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The map label for this gene is galR [H]

Identifier: 153950697

GI number: 153950697

Start: 2149429

End: 2150445

Strand: Direct

Name: galR [H]

Synonym: YpsIP31758_1855

Alternate gene names: 153950697

Gene position: 2149429-2150445 (Clockwise)

Preceding gene: 153947139

Following gene: 153947593

Centisome position: 45.51

GC content: 49.26

Gene sequence:

>1017_bases
GTGGCCACAATGAAAGATGTTGCGCTGAGGGCTGGGGTTTCAGTAGCAACGGTCAGTCGTGTTCTTAATAATACGGCCTA
TGTAGAGCCTGGTACCCGTGAACGTGTAGAGAAGGCAATGCGTGAGTACAACTACCAGCGAAATGCGGCAGCTCTCGCGT
TGGCTAAAAGAAGTGGAAACATGCTGGGCTTACTTACCGGTAATCTGGCTGATCCATTCTTCTCTCGTTTAGCCCGAGGG
GTTGAAGAGGTTGCTCGTAAAAAAGGAGCAAAATTGATGGTCTGCAGTGGGGGCCATCAGGAAGAACTGGAAAAAGCAGG
GCTCGATTTTTTGATCAATCAAGGATGCGAAGCCATCGTTGCGCACATCACACGAATGAGCGAGGCTGAAATCTTGCGCT
ATGCTGCTCACACTCCTGGGCTGGTACTCATTAATCGTTATTTACCCGCTATCGCTAACCGGTGTGTCTGGTTAGACAAT
ACCCGTGCCGCAGAAGCCTCCACCCATTATCTACTTCAGAATGGTCATACCCGAATTGCCTGTGTCACAGCCAAGTTATC
GATTGATGACAAAAAACAGCGCTTGGAAGGGTACCGAGCCGCCATGGCCAGCGCGGGGATTACGGTGCCTGCTGATTGGA
TTATTAGTGTTCCGTTTGATGAGGAGGGGGGCGAATTAGCGGCAGAGAAACTATTAGCCAGTGATCAGAACTTTACCGCA
GTGGTGACATTTAACGATGTGATGGCTGCCGGGATGATGCGGTCATTACATCAAAGAAACATCAGCCTGCCAGAACAACT
TTCAATTGTTGGTTTTGATGATGTCGTATTGGCCCGCTATCTCTATCCATCATTAACTACCGTCCATTACCCGATTGAGC
GAATGGCCAGAAGGGCTGCAAACATCGCTTTGCTGTTACATGCTCAGGGGGAAGTCTCTCCTCAAATCAATATGTTCTCC
GCTGACCTGGTTATCAGGGATTCAGTTGCACAGGTAGGGAAGAATAGGGGGAAATAG

Upstream 100 bases:

>100_bases
CTGTACACATACATTCAATGGTGTTGTGGTGACATGAGTTGCTATCGATGTTCGGTGGTTATAAAACAGCTAAAGAGAGT
GCCTAAATGGGGAGTGACGT

Downstream 100 bases:

>100_bases
GGCAATCAATAGCTAAAAACGTGACTCTCATCACATTTTTATTTCTCATGAAACCGCTTACATGAACAGCCATCCGCGCT
CACATCCTCGGTTCAATATA

Product: LacI family sugar-binding transcriptional regulator

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MATMKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARG
VEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDN
TRAAEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTA
VVTFNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFS
ADLVIRDSVAQVGKNRGK

Sequences:

>Translated_338_residues
MATMKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARG
VEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDN
TRAAEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTA
VVTFNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFS
ADLVIRDSVAQVGKNRGK
>Mature_337_residues
ATMKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARGV
EEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNT
RAAEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAV
VTFNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFSA
DLVIRDSVAQVGKNRGK

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=43.3333333333333, Blast_Score=257, Evalue=7e-70,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=41.6918429003021, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=34.3373493975904, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790194, Length=310, Percent_Identity=34.1935483870968, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=33.8414634146341, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=29.8192771084337, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787906, Length=290, Percent_Identity=29.6551724137931, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=27.4143302180685, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=26.1764705882353, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=27.3584905660377, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=28.7833827893175, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790715, Length=310, Percent_Identity=26.4516129032258, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786268, Length=282, Percent_Identity=24.822695035461, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790689, Length=324, Percent_Identity=25.3086419753086, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36996; Mature: 36865

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGN
CCCHHHHHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCHHHHHEECCCCC
MLGLLTGNLADPFFSRLARGVEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHCCHHHHH
AHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRAAEASTHYLLQNGHTRIA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEE
CVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTA
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEE
VVTFNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAA
EEEEHHHHHHHHHHHHHHCCCCCCHHEEEECCHHHHHHHHHCCCHHHEECCHHHHHHHHC
NIALLLHAQGEVSPQINMFSADLVIRDSVAQVGKNRGK
CEEEEEEECCCCCCCEEEEEECEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
ATMKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGN
CCHHHHHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCHHHHHEECCCCC
MLGLLTGNLADPFFSRLARGVEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHCCHHHHH
AHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRAAEASTHYLLQNGHTRIA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEE
CVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTA
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEE
VVTFNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAA
EEEEHHHHHHHHHHHHHHCCCCCCHHEEEECCHHHHHHHHHCCCHHHEECCHHHHHHHHC
NIALLLHAQGEVSPQINMFSADLVIRDSVAQVGKNRGK
CEEEEEEECCCCCCCEEEEEECEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]