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The map label for this gene is nusA

Identifier: 154244252

GI number: 154244252

Start: 302397

End: 303992

Strand: Reverse

Name: nusA

Synonym: Xaut_0295

Alternate gene names: 154244252

Gene position: 303992-302397 (Counterclockwise)

Preceding gene: 154244253

Following gene: 154244251

Centisome position: 5.73

GC content: 68.61

Gene sequence:

>1596_bases
ATGGTTGCCGTCAGCGCAAACCGGCTGGAACTGCTGCAGATCGCCGATGCGGTCGCGCGGGAAAAGTCCATCGACCGCAG
CATCGTGATTGCCGCCATGGAGGACGCCATCGCCAAGGCCGCGCGCTCGCGCTACGGCCAGGAGACGGACATCCACGCCG
ACATCAACGCCAAGACCGGCGAGCTGCGTCTGGCCCGCCACCTGCTGGTGGTGGACGAGGTGGAGAACACGGCCACCGAG
ATCACCCTGGACGGCGCCCGGCGCCACAACCCGGCGGCGCAGGTGGGCGACACCATCGCCGACACCCTGCCCCCGTTCGA
TTTCGGCCGCATCGCGGCCCAGTCGGCCAAGCAGGTCATCGTGCAGAAGGTGCGCGAGGCCGAGCGCGACCGGCAGTATG
ACGAATACAAGGACCGCATCGGCGACGTGGTGAACGGCCTCGTCAAGCGCGTCGAATACGGCAACGTGGTGGTGGACCTC
GGCCGTGGCGAAGGCATCCTGCGCCGCGACGAGCTTTTGCCGCGCGAGACGGTGAAGACCGGCGACCGCATCCGCGCTTA
CGTCTACGACGTGCGCCGCGAGCCGCGCGGGCCACAGATCTTCCTGTCGCGCACCCATCCCCAGTTCATGGCCAAGCTGT
TCGCGCAGGAAGTGCCCGAGATCTACGACGGCATCGTCGAGATCAAGGCGGTGGCGCGCGATCCCGGCTCCCGCGCCAAG
ATCGCGGTCATCTCCCGCGACCAGTCGGTGGACCCCGTCGGCGCCTGCGTCGGCATGCGCGGCTCGCGCGTGCAGGCGGT
GGTGAACGAGCTGCAGGGCGAGAAGATCGACATCATCCCCTGGAACCCGGACATCGCCACCTTCATCGTCAACGCCCTCG
CCCCCGCCGAGGTGGTGAAGGTGGTGCTCGACGAGGATCGCGAGCGCATCGAGGTGGTGGTGCCCGACGCCCAGCTGTCG
CTGGCCATCGGCCGCCGCGGCCAGAACGTGCGCCTCGCCACCCAGCTCACCGGCTGGGACATCGACATCATGACCGAGCA
GGAGGAAAGCGAGCGGCGCCAGAAGGAATTCGCCGAGCGCACCAAGATGTTCGCCGAGGCCCTCGATCTCGACGAGATGA
TGGGCCAGCTGCTGACCTCCGAGGGCTTCACCTCGGTGGAGGAGATTGCCTACGTCCAGCTCCAGGAGCTTGCCTCCATC
GAGGGCTTCGACGAGGACACCGCCGCCGAGCTGCAGGCCCGTGCCCTGAAGCACCTCGCCGAGGTGGAAGAGGCGCTCGA
CGCCCGCCGCATCGAGCTGGGCGTGGACGACGACCTGAAGAGCGTGCCCGGCGTCACCTCCAAGATGCTGGTCGCGTTCG
GCGAGAACGACATCAAGAGCGTCGAGGACCTGGCCGGCTGCGCCACCGATGACCTCATCGGCTGGACCGAGCGCAAGGAC
GGCGAGACCGTCCGCCACGCCGGCGCCCTCGATGGCTTCGAACTGTCCCGCGAGGACGCGGAGGCCCTGGTCATGCAGGC
CCGCGTCGCCGCCGGCTGGATCGAGGCAGACGAACCCGCTGCGGATGGCGATGACGCCGCCGCCGGCGAAGCCTGA

Upstream 100 bases:

>100_bases
GCAAGAAGGCTTCGCCCAAGAAATCCAACGCCAAGAAGAAGGCCGGCCTGGAAACGGCCGCGTCCTCGGCCAACGCCAGC
ACCGTGAAGGAGACGCACTG

Downstream 100 bases:

>100_bases
GACTGGGAGATGACACACGGTGCCCGCTATGGTGGACGAGGACGAGACGGACGAGGGACTGCGCAGCCTTGCGGCGGCGC
GGGCGCCCCTGTCGCGCCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MVAVSANRLELLQIADAVAREKSIDRSIVIAAMEDAIAKAARSRYGQETDIHADINAKTGELRLARHLLVVDEVENTATE
ITLDGARRHNPAAQVGDTIADTLPPFDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGDVVNGLVKRVEYGNVVVDL
GRGEGILRRDELLPRETVKTGDRIRAYVYDVRREPRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAK
IAVISRDQSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNPDIATFIVNALAPAEVVKVVLDEDRERIEVVVPDAQLS
LAIGRRGQNVRLATQLTGWDIDIMTEQEESERRQKEFAERTKMFAEALDLDEMMGQLLTSEGFTSVEEIAYVQLQELASI
EGFDEDTAAELQARALKHLAEVEEALDARRIELGVDDDLKSVPGVTSKMLVAFGENDIKSVEDLAGCATDDLIGWTERKD
GETVRHAGALDGFELSREDAEALVMQARVAAGWIEADEPAADGDDAAAGEA

Sequences:

>Translated_531_residues
MVAVSANRLELLQIADAVAREKSIDRSIVIAAMEDAIAKAARSRYGQETDIHADINAKTGELRLARHLLVVDEVENTATE
ITLDGARRHNPAAQVGDTIADTLPPFDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGDVVNGLVKRVEYGNVVVDL
GRGEGILRRDELLPRETVKTGDRIRAYVYDVRREPRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAK
IAVISRDQSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNPDIATFIVNALAPAEVVKVVLDEDRERIEVVVPDAQLS
LAIGRRGQNVRLATQLTGWDIDIMTEQEESERRQKEFAERTKMFAEALDLDEMMGQLLTSEGFTSVEEIAYVQLQELASI
EGFDEDTAAELQARALKHLAEVEEALDARRIELGVDDDLKSVPGVTSKMLVAFGENDIKSVEDLAGCATDDLIGWTERKD
GETVRHAGALDGFELSREDAEALVMQARVAAGWIEADEPAADGDDAAAGEA
>Mature_531_residues
MVAVSANRLELLQIADAVAREKSIDRSIVIAAMEDAIAKAARSRYGQETDIHADINAKTGELRLARHLLVVDEVENTATE
ITLDGARRHNPAAQVGDTIADTLPPFDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGDVVNGLVKRVEYGNVVVDL
GRGEGILRRDELLPRETVKTGDRIRAYVYDVRREPRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAK
IAVISRDQSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNPDIATFIVNALAPAEVVKVVLDEDRERIEVVVPDAQLS
LAIGRRGQNVRLATQLTGWDIDIMTEQEESERRQKEFAERTKMFAEALDLDEMMGQLLTSEGFTSVEEIAYVQLQELASI
EGFDEDTAAELQARALKHLAEVEEALDARRIELGVDDDLKSVPGVTSKMLVAFGENDIKSVEDLAGCATDDLIGWTERKD
GETVRHAGALDGFELSREDAEALVMQARVAAGWIEADEPAADGDDAAAGEA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=488, Percent_Identity=43.0327868852459, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 58442; Mature: 58442

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVSANRLELLQIADAVAREKSIDRSIVIAAMEDAIAKAARSRYGQETDIHADINAKTG
CEECCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
ELRLARHLLVVDEVENTATEITLDGARRHNPAAQVGDTIADTLPPFDFGRIAAQSAKQVI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VQKVREAERDRQYDEYKDRIGDVVNGLVKRVEYGNVVVDLGRGEGILRRDELLPRETVKT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEHHHCCCHHHHCC
GDRIRAYVYDVRREPRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAK
CHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IAVISRDQSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNPDIATFIVNALAPAEVVK
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHH
VVLDEDRERIEVVVPDAQLSLAIGRRGQNVRLATQLTGWDIDIMTEQEESERRQKEFAER
HHHHCCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHH
TKMFAEALDLDEMMGQLLTSEGFTSVEEIAYVQLQELASIEGFDEDTAAELQARALKHLA
HHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVEEALDARRIELGVDDDLKSVPGVTSKMLVAFGENDIKSVEDLAGCATDDLIGWTERKD
HHHHHHHHHEEEECCCCCHHHCCCCHHHHHEEECCCHHHHHHHHHCCCCHHHCCCCCCCC
GETVRHAGALDGFELSREDAEALVMQARVAAGWIEADEPAADGDDAAAGEA
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAVSANRLELLQIADAVAREKSIDRSIVIAAMEDAIAKAARSRYGQETDIHADINAKTG
CEECCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
ELRLARHLLVVDEVENTATEITLDGARRHNPAAQVGDTIADTLPPFDFGRIAAQSAKQVI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VQKVREAERDRQYDEYKDRIGDVVNGLVKRVEYGNVVVDLGRGEGILRRDELLPRETVKT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEHHHCCCHHHHCC
GDRIRAYVYDVRREPRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAK
CHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IAVISRDQSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNPDIATFIVNALAPAEVVK
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHH
VVLDEDRERIEVVVPDAQLSLAIGRRGQNVRLATQLTGWDIDIMTEQEESERRQKEFAER
HHHHCCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHH
TKMFAEALDLDEMMGQLLTSEGFTSVEEIAYVQLQELASIEGFDEDTAAELQARALKHLA
HHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EVEEALDARRIELGVDDDLKSVPGVTSKMLVAFGENDIKSVEDLAGCATDDLIGWTERKD
HHHHHHHHHEEEECCCCCHHHCCCCHHHHHEEECCCHHHHHHHHHCCCCHHHCCCCCCCC
GETVRHAGALDGFELSREDAEALVMQARVAAGWIEADEPAADGDDAAAGEA
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]