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The map label for this gene is pth [H]

Identifier: 153950891

GI number: 153950891

Start: 2386876

End: 2387466

Strand: Direct

Name: pth [H]

Synonym: YpsIP31758_2074

Alternate gene names: 153950891

Gene position: 2386876-2387466 (Clockwise)

Preceding gene: 153947474

Following gene: 153947042

Centisome position: 50.53

GC content: 47.04

Gene sequence:

>591_bases
GTGAGTAGCATTAAATTAATCGTTGGGCTGGCAAATCCGGGCGCAGAATATGCCCAAACCCGCCATAATGCGGGAGCCTG
GTATGTAGATTTATTGGCTGAACGCCACAATCAGTCCCTGAAAGAAGAGAGTAAATTCTTTGGTTATACCGCACGGTTAA
ATCTGGCAGGCCAAGATATCCGTTTATTGGTACCCGCCACGTTTATGAATCTCAGTGGTAAAGCCGTCGCGGCAATGGCC
AGTTTTTACCGCATTCTGCCGGAAGAGATTCTAGTCGCCCACGATGAGCTGGATATTTTACCGGGGATGGCAAAATTAAA
ACTGGGAGGAGGCAACGGCGGCCATAATGGTCTGAAAGACATTCAGAACAAATTAGGTAATAACCCTAATTTTTACCGCT
TACGTATCGGTATTGGTCATCCTGGTGATAAAAGCAAAGTCACTGGCTTTGTTTTGGGTAAACCCCCCGCTAGCGAACAA
ACGTTGATAGACAATGCCATTGACGAGTCTATCCGCTGCACTGAAGTGCTACTCAATGAAGGCATGACCAAAGCAATGAA
CCGCCTGCACGCTTTTAAGGCCAGCGCATAA

Upstream 100 bases:

>100_bases
TGTATTCCAGTATGAACAAAATGTACTCGAGTTTTGTTATTAGGCACCATGACAGGTAAACTGTGGCACTTTGATTCCTT
TAGTCATAGCGAAAGACAAC

Downstream 100 bases:

>100_bases
CATCATTTATCGAGTCATCCTCTGAGGCGTGCCATTACGCACGCGGCGAAAGAGCATGATCGTGATAAATCATCATTATG
TGTATAATGGCGGCATAAAT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSSIKLIVGLANPGAEYAQTRHNAGAWYVDLLAERHNQSLKEESKFFGYTARLNLAGQDIRLLVPATFMNLSGKAVAAMA
SFYRILPEEILVAHDELDILPGMAKLKLGGGNGGHNGLKDIQNKLGNNPNFYRLRIGIGHPGDKSKVTGFVLGKPPASEQ
TLIDNAIDESIRCTEVLLNEGMTKAMNRLHAFKASA

Sequences:

>Translated_196_residues
MSSIKLIVGLANPGAEYAQTRHNAGAWYVDLLAERHNQSLKEESKFFGYTARLNLAGQDIRLLVPATFMNLSGKAVAAMA
SFYRILPEEILVAHDELDILPGMAKLKLGGGNGGHNGLKDIQNKLGNNPNFYRLRIGIGHPGDKSKVTGFVLGKPPASEQ
TLIDNAIDESIRCTEVLLNEGMTKAMNRLHAFKASA
>Mature_195_residues
SSIKLIVGLANPGAEYAQTRHNAGAWYVDLLAERHNQSLKEESKFFGYTARLNLAGQDIRLLVPATFMNLSGKAVAAMAS
FYRILPEEILVAHDELDILPGMAKLKLGGGNGGHNGLKDIQNKLGNNPNFYRLRIGIGHPGDKSKVTGFVLGKPPASEQT
LIDNAIDESIRCTEVLLNEGMTKAMNRLHAFKASA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=176, Percent_Identity=34.6590909090909, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787455, Length=193, Percent_Identity=78.2383419689119, Blast_Score=299, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6321983, Length=176, Percent_Identity=29.5454545454545, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21271; Mature: 21140

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIKLIVGLANPGAEYAQTRHNAGAWYVDLLAERHNQSLKEESKFFGYTARLNLAGQDI
CCCEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCE
RLLVPATFMNLSGKAVAAMASFYRILPEEILVAHDELDILPGMAKLKLGGGNGGHNGLKD
EEEEEEHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCEEEEECCCCCCCCHHHH
IQNKLGNNPNFYRLRIGIGHPGDKSKVTGFVLGKPPASEQTLIDNAIDESIRCTEVLLNE
HHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHC
GMTKAMNRLHAFKASA
CHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSIKLIVGLANPGAEYAQTRHNAGAWYVDLLAERHNQSLKEESKFFGYTARLNLAGQDI
CCEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCE
RLLVPATFMNLSGKAVAAMASFYRILPEEILVAHDELDILPGMAKLKLGGGNGGHNGLKD
EEEEEEHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCEEEEECCCCCCCCHHHH
IQNKLGNNPNFYRLRIGIGHPGDKSKVTGFVLGKPPASEQTLIDNAIDESIRCTEVLLNE
HHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHC
GMTKAMNRLHAFKASA
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]