Click here to switch to the map view.

The map label for this gene is nifV [H]

Identifier: 154244108

GI number: 154244108

Start: 144010

End: 145257

Strand: Direct

Name: nifV [H]

Synonym: Xaut_0150

Alternate gene names: 154244108

Gene position: 144010-145257 (Clockwise)

Preceding gene: 154244107

Following gene: 154244109

Centisome position: 2.71

GC content: 70.03

Gene sequence:

>1248_bases
ATGCTCGCGAAGTCACCATCGGCCATTCTGGCGCGCTCCAGCCGGGCATCCGTGGATGCCAGCGGCGCCGCCACCGGGAC
GGTGCCGGCCCGGACGGCTTTCCTGAACGACACCACCCTCCGGGATGGTGAGCAGGCGCCGGGCGTCGCTTTCACCCGCC
GCGAGAAGATCGAGATCGCCGAGGCGCTGGCTGCGGCCGGCGTGCCCGAGATCGAGGCCGGCACTCCGGCCATGGGCGAC
GAGGAAATCGAGACCCTCCGCTCCATCGTCTCCCTCAAGCTGCCCCTGCGCGTCGCCGCCTGGTGCCGCATGAGCGAGGA
AGATCTCTTCGCCGCGGTCTCCGCCGGCGTCACCTATGTGAACCTGTCTATCCCCACCTCCGACCGCCAGCTGAAGGGCA
AGCTCAACCGCGACCGCGACTGGGCGCTGGGGGCCCTGCGGCATGTGGTGACGCTGGCGACGAAGCTCGGCTTCACCGCT
GCCGTGGGCTGCGAGGATGCCTCCCGCGCCGATCCCGACTTCCTGTGCAAGGTGGCCGAGGTGGCGAAGGAGGCGGGCGC
CTTCCGCCTGCGCCTCGCCGATACGCTGGGCGTGCTTGATCCCTTCTCCGCCTATGCGCTGGTGCGGCGCGTGGCCAATG
CGACGGACCTCGAGATCGAGTTCCACGCCCACGACGACCTTGGCCTCGCCACGGCCAACACGCTTGCCGCCGTCACCGGT
GGGGCCCGACATGCCAGCGTGACGGTGGCCGGCCTTGGCGAACGGGCGGGCAACGCGGCGCTGGAGGAAGTGGCCATCGC
CCTGCGCCAGACTGCGCGCTCGGAAACCGGCATCGACCCGCATGCCCTGCGGCCCTTGGCCCAGAAGGTGATGGAGGCGG
CCGGCCGCCCCATGCCCCGCACCAAGGCCATCGTGGGAGAAGACATCTTCACCCACGAATCCGGCATCCACGTCTCCGGC
CTGCTGAAGGACCGCGGCACCTACGAGGCGCTCAGCCCCGAGATGCTGGGCCGCTCGCACCGGGTGGTGCTGGGCAAGCA
TTCTGGCCTCGCCGCCATCCTCAAGGCCCTGTCGGACCAGGGGCTTGCGGTGGACGAGGCACGCGGCCGCGCCATCCTTG
CCCGGGTGCGCGACTTCGCCATCCGTTCGAAGAACTCGGTCTCCCCGGACGTGCTCAGGCGCTTCTACGAAGACAGCTTC
ACCGAAGAGGCGCTCAAGCGCCGCCACGCCGCAGTGATGGGAGCCTGA

Upstream 100 bases:

>100_bases
GGCAGGCGGCGGCTTGCGGAACTGACGAGGGTCCGGGCCCAGCCCGGTGGAGTTCATTCATCCCTCGCCGGCGCGGCGGG
GGGTAGATCGGAAGGGACCC

Downstream 100 bases:

>100_bases
CCCATGATCATGGGAAGCCCGAAATGGCAGGGGACCGACGTTCCGCTCGCCGGCGCGGCGGAGGCTGAGAAGGCGTCCGC
CCTCTCGGTGTGGCGCCTCA

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLAKSPSAILARSSRASVDASGAATGTVPARTAFLNDTTLRDGEQAPGVAFTRREKIEIAEALAAAGVPEIEAGTPAMGD
EEIETLRSIVSLKLPLRVAAWCRMSEEDLFAAVSAGVTYVNLSIPTSDRQLKGKLNRDRDWALGALRHVVTLATKLGFTA
AVGCEDASRADPDFLCKVAEVAKEAGAFRLRLADTLGVLDPFSAYALVRRVANATDLEIEFHAHDDLGLATANTLAAVTG
GARHASVTVAGLGERAGNAALEEVAIALRQTARSETGIDPHALRPLAQKVMEAAGRPMPRTKAIVGEDIFTHESGIHVSG
LLKDRGTYEALSPEMLGRSHRVVLGKHSGLAAILKALSDQGLAVDEARGRAILARVRDFAIRSKNSVSPDVLRRFYEDSF
TEEALKRRHAAVMGA

Sequences:

>Translated_415_residues
MLAKSPSAILARSSRASVDASGAATGTVPARTAFLNDTTLRDGEQAPGVAFTRREKIEIAEALAAAGVPEIEAGTPAMGD
EEIETLRSIVSLKLPLRVAAWCRMSEEDLFAAVSAGVTYVNLSIPTSDRQLKGKLNRDRDWALGALRHVVTLATKLGFTA
AVGCEDASRADPDFLCKVAEVAKEAGAFRLRLADTLGVLDPFSAYALVRRVANATDLEIEFHAHDDLGLATANTLAAVTG
GARHASVTVAGLGERAGNAALEEVAIALRQTARSETGIDPHALRPLAQKVMEAAGRPMPRTKAIVGEDIFTHESGIHVSG
LLKDRGTYEALSPEMLGRSHRVVLGKHSGLAAILKALSDQGLAVDEARGRAILARVRDFAIRSKNSVSPDVLRRFYEDSF
TEEALKRRHAAVMGA
>Mature_415_residues
MLAKSPSAILARSSRASVDASGAATGTVPARTAFLNDTTLRDGEQAPGVAFTRREKIEIAEALAAAGVPEIEAGTPAMGD
EEIETLRSIVSLKLPLRVAAWCRMSEEDLFAAVSAGVTYVNLSIPTSDRQLKGKLNRDRDWALGALRHVVTLATKLGFTA
AVGCEDASRADPDFLCKVAEVAKEAGAFRLRLADTLGVLDPFSAYALVRRVANATDLEIEFHAHDDLGLATANTLAAVTG
GARHASVTVAGLGERAGNAALEEVAIALRQTARSETGIDPHALRPLAQKVMEAAGRPMPRTKAIVGEDIFTHESGIHVSG
LLKDRGTYEALSPEMLGRSHRVVLGKHSGLAAILKALSDQGLAVDEARGRAILARVRDFAIRSKNSVSPDVLRRFYEDSF
TEEALKRRHAAVMGA

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=349, Percent_Identity=35.5300859598854, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320019, Length=379, Percent_Identity=29.5514511873351, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320071, Length=348, Percent_Identity=29.3103448275862, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24582381, Length=280, Percent_Identity=26.7857142857143, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 44040; Mature: 44040

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKSPSAILARSSRASVDASGAATGTVPARTAFLNDTTLRDGEQAPGVAFTRREKIEIA
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCEEHHHHHHHHH
EALAAAGVPEIEAGTPAMGDEEIETLRSIVSLKLPLRVAAWCRMSEEDLFAAVSAGVTYV
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCEEEE
NLSIPTSDRQLKGKLNRDRDWALGALRHVVTLATKLGFTAAVGCEDASRADPDFLCKVAE
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHH
VAKEAGAFRLRLADTLGVLDPFSAYALVRRVANATDLEIEFHAHDDLGLATANTLAAVTG
HHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCC
GARHASVTVAGLGERAGNAALEEVAIALRQTARSETGIDPHALRPLAQKVMEAAGRPMPR
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
TKAIVGEDIFTHESGIHVSGLLKDRGTYEALSPEMLGRSHRVVLGKHSGLAAILKALSDQ
HHHHHCCHHHCCCCCCEEEEEECCCCCHHHCCHHHCCCCCEEEEECCCCHHHHHHHHCCC
GLAVDEARGRAILARVRDFAIRSKNSVSPDVLRRFYEDSFTEEALKRRHAAVMGA
CCEEEHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAKSPSAILARSSRASVDASGAATGTVPARTAFLNDTTLRDGEQAPGVAFTRREKIEIA
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCEEHHHHHHHHH
EALAAAGVPEIEAGTPAMGDEEIETLRSIVSLKLPLRVAAWCRMSEEDLFAAVSAGVTYV
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCEEEE
NLSIPTSDRQLKGKLNRDRDWALGALRHVVTLATKLGFTAAVGCEDASRADPDFLCKVAE
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHH
VAKEAGAFRLRLADTLGVLDPFSAYALVRRVANATDLEIEFHAHDDLGLATANTLAAVTG
HHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCC
GARHASVTVAGLGERAGNAALEEVAIALRQTARSETGIDPHALRPLAQKVMEAAGRPMPR
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
TKAIVGEDIFTHESGIHVSGLLKDRGTYEALSPEMLGRSHRVVLGKHSGLAAILKALSDQ
HHHHHCCHHHCCCCCCEEEEEECCCCCHHHCCHHHCCCCCEEEEECCCCHHHHHHHHCCC
GLAVDEARGRAILARVRDFAIRSKNSVSPDVLRRFYEDSFTEEALKRRHAAVMGA
CCEEEHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]