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The map label for this gene is traG [H]

Identifier: 154244544

GI number: 154244544

Start: 660227

End: 662206

Strand: Direct

Name: traG [H]

Synonym: Xaut_0589

Alternate gene names: 154244544

Gene position: 660227-662206 (Clockwise)

Preceding gene: 154244543

Following gene: 154244545

Centisome position: 12.44

GC content: 66.21

Gene sequence:

>1980_bases
ATGTCCGCGACCAAGATCCTTTGGGGCCAGATCCTCCTTGTCCTCGCCATCGTGCTCGCCACCACCTGGGGCGCCACGCA
GGTCGTGGCCTGGCGGCTGGGCTTCCAGGACCAACTGGGACCGCCCTGGTTCCTGATGGGGCCCTGGGCCGTCTACCCTC
CGCCCGCTTTCTTCTGGTGGTGGTATTTTTACGACGCCTATGCGCCGGCCATCTTCGTTGAGGGGGCGATCATCGCCGCC
TCGGGTGGGTTCCTGTCCATCGCCGCCGCCATCGGGCTTTCGGTGTGGCGGGCGCGGGAAGCGAAGCGCGTCGAGACCTA
TGGCTCCGCCCGCTGGGCCGAGCCGGACGAGATCCGGTCAGCCGGACTGCTCGGGCCCGATGGCGTGGTGCTGGGGCGCC
ATGGTCGGGACTATCTGCGCCATGACGGACCGGAGCACGTTTTGTGCTTCGCACCGACCCGGTCGGGCAAGGGCGTCGGC
CTCGTCGTACCCTCGCTGCTCACCTGGCCCGGCTCGGCCATCGTCCACGACATCAAGGGCGAGAACTGGCAGCTCACCGC
AGGCTTCCGGGCGCGGCACGGGCGGGTGTTGCTGTTCGACCCCACCAATGTGCGGTCCTCCGCCTACAATCCCCTGCTGG
AGGTCCGCCGCGGGGAATGGGAGGTGCGGGACGTCCAGAACATTGCCGACATCCTGGTGGACCCGGAAGGCTCCCTGGAA
AAGCGCAACCACTGGGAGAAGACCAGCCACGCCCTCCTCGTGGGCGCCATCCTCCATGTGCTCTATGCCGAGCGGGACAA
GACCCTGGCCGGTGTCGCCGCCTTCCTCTCCGATCCCCGGCGGCCCATCGAGACGACGCTCGCGGCCATGATGACGACGG
CGCACCTCGGGGAGGCGGGTCCCCATCCGGTGATCGCCAGCGCCGCCCGCGAGCTGCTCAACAAGTCCGAGAACGAGCGG
TCCGGCGTGCTCTCCACCGCCATGTCCTTCCTCGGGCTTTATCGTGATCCCGTGGTCGCGGAGGTGACCCGATGCTGCGA
CTGGCGTATCACCGACCTTGTGGCAGGTGCCCGGCCGACCACACTCTACCTCGTCGTGCCGCCGTCTGACATCAACCGGA
CCAAGCCGCTTATTCGGCTGATCCTGAACCAGGTTGGTCGCCGCCTCACTGAGGACCTGCAGGCGAAGGCCGGTCGCCAC
CGGCTTCTCCTGATGCTGGACGAGTTCCCCGCGCTCGGGCGGCTCGACTTCTTCGAGAGCGCGCTCGCCTTCATGGCGGG
CTACGGGCTGAAGAGCTTCCTCATCGCCCAATCTCTGAACCAGATCGAGAAGGCCTACGGGCCGAACAACTCCATCCTCG
ACAACTGCCATGTGCGGGTGAGCTTCGCCACCAATGACGAGCGCACCGCCAAGCGCGTCTCCGATGCCCTCGGCACTGCC
ACCGAAATGAAGGCCATGAAGAACTATGCTGGCCACCGACTCTCCCCCTGGCTTGGTCACCTCATGGTCTCCCGCTCGGA
GACAGCCCGTCCGCTTCTGACGCCGGGCGAAGTGATGCAGCTTCCGCCCGCCGACGAGATCGTCATGGTGGCCGGCACGC
CGCCGATCCGGGCGAAGAAGGCGCGCTACTATGAGGATGAGCGGTTCAGGGAGCGGGTACTGCATCCGCCGGAGCTCTCG
GCTCCTTGCGATCGCCAGACCGACGACTGGACGGTACTTCCGTTGCCCGCGCCATCGCAACTGAGCCTGATGGTCGAGGA
AAGTGACGATGAGGACACAACCGGCTCGGAGCAACGGCGCCAGCCGGAGCTTGCACGAGTAGAGACGGTGGAGACGTCGC
AGCAAGTGCGGAACGAGTTCGAGATCGATCTGGTCGATGAGACTGAAGACGACGTGGCCCGGACCAGTCGCATGACGGGC
GTCATGCAGGGCGTCGCGCGTCAGGTCTCGCTCGATCCCAATGATGGCATGGAGCTGTGA

Upstream 100 bases:

>100_bases
AGCACATCTCCGGCGTCGCCCGCTCAGACGGCGGCATCGACTGGTCCTTCGGCCGCAAGCGCGGCCTCGGCCTCTAACTT
GCTAATCACTGGAGCACCGC

Downstream 100 bases:

>100_bases
TAGCGATGACCCCACGCAAGAAGAAGGCTCAGATCTCGGTCTATCTGGATCCCGCGACGATGGGGCTGCTGGCCGACTAT
GCCGCGCGGCGCGGCCAGCC

Product: conjugal transfer coupling protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSATKILWGQILLVLAIVLATTWGATQVVAWRLGFQDQLGPPWFLMGPWAVYPPPAFFWWWYFYDAYAPAIFVEGAIIAA
SGGFLSIAAAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGPDGVVLGRHGRDYLRHDGPEHVLCFAPTRSGKGVG
LVVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVRSSAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAAFLSDPRRPIETTLAAMMTTAHLGEAGPHPVIASAARELLNKSENER
SGVLSTAMSFLGLYRDPVVAEVTRCCDWRITDLVAGARPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRH
RLLLMLDEFPALGRLDFFESALAFMAGYGLKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDERFRERVLHPPELS
APCDRQTDDWTVLPLPAPSQLSLMVEESDDEDTTGSEQRRQPELARVETVETSQQVRNEFEIDLVDETEDDVARTSRMTG
VMQGVARQVSLDPNDGMEL

Sequences:

>Translated_659_residues
MSATKILWGQILLVLAIVLATTWGATQVVAWRLGFQDQLGPPWFLMGPWAVYPPPAFFWWWYFYDAYAPAIFVEGAIIAA
SGGFLSIAAAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGPDGVVLGRHGRDYLRHDGPEHVLCFAPTRSGKGVG
LVVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVRSSAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAAFLSDPRRPIETTLAAMMTTAHLGEAGPHPVIASAARELLNKSENER
SGVLSTAMSFLGLYRDPVVAEVTRCCDWRITDLVAGARPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRH
RLLLMLDEFPALGRLDFFESALAFMAGYGLKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDERFRERVLHPPELS
APCDRQTDDWTVLPLPAPSQLSLMVEESDDEDTTGSEQRRQPELARVETVETSQQVRNEFEIDLVDETEDDVARTSRMTG
VMQGVARQVSLDPNDGMEL
>Mature_658_residues
SATKILWGQILLVLAIVLATTWGATQVVAWRLGFQDQLGPPWFLMGPWAVYPPPAFFWWWYFYDAYAPAIFVEGAIIAAS
GGFLSIAAAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGPDGVVLGRHGRDYLRHDGPEHVLCFAPTRSGKGVGL
VVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVRSSAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLEK
RNHWEKTSHALLVGAILHVLYAERDKTLAGVAAFLSDPRRPIETTLAAMMTTAHLGEAGPHPVIASAARELLNKSENERS
GVLSTAMSFLGLYRDPVVAEVTRCCDWRITDLVAGARPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHR
LLLMLDEFPALGRLDFFESALAFMAGYGLKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTAT
EMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDERFRERVLHPPELSA
PCDRQTDDWTVLPLPAPSQLSLMVEESDDEDTTGSEQRRQPELARVETVETSQQVRNEFEIDLVDETEDDVARTSRMTGV
MQGVARQVSLDPNDGMEL

Specific function: Required for conjugative transfer of plasmid RP4. Binds tightly and specifically to the relaxase traI. Can also bind to DNA without sequence specificity. May form a pore-like structure that could serve as a channel for DNA transfer [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virD4/traG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003688 [H]

Pfam domain/function: PF02534 TraG [H]

EC number: NA

Molecular weight: Translated: 73176; Mature: 73045

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATKILWGQILLVLAIVLATTWGATQVVAWRLGFQDQLGPPWFLMGPWAVYPPPAFFWW
CCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHH
WYFYDAYAPAIFVEGAIIAASGGFLSIAAAIGLSVWRAREAKRVETYGSARWAEPDEIRS
HHHHHHHCCHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGLLGPDGVVLGRHGRDYLRHDGPEHVLCFAPTRSGKGVGLVVPSLLTWPGSAIVHDIKG
CCCCCCCCEEECCCCCHHHHCCCCCEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEECCC
ENWQLTAGFRARHGRVLLFDPTNVRSSAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
CCEEEEECCCCCCCEEEEECCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCCCH
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAAFLSDPRRPIETTLAAMMTTAHLGEAG
HHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PHPVIASAARELLNKSENERSGVLSTAMSFLGLYRDPVVAEVTRCCDWRITDLVAGARPT
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCC
TLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHRLLLMLDEFPALGRLDFFES
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
ALAFMAGYGLKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHH
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKK
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCHH
ARYYEDERFRERVLHPPELSAPCDRQTDDWTVLPLPAPSQLSLMVEESDDEDTTGSEQRR
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHHC
QPELARVETVETSQQVRNEFEIDLVDETEDDVARTSRMTGVMQGVARQVSLDPNDGMEL
CCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SATKILWGQILLVLAIVLATTWGATQVVAWRLGFQDQLGPPWFLMGPWAVYPPPAFFWW
CCCHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHH
WYFYDAYAPAIFVEGAIIAASGGFLSIAAAIGLSVWRAREAKRVETYGSARWAEPDEIRS
HHHHHHHCCHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGLLGPDGVVLGRHGRDYLRHDGPEHVLCFAPTRSGKGVGLVVPSLLTWPGSAIVHDIKG
CCCCCCCCEEECCCCCHHHHCCCCCEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEECCC
ENWQLTAGFRARHGRVLLFDPTNVRSSAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
CCEEEEECCCCCCCEEEEECCCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCCCH
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAAFLSDPRRPIETTLAAMMTTAHLGEAG
HHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PHPVIASAARELLNKSENERSGVLSTAMSFLGLYRDPVVAEVTRCCDWRITDLVAGARPT
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCCC
TLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHRLLLMLDEFPALGRLDFFES
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
ALAFMAGYGLKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHH
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKK
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCHH
ARYYEDERFRERVLHPPELSAPCDRQTDDWTVLPLPAPSQLSLMVEESDDEDTTGSEQRR
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHHC
QPELARVETVETSQQVRNEFEIDLVDETEDDVARTSRMTGVMQGVARQVSLDPNDGMEL
CCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1665997; 8021214; 11976307 [H]