Click here to switch to the map view.

The map label for this gene is mcbR [H]

Identifier: 154244388

GI number: 154244388

Start: 469078

End: 469827

Strand: Direct

Name: mcbR [H]

Synonym: Xaut_0431

Alternate gene names: 154244388

Gene position: 469078-469827 (Clockwise)

Preceding gene: 154244380

Following gene: 154244389

Centisome position: 8.84

GC content: 71.07

Gene sequence:

>750_bases
ATGGAACACAAGTCGGCATCTGCGGACGAGCGGGAGGACGGGGCAGCGGGAGAGGCGTCCGGAGGCCTCGACGCGCGCGT
CGGCCGCATCGTGCGCCAGACGCTGGCGGACCAGGTCTATGGCGACCTCAAGGAGATCCTGCTCTCCGGTCGCGCCGCCC
CGGGCGAGCGCTTCACCTTGCGCGGCCTTGCTGGCGCCATCGGCACCAGCGCCATGCCGGTGCGCGAGGCGGTGTCGCGC
CTGGTGGCTGAGAATGCGCTGGAAGTCCTCCCCAACCGGGCGGTGCGGGTGCCGCTGATGCCGCGCGCCCGCTTCCAGGA
ACTGCGCCTCATCCGCTGCAGCCTCGAAGGCCTCGCCACCGAGATCGCCGTGCGCGAGGCGAGCGACGCCGAGATCGCGG
AAGTGGAGCGCTTCGAGCGCCTGTTCGCCGCCGAGCGGGACAAGCCGCAGCCGGACGGCGCTGAAGCCATGCGCCACAAC
AAGGACCTGCATTTCGCGCTCTACCGCGCCGCCCATCTGCCCGCCTTGTTCCAGATGATCGAGGGGCTGTGGCTGCAGAT
CGGCCCGGTGCTGAACCTCGATTTCCGCGCCGGTCCCGAGCGCGTGCGCCAGGGCGAGGCGCACATCCGCCATGCAGCCA
TGGTGGCCGCCCTCAAGGCCCGTGACCCGGCGGCTGCCCGAACCGCCCTCGTCGCCGATATCTGGAGCGCGGGGGACTTC
ATCCTGTCCCGCGATGTCCTGCCGGACTGA

Upstream 100 bases:

>100_bases
GTGCTGCGGAGGCGGGATTTTCCCTGTCCAACCCGCACCGGGTGATTGCCGATAGGCTCGGCTATCTGTTATCTGTGATC
ACACATAGCAGACGGGAAAC

Downstream 100 bases:

>100_bases
CGCGGCTTTCACACCCAGAACAACAAGCACTTCAACGGAGAAACGTCATGGACCTCGGCATCACCGGAATCCGCGTGATC
ATCACCGCGGGCGCCGGGGG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MEHKSASADEREDGAAGEASGGLDARVGRIVRQTLADQVYGDLKEILLSGRAAPGERFTLRGLAGAIGTSAMPVREAVSR
LVAENALEVLPNRAVRVPLMPRARFQELRLIRCSLEGLATEIAVREASDAEIAEVERFERLFAAERDKPQPDGAEAMRHN
KDLHFALYRAAHLPALFQMIEGLWLQIGPVLNLDFRAGPERVRQGEAHIRHAAMVAALKARDPAAARTALVADIWSAGDF
ILSRDVLPD

Sequences:

>Translated_249_residues
MEHKSASADEREDGAAGEASGGLDARVGRIVRQTLADQVYGDLKEILLSGRAAPGERFTLRGLAGAIGTSAMPVREAVSR
LVAENALEVLPNRAVRVPLMPRARFQELRLIRCSLEGLATEIAVREASDAEIAEVERFERLFAAERDKPQPDGAEAMRHN
KDLHFALYRAAHLPALFQMIEGLWLQIGPVLNLDFRAGPERVRQGEAHIRHAAMVAALKARDPAAARTALVADIWSAGDF
ILSRDVLPD
>Mature_249_residues
MEHKSASADEREDGAAGEASGGLDARVGRIVRQTLADQVYGDLKEILLSGRAAPGERFTLRGLAGAIGTSAMPVREAVSR
LVAENALEVLPNRAVRVPLMPRARFQELRLIRCSLEGLATEIAVREASDAEIAEVERFERLFAAERDKPQPDGAEAMRHN
KDLHFALYRAAHLPALFQMIEGLWLQIGPVLNLDFRAGPERVRQGEAHIRHAAMVAALKARDPAAARTALVADIWSAGDF
ILSRDVLPD

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=221, Percent_Identity=33.9366515837104, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27166; Mature: 27166

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKSASADEREDGAAGEASGGLDARVGRIVRQTLADQVYGDLKEILLSGRAAPGERFTL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
RGLAGAIGTSAMPVREAVSRLVAENALEVLPNRAVRVPLMPRARFQELRLIRCSLEGLAT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
EIAVREASDAEIAEVERFERLFAAERDKPQPDGAEAMRHNKDLHFALYRAAHLPALFQMI
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EGLWLQIGPVLNLDFRAGPERVRQGEAHIRHAAMVAALKARDPAAARTALVADIWSAGDF
HHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCH
ILSRDVLPD
HCCCCCCCC
>Mature Secondary Structure
MEHKSASADEREDGAAGEASGGLDARVGRIVRQTLADQVYGDLKEILLSGRAAPGERFTL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
RGLAGAIGTSAMPVREAVSRLVAENALEVLPNRAVRVPLMPRARFQELRLIRCSLEGLAT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
EIAVREASDAEIAEVERFERLFAAERDKPQPDGAEAMRHNKDLHFALYRAAHLPALFQMI
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EGLWLQIGPVLNLDFRAGPERVRQGEAHIRHAAMVAALKARDPAAARTALVADIWSAGDF
HHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCH
ILSRDVLPD
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]