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The map label for this gene is yfiF [H]

Identifier: 153950911

GI number: 153950911

Start: 3604936

End: 3606123

Strand: Reverse

Name: yfiF [H]

Synonym: YpsIP31758_3203

Alternate gene names: 153950911

Gene position: 3606123-3604936 (Counterclockwise)

Preceding gene: 153947726

Following gene: 153948960

Centisome position: 76.35

GC content: 53.11

Gene sequence:

>1188_bases
ATGAACGATTCATTTAGTGGCAAAAACGGCAAAGTTAAAGTGATGTACGTCCGTAGTGACGAAAGCAGCGACGACCGTAA
CAGCAGTAACCGCGATAACCGCAACCGCGATAACAGTAATCGTGATAACAGTAATCGTGATAACAGTAATCGTGATAATA
ACAGCCGCGGCAGAAGCCAGCGTCCAGCGGGTAACGGTCGTCCGGGTGATAATGCACGTCAGGGCGATAATACACGTCAG
GGTAATGCACCACGCAATAATGACTCACGTCGTCCTGATTCTGGCCGTAATGAACGTGATCGTCCTAGCCGTCCGGCACG
TTCTGAAGGCAGTAGCCCTTACGATTCACCGTGGAAAACGGTATCTCGTGCCCCTTCGGAAGAGCCAGAGTTTGATCATG
GTGGTATCAGCGGTAAAAGCCATATCGATCCTGCCCAGTTGCGCCGTCAGCGCGCTGAAGAAACACGTGTTTATGGTGAG
AATGCTTGTAAGGCGCTGTTTGAAAGCCGCCCTGATGCTATCGTCCGGGCTTGGTTTGTGCAATCTGTGACCCCGCGTTT
TCGTGACGCGCTGAAATGGATGGCGGCTAACCGCAAAGCTTATCATGTGGTTGAAGAGGATGAGCTGGCGAAAGCTTCGG
GCACTGAACACCATGGCGGGGTATGTTTCCTGATTAAAAAACGTCAGGGGCTGGATGCAGAAACGTATCTGCAACAGCAA
ACTCAGGCTAAAGATTGTGTACTGGCGCTGGAAGAGGTGGGTAACCCCCATAATCTGGGCGGCATTATGCGTACCTGTGC
GCATTTCGGTATCAACGGCGTGTTGCTGCAAGACCCGGCGATGCTAGAATCTGGCGCGGCAGTCCGCACGGCGGAAGGCG
GAGCAGAGCACATCAAGGCCATCAATGCTGATGATTTCTTATCGGTATTGGATACCTTCCGCAAGGCGGGTTACACCATC
GTGACCACCTCCAGCCATAAAGGTGTCAGCTTATCGAAAGCCGAACTGCCAGCTAAAATGGTATTGGTATTAGGGCAGGA
GAGCGATGGTTTGACGGACAGTGCCTGGCAGCAGGGGGATCTGAGTGTCTCTATCGGCGGTACCGGGCGGGTCGAAAGCC
TGAACGTTTCCGTGGCAACGGGTATCCTGTTGGCGGAATGGTGGCGTCAGAATAGCGCTCAAGGCTAG

Upstream 100 bases:

>100_bases
ATTCGATGAGTACGATAGTTGGTAGCTTTTAGTGCTTGCTTATGGCTAACCGGGCGGACATCAGGTAATCTTCGCGCCCT
GCGCGTTGGTGGAGAAGACA

Downstream 100 bases:

>100_bases
AATGATCGAAGAGTAGAACGAGAGACGTTTTAGAACGGTAAAAATAAAAGGGGGCGCTGATGAGTTCAGGGCCCCCTTTT
TTGTTGACCGCTATTTGGCT

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNRDNSNRDNSNRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQ
GNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGE
NACKALFESRPDAIVRAWFVQSVTPRFRDALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQ
TQAKDCVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTI
VTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Sequences:

>Translated_395_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNRDNSNRDNSNRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQ
GNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGE
NACKALFESRPDAIVRAWFVQSVTPRFRDALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQ
TQAKDCVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTI
VTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG
>Mature_395_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNRDNSNRDNSNRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQ
GNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGE
NACKALFESRPDAIVRAWFVQSVTPRFRDALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQ
TQAKDCVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTI
VTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=395, Percent_Identity=58.7341772151899, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1790623, Length=246, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 43360; Mature: 43360

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNRDNSNRDNSNRDNSNRDNNSRGRSQ
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPAGNGRPGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QSVTPRFRDALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQ
HHCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHH
TQAKDCVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEE
INADDFLSVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGD
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCC
LSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNRDNSNRDNSNRDNSNRDNNSRGRSQ
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPAGNGRPGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QSVTPRFRDALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQ
HHCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHH
TQAKDCVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEE
INADDFLSVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGD
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCC
LSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]