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The map label for this gene is nifS [H]

Identifier: 154244066

GI number: 154244066

Start: 113138

End: 114340

Strand: Direct

Name: nifS [H]

Synonym: Xaut_0108

Alternate gene names: 154244066

Gene position: 113138-114340 (Clockwise)

Preceding gene: 154244065

Following gene: 154244067

Centisome position: 2.13

GC content: 66.75

Gene sequence:

>1203_bases
GTGCGGCCCGTCTATCTCGACAACAACGCGACGACACGGACCGACCCCGCTGTGGTCGAGGCCATGCTGCCGTTCTTCAG
CGAGCATTTCGGCAACGCCTCCTCCACCCACGCCTTCGGTGAGCACGTGGGCGAGGCGCTGAAGAAGGCGCGCAAGCAGC
TCCAGGCGCTGCTTGGCGCCGCCTTTGACCACGAGATCATCTACACCTCCGGCGGAACGGAAGCCGACAATGCGGCCATT
CTTTCCGCGCTGGAGACGCAGGAAGGCCGGGACGAGATCGTCACCACCACGGTGGAGCATCCGGCGATCCTGACCCTGGT
CGCGCATCTGGAAAAGACGCGCGGCATCAAAGTGCATCTCATCGGTGTGGACCGCCATGGGCGGCTCGATCTCGATGCCT
ACGCCAAGGCATTGTCGCCGAAGACCGCTCTCGTCTCGGTGATGTGGGCCAACAACGAGACAGGAACCCTCTTCCCCGTG
GCTGAGCTGGCAGAGCAGGCGAAGGCCGTCGGCGCCCTGTTCCATACCGACGCGGTGCAGGCGGTGGGTAAGGTGGCCAT
CGACCTGAAATCCACAGCCATCGACATGCTGTCCCTGTCCGGCCACAAGCTGCACGGGCCCAAGGGCATCGGCGCGCTCT
ATGTGAAGAAGGGGGTGAAGTTCCGCCCCCTCATCCGCGGCGGCCATCAGGAGCGCGGGCGGCGTGGCGGAACCGAGAAT
GTGCCCGGTATCATCGGACTCGGCGCTGCTGCGGACCTCGCGCTCCAGCACATGGAGGAGGAGCGCACCCGCGTGAAGGC
GCTGCGCGACCGGCTCGAGCAGGGCATCCTCCAGCGGGTGAGCAACACCCTGCTCAATGGCGATGGCGACAACCGCCTCC
CCAACACCGCAAATATCGCGTTCGAATATCTCGACGGCGAGGCGGTGCTGCACAATCTCAACAAGGCCGGAGTTGCCGCC
TCCACCGGCTCGGCCTGCACCTCAGGCTCCACCGAGCCGTCCCATGTGCTGCGGGCCATGAACCTGCCGGCCACTGCGCT
CCATGGCGCCCTGCGCCTGTCGCTTTCCCGCGAGAACACGGGGGAGGACGTGGACCGGGTGCTGGAAGCGCTGCCGGAGA
TCGTCACCCGCCTGCGCGCCATGTCGCCGGCCTGGCTGGCGCGGGAGGGTGCGGACGGCGCGGCGGTGGCCAGTCATGTC
TAG

Upstream 100 bases:

>100_bases
GCCGGCTCCGACCGCCGCGCCGGTGGCTCCGGCCCCCGCCGTCCCCTCGCCGACGCGCTGAGGGACGAGCCAAGGTCTAG
ACGATCGAAGGGGTGATGCC

Downstream 100 bases:

>100_bases
CTTTCATCCGGGGCGCGAGTGCGGCGGACGCACGCCCACCGGCGTCGCCTTCGCGATGGCAAGCGCGTGCCCGATGCCGG
CCGACGTGGCGGCCGCCGGG

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRPVYLDNNATTRTDPAVVEAMLPFFSEHFGNASSTHAFGEHVGEALKKARKQLQALLGAAFDHEIIYTSGGTEADNAAI
LSALETQEGRDEIVTTTVEHPAILTLVAHLEKTRGIKVHLIGVDRHGRLDLDAYAKALSPKTALVSVMWANNETGTLFPV
AELAEQAKAVGALFHTDAVQAVGKVAIDLKSTAIDMLSLSGHKLHGPKGIGALYVKKGVKFRPLIRGGHQERGRRGGTEN
VPGIIGLGAAADLALQHMEEERTRVKALRDRLEQGILQRVSNTLLNGDGDNRLPNTANIAFEYLDGEAVLHNLNKAGVAA
STGSACTSGSTEPSHVLRAMNLPATALHGALRLSLSRENTGEDVDRVLEALPEIVTRLRAMSPAWLAREGADGAAVASHV

Sequences:

>Translated_400_residues
MRPVYLDNNATTRTDPAVVEAMLPFFSEHFGNASSTHAFGEHVGEALKKARKQLQALLGAAFDHEIIYTSGGTEADNAAI
LSALETQEGRDEIVTTTVEHPAILTLVAHLEKTRGIKVHLIGVDRHGRLDLDAYAKALSPKTALVSVMWANNETGTLFPV
AELAEQAKAVGALFHTDAVQAVGKVAIDLKSTAIDMLSLSGHKLHGPKGIGALYVKKGVKFRPLIRGGHQERGRRGGTEN
VPGIIGLGAAADLALQHMEEERTRVKALRDRLEQGILQRVSNTLLNGDGDNRLPNTANIAFEYLDGEAVLHNLNKAGVAA
STGSACTSGSTEPSHVLRAMNLPATALHGALRLSLSRENTGEDVDRVLEALPEIVTRLRAMSPAWLAREGADGAAVASHV
>Mature_400_residues
MRPVYLDNNATTRTDPAVVEAMLPFFSEHFGNASSTHAFGEHVGEALKKARKQLQALLGAAFDHEIIYTSGGTEADNAAI
LSALETQEGRDEIVTTTVEHPAILTLVAHLEKTRGIKVHLIGVDRHGRLDLDAYAKALSPKTALVSVMWANNETGTLFPV
AELAEQAKAVGALFHTDAVQAVGKVAIDLKSTAIDMLSLSGHKLHGPKGIGALYVKKGVKFRPLIRGGHQERGRRGGTEN
VPGIIGLGAAADLALQHMEEERTRVKALRDRLEQGILQRVSNTLLNGDGDNRLPNTANIAFEYLDGEAVLHNLNKAGVAA
STGSACTSGSTEPSHVLRAMNLPATALHGALRLSLSRENTGEDVDRVLEALPEIVTRLRAMSPAWLAREGADGAAVASHV

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=397, Percent_Identity=41.8136020151133, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=35.3365384615385, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI48994898, Length=397, Percent_Identity=41.8136020151133, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789175, Length=388, Percent_Identity=28.0927835051546, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787970, Length=395, Percent_Identity=28.3544303797468, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=39.9002493765586, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=31.4814814814815, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=396, Percent_Identity=40.4040404040404, Blast_Score=289, Evalue=5e-79,
Organism=Drosophila melanogaster, GI20129463, Length=396, Percent_Identity=41.6666666666667, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42604; Mature: 42604

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVYLDNNATTRTDPAVVEAMLPFFSEHFGNASSTHAFGEHVGEALKKARKQLQALLGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFDHEIIYTSGGTEADNAAILSALETQEGRDEIVTTTVEHPAILTLVAHLEKTRGIKVHL
HCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHHHCCCEEEE
IGVDRHGRLDLDAYAKALSPKTALVSVMWANNETGTLFPVAELAEQAKAVGALFHTDAVQ
EEECCCCCCCHHHHHHHCCCCHHEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
AVGKVAIDLKSTAIDMLSLSGHKLHGPKGIGALYVKKGVKFRPLIRGGHQERGRRGGTEN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCHHHHCCCCCCC
VPGIIGLGAAADLALQHMEEERTRVKALRDRLEQGILQRVSNTLLNGDGDNRLPNTANIA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEE
FEYLDGEAVLHNLNKAGVAASTGSACTSGSTEPSHVLRAMNLPATALHGALRLSLSRENT
EEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCCCC
GEDVDRVLEALPEIVTRLRAMSPAWLAREGADGAAVASHV
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MRPVYLDNNATTRTDPAVVEAMLPFFSEHFGNASSTHAFGEHVGEALKKARKQLQALLGA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFDHEIIYTSGGTEADNAAILSALETQEGRDEIVTTTVEHPAILTLVAHLEKTRGIKVHL
HCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHHHCCCEEEE
IGVDRHGRLDLDAYAKALSPKTALVSVMWANNETGTLFPVAELAEQAKAVGALFHTDAVQ
EEECCCCCCCHHHHHHHCCCCHHEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
AVGKVAIDLKSTAIDMLSLSGHKLHGPKGIGALYVKKGVKFRPLIRGGHQERGRRGGTEN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCHHHHCCCCCCC
VPGIIGLGAAADLALQHMEEERTRVKALRDRLEQGILQRVSNTLLNGDGDNRLPNTANIA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEE
FEYLDGEAVLHNLNKAGVAASTGSACTSGSTEPSHVLRAMNLPATALHGALRLSLSRENT
EEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCCCC
GEDVDRVLEALPEIVTRLRAMSPAWLAREGADGAAVASHV
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]