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The map label for this gene is proP [H]

Identifier: 154244880

GI number: 154244880

Start: 1049131

End: 1050477

Strand: Reverse

Name: proP [H]

Synonym: Xaut_0930

Alternate gene names: 154244880

Gene position: 1050477-1049131 (Counterclockwise)

Preceding gene: 154244881

Following gene: 154244879

Centisome position: 19.79

GC content: 65.92

Gene sequence:

>1347_bases
ATGACGCACGCCACCTATCCCATTTCAGGACACACCGCGCCCGGCCTCAACAAAGGACAATGGCGCATCATCTTCCTTGC
GAGCCTCGGCGGCTCCCTTGAGTTCTACGACTTCATTATTTACGGCATCTTTGTCCAGTACATCTCGGCACAGTTCTTTC
CGGCCGACGACCCCTATGTCTCCCTGATCCTGTCCTTCTCGGTGCTGGCCCTCGGCTTCCTGGCACGGCCGTTCGGTGGC
GCGCTGCTCGGCACGCTGGGTGATCGGTATGGACGCAGGCCGGTCTTCATCGCCTCCCTTGGCCTGACCACGCTTGCAAC
CATCGCCATCGGCCTCCTTCCCAACTACCAGTCATGGGGCATCGCCGCGCCGGTGCTGCTGGTGGCCTTCCGTCTCGTGC
AGGGCATCTGCCTCGGCGGCGAGCTGCCGGGTGCGGTCACCTATGCGGTGGAGGCAGCGCCACGGCGCGCCGGCCTCGCG
TGCGGTTTCATCATCTTCTGCGTCAATGTCGGCGTGCTCATCGCCACCCTCGTCAACCTCGGCATCCAGCATGCGCTTCC
GCCTCAGGACGTTGCCGAATACGGCTGGCGCATCGCCTTCCTGCTCGGCGGCGTCATCGGCCTGATCAGCTTTGCCCTGC
GCCGCAAGCTGGAGGAATCGCCCGAGTTCGTGAACCTCCACGACAAGGCTGCGAGGCATCCGCTGGGAGAACTGATGCGC
AATCACGGCCGTTCGGTGCTGGTCGCGGCCGGTGTCGCCGCCATCATCGCCGGCTTCAACGGCATGCTCTACGGCTTCGT
GCCCGCCTTCCTGGTGAAGACGCTGCACTATCCGGCCCCCGATGTGGCGCTGGCCATGACGGTCGCGCTGGTGGTCAGCT
CCGCCGGCCTCATCATTGCGGGATGGGCCGGCGATTTCGTGCCCGGACGCCTCATCCTGCGCGCCGGTTCGCTGACCATG
CTCATCGTGGTGGTTCCGCTGTTCGCGCTCATGGCGAACCACGCGATCAGCATCGTGCTGGTGCTGAGCCTGCTGTCGCT
GGTGTTCTCTCTGGCGAGCGGCATCTGGCCCAGCATCCTCGCCGGCCTGTTCCCGACCCAGGTCCGCTTCACCGGCCTTG
CCCTGTCATACAACATGAGCGTCACCCTCCTCTCGGGCTTCGCGCCGCTCGCCGCGACGATACTCATCGAGCAGACGCGG
ATGGACGCAGCGCCGGCGCTCTACATCGCCCTGTGCGCGGCCCTGAGCTTCTGGTGCAGCTTCCTCGTCCCCGGACGGGC
ACGCGCGGCGGCGGTTCCGGAAGCCACGCGGGCAAGCCTCGATGCCTCGGACATGCCACCGGCCTGA

Upstream 100 bases:

>100_bases
GCCCGCTTTGCCTTGCGCTGAAGCGGAGGGGACCTACGGCGCGGCGTGCATCTTGTTGCGCGAACACAGCCAATAAATAA
TATGGAGGCCCCGAGGATCA

Downstream 100 bases:

>100_bases
GACCGTCCACGAGACGATTGCGGGCGGCCGGCGTACACGGCCTCCCACCGCCCATCACAACGCCCCTGACACAGCGCCCA
CCCCAACACAGAGGAACAAC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTHATYPISGHTAPGLNKGQWRIIFLASLGGSLEFYDFIIYGIFVQYISAQFFPADDPYVSLILSFSVLALGFLARPFGG
ALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPNYQSWGIAAPVLLVAFRLVQGICLGGELPGAVTYAVEAAPRRAGLA
CGFIIFCVNVGVLIATLVNLGIQHALPPQDVAEYGWRIAFLLGGVIGLISFALRRKLEESPEFVNLHDKAARHPLGELMR
NHGRSVLVAAGVAAIIAGFNGMLYGFVPAFLVKTLHYPAPDVALAMTVALVVSSAGLIIAGWAGDFVPGRLILRAGSLTM
LIVVVPLFALMANHAISIVLVLSLLSLVFSLASGIWPSILAGLFPTQVRFTGLALSYNMSVTLLSGFAPLAATILIEQTR
MDAAPALYIALCAALSFWCSFLVPGRARAAAVPEATRASLDASDMPPA

Sequences:

>Translated_448_residues
MTHATYPISGHTAPGLNKGQWRIIFLASLGGSLEFYDFIIYGIFVQYISAQFFPADDPYVSLILSFSVLALGFLARPFGG
ALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPNYQSWGIAAPVLLVAFRLVQGICLGGELPGAVTYAVEAAPRRAGLA
CGFIIFCVNVGVLIATLVNLGIQHALPPQDVAEYGWRIAFLLGGVIGLISFALRRKLEESPEFVNLHDKAARHPLGELMR
NHGRSVLVAAGVAAIIAGFNGMLYGFVPAFLVKTLHYPAPDVALAMTVALVVSSAGLIIAGWAGDFVPGRLILRAGSLTM
LIVVVPLFALMANHAISIVLVLSLLSLVFSLASGIWPSILAGLFPTQVRFTGLALSYNMSVTLLSGFAPLAATILIEQTR
MDAAPALYIALCAALSFWCSFLVPGRARAAAVPEATRASLDASDMPPA
>Mature_447_residues
THATYPISGHTAPGLNKGQWRIIFLASLGGSLEFYDFIIYGIFVQYISAQFFPADDPYVSLILSFSVLALGFLARPFGGA
LLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPNYQSWGIAAPVLLVAFRLVQGICLGGELPGAVTYAVEAAPRRAGLAC
GFIIFCVNVGVLIATLVNLGIQHALPPQDVAEYGWRIAFLLGGVIGLISFALRRKLEESPEFVNLHDKAARHPLGELMRN
HGRSVLVAAGVAAIIAGFNGMLYGFVPAFLVKTLHYPAPDVALAMTVALVVSSAGLIIAGWAGDFVPGRLILRAGSLTML
IVVVPLFALMANHAISIVLVLSLLSLVFSLASGIWPSILAGLFPTQVRFTGLALSYNMSVTLLSGFAPLAATILIEQTRM
DAAPALYIALCAALSFWCSFLVPGRARAAAVPEATRASLDASDMPPA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=29.03981264637, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=393, Percent_Identity=28.2442748091603, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788942, Length=424, Percent_Identity=30.6603773584906, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789941, Length=412, Percent_Identity=31.5533980582524, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082231, Length=495, Percent_Identity=25.8585858585859, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87082404, Length=437, Percent_Identity=26.0869565217391, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47322; Mature: 47190

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHATYPISGHTAPGLNKGQWRIIFLASLGGSLEFYDFIIYGIFVQYISAQFFPADDPYV
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLILSFSVLALGFLARPFGGALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPNYQSWG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
IAAPVLLVAFRLVQGICLGGELPGAVTYAVEAAPRRAGLACGFIIFCVNVGVLIATLVNL
HHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIQHALPPQDVAEYGWRIAFLLGGVIGLISFALRRKLEESPEFVNLHDKAARHPLGELMR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHCCHHHHHHH
NHGRSVLVAAGVAAIIAGFNGMLYGFVPAFLVKTLHYPAPDVALAMTVALVVSSAGLIIA
HCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
GWAGDFVPGRLILRAGSLTMLIVVVPLFALMANHAISIVLVLSLLSLVFSLASGIWPSIL
ECCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AGLFPTQVRFTGLALSYNMSVTLLSGFAPLAATILIEQTRMDAAPALYIALCAALSFWCS
HHHCCCHHEEEEEEEEECCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FLVPGRARAAAVPEATRASLDASDMPPA
HHCCCCCHHCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
THATYPISGHTAPGLNKGQWRIIFLASLGGSLEFYDFIIYGIFVQYISAQFFPADDPYV
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLILSFSVLALGFLARPFGGALLGTLGDRYGRRPVFIASLGLTTLATIAIGLLPNYQSWG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
IAAPVLLVAFRLVQGICLGGELPGAVTYAVEAAPRRAGLACGFIIFCVNVGVLIATLVNL
HHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIQHALPPQDVAEYGWRIAFLLGGVIGLISFALRRKLEESPEFVNLHDKAARHPLGELMR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHCCHHHHHHH
NHGRSVLVAAGVAAIIAGFNGMLYGFVPAFLVKTLHYPAPDVALAMTVALVVSSAGLIIA
HCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
GWAGDFVPGRLILRAGSLTMLIVVVPLFALMANHAISIVLVLSLLSLVFSLASGIWPSIL
ECCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AGLFPTQVRFTGLALSYNMSVTLLSGFAPLAATILIEQTRMDAAPALYIALCAALSFWCS
HHHCCCHHEEEEEEEEECCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FLVPGRARAAAVPEATRASLDASDMPPA
HHCCCCCHHCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]