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The map label for this gene is braD [H]

Identifier: 153950567

GI number: 153950567

Start: 3157444

End: 3158991

Strand: Reverse

Name: braD [H]

Synonym: YpsIP31758_2793

Alternate gene names: 153950567

Gene position: 3158991-3157444 (Counterclockwise)

Preceding gene: 153948969

Following gene: 153950095

Centisome position: 66.88

GC content: 50.39

Gene sequence:

>1548_bases
ATGATAGGTCTGTTACTGGGCTTATCGATGTCGGCAATGGCAGGGCCAGCACAGGATTTTGTTGCGGCGAATCGTGCACA
GCAGGCCACATTATTACAACAGTGGGCAACGGAACCTGATGCTAGCCGATTACCTCTTTTGCAGGCGCTAAAACAAGAAA
CAGTAGTGGTAGACAGTGCCGGCCAAGCGTTTATTAAAAATAATCAAAGTATTAATTCGCTGGAAGGCCAATCGCAACCG
ACCGGTACGCTGAAAAAAATCTGGCTCAACAACCGGTTACGTATCTTGATCGCCAATGCCCTGGCGGCACAACGCTTAGT
CAGTGACGATGAAACCGTGCGTTTACAGGCGGCAGTTGAATTACAACGGCAGGCACAGAGCGATCAACTACCGCTGCTGA
ATCAACGCCTGAAGTTGGAAACGGACAGTCAGGTAAAAGAGGCATTGAGCATTGCACTCGCCAATTTACAACTGACGGAC
AGTAATCCGCAGGTGCGTCTGAATGCCGTGCAATTGTTGGGGCAATCAGGTTCACCGCAAACTCAATCACGGCTGCAAAG
TTTAACCGATACGCAGGTTGAACCGAATGAACAGGTCCGAGCCGCAGCAGCCACCAGTTTGAAACAGATACAACATCGGT
TGATGATTGGGGAGGTTATTGGGCAGGTATTTAGCGGACTATCATTGGGTTCAATCTTATTGCTGGCGGCATTGGGGTTA
GCTATCACCTATGGCTTACTGGGGGTGATCAATATGGCGCATGGTGAAATGCTGATGTTGGGAGCCTATTCCGCCTATCT
GGTGCAATCGCTATTCCAACAATTTGCCCCACACTGGCTGGCACTTTATCCATTAATCGCTCTACCGGTTGCGTTCTTTA
TTACTGCGGGCATTGGTATGGCGTTGGAACGCACGGTGATTCGGCATCTGTATGGCCGCCCGTTGGAAACACTGCTAGCG
ACGTGGGGGATCAGCCTGATGTTGATTCAGGGGATCCGCATGTTGTTTGGCCCACAGAATTTGGAAGTTGCTAACCCTGC
ATGGCTGTCCGGTGGGGTTCAGGTTTTACCTAATCTGGTTTTACCGTGGAATCGGATCGCGGTGATTGCATTTGTCATTA
TTGTTTTGCTACTCACATGGTTGCTGCTGAATAAAACCCGCCTTGGTCTGCAAGTCCGTGCAGTGACACAAAATCGGGCA
ATGGCGGCCTGCTGCGGAGTGGCTACGGGGCGGGTTGATATGCTGGCATTCGGTTTGGGTTCTGGTATTGCAGGGCTTGG
CGGTGTAGCACTCTCGCAATTGGGCAATGTCGGCCCGGAATTAGGGCAGGGTTATATCATCGACTCCTTCTTGGTGGTGG
TCCTGGGTGGCGTCGGGCAATTAGCTGGCAGTGTGGTTGCCGCATTAGGGCTGGGGATGTTGAATAAAATTTTGGAACCA
CAAATTGGCGCAGTATTGGGCAAAATCCTGATTTTAGTGCTGATCATTTTATTTATTCAAAAACGACCGCAGGGTCTGTT
TGCCCTTAAGGGCAGGGTGAGTGACTAA

Upstream 100 bases:

>100_bases
TCTGACGTCAGCTCTTTTTCACCACATATGTGTGATGAGTGGGCCTATGAAATCGTTAATTCCTGTTTGGAATAGGGCAA
TCAATTGTGTGTTCAGGCCG

Downstream 100 bases:

>100_bases
TGAATCAACCACTGACGGTAACCTTAACGCAAAAAGCACCGTTCACCAGCCTGTTATTAGGTATGGTCACGCTGATCGCA
CTGATTGTTCTGCCTTTTTT

Product: hydrophobic amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQAFIKNNQSINSLEGQSQP
TGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTD
SNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQVFSGLSLGSILLLAALGL
AITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMALERTVIRHLYGRPLETLLA
TWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRA
MAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVVAALGLGMLNKILEP
QIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD

Sequences:

>Translated_515_residues
MIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQAFIKNNQSINSLEGQSQP
TGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTD
SNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQVFSGLSLGSILLLAALGL
AITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMALERTVIRHLYGRPLETLLA
TWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRA
MAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVVAALGLGMLNKILEP
QIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
>Mature_515_residues
MIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQAFIKNNQSINSLEGQSQP
TGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTD
SNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQVFSGLSLGSILLLAALGL
AITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMALERTVIRHLYGRPLETLLA
TWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRA
MAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVVAALGLGMLNKILEP
QIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=294, Percent_Identity=27.2108843537415, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 55035; Mature: 55035

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSA
CCHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECC
GQAFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVE
CCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEHHHHHH
LQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTDSNPQVRLNAVQLLGQSGSPQ
HHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCEEEHHHHHHCCCCCCH
TQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQVFSGLSLGSILLLAALGL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGM
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ALERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHC
LPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRAMAACCGVATGRVDMLAFGLG
CCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEEHHCCHHHHHHHHCCCCHHHHHHHHC
SGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVVAALGLGMLNKILEP
CCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
QIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSA
CCHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECC
GQAFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVE
CCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEHHHHHH
LQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTDSNPQVRLNAVQLLGQSGSPQ
HHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCEEEHHHHHHCCCCCCH
TQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQVFSGLSLGSILLLAALGL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGM
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ALERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHC
LPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRAMAACCGVATGRVDMLAFGLG
CCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEEHHCCHHHHHHHHCCCCHHHHHHHHC
SGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVVAALGLGMLNKILEP
CCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
QIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]