Click here to switch to the map view.

The map label for this gene is braC [H]

Identifier: 154245000

GI number: 154245000

Start: 1203457

End: 1204602

Strand: Direct

Name: braC [H]

Synonym: Xaut_1050

Alternate gene names: 154245000

Gene position: 1203457-1204602 (Clockwise)

Preceding gene: 154244999

Following gene: 154245001

Centisome position: 22.67

GC content: 65.71

Gene sequence:

>1146_bases
ATGCTGCCCCTGAGACCCACCATCGCCGCATTGCTTATCGCCGCTGGCCTCTCGGCCGCCCACGCCGCCGACCCCATCAA
GATCGGCGGCCTTCTGGAGACCTCCGGCGCCGTCGCCTCCCTCGGCCAGCCTGGCCTTGAGGGCGCCATGCTGGCGGTGG
AGCAGATCAACAAGACGGGCGGCATCAACGGACAGCCCGTCGAGTTCGTGAACGTCAACACCGAGAGCGACAACACCCGC
GCCGTGACCGCCGTGAAGCGCCTCATCGGACAGGGCGACATCGCTGCCATCGTCGGGCCGATGAACTCCGGCTCGTCCTT
CGCCATCATCGACACCGTGCAGCGGGCCTCCGTGCCCATGATCTCCAATGGTGGCTCGCGCAACATCGTGCTGCCGGCCG
AGGAGAAGAAGTGGATCTTCCTCTCGCCCCTCACTGACGTGCTCGTGCAGAGCGTGATGATGGAGGACATGAAGAAGCGC
GGGATCACACGCATCGCCCTCCTGAATGCGGACAGTCCCTTCGGCACCAGCGGCCGCGAGCAACTGGAGAAGAACGCTGC
CAGCTACGGCATCACCGTCGTGTCCCAGCAGGCCTTCGGCAACGCCGATCAGGACATGACGCCCCAGCTCACCAAGGTGC
GCGGCACCGATGCGCAGGCGGTGGTGGTGTGGGCCACAGGCCCCGGACAGGCCATCGTGGTGAAGAATTATCGCCAGCTC
GGCATCGACCTGCCGCTCTATCTTTCCCAGGCCGCGAACGACTTCAACTTCCTGCGCCTCGCCGGCCCGGCTGCGGATGG
AATCTTCATCCCCTCCTCGAAGATCTATGTCACCGGCGCGCTGGCCGCGAACGACCCGCAGAAAGCCGTGATCGAGACCT
TCGCGAAGGATTACGAGGCCAAATACGGCAAGCCCCCGGCGACGTTCGCGGGTAACGCCTACGACGCGGTGATGATGCTG
GCGGAGGCGGTGAAGAAGGCCGGTGCCACCGATCGCGCCGCCATCCGCGGTGCCATCGAGGGCCTGAAAGACCACGTGGG
TGTCACCGCCGTCTACACCTACGGCCCCGGCGACCATTTCGGCGCCAAGGCGGACAGCGTGGTGATGCTCACCGTCAAGG
ACGGCAAGTTCGCCCTCGTGCGCTGA

Upstream 100 bases:

>100_bases
ATACCCCACCCCCGGCGCTGTTCGCCGAAGCCTTCAACGCCATCGCCTGACCCGAATTCGTTCCTGATCCGCCCACACAG
CCCCGACCGAAGGTGTTCCC

Downstream 100 bases:

>100_bases
TGACATTGCGGCGGCGCTGACCGCCGCGACCCGATCCATTCCCTTCACCCGATTCCGTTCGGCGATCCAGCCGGGCGCGG
AGACGCGCGCGCCCGGCCGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLPLRPTIAALLIAAGLSAAHAADPIKIGGLLETSGAVASLGQPGLEGAMLAVEQINKTGGINGQPVEFVNVNTESDNTR
AVTAVKRLIGQGDIAAIVGPMNSGSSFAIIDTVQRASVPMISNGGSRNIVLPAEEKKWIFLSPLTDVLVQSVMMEDMKKR
GITRIALLNADSPFGTSGREQLEKNAASYGITVVSQQAFGNADQDMTPQLTKVRGTDAQAVVVWATGPGQAIVVKNYRQL
GIDLPLYLSQAANDFNFLRLAGPAADGIFIPSSKIYVTGALAANDPQKAVIETFAKDYEAKYGKPPATFAGNAYDAVMML
AEAVKKAGATDRAAIRGAIEGLKDHVGVTAVYTYGPGDHFGAKADSVVMLTVKDGKFALVR

Sequences:

>Translated_381_residues
MLPLRPTIAALLIAAGLSAAHAADPIKIGGLLETSGAVASLGQPGLEGAMLAVEQINKTGGINGQPVEFVNVNTESDNTR
AVTAVKRLIGQGDIAAIVGPMNSGSSFAIIDTVQRASVPMISNGGSRNIVLPAEEKKWIFLSPLTDVLVQSVMMEDMKKR
GITRIALLNADSPFGTSGREQLEKNAASYGITVVSQQAFGNADQDMTPQLTKVRGTDAQAVVVWATGPGQAIVVKNYRQL
GIDLPLYLSQAANDFNFLRLAGPAADGIFIPSSKIYVTGALAANDPQKAVIETFAKDYEAKYGKPPATFAGNAYDAVMML
AEAVKKAGATDRAAIRGAIEGLKDHVGVTAVYTYGPGDHFGAKADSVVMLTVKDGKFALVR
>Mature_381_residues
MLPLRPTIAALLIAAGLSAAHAADPIKIGGLLETSGAVASLGQPGLEGAMLAVEQINKTGGINGQPVEFVNVNTESDNTR
AVTAVKRLIGQGDIAAIVGPMNSGSSFAIIDTVQRASVPMISNGGSRNIVLPAEEKKWIFLSPLTDVLVQSVMMEDMKKR
GITRIALLNADSPFGTSGREQLEKNAASYGITVVSQQAFGNADQDMTPQLTKVRGTDAQAVVVWATGPGQAIVVKNYRQL
GIDLPLYLSQAANDFNFLRLAGPAADGIFIPSSKIYVTGALAANDPQKAVIETFAKDYEAKYGKPPATFAGNAYDAVMML
AEAVKKAGATDRAAIRGAIEGLKDHVGVTAVYTYGPGDHFGAKADSVVMLTVKDGKFALVR

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=334, Percent_Identity=21.8562874251497, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39842; Mature: 39842

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLRPTIAALLIAAGLSAAHAADPIKIGGLLETSGAVASLGQPGLEGAMLAVEQINKTG
CCCCCHHHHHHHHHHCCCHHHCCCCEEECCEEECCCCHHHCCCCCCCHHHHHHHHHHHCC
GINGQPVEFVNVNTESDNTRAVTAVKRLIGQGDIAAIVGPMNSGSSFAIIDTVQRASVPM
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEHHHCCCCE
ISNGGSRNIVLPAEEKKWIFLSPLTDVLVQSVMMEDMKKRGITRIALLNADSPFGTSGRE
EECCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
QLEKNAASYGITVVSQQAFGNADQDMTPQLTKVRGTDAQAVVVWATGPGQAIVVKNYRQL
HHHHHHHHCCEEEEEHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCEEEEECHHHH
GIDLPLYLSQAANDFNFLRLAGPAADGIFIPSSKIYVTGALAANDPQKAVIETFAKDYEA
CCCCCHHHHHCCCCCCEEEEECCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHH
KYGKPPATFAGNAYDAVMMLAEAVKKAGATDRAAIRGAIEGLKDHVGVTAVYTYGPGDHF
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GAKADSVVMLTVKDGKFALVR
CCCCCCEEEEEEECCCEEEEC
>Mature Secondary Structure
MLPLRPTIAALLIAAGLSAAHAADPIKIGGLLETSGAVASLGQPGLEGAMLAVEQINKTG
CCCCCHHHHHHHHHHCCCHHHCCCCEEECCEEECCCCHHHCCCCCCCHHHHHHHHHHHCC
GINGQPVEFVNVNTESDNTRAVTAVKRLIGQGDIAAIVGPMNSGSSFAIIDTVQRASVPM
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEHHHCCCCE
ISNGGSRNIVLPAEEKKWIFLSPLTDVLVQSVMMEDMKKRGITRIALLNADSPFGTSGRE
EECCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
QLEKNAASYGITVVSQQAFGNADQDMTPQLTKVRGTDAQAVVVWATGPGQAIVVKNYRQL
HHHHHHHHCCEEEEEHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCEEEEECHHHH
GIDLPLYLSQAANDFNFLRLAGPAADGIFIPSSKIYVTGALAANDPQKAVIETFAKDYEA
CCCCCHHHHHCCCCCCEEEEECCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHH
KYGKPPATFAGNAYDAVMMLAEAVKKAGATDRAAIRGAIEGLKDHVGVTAVYTYGPGDHF
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GAKADSVVMLTVKDGKFALVR
CCCCCCEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]