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The map label for this gene is braE [H]

Identifier: 154243964

GI number: 154243964

Start: 7160

End: 8194

Strand: Direct

Name: braE [H]

Synonym: Xaut_0006

Alternate gene names: 154243964

Gene position: 7160-8194 (Clockwise)

Preceding gene: 154243963

Following gene: 154243965

Centisome position: 0.13

GC content: 65.99

Gene sequence:

>1035_bases
ATGCGCGCCTCCTTCACCCTCATCCTCGGCCTGCCGCTGTTCGTGGCGGCGCTGATCCTGCCGTTCGTGGTCGCCGACAG
CTATGTGGTCCATTCGGCGGCCATGGTGCTCTATTTCGCCTACATGGCCTCGTCGTGGAACTTCGTCTGCGGCTATGTGG
GGCAGGTCTCGCTGGGCCACGCCGCCTTCTCCGGCATCGCCGGCTATGCCAGCGTGCTGCTGTTCACCGGGCTCGGCCTG
TCGCCCTGGATCGGCATGATCCTCGGCGGCCTGCTGGCGGCGGCCATCTCGGTGCCGGTGGGCTATGCCACCTTCCGGCT
GAAGGGGCCCTACTTCACCCTCACCAGCATCGCCTTTGCCGAGATCATGCGCATCTGGCTGGAAAATACCGACGAGTTCC
TCGGCATCCCGCTGAAGGGCGCGCAGGGGCTCACGGTTCCGCCGGTGGGAGGCACCAGCTTTCTCGCTTTCCAGTTCGAC
GGCAAGCTGCCCTATTACTTCATCATCCTCGCCATGCTGGTGGGGGTGATGGCGGCGACCCATGTCATGGAGCGCTCGCG
GCTCGGCTTTTACCTCAAAGCCATCCGCGGCAACCGCGACGGCGCCGAGGCGCTGGGCATCGACCCCACCCGCTACTGCC
TGACCGCGCTGGCGCTCTCCGCCTTCCTCACCGGCCTTGGCGGCGCCTATTACGCGCAGCTGTTCCGCTACATCAATCCG
GAGCGGAACATGGGCATCGACCTGTCCATCGACATGGCGGTGATGACTATCGTGGGCGGGCAGGGCACCCTGCTCGGCCC
GACGCTGGGCGCTTTGGTGCTGCATCCGGTGGGCGAGATGGTGCGCGCCATGCTGGGCGGCCATGTGCTGGGGCTGCACC
TCATCATCTACGGCGTCGTGCTGATGCTGGCGGTGCTGTATTTCCCGAAAGGCATCATCGCCCCGCTCCAGCGCTGGCTG
ACCCCTGCACCTAAAACACCCGCCCCGAAAGCGCCGGCGCACATGGTGACGCCCAAGGAGGTCGAGGGACGATGA

Upstream 100 bases:

>100_bases
CCACCTCGGCGGCGATCTTCTCCTTCGGCCTGTTCATCGTGATCCTGCTCATGCGGCCTGCGGGCCTCATGGGCCGAGCT
TGAGAAGGCACGGGTTCTCC

Downstream 100 bases:

>100_bases
GCACCCGCATCCTCGAGGTGGACGGCATCTCCAAGCGCTTCGGCGGCCTGCAGGCGGTCTCGCCGCTCTCCTTCCACATG
GAGCCGGGCGAGATCCTCGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRASFTLILGLPLFVAALILPFVVADSYVVHSAAMVLYFAYMASSWNFVCGYVGQVSLGHAAFSGIAGYASVLLFTGLGL
SPWIGMILGGLLAAAISVPVGYATFRLKGPYFTLTSIAFAEIMRIWLENTDEFLGIPLKGAQGLTVPPVGGTSFLAFQFD
GKLPYYFIILAMLVGVMAATHVMERSRLGFYLKAIRGNRDGAEALGIDPTRYCLTALALSAFLTGLGGAYYAQLFRYINP
ERNMGIDLSIDMAVMTIVGGQGTLLGPTLGALVLHPVGEMVRAMLGGHVLGLHLIIYGVVLMLAVLYFPKGIIAPLQRWL
TPAPKTPAPKAPAHMVTPKEVEGR

Sequences:

>Translated_344_residues
MRASFTLILGLPLFVAALILPFVVADSYVVHSAAMVLYFAYMASSWNFVCGYVGQVSLGHAAFSGIAGYASVLLFTGLGL
SPWIGMILGGLLAAAISVPVGYATFRLKGPYFTLTSIAFAEIMRIWLENTDEFLGIPLKGAQGLTVPPVGGTSFLAFQFD
GKLPYYFIILAMLVGVMAATHVMERSRLGFYLKAIRGNRDGAEALGIDPTRYCLTALALSAFLTGLGGAYYAQLFRYINP
ERNMGIDLSIDMAVMTIVGGQGTLLGPTLGALVLHPVGEMVRAMLGGHVLGLHLIIYGVVLMLAVLYFPKGIIAPLQRWL
TPAPKTPAPKAPAHMVTPKEVEGR
>Mature_344_residues
MRASFTLILGLPLFVAALILPFVVADSYVVHSAAMVLYFAYMASSWNFVCGYVGQVSLGHAAFSGIAGYASVLLFTGLGL
SPWIGMILGGLLAAAISVPVGYATFRLKGPYFTLTSIAFAEIMRIWLENTDEFLGIPLKGAQGLTVPPVGGTSFLAFQFD
GKLPYYFIILAMLVGVMAATHVMERSRLGFYLKAIRGNRDGAEALGIDPTRYCLTALALSAFLTGLGGAYYAQLFRYINP
ERNMGIDLSIDMAVMTIVGGQGTLLGPTLGALVLHPVGEMVRAMLGGHVLGLHLIIYGVVLMLAVLYFPKGIIAPLQRWL
TPAPKTPAPKAPAHMVTPKEVEGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=316, Percent_Identity=26.2658227848101, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36768; Mature: 36768

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASFTLILGLPLFVAALILPFVVADSYVVHSAAMVLYFAYMASSWNFVCGYVGQVSLGH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHH
AAFSGIAGYASVLLFTGLGLSPWIGMILGGLLAAAISVPVGYATFRLKGPYFTLTSIAFA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
EIMRIWLENTDEFLGIPLKGAQGLTVPPVGGTSFLAFQFDGKLPYYFIILAMLVGVMAAT
HHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
HVMERSRLGFYLKAIRGNRDGAEALGIDPTRYCLTALALSAFLTGLGGAYYAQLFRYINP
HHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
ERNMGIDLSIDMAVMTIVGGQGTLLGPTLGALVLHPVGEMVRAMLGGHVLGLHLIIYGVV
CCCCCCEEEEHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LMLAVLYFPKGIIAPLQRWLTPAPKTPAPKAPAHMVTPKEVEGR
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRASFTLILGLPLFVAALILPFVVADSYVVHSAAMVLYFAYMASSWNFVCGYVGQVSLGH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHH
AAFSGIAGYASVLLFTGLGLSPWIGMILGGLLAAAISVPVGYATFRLKGPYFTLTSIAFA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
EIMRIWLENTDEFLGIPLKGAQGLTVPPVGGTSFLAFQFDGKLPYYFIILAMLVGVMAAT
HHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
HVMERSRLGFYLKAIRGNRDGAEALGIDPTRYCLTALALSAFLTGLGGAYYAQLFRYINP
HHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
ERNMGIDLSIDMAVMTIVGGQGTLLGPTLGALVLHPVGEMVRAMLGGHVLGLHLIIYGVV
CCCCCCEEEEHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LMLAVLYFPKGIIAPLQRWLTPAPKTPAPKAPAHMVTPKEVEGR
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]