
PAADLAKQSGLDVGRAVRVNAQMQTSDPDIYAVGECVEFEGTLFGLVAPLYDQAKVVAKSLLGEADAFAVKELSTKLKVT
GCDLFSAGSFADGAGREDIVLRDPARGIYKRLVIEDDRLVGAVMYGDTSDSNWFFGLIRDGVKIADMRDTLIFGPSFQGG
TPLDPMAAVAALPDEAEICGCNGVCKGTITAAIAAKGLTSLDDVRAHTKASASCGSCTGLVEQIMQISLGDSYTAAAVQP
ICSCTTLGHDDVRRLIKAKSLKTIPQVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERIHANIQKDGT
YSVVPRMWGGVTSSSELRAIADVVDKFAIPAVKVTGGQRIDMLGIKKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVG
TDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGYEIHFAGAAGLDIKGTEVLGLV
KSEDEALEHIVALTQMYREQGRYLERIYKWAKRIGLAEIRQQIMEDHDKRRAYYDRFVFSQKFAQVDPWSERVSGKDKHE
FRPMAELDITQAAE

Sequences:

>Translated_814_residues
MQKLVVIGAGMASGRVLEHLFETDPDAYQVTLFNAEPRGNYNRIMLSPVLSGEKSYEEIVIHDDAWYVRHNVICRFGERV
LEVDRARKVVVGPKGEIAYDKLLIATGSVPFIVPVDGHKLPGVISYRDLDDTNAMVEAAGVPGARAVVIGGGLLGLEAAA
GLRLRGMEVTVVHLAGHLMERQLDEAAGYLLSKDLERRGIAVITRASTKAIVGTERVEGVQLEDGTLLPADLVVMAVGIR
PAADLAKQSGLDVGRAVRVNAQMQTSDPDIYAVGECVEFEGTLFGLVAPLYDQAKVVAKSLLGEADAFAVKELSTKLKVT
GCDLFSAGSFADGAGREDIVLRDPARGIYKRLVIEDDRLVGAVMYGDTSDSNWFFGLIRDGVKIADMRDTLIFGPSFQGG
TPLDPMAAVAALPDEAEICGCNGVCKGTITAAIAAKGLTSLDDVRAHTKASASCGSCTGLVEQIMQISLGDSYTAAAVQP
ICSCTTLGHDDVRRLIKAKSLKTIPQVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERIHANIQKDGT
YSVVPRMWGGVTSSSELRAIADVVDKFAIPAVKVTGGQRIDMLGIKKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVG
TDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGYEIHFAGAAGLDIKGTEVLGLV
KSEDEALEHIVALTQMYREQGRYLERIYKWAKRIGLAEIRQQIMEDHDKRRAYYDRFVFSQKFAQVDPWSERVSGKDKHE
FRPMAELDITQAAE
>Mature_814_residues
MQKLVVIGAGMASGRVLEHLFETDPDAYQVTLFNAEPRGNYNRIMLSPVLSGEKSYEEIVIHDDAWYVRHNVICRFGERV
LEVDRARKVVVGPKGEIAYDKLLIATGSVPFIVPVDGHKLPGVISYRDLDDTNAMVEAAGVPGARAVVIGGGLLGLEAAA
GLRLRGMEVTVVHLAGHLMERQLDEAAGYLLSKDLERRGIAVITRASTKAIVGTERVEGVQLEDGTLLPADLVVMAVGIR
PAADLAKQSGLDVGRAVRVNAQMQTSDPDIYAVGECVEFEGTLFGLVAPLYDQAKVVAKSLLGEADAFAVKELSTKLKVT
GCDLFSAGSFADGAGREDIVLRDPARGIYKRLVIEDDRLVGAVMYGDTSDSNWFFGLIRDGVKIADMRDTLIFGPSFQGG
TPLDPMAAVAALPDEAEICGCNGVCKGTITAAIAAKGLTSLDDVRAHTKASASCGSCTGLVEQIMQISLGDSYTAAAVQP
ICSCTTLGHDDVRRLIKAKSLKTIPQVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERIHANIQKDGT
YSVVPRMWGGVTSSSELRAIADVVDKFAIPAVKVTGGQRIDMLGIKKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVG
TDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGYEIHFAGAAGLDIKGTEVLGLV
KSEDEALEHIVALTQMYREQGRYLERIYKWAKRIGLAEIRQQIMEDHDKRRAYYDRFVFSQKFAQVDPWSERVSGKDKHE
FRPMAELDITQAAE

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI65787454, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI226437568, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=3e-21,
Organism=Escherichia coli, GI1789765, Length=809, Percent_Identity=35.5995055624227, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1789065, Length=379, Percent_Identity=26.3852242744063, Blast_Score=127, Evalue=4e-30,
Organism=Escherichia coli, GI1788892, Length=248, Percent_Identity=27.8225806451613, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=412, Percent_Identity=24.2718446601942, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281359715, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281359713, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639250, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18543267, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639252, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639257, Length=279, Percent_Identity=27.2401433691756, Blast_Score=91, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24581020, Length=242, Percent_Identity=30.1652892561983, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28573993, Length=242, Percent_Identity=30.1652892561983, Blast_Score=80, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585130, Length=279, Percent_Identity=23.2974910394265, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88447; Mature: 88447

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLVVIGAGMASGRVLEHLFETDPDAYQVTLFNAEPRGNYNRIMLSPVLSGEKSYEEIV
CCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCEEE
IHDDAWYVRHNVICRFGERVLEVDRARKVVVGPKGEIAYDKLLIATGSVPFIVPVDGHKL
EECCCEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCCCEEEECCCCCC
PGVISYRDLDDTNAMVEAAGVPGARAVVIGGGLLGLEAAAGLRLRGMEVTVVHLAGHLME
CCEEEECCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHCCCEEECCEEEEHHHHHHHHH
RQLDEAAGYLLSKDLERRGIAVITRASTKAIVGTERVEGVQLEDGTLLPADLVVMAVGIR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCCEEECCCCCCHHHHHHHEECCC
PAADLAKQSGLDVGRAVRVNAQMQTSDPDIYAVGECVEFEGTLFGLVAPLYDQAKVVAKS
CHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHH
LLGEADAFAVKELSTKLKVTGCDLFSAGSFADGAGREDIVLRDPARGIYKRLVIEDDRLV
HHCCCHHHHHHHHCCEEEEEECCEECCCCCCCCCCCCCEEEECCHHHHHHHHEEECCCEE
GAVMYGDTSDSNWFFGLIRDGVKIADMRDTLIFGPSFQGGTPLDPMAAVAALPDEAEICG
EEEEECCCCCCCEEEEEECCCEEEEECCCEEEECCCCCCCCCCCHHHHHHCCCCCCCEEC
CNGVCKGTITAAIAAKGLTSLDDVRAHTKASASCGSCTGLVEQIMQISLGDSYTAAAVQP
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ICSCTTLGHDDVRRLIKAKSLKTIPQVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYAD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCHHHHH
DYQSRFVNERIHANIQKDGTYSVVPRMWGGVTSSSELRAIADVVDKFAIPAVKVTGGQRI
HHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEE
DMLGIKKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVGTDWCRFGTQDSTGLGIRIEK
EEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEE
FMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGYEIHFAGAAGLDIKGTEVLGLV
EECCCCCHHHEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEEEEEE
KSEDEALEHIVALTQMYREQGRYLERIYKWAKRIGLAEIRQQIMEDHDKRRAYYDRFVFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QKFAQVDPWSERVSGKDKHEFRPMAELDITQAAE
HHHHCCCCHHHHCCCCCCHHCCCCHHCCCCCCCC
>Mature Secondary Structure
MQKLVVIGAGMASGRVLEHLFETDPDAYQVTLFNAEPRGNYNRIMLSPVLSGEKSYEEIV
CCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCEEE
IHDDAWYVRHNVICRFGERVLEVDRARKVVVGPKGEIAYDKLLIATGSVPFIVPVDGHKL
EECCCEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCCCEEEECCCCCC
PGVISYRDLDDTNAMVEAAGVPGARAVVIGGGLLGLEAAAGLRLRGMEVTVVHLAGHLME
CCEEEECCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHCCCEEECCEEEEHHHHHHHHH
RQLDEAAGYLLSKDLERRGIAVITRASTKAIVGTERVEGVQLEDGTLLPADLVVMAVGIR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCCEEECCCCCCHHHHHHHEECCC
PAADLAKQSGLDVGRAVRVNAQMQTSDPDIYAVGECVEFEGTLFGLVAPLYDQAKVVAKS
CHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHH
LLGEADAFAVKELSTKLKVTGCDLFSAGSFADGAGREDIVLRDPARGIYKRLVIEDDRLV
HHCCCHHHHHHHHCCEEEEEECCEECCCCCCCCCCCCCEEEECCHHHHHHHHEEECCCEE
GAVMYGDTSDSNWFFGLIRDGVKIADMRDTLIFGPSFQGGTPLDPMAAVAALPDEAEICG
EEEEECCCCCCCEEEEEECCCEEEEECCCEEEECCCCCCCCCCCHHHHHHCCCCCCCEEC
CNGVCKGTITAAIAAKGLTSLDDVRAHTKASASCGSCTGLVEQIMQISLGDSYTAAAVQP
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ICSCTTLGHDDVRRLIKAKSLKTIPQVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYAD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCHHHHH
DYQSRFVNERIHANIQKDGTYSVVPRMWGGVTSSSELRAIADVVDKFAIPAVKVTGGQRI
HHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEE
DMLGIKKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVGTDWCRFGTQDSTGLGIRIEK
EEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEE
FMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGYEIHFAGAAGLDIKGTEVLGLV
EECCCCCHHHEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEEEEEE
KSEDEALEHIVALTQMYREQGRYLERIYKWAKRIGLAEIRQQIMEDHDKRRAYYDRFVFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QKFAQVDPWSERVSGKDKHEFRPMAELDITQAAE
HHHHCCCCHHHHCCCCCCHHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]