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The map label for this gene is rpoN [H]

Identifier: 154244022

GI number: 154244022

Start: 69302

End: 70864

Strand: Reverse

Name: rpoN [H]

Synonym: Xaut_0064

Alternate gene names: 154244022

Gene position: 70864-69302 (Counterclockwise)

Preceding gene: 154244023

Following gene: 154244019

Centisome position: 1.33

GC content: 68.39

Gene sequence:

>1563_bases
ATGGTGTTGAGCCCCAAGCTGGAATTCCGCCAGAGCCAGTCCCTGGTGATGACCCCGCAGCTGATGCAGGCCATCAAGCT
GCTGCAGCTCTCCAACATGGAGCTGGTGGCCTACGTGGAGGCGGAGCTGGAGCGCAATCCGCTCCTGGAGCGTATCACCG
AAGGCGACGGCCCGGCCGACAGCGCCGGCGGCGAGACCCCCTCCGAGACCGCCCAGCGGGACGACGCCCCCGCCCCCGAG
CCCGAGGCCGCTGTTTCCGGCGACTGGATGCCCGGCGACATGGAGCCCAGCCGGGCGGCCATGGAAACCCGTCTCGACAC
CGATCTCGGCAACGTCTTCCCGGACGACGCCCCCGCCGACCGCGGTGCCGCCGGCGGCGGCTCCACCGGCCAGAGCCCCT
CCACCGGCGAATGGGGCGGCGGCGAGCGCGGCGAGGACTACAATCCCGAAGCCTTCCTCACCGCCGAAATGACCCTGGCC
GACCACCTGGAAGCCCAGCTTGCGGTGGCGATGAGCGATCCGGCGCGACGGCTGATCGGCGTCAACGTCATCGGCCTCAT
CGACGAGACCGGCTATTTCACCGGCGACCTCGACCAGCTGGCGGAGCAACTCGGCGTGGCGCGCGCCGAGGTGGAAGCCA
TGCTGAAGCTCATCCAGACCTTCGAGCCGTCTGGCGTGGGCGCGCGCAACCTCGCCGAATGCCTCGCCATCCAGCTCAGG
GAGCGGGACCGCTTCGACCCCGCCATGCAGGCGCTGGTGGCGAACCTGGAGCTTTTGGCCCGGCACGACCGCAACGCCCT
CAAGCGGGTCTGCGCCGTGGACAACGAGGACCTTGCCGACATGATCGGCGAGATTCGCCGGCTCGATCCCAAACCCGGCC
TCGCCTTCGGCTCCGGCGTGGTGCATCCGCTGGTGCCGGATGTCTATGTGCGGGCGGGCAAGGACGGCGAGTGGCTGGTG
GAGCTGAATTCCGAGACCCTGCCACGGGTGCTGGTGAACCAGACCTATCATGCCACCGTGGCCAAGGTAGCGCGCTCGGC
CGAGGACAAGAGCTTCCTGGCCGAATGCCTCCAGAGCGCCAGCTGGCTGACGCGCTCCCTCGACCAGCGGGCCCGCACCA
TCCTGAAGGTGGCGAGCGAGATCGTGCGCCAGCAGGACGCCTTCCTGCTGCACGGTGTGCGCTATCTGCGGCCCCTGAAC
CTGCGCACGGTGGCCGACGCCATCGGCATGCACGAATCCACCGTCTCGCGAGTCACCTCCAACAAATACATCTCCACCCC
GCGCGGCGTGGTGGAAATGAAGTTCTTCTTCTCGTCGGCCATTTCCGCCTCGGGCGGCGGCGAGGCCCATTCGGCCGAGG
CCGTACGCCACCGCATCAAGCTGCTCATCGAGGCCGAGAGCGCGGAGGACGTGCTCTCCGACGACACCCTGGTGCAGAAG
CTGAAGGACGAGGGCATCGACATCGCCCGCCGCACGGTGGCGAAGTATCGGGAAGGCATGAACATTCCCTCCTCGGTCCA
GCGCCGCCGCGAGAAGATGGCCCTGCGCAGCGAGGCCAGCTGA

Upstream 100 bases:

>100_bases
GTGCGGCGGCTGTATCTCGGCGAGGAGTTCCGGCTATGAGCGCCGCCTCTCCCCGCCTTCCGCTCGCCCGACCCGTGACA
CGCCAGCGGAGGCTTCCGCC

Downstream 100 bases:

>100_bases
GACCAAGAGGCCCCAGCCGGCCCATGCAGAGTCCGTCGATAGGCGCCGCTCCAACGCGCGGCAGCCCTCACGCGGGCTGG
AGTTCGTAGATCTCCCCGTT

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MVLSPKLEFRQSQSLVMTPQLMQAIKLLQLSNMELVAYVEAELERNPLLERITEGDGPADSAGGETPSETAQRDDAPAPE
PEAAVSGDWMPGDMEPSRAAMETRLDTDLGNVFPDDAPADRGAAGGGSTGQSPSTGEWGGGERGEDYNPEAFLTAEMTLA
DHLEAQLAVAMSDPARRLIGVNVIGLIDETGYFTGDLDQLAEQLGVARAEVEAMLKLIQTFEPSGVGARNLAECLAIQLR
ERDRFDPAMQALVANLELLARHDRNALKRVCAVDNEDLADMIGEIRRLDPKPGLAFGSGVVHPLVPDVYVRAGKDGEWLV
ELNSETLPRVLVNQTYHATVAKVARSAEDKSFLAECLQSASWLTRSLDQRARTILKVASEIVRQQDAFLLHGVRYLRPLN
LRTVADAIGMHESTVSRVTSNKYISTPRGVVEMKFFFSSAISASGGGEAHSAEAVRHRIKLLIEAESAEDVLSDDTLVQK
LKDEGIDIARRTVAKYREGMNIPSSVQRRREKMALRSEAS

Sequences:

>Translated_520_residues
MVLSPKLEFRQSQSLVMTPQLMQAIKLLQLSNMELVAYVEAELERNPLLERITEGDGPADSAGGETPSETAQRDDAPAPE
PEAAVSGDWMPGDMEPSRAAMETRLDTDLGNVFPDDAPADRGAAGGGSTGQSPSTGEWGGGERGEDYNPEAFLTAEMTLA
DHLEAQLAVAMSDPARRLIGVNVIGLIDETGYFTGDLDQLAEQLGVARAEVEAMLKLIQTFEPSGVGARNLAECLAIQLR
ERDRFDPAMQALVANLELLARHDRNALKRVCAVDNEDLADMIGEIRRLDPKPGLAFGSGVVHPLVPDVYVRAGKDGEWLV
ELNSETLPRVLVNQTYHATVAKVARSAEDKSFLAECLQSASWLTRSLDQRARTILKVASEIVRQQDAFLLHGVRYLRPLN
LRTVADAIGMHESTVSRVTSNKYISTPRGVVEMKFFFSSAISASGGGEAHSAEAVRHRIKLLIEAESAEDVLSDDTLVQK
LKDEGIDIARRTVAKYREGMNIPSSVQRRREKMALRSEAS
>Mature_520_residues
MVLSPKLEFRQSQSLVMTPQLMQAIKLLQLSNMELVAYVEAELERNPLLERITEGDGPADSAGGETPSETAQRDDAPAPE
PEAAVSGDWMPGDMEPSRAAMETRLDTDLGNVFPDDAPADRGAAGGGSTGQSPSTGEWGGGERGEDYNPEAFLTAEMTLA
DHLEAQLAVAMSDPARRLIGVNVIGLIDETGYFTGDLDQLAEQLGVARAEVEAMLKLIQTFEPSGVGARNLAECLAIQLR
ERDRFDPAMQALVANLELLARHDRNALKRVCAVDNEDLADMIGEIRRLDPKPGLAFGSGVVHPLVPDVYVRAGKDGEWLV
ELNSETLPRVLVNQTYHATVAKVARSAEDKSFLAECLQSASWLTRSLDQRARTILKVASEIVRQQDAFLLHGVRYLRPLN
LRTVADAIGMHESTVSRVTSNKYISTPRGVVEMKFFFSSAISASGGGEAHSAEAVRHRIKLLIEAESAEDVLSDDTLVQK
LKDEGIDIARRTVAKYREGMNIPSSVQRRREKMALRSEAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=511, Percent_Identity=38.5518590998043, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56670; Mature: 56670

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSPKLEFRQSQSLVMTPQLMQAIKLLQLSNMELVAYVEAELERNPLLERITEGDGPAD
CCCCCCCHHHCCCCEEECHHHHHHHHHHHHCCCCEEEHHHHHHHCCHHHHHHHCCCCCCC
SAGGETPSETAQRDDAPAPEPEAAVSGDWMPGDMEPSRAAMETRLDTDLGNVFPDDAPAD
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCC
RGAAGGGSTGQSPSTGEWGGGERGEDYNPEAFLTAEMTLADHLEAQLAVAMSDPARRLIG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHC
VNVIGLIDETGYFTGDLDQLAEQLGVARAEVEAMLKLIQTFEPSGVGARNLAECLAIQLR
CCEEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ERDRFDPAMQALVANLELLARHDRNALKRVCAVDNEDLADMIGEIRRLDPKPGLAFGSGV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
VHPLVPDVYVRAGKDGEWLVELNSETLPRVLVNQTYHATVAKVARSAEDKSFLAECLQSA
CCCCCCCCEEECCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SWLTRSLDQRARTILKVASEIVRQQDAFLLHGVRYLRPLNLRTVADAIGMHESTVSRVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHC
NKYISTPRGVVEMKFFFSSAISASGGGEAHSAEAVRHRIKLLIEAESAEDVLSDDTLVQK
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKDEGIDIARRTVAKYREGMNIPSSVQRRREKMALRSEAS
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLSPKLEFRQSQSLVMTPQLMQAIKLLQLSNMELVAYVEAELERNPLLERITEGDGPAD
CCCCCCCHHHCCCCEEECHHHHHHHHHHHHCCCCEEEHHHHHHHCCHHHHHHHCCCCCCC
SAGGETPSETAQRDDAPAPEPEAAVSGDWMPGDMEPSRAAMETRLDTDLGNVFPDDAPAD
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCC
RGAAGGGSTGQSPSTGEWGGGERGEDYNPEAFLTAEMTLADHLEAQLAVAMSDPARRLIG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHC
VNVIGLIDETGYFTGDLDQLAEQLGVARAEVEAMLKLIQTFEPSGVGARNLAECLAIQLR
CCEEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ERDRFDPAMQALVANLELLARHDRNALKRVCAVDNEDLADMIGEIRRLDPKPGLAFGSGV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
VHPLVPDVYVRAGKDGEWLVELNSETLPRVLVNQTYHATVAKVARSAEDKSFLAECLQSA
CCCCCCCCEEECCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SWLTRSLDQRARTILKVASEIVRQQDAFLLHGVRYLRPLNLRTVADAIGMHESTVSRVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHC
NKYISTPRGVVEMKFFFSSAISASGGGEAHSAEAVRHRIKLLIEAESAEDVLSDDTLVQK
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKDEGIDIARRTVAKYREGMNIPSSVQRRREKMALRSEAS
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8471796 [H]