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The map label for this gene is sufS [H]

Identifier: 154244045

GI number: 154244045

Start: 94482

End: 95744

Strand: Direct

Name: sufS [H]

Synonym: Xaut_0087

Alternate gene names: 154244045

Gene position: 94482-95744 (Clockwise)

Preceding gene: 154244044

Following gene: 154244046

Centisome position: 1.78

GC content: 65.0

Gene sequence:

>1263_bases
ATGATGCTTCCTCTCCCCGCTCCGCACCCTGCCGTTTCCAACGGCAGCTATGACGTCCTGCGCGTACGCGAGGACTTTCC
CATCCTCGACCTGAAGGTCAACGGCAAGCCGCTGGTCTATCTGGATAATGGCGCCTCGGCCCAAAAGCCGCGGCAGGTGC
TGGACCGCATCCAGCAGGTTTACACCTCCGAATATGCCAACGTGCATCGCGGCCTGCACTACCTCGCCAATGCGGCCACC
GAGGCCTATGAGGGCGGGCGCGCCCGCGTCCAGCGCTTCCTCAATGCGGCGCGGCCGGAAGAGATCGTCTTCACCCGCAG
TGCGACGGAGGCGATCAACCTCGTCGCTGCCACCTTCGGGAAAGCCCGCATCAAGCCCGGCGATGAGATCGTGCTCTCCA
TCATGGAGCATCACGCCAATATCGTGCCCTGGCACTTCCTGCGGGAAAGCCTTGGTGCGGTGATCAAGTGGGCGCCGGTG
GACGAGGAAGGCAACTTCCTCATGGACGAGTTCGAGGCCCTGCTCACCGAGCGGACCAAGATGGTGGCCATCACCCAGAT
GTCCAACGTGCTTGGCACCACCGTTCCGGTGAAGGAGGTGGTGAAGATCGCCCACGCCCGCGGCATTCCGGTGCTGGTGG
ACGGATCCCAGGGCGCGGTCCATCTCGACGTGGACGTGCAGGATCTCGACTGCGACTTCTACATCATCACCGGCCACAAG
CTGTATGGGCCCACCGGCATCGGTGCGCTCTACGGCAAGTATGAGCACCTGGAGGCCATGCCGCCCTTCAACGGCGGCGG
CGAGATGATCCGCGAAGTGCGGCAGGACACCGTCACCTATGGCGATCCGCCCCACCGGTTCGAGGCGGGCACGCCGGCCA
TCGTGCAGGCGGCGGGCCTCGGTGCCGCGCTGGACTATATCGAATCCATCGGCAAGGCCCGCATCCGCGCCCATGAAGCG
GAACTCACCCGCTATGCGCAGGAGCGGCTCGGGGAGCTGAACTCGGTGCGCATCATCGGCACGGCCAAGGACAAGGGCGC
CATCATCTCGTTCGAGATCAAGGGCGCGCACGCCCACGATTTCGCGACGTTGATCGACAATGACGGCATCGCCGTGCGCG
CCGGCACCCATTGCGCCATGCCGCTGCTGGAGCGCTTCGGCGTTTCCGCCACCTGCCGCGCCTCTTTCGGCCTCTACAAC
ACACGCGAGGAAGTGGACCGCTTCGTCGCCGCCCTCTCGAAGGCGCAGGAGCTGATCGGCTGA

Upstream 100 bases:

>100_bases
CCAGGCCTTTGTGGGCGAGGTGCTCGACGCCATCGGCAACGAGGGCGTGCGCGCGGCGCTGACGGATGCGACCCAGGCCT
GGCTCCTGGGTCGGGAGTGA

Downstream 100 bases:

>100_bases
AGCTTCAGCGCACAACGTCTTGTCCCGGACCGGGCGGTCTTTTCCGCCCGGTCACGCGCTTCCCACCTATGATTTTACGC
CTCCCATTTTCAGATCGGGT

Product: SufS subfamily cysteine desulfurase

Products: NA

Alternate protein names: Selenocysteine beta-lyase; SCL; Selenocysteine lyase; Selenocysteine reductase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MMLPLPAPHPAVSNGSYDVLRVREDFPILDLKVNGKPLVYLDNGASAQKPRQVLDRIQQVYTSEYANVHRGLHYLANAAT
EAYEGGRARVQRFLNAARPEEIVFTRSATEAINLVAATFGKARIKPGDEIVLSIMEHHANIVPWHFLRESLGAVIKWAPV
DEEGNFLMDEFEALLTERTKMVAITQMSNVLGTTVPVKEVVKIAHARGIPVLVDGSQGAVHLDVDVQDLDCDFYIITGHK
LYGPTGIGALYGKYEHLEAMPPFNGGGEMIREVRQDTVTYGDPPHRFEAGTPAIVQAAGLGAALDYIESIGKARIRAHEA
ELTRYAQERLGELNSVRIIGTAKDKGAIISFEIKGAHAHDFATLIDNDGIAVRAGTHCAMPLLERFGVSATCRASFGLYN
TREEVDRFVAALSKAQELIG

Sequences:

>Translated_420_residues
MMLPLPAPHPAVSNGSYDVLRVREDFPILDLKVNGKPLVYLDNGASAQKPRQVLDRIQQVYTSEYANVHRGLHYLANAAT
EAYEGGRARVQRFLNAARPEEIVFTRSATEAINLVAATFGKARIKPGDEIVLSIMEHHANIVPWHFLRESLGAVIKWAPV
DEEGNFLMDEFEALLTERTKMVAITQMSNVLGTTVPVKEVVKIAHARGIPVLVDGSQGAVHLDVDVQDLDCDFYIITGHK
LYGPTGIGALYGKYEHLEAMPPFNGGGEMIREVRQDTVTYGDPPHRFEAGTPAIVQAAGLGAALDYIESIGKARIRAHEA
ELTRYAQERLGELNSVRIIGTAKDKGAIISFEIKGAHAHDFATLIDNDGIAVRAGTHCAMPLLERFGVSATCRASFGLYN
TREEVDRFVAALSKAQELIG
>Mature_420_residues
MMLPLPAPHPAVSNGSYDVLRVREDFPILDLKVNGKPLVYLDNGASAQKPRQVLDRIQQVYTSEYANVHRGLHYLANAAT
EAYEGGRARVQRFLNAARPEEIVFTRSATEAINLVAATFGKARIKPGDEIVLSIMEHHANIVPWHFLRESLGAVIKWAPV
DEEGNFLMDEFEALLTERTKMVAITQMSNVLGTTVPVKEVVKIAHARGIPVLVDGSQGAVHLDVDVQDLDCDFYIITGHK
LYGPTGIGALYGKYEHLEAMPPFNGGGEMIREVRQDTVTYGDPPHRFEAGTPAIVQAAGLGAALDYIESIGKARIRAHEA
ELTRYAQERLGELNSVRIIGTAKDKGAIISFEIKGAHAHDFATLIDNDGIAVRAGTHCAMPLLERFGVSATCRASFGLYN
TREEVDRFVAALSKAQELIG

Specific function: Cysteine desulfurases mobilize the sulfur from L- cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=391, Percent_Identity=27.1099744245524, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI156713448, Length=435, Percent_Identity=26.4367816091954, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787970, Length=405, Percent_Identity=47.6543209876543, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789175, Length=403, Percent_Identity=41.439205955335, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI48994898, Length=395, Percent_Identity=26.5822784810127, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=243, Percent_Identity=28.8065843621399, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193211090, Length=416, Percent_Identity=23.3173076923077, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=388, Percent_Identity=26.2886597938144, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129463, Length=297, Percent_Identity=29.6296296296296, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7; =4.4.1.16 [H]

Molecular weight: Translated: 45960; Mature: 45960

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLPLPAPHPAVSNGSYDVLRVREDFPILDLKVNGKPLVYLDNGASAQKPRQVLDRIQQV
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHH
YTSEYANVHRGLHYLANAATEAYEGGRARVQRFLNAARPEEIVFTRSATEAINLVAATFG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
KARIKPGDEIVLSIMEHHANIVPWHFLRESLGAVIKWAPVDEEGNFLMDEFEALLTERTK
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCHH
MVAITQMSNVLGTTVPVKEVVKIAHARGIPVLVDGSQGAVHLDVDVQDLDCDFYIITGHK
EEEEEHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCEEEEECCE
LYGPTGIGALYGKYEHLEAMPPFNGGGEMIREVRQDTVTYGDPPHRFEAGTPAIVQAAGL
EECCCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC
GAALDYIESIGKARIRAHEAELTRYAQERLGELNSVRIIGTAKDKGAIISFEIKGAHAHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCHH
FATLIDNDGIAVRAGTHCAMPLLERFGVSATCRASFGLYNTREEVDRFVAALSKAQELIG
HHHEECCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLPLPAPHPAVSNGSYDVLRVREDFPILDLKVNGKPLVYLDNGASAQKPRQVLDRIQQV
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHH
YTSEYANVHRGLHYLANAATEAYEGGRARVQRFLNAARPEEIVFTRSATEAINLVAATFG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
KARIKPGDEIVLSIMEHHANIVPWHFLRESLGAVIKWAPVDEEGNFLMDEFEALLTERTK
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCHH
MVAITQMSNVLGTTVPVKEVVKIAHARGIPVLVDGSQGAVHLDVDVQDLDCDFYIITGHK
EEEEEHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCEEEEECCE
LYGPTGIGALYGKYEHLEAMPPFNGGGEMIREVRQDTVTYGDPPHRFEAGTPAIVQAAGL
EECCCCHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC
GAALDYIESIGKARIRAHEAELTRYAQERLGELNSVRIIGTAKDKGAIISFEIKGAHAHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCHH
FATLIDNDGIAVRAGTHCAMPLLERFGVSATCRASFGLYNTREEVDRFVAALSKAQELIG
HHHEECCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11251816 [H]