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The map label for this gene is paaI [H]

Identifier: 154244852

GI number: 154244852

Start: 1004672

End: 1005169

Strand: Reverse

Name: paaI [H]

Synonym: Xaut_0902

Alternate gene names: 154244852

Gene position: 1005169-1004672 (Counterclockwise)

Preceding gene: 154244853

Following gene: 154244851

Centisome position: 18.93

GC content: 69.48

Gene sequence:

>498_bases
ATGAACACCCACGCCCCTCATCCCGTCCCGCCGCGCACGCCACAGGACACCGCGCGTCTAAGCGCGGAGGCCATGTGGGC
CACCGACCATGCGAGCCAGGGCCTCGGGATGGAGCTGGTGGCGGTGGGCCCCGGCACGGCGACGCTCTCCATGACGCTCA
CCGAGCGAATGTGCAACGGCTTCGGCATGGGCCATGGCGGCTTCATCTTCGCCCTTGCAGACAGCGCCTTCGCCTTTGCC
TGCAACTCCTATGACGAGCGCACCGTGGCGGCCCATTGCGCGGTCACCTACCTGCGCCCCGGCACCCGCGGAAAGACACT
CGTCGCGACGGCGCGGGAGATCGCCCGCACAGGACGCTCGGGCATCTACGACGTGGAGGTGAGCGAGGACGGCGTGGTGA
TCGCGCAGTTTCGCGGGCATTCTCGCACCATCGGCGGGGCGTTGATCGCCCCGCCCCCAAGCGCGCCGGAGAGCCGCGAC
CACACGACCACAGCTTGA

Upstream 100 bases:

>100_bases
AGCGCGACCTCCAGCGCGAGGCCGGCCGCACGCCGGACTATCGCGAAGGCGTTTCCGCCTTCATGGAAAAGCGCCCGGCG
CGCTTCTCGGGGCGGCGCGC

Downstream 100 bases:

>100_bases
GTGGAGGAGACGATGAACATGGCTCTGCCGGCGGTTTCCTTTGGCCGGACCTTCGAGGCCGGCCTTGATACAGCGGAACG
GGCATCGCGGGACGAAATCA

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNTHAPHPVPPRTPQDTARLSAEAMWATDHASQGLGMELVAVGPGTATLSMTLTERMCNGFGMGHGGFIFALADSAFAFA
CNSYDERTVAAHCAVTYLRPGTRGKTLVATAREIARTGRSGIYDVEVSEDGVVIAQFRGHSRTIGGALIAPPPSAPESRD
HTTTA

Sequences:

>Translated_165_residues
MNTHAPHPVPPRTPQDTARLSAEAMWATDHASQGLGMELVAVGPGTATLSMTLTERMCNGFGMGHGGFIFALADSAFAFA
CNSYDERTVAAHCAVTYLRPGTRGKTLVATAREIARTGRSGIYDVEVSEDGVVIAQFRGHSRTIGGALIAPPPSAPESRD
HTTTA
>Mature_165_residues
MNTHAPHPVPPRTPQDTARLSAEAMWATDHASQGLGMELVAVGPGTATLSMTLTERMCNGFGMGHGGFIFALADSAFAFA
CNSYDERTVAAHCAVTYLRPGTRGKTLVATAREIARTGRSGIYDVEVSEDGVVIAQFRGHSRTIGGALIAPPPSAPESRD
HTTTA

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=129, Percent_Identity=41.8604651162791, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 17325; Mature: 17325

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHAPHPVPPRTPQDTARLSAEAMWATDHASQGLGMELVAVGPGTATLSMTLTERMCNG
CCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHH
FGMGHGGFIFALADSAFAFACNSYDERTVAAHCAVTYLRPGTRGKTLVATAREIARTGRS
CCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCC
GIYDVEVSEDGVVIAQFRGHSRTIGGALIAPPPSAPESRDHTTTA
CEEEEEECCCCEEEEEECCCCEECCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTHAPHPVPPRTPQDTARLSAEAMWATDHASQGLGMELVAVGPGTATLSMTLTERMCNG
CCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHH
FGMGHGGFIFALADSAFAFACNSYDERTVAAHCAVTYLRPGTRGKTLVATAREIARTGRS
CCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCC
GIYDVEVSEDGVVIAQFRGHSRTIGGALIAPPPSAPESRDHTTTA
CEEEEEECCCCEEEEEECCCCEECCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]