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The map label for this gene is pyrF [H]

Identifier: 154244314

GI number: 154244314

Start: 370828

End: 371532

Strand: Direct

Name: pyrF [H]

Synonym: Xaut_0357

Alternate gene names: 154244314

Gene position: 370828-371532 (Clockwise)

Preceding gene: 154244313

Following gene: 154244315

Centisome position: 6.98

GC content: 72.34

Gene sequence:

>705_bases
ATGAGCCTTGTCGATGCGAAAGTCCGCGACCGCATGATCGTGGCGCTCGACTATCCCAACGTGAGCGCGGCCGAGGCCCT
GGTCTGGCAGCTCGGCGACGTGGTGACCTTCTACAAGATCGGCCTGGAGCTCTCCATCGCGGGGGGGCTCGACCTTGCCG
CCGACCTCATCGGCGCCGGCAAGAAGGTGTTCCTCGATCTCAAGCTGCACGACATCGGCAATACGGTGGAGCGGGCGACC
GAGGCGGCGGCGGCGCGCGGGGTGCACATCCTCACCGTCCATGCCTATCCGCAGACCATGGCGGCGGCGGCGCGGGGGGC
CAAGGGCAGCCCGCTCGAAGTGCTGGGCGTGACCGTGCTCACCTCCTATGACGACGCGGACCTCGCCACCGCCGGCTATC
GCATGGGGGTGACCGACACGGTCCGCCTGCGCGCCGGGCAGGCTCTGGAGGCAGGGATCGCGGGGGTCATCTGCGCGCCC
ACGGATGCGGCCATGGTGCGCGCCATCCTGGGGCCGGATCGCCGGGTGGTGACGCCCGGGGTGCGGCCGAAGGGGGCGGA
CGTGGGTGACCAGAAGCGCATCGCCACCCCGGCTGAAGCCATCGCCGCGGGCGCCGACCGCATCGTGGTGGGCCGCCCGG
TGACGGCGGCCGCGGACCCGGTGGCGGCGGCCGAGGCCATCCTGGCGGAACTGGCGGGGGCCTGA

Upstream 100 bases:

>100_bases
AAGAGGGCGCGGCCCAGGCCGGCGTCCGTGACGACAGCACAGCGCCAGCCGGCGCGCGGGAACAGGGCGCAGCGCTGGCA
TGCGTCCCGGAGGAGGGCCC

Downstream 100 bases:

>100_bases
CGGCCGGCAACAGGGCAATGGAGGGGGCGATGGCGAAGGGCTACTGGTTCCTCAATCTCGATGTGGTCAACCCGGTGGAC
TACATCACCTACCGGGAAGC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSLVDAKVRDRMIVALDYPNVSAAEALVWQLGDVVTFYKIGLELSIAGGLDLAADLIGAGKKVFLDLKLHDIGNTVERAT
EAAAARGVHILTVHAYPQTMAAAARGAKGSPLEVLGVTVLTSYDDADLATAGYRMGVTDTVRLRAGQALEAGIAGVICAP
TDAAMVRAILGPDRRVVTPGVRPKGADVGDQKRIATPAEAIAAGADRIVVGRPVTAAADPVAAAEAILAELAGA

Sequences:

>Translated_234_residues
MSLVDAKVRDRMIVALDYPNVSAAEALVWQLGDVVTFYKIGLELSIAGGLDLAADLIGAGKKVFLDLKLHDIGNTVERAT
EAAAARGVHILTVHAYPQTMAAAARGAKGSPLEVLGVTVLTSYDDADLATAGYRMGVTDTVRLRAGQALEAGIAGVICAP
TDAAMVRAILGPDRRVVTPGVRPKGADVGDQKRIATPAEAIAAGADRIVVGRPVTAAADPVAAAEAILAELAGA
>Mature_233_residues
SLVDAKVRDRMIVALDYPNVSAAEALVWQLGDVVTFYKIGLELSIAGGLDLAADLIGAGKKVFLDLKLHDIGNTVERATE
AAAARGVHILTVHAYPQTMAAAARGAKGSPLEVLGVTVLTSYDDADLATAGYRMGVTDTVRLRAGQALEAGIAGVICAPT
DAAMVRAILGPDRRVVTPGVRPKGADVGDQKRIATPAEAIAAGADRIVVGRPVTAAADPVAAAEAILAELAGA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=226, Percent_Identity=39.3805309734513, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 23912; Mature: 23781

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVDAKVRDRMIVALDYPNVSAAEALVWQLGDVVTFYKIGLELSIAGGLDLAADLIGAG
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECEEEEECCCCHHHHHHHCCC
KKVFLDLKLHDIGNTVERATEAAAARGVHILTVHAYPQTMAAAARGAKGSPLEVLGVTVL
CEEEEEEEEHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEE
TSYDDADLATAGYRMGVTDTVRLRAGQALEAGIAGVICAPTDAAMVRAILGPDRRVVTPG
ECCCCCCHHHCCEECCCCEEEEECCCCHHHCCCCEEEECCCHHHHHHHHHCCCCEEECCC
VRPKGADVGDQKRIATPAEAIAAGADRIVVGRPVTAAADPVAAAEAILAELAGA
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLVDAKVRDRMIVALDYPNVSAAEALVWQLGDVVTFYKIGLELSIAGGLDLAADLIGAG
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECEEEEECCCCHHHHHHHCCC
KKVFLDLKLHDIGNTVERATEAAAARGVHILTVHAYPQTMAAAARGAKGSPLEVLGVTVL
CEEEEEEEEHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEE
TSYDDADLATAGYRMGVTDTVRLRAGQALEAGIAGVICAPTDAAMVRAILGPDRRVVTPG
ECCCCCCHHHCCEECCCCEEEEECCCCHHHCCCCEEEECCCHHHHHHHHHCCCCEEECCC
VRPKGADVGDQKRIATPAEAIAAGADRIVVGRPVTAAADPVAAAEAILAELAGA
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA