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The map label for this gene is potH [H]

Identifier: 153950903

GI number: 153950903

Start: 2370392

End: 2371258

Strand: Direct

Name: potH [H]

Synonym: YpsIP31758_2057

Alternate gene names: 153950903

Gene position: 2370392-2371258 (Clockwise)

Preceding gene: 153947691

Following gene: 153950339

Centisome position: 50.19

GC content: 53.63

Gene sequence:

>867_bases
ATGAAAAGCTGGCGCAACGATCTGCTTGCCTGGTCGCTGATGTTGAGTGGCCTGGGCACCATTCTGTTATTGATGGGTTC
CACCTTTTATGTCGTGGTGGTGCAGAGCCTCGGTTACTTCAACCTTGAAGGGGAAAGCCATTTTTCGCTGGAGTTCTGGC
GTGACATGTTGCAGGACGAGGTGCTGCATAGCGCCCTATTCTACTCGGTAAAAGTTTCGGTGCTGGGCGCGTTTGGTTCG
GTAATCTTCGCGTATCCGCTGGCGTTATGGCTGCGCAAGCCGCTGATCGCTAAAGAAGGTATTATCGCCGTGCTACGCGC
ACCGATGTTTATTCCCGGTATGGTAGCCGCATTTTTGTTCGTCAATATCATCGCCTACCACGGTGTGATTAACGAGTTGT
TAATGGCACTGGGGGTTATCAACGAACCGCTGCGAATGCAAAACGATGACTTCGGTTGGGGCGTTGTTATTCTGCAAGTC
TGGAAGAACCTGCCGTTTGCCCTGATTCTGGTGGGCGGTGCGGTTAATGCCATCCGCAACGATGTGCTGGACGCCGCCAG
CAACCTCGGGGCCAACCGCCTACGGAGCTTTACCGGTATCGTGGTACCACTCACATTACCTGCCGTGCAGGTGACGCTCA
TTCTGGTCTTTATCGGTGCACTCGGCGATTTCGCCTTTTTCTCGGTCGCCGGGCCGCGCAACACATATTCTCTGGCACGC
CTGATGCAAGCCACCGCGATGGAATACGGTGAGTGGAATAACGCTGCCGTGATCGCCGTCATTATTATGACTACCGCGGC
TATTTTTACGCTGCTGATTGCCACTTTAATCACACCGTTCGCCACCCGTAAAGGAGACGTCAAATGA

Upstream 100 bases:

>100_bases
CGGTACGTCTGCAACTGATTAAAAAACCTCCGGTGGCACTTGGCGGGCGGGCGTGTTTCCGCCTGCCGGAGAACGCTATT
CACTTTTTGGAGCCGGTATC

Downstream 100 bases:

>100_bases
GCCGCACCACCAATGGCCGCCTGGTGGTCACGATTTCGCTGGTTTTTTTCGCCATTGCCAATCTACTGTGGCTGGGGCTA
CCGTTCGGTATGGCGATCCT

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKSWRNDLLAWSLMLSGLGTILLLMGSTFYVVVVQSLGYFNLEGESHFSLEFWRDMLQDEVLHSALFYSVKVSVLGAFGS
VIFAYPLALWLRKPLIAKEGIIAVLRAPMFIPGMVAAFLFVNIIAYHGVINELLMALGVINEPLRMQNDDFGWGVVILQV
WKNLPFALILVGGAVNAIRNDVLDAASNLGANRLRSFTGIVVPLTLPAVQVTLILVFIGALGDFAFFSVAGPRNTYSLAR
LMQATAMEYGEWNNAAVIAVIIMTTAAIFTLLIATLITPFATRKGDVK

Sequences:

>Translated_288_residues
MKSWRNDLLAWSLMLSGLGTILLLMGSTFYVVVVQSLGYFNLEGESHFSLEFWRDMLQDEVLHSALFYSVKVSVLGAFGS
VIFAYPLALWLRKPLIAKEGIIAVLRAPMFIPGMVAAFLFVNIIAYHGVINELLMALGVINEPLRMQNDDFGWGVVILQV
WKNLPFALILVGGAVNAIRNDVLDAASNLGANRLRSFTGIVVPLTLPAVQVTLILVFIGALGDFAFFSVAGPRNTYSLAR
LMQATAMEYGEWNNAAVIAVIIMTTAAIFTLLIATLITPFATRKGDVK
>Mature_288_residues
MKSWRNDLLAWSLMLSGLGTILLLMGSTFYVVVVQSLGYFNLEGESHFSLEFWRDMLQDEVLHSALFYSVKVSVLGAFGS
VIFAYPLALWLRKPLIAKEGIIAVLRAPMFIPGMVAAFLFVNIIAYHGVINELLMALGVINEPLRMQNDDFGWGVVILQV
WKNLPFALILVGGAVNAIRNDVLDAASNLGANRLRSFTGIVVPLTLPAVQVTLILVFIGALGDFAFFSVAGPRNTYSLAR
LMQATAMEYGEWNNAAVIAVIIMTTAAIFTLLIATLITPFATRKGDVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=214, Percent_Identity=23.8317757009346, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787080, Length=210, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31533; Mature: 31533

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]