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The map label for this gene is braC [H]

Identifier: 154244843

GI number: 154244843

Start: 995886

End: 997076

Strand: Reverse

Name: braC [H]

Synonym: Xaut_0893

Alternate gene names: 154244843

Gene position: 997076-995886 (Counterclockwise)

Preceding gene: 154244851

Following gene: 154244842

Centisome position: 18.78

GC content: 68.09

Gene sequence:

>1191_bases
ATGGATACGACCGCTCGCCTGCGGCCGGTGCTGCGCGCCGCCCTCGCCTTCGCAAGCTTCGCCCTCGCCCCCTTCGCGCC
GCTGGCCGCCGCGCCCGCCCTGGCCGCAGACCCCATCAAGATCGGTTCGGTCGTGTCCGCCACCGGCCCGTCCGCCTTCC
TCGGCGACCCCGAGGCCAAGACCCTGAAGCTCTATGTGGAGGAGCTGAACAAGAAGGGCGGGCTGCTCGGCCGCCCGGTG
GAGCTGGTGCTCTACGACGACGGCGGCGACGCCAACAAGGCGCGCACCTTCGCCACCCGTCTGGTGGAAGACGACAAGGT
GGTGGCCATGGTGGGCGGCACCTCCACCGGCGCCACCATGGCCATGATCCCGGTGTTCGAGGAGGCCGGCATCCCCTTCG
TGTCGCTGGCCGGCGGCATCGAGATCATCGAGCCGGTGCGCGCCTTCGTCTTCAAGACGCCCCACACCGACAAGACCGCC
TGCCAGAAGATCTTCGAGGACATGAAGGCACGCGGCATCACGAAGATCGGCATGATCTCGGGCACCGACGGCTTCGGCAA
GTCCATGCGCAACCAATGCCTGAAGGTGGTGGGCGGCTACAACATCACCGTGCTCGCCGACGAGACCTACGGCGCCACCG
ATTCCGACATGACGCCCCAGCTCAACAAGATCAAGAACACTGCCGGCGTCGAGGCGGTGCTCAATCCCGGCTTCGGCCAG
GGACCGGCCATCGTGACCCGCAACTATGCCCAGCTCGGCCTCAAGCTGCCGCTGTACCAGAGCCACGGCGTCGCCTCCAA
GAGCTTCATCGAGCTGGCGGGACCGGCGGCGGAAGGCGTGCGGCTGCCTGCCCCCGCCATCCTCGTTGGCGACAAGCTCG
CGGCGGAGGACCCCCAGAAAGCGGTGGTCGCCGGCTACAAGGCGTCCTACGAGAAGGCCACGGGCCAGAGCGTCTCCACC
TTCGGCGGCTACGGCTTCGACGGCATCCAGCTGGTGACGCAGGCCATCGACAAGGCCAAGTCCGCCGAGCCGAAGAAGAT
CCGCGACGCGCTGGAAGCCACCAAGTCCTATGTGGGCGTGACCGGCGTCTACAGCTTCTCGCCCACCGACCACCTGGGCC
TCGGCACGGACTCGTTCCGCATGCTGGAGATCCGTGGCGGCGACTGGACGCTGGACGCCGCCACGCGCTGA

Upstream 100 bases:

>100_bases
TCAATTATATAGTTCCCCTACCGTCGGCTCAATGGCGCGGCGGCTGGAGGCGGGCAAACCCGCTCCTTGGCCCGAAACGG
ACCTTTTGGAGAAACGCTTC

Downstream 100 bases:

>100_bases
GGCGCCCCTTAACCCCTGCCCCCGAAACAGGGCGGCGCCGCGCGCGCCGTCCGAGCGATGGCGCTAAAGGCCAGGCCCGA
CCATGTCCGAATTTCTCCAG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDTTARLRPVLRAALAFASFALAPFAPLAAAPALAADPIKIGSVVSATGPSAFLGDPEAKTLKLYVEELNKKGGLLGRPV
ELVLYDDGGDANKARTFATRLVEDDKVVAMVGGTSTGATMAMIPVFEEAGIPFVSLAGGIEIIEPVRAFVFKTPHTDKTA
CQKIFEDMKARGITKIGMISGTDGFGKSMRNQCLKVVGGYNITVLADETYGATDSDMTPQLNKIKNTAGVEAVLNPGFGQ
GPAIVTRNYAQLGLKLPLYQSHGVASKSFIELAGPAAEGVRLPAPAILVGDKLAAEDPQKAVVAGYKASYEKATGQSVST
FGGYGFDGIQLVTQAIDKAKSAEPKKIRDALEATKSYVGVTGVYSFSPTDHLGLGTDSFRMLEIRGGDWTLDAATR

Sequences:

>Translated_396_residues
MDTTARLRPVLRAALAFASFALAPFAPLAAAPALAADPIKIGSVVSATGPSAFLGDPEAKTLKLYVEELNKKGGLLGRPV
ELVLYDDGGDANKARTFATRLVEDDKVVAMVGGTSTGATMAMIPVFEEAGIPFVSLAGGIEIIEPVRAFVFKTPHTDKTA
CQKIFEDMKARGITKIGMISGTDGFGKSMRNQCLKVVGGYNITVLADETYGATDSDMTPQLNKIKNTAGVEAVLNPGFGQ
GPAIVTRNYAQLGLKLPLYQSHGVASKSFIELAGPAAEGVRLPAPAILVGDKLAAEDPQKAVVAGYKASYEKATGQSVST
FGGYGFDGIQLVTQAIDKAKSAEPKKIRDALEATKSYVGVTGVYSFSPTDHLGLGTDSFRMLEIRGGDWTLDAATR
>Mature_396_residues
MDTTARLRPVLRAALAFASFALAPFAPLAAAPALAADPIKIGSVVSATGPSAFLGDPEAKTLKLYVEELNKKGGLLGRPV
ELVLYDDGGDANKARTFATRLVEDDKVVAMVGGTSTGATMAMIPVFEEAGIPFVSLAGGIEIIEPVRAFVFKTPHTDKTA
CQKIFEDMKARGITKIGMISGTDGFGKSMRNQCLKVVGGYNITVLADETYGATDSDMTPQLNKIKNTAGVEAVLNPGFGQ
GPAIVTRNYAQLGLKLPLYQSHGVASKSFIELAGPAAEGVRLPAPAILVGDKLAAEDPQKAVVAGYKASYEKATGQSVST
FGGYGFDGIQLVTQAIDKAKSAEPKKIRDALEATKSYVGVTGVYSFSPTDHLGLGTDSFRMLEIRGGDWTLDAATR

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=339, Percent_Identity=24.188790560472, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994941, Length=312, Percent_Identity=24.6794871794872, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41513; Mature: 41513

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTARLRPVLRAALAFASFALAPFAPLAAAPALAADPIKIGSVVSATGPSAFLGDPEAK
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCEEECCCCCCCCCCCCHH
TLKLYVEELNKKGGLLGRPVELVLYDDGGDANKARTFATRLVEDDKVVAMVGGTSTGATM
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEE
AMIPVFEEAGIPFVSLAGGIEIIEPVRAFVFKTPHTDKTACQKIFEDMKARGITKIGMIS
EEEEEHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
GTDGFGKSMRNQCLKVVGGYNITVLADETYGATDSDMTPQLNKIKNTAGVEAVLNPGFGQ
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCC
GPAIVTRNYAQLGLKLPLYQSHGVASKSFIELAGPAAEGVRLPAPAILVGDKLAAEDPQK
CCEEEECCHHHHCEECCCCCCCCCCCHHHHEECCCCCCCCCCCCCEEEECCCCCCCCCCH
AVVAGYKASYEKATGQSVSTFGGYGFDGIQLVTQAIDKAKSAEPKKIRDALEATKSYVGV
HHHHCCHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCE
TGVYSFSPTDHLGLGTDSFRMLEIRGGDWTLDAATR
EEEEECCCCCCCCCCCCCEEEEEEECCCEEECCCCC
>Mature Secondary Structure
MDTTARLRPVLRAALAFASFALAPFAPLAAAPALAADPIKIGSVVSATGPSAFLGDPEAK
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCEEECCCCCCCCCCCCHH
TLKLYVEELNKKGGLLGRPVELVLYDDGGDANKARTFATRLVEDDKVVAMVGGTSTGATM
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEE
AMIPVFEEAGIPFVSLAGGIEIIEPVRAFVFKTPHTDKTACQKIFEDMKARGITKIGMIS
EEEEEHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
GTDGFGKSMRNQCLKVVGGYNITVLADETYGATDSDMTPQLNKIKNTAGVEAVLNPGFGQ
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCC
GPAIVTRNYAQLGLKLPLYQSHGVASKSFIELAGPAAEGVRLPAPAILVGDKLAAEDPQK
CCEEEECCHHHHCEECCCCCCCCCCCHHHHEECCCCCCCCCCCCCEEEECCCCCCCCCCH
AVVAGYKASYEKATGQSVSTFGGYGFDGIQLVTQAIDKAKSAEPKKIRDALEATKSYVGV
HHHHCCHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCE
TGVYSFSPTDHLGLGTDSFRMLEIRGGDWTLDAATR
EEEEECCCCCCCCCCCCCEEEEEEECCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]