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The map label for this gene is pelW [H]

Identifier: 153950791

GI number: 153950791

Start: 1966139

End: 1967806

Strand: Reverse

Name: pelW [H]

Synonym: YpsIP31758_1692

Alternate gene names: 153950791

Gene position: 1967806-1966139 (Counterclockwise)

Preceding gene: 153950191

Following gene: 153948947

Centisome position: 41.66

GC content: 47.54

Gene sequence:

>1668_bases
ATGGGTTTTACAGGCAACATTAAAGGCAGCGACGCAATGATGATTAACTGGCTCAGCGCTATCCGCAGCTATGTTGATTT
AGTCCAGTCTGTTGGTCACAGTCAGCTTAATCCATCCCCATTGCTAGCCGATGGTTTTGATGTATTGACTCACCAACCTG
TTGTTTGGGAATTCCCTGACGGCCACCATACTCCGATATCTAACTTCGCCAGCCAGCAAAACTGGTTACGGACATTGGAT
GCCCTCAGCTTGGTGACTCAGGATCCGCAATATCACCAGCAAGCCCGGGTACAAAGCGGCTATTTTATGCAGCATGGTGT
CCATAACGAAAGTGGGCTTTTTTACTGGGGTGGGCATCGTTTCCTCAATCTGGATACCCTAAAAACTGAAGGGCCAGCCT
CAAAAGACCAGGTACATGAGCTAAAACATCATCTGCCCTATTACGATCTACTGGTAACCATTGATCGGGAGAGGACCCTT
AATTTCTTACAAGGATTTTGGCACGCTCACGTAGAAGATTGGAAAACCCTGGATCTGGGCCGCCATGGCAATTACAGCAA
GCAACGTGACCCACAGGTTTTTACCCATCCTCGATATGATGTGGTTAACCCAGCAGAGTTACCAAAACTGCCTGAAACCA
AAGGACTCACCTTTGTTAACGCGGGCACTGACTTAATTTACGCAGCCTATAAATATGCTGAATATACCGGCGATGCTGCG
GCTGCGGCATGGGGAAAACATCTGTACCGCCAGTATGTTTTAGCGCGTAATCCTGAAACAGGTTTACCGGTCTATCAGTT
CAGTTCACCACAACAACGCCAGCCAATACCCGCTGACGATAACCAGACTCAATCTTGGTATGGCGACCGTGCCAAGCGCC
AATTTGGCCCAGAGTTTGGTGAAATTGCCCGTGAAGCCAATGTGTTGTTCCGCGATATGCGCCCATTGTTGATAGACAAT
CCACTCGCCATGCTGGATATCTTGCGCCAGCAGCCTGATGCAGAGGTCTTGCAGTGGGTGATTGATGGTCTCAAGAATTA
CTATCGCTTTGCTTATGATGTCGAGAGCAATACATTGCGCCCATTATGGAACGATGGCCAAGATATGAGTGGCTACGTCT
TACCGCGTGATGGCTATTATGGCGTCAAGGGCACTGTTATCTCACCATTCCCATTAGACGTCGATTATTTGTTACCGCTG
GTACGTGCCTGGCGGTTAAGTGAAGATGAAGAGTTACTGGATTTAATTGGCGTGTTATTGCTCCGCTGGCAATTAGCTGA
GCTGAATAAAACACAGCGGCGCGCAACACTGATGGCCGCTCAACGCCCTATTGCCTCTCCCTACCTGCTACTGGCTCTAG
TCGAACTGGCTGAGCATTGTCAATGCCCTACGTTGTTTACCTTGGCATGGCAAATAGGTGATGACCTCTTTAAACGACAT
TACCACCGCGGACTGTTTGTAGAATCGGCTCAGCACCGCTATTTCCGTATCGACAATCCGATTGCATTAGCATTATTAAC
CTTGATTGCCGCTAAACAAGATAAGTTAGCAGCAATACCTCAATTTATTACTAACGGTGGTTATATCCATGGAGATTATC
GGGTAAATGGGGAAAGCCGAACTCTGTATGACATCGATTTTATTTATCCGACACTTTTGAATCAGTAG

Upstream 100 bases:

>100_bases
TCGCCAGTAAATACAAATATAATAGATTGAAACGATGTTTCTATTTATAAGGAACCTAAAGCCGGTTCCGTCCTGACGTT
TCGAAGGTGGGGTTACGACG

Downstream 100 bases:

>100_bases
TTTTATTTATCTTTCATTTTATTAAAAATAGGTAAGCATTATGAATGAAAACAGAATGCTGGGGTTAGCCTATATATCAC
CTTATATAATAGGGTTGATA

Product: periplasmic pectate lyase

Products: NA

Alternate protein names: Exopolygalacturonate lyase; ExoPL [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MGFTGNIKGSDAMMINWLSAIRSYVDLVQSVGHSQLNPSPLLADGFDVLTHQPVVWEFPDGHHTPISNFASQQNWLRTLD
ALSLVTQDPQYHQQARVQSGYFMQHGVHNESGLFYWGGHRFLNLDTLKTEGPASKDQVHELKHHLPYYDLLVTIDRERTL
NFLQGFWHAHVEDWKTLDLGRHGNYSKQRDPQVFTHPRYDVVNPAELPKLPETKGLTFVNAGTDLIYAAYKYAEYTGDAA
AAAWGKHLYRQYVLARNPETGLPVYQFSSPQQRQPIPADDNQTQSWYGDRAKRQFGPEFGEIAREANVLFRDMRPLLIDN
PLAMLDILRQQPDAEVLQWVIDGLKNYYRFAYDVESNTLRPLWNDGQDMSGYVLPRDGYYGVKGTVISPFPLDVDYLLPL
VRAWRLSEDEELLDLIGVLLLRWQLAELNKTQRRATLMAAQRPIASPYLLLALVELAEHCQCPTLFTLAWQIGDDLFKRH
YHRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNGGYIHGDYRVNGESRTLYDIDFIYPTLLNQ

Sequences:

>Translated_555_residues
MGFTGNIKGSDAMMINWLSAIRSYVDLVQSVGHSQLNPSPLLADGFDVLTHQPVVWEFPDGHHTPISNFASQQNWLRTLD
ALSLVTQDPQYHQQARVQSGYFMQHGVHNESGLFYWGGHRFLNLDTLKTEGPASKDQVHELKHHLPYYDLLVTIDRERTL
NFLQGFWHAHVEDWKTLDLGRHGNYSKQRDPQVFTHPRYDVVNPAELPKLPETKGLTFVNAGTDLIYAAYKYAEYTGDAA
AAAWGKHLYRQYVLARNPETGLPVYQFSSPQQRQPIPADDNQTQSWYGDRAKRQFGPEFGEIAREANVLFRDMRPLLIDN
PLAMLDILRQQPDAEVLQWVIDGLKNYYRFAYDVESNTLRPLWNDGQDMSGYVLPRDGYYGVKGTVISPFPLDVDYLLPL
VRAWRLSEDEELLDLIGVLLLRWQLAELNKTQRRATLMAAQRPIASPYLLLALVELAEHCQCPTLFTLAWQIGDDLFKRH
YHRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNGGYIHGDYRVNGESRTLYDIDFIYPTLLNQ
>Mature_554_residues
GFTGNIKGSDAMMINWLSAIRSYVDLVQSVGHSQLNPSPLLADGFDVLTHQPVVWEFPDGHHTPISNFASQQNWLRTLDA
LSLVTQDPQYHQQARVQSGYFMQHGVHNESGLFYWGGHRFLNLDTLKTEGPASKDQVHELKHHLPYYDLLVTIDRERTLN
FLQGFWHAHVEDWKTLDLGRHGNYSKQRDPQVFTHPRYDVVNPAELPKLPETKGLTFVNAGTDLIYAAYKYAEYTGDAAA
AAWGKHLYRQYVLARNPETGLPVYQFSSPQQRQPIPADDNQTQSWYGDRAKRQFGPEFGEIAREANVLFRDMRPLLIDNP
LAMLDILRQQPDAEVLQWVIDGLKNYYRFAYDVESNTLRPLWNDGQDMSGYVLPRDGYYGVKGTVISPFPLDVDYLLPLV
RAWRLSEDEELLDLIGVLLLRWQLAELNKTQRRATLMAAQRPIASPYLLLALVELAEHCQCPTLFTLAWQIGDDLFKRHY
HRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNGGYIHGDYRVNGESRTLYDIDFIYPTLLNQ

Specific function: Catalyzes the formation of unsaturated digalacturonates from polygalacturonate or short oligogalacturonates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010702 [H]

Pfam domain/function: PF06917 Pectate_lyase_2 [H]

EC number: =4.2.2.9 [H]

Molecular weight: Translated: 63686; Mature: 63555

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFTGNIKGSDAMMINWLSAIRSYVDLVQSVGHSQLNPSPLLADGFDVLTHQPVVWEFPD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEECCC
GHHTPISNFASQQNWLRTLDALSLVTQDPQYHQQARVQSGYFMQHGVHNESGLFYWGGHR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCE
FLNLDTLKTEGPASKDQVHELKHHLPYYDLLVTIDRERTLNFLQGFWHAHVEDWKTLDLG
EEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEECC
RHGNYSKQRDPQVFTHPRYDVVNPAELPKLPETKGLTFVNAGTDLIYAAYKYAEYTGDAA
CCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHH
AAAWGKHLYRQYVLARNPETGLPVYQFSSPQQRQPIPADDNQTQSWYGDRAKRQFGPEFG
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
EIAREANVLFRDMRPLLIDNPLAMLDILRQQPDAEVLQWVIDGLKNYYRFAYDVESNTLR
HHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
PLWNDGQDMSGYVLPRDGYYGVKGTVISPFPLDVDYLLPLVRAWRLSEDEELLDLIGVLL
CCCCCCCCCCCEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LRWQLAELNKTQRRATLMAAQRPIASPYLLLALVELAEHCQCPTLFTLAWQIGDDLFKRH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YHRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNGGYIHGDYRVNGESR
HHCCCEEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCC
TLYDIDFIYPTLLNQ
EEEEEHHHHHHHHCC
>Mature Secondary Structure 
GFTGNIKGSDAMMINWLSAIRSYVDLVQSVGHSQLNPSPLLADGFDVLTHQPVVWEFPD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEECCC
GHHTPISNFASQQNWLRTLDALSLVTQDPQYHQQARVQSGYFMQHGVHNESGLFYWGGHR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCE
FLNLDTLKTEGPASKDQVHELKHHLPYYDLLVTIDRERTLNFLQGFWHAHVEDWKTLDLG
EEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEECC
RHGNYSKQRDPQVFTHPRYDVVNPAELPKLPETKGLTFVNAGTDLIYAAYKYAEYTGDAA
CCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHH
AAAWGKHLYRQYVLARNPETGLPVYQFSSPQQRQPIPADDNQTQSWYGDRAKRQFGPEFG
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
EIAREANVLFRDMRPLLIDNPLAMLDILRQQPDAEVLQWVIDGLKNYYRFAYDVESNTLR
HHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
PLWNDGQDMSGYVLPRDGYYGVKGTVISPFPLDVDYLLPLVRAWRLSEDEELLDLIGVLL
CCCCCCCCCCCEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LRWQLAELNKTQRRATLMAAQRPIASPYLLLALVELAEHCQCPTLFTLAWQIGDDLFKRH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YHRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNGGYIHGDYRVNGESR
HHCCCEEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCC
TLYDIDFIYPTLLNQ
EEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1766386; 10383957 [H]