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The map label for this gene is purT [H]

Identifier: 153950504

GI number: 153950504

Start: 2649793

End: 2650974

Strand: Direct

Name: purT [H]

Synonym: YpsIP31758_2351

Alternate gene names: 153950504

Gene position: 2649793-2650974 (Clockwise)

Preceding gene: 153950161

Following gene: 153949842

Centisome position: 56.1

GC content: 52.88

Gene sequence:

>1182_bases
ATGCTAACCATTGGAACCGCTCTGCGCCCCGGCGCTACCCGTGTCATGTTGCTGGGCGCAGGCGAGCTGGGTAAAGAAGT
GGCGATTGAATGCCAAAGATTAGGGCTTGAGGTGATTGCAGTTGATCGCTATGCCAATGCCCCCGCGATGCATGTTGCTC
ATCGTAGCCACGTCATTAATATGCTGGATGGTGCCGCACTCAAGCAGTTAGTCGCCCAGGAAAAACCGCATTATATCGTG
CCGGAAATCGAAGCTATCGCCACCGATATGCTGGTTGAGCTGGAGAAGATGGGCCAACATGTTGTGCCTTGTGCAGAAGC
CACCCGTCTGACCATGAACCGCGAGGGCATCCGCCGTCTGGCCGCAGAGACGTTGCAATTACCCACGTCCAGTTACCGCT
TTGCCGACACAGATAGCGCCTTCTTTCAGGCCGTGCGCGACATAGGTTACCCTTGCATTGTTAAACCGGTAATGAGTTCT
TCTGGTAAAGGCCAGAGCCTGATCCGCAGCGAGGAACACCTTCAGGCTGCTTGGGAATATGCACAACAAGGTGGCCGTGC
CGGTAGCGGGCGCGTGATTATCGAAGGTTTGGTTCATTTTGATTTTGAAATCACTTTACTGACGATCCGTGCTGTCGATG
GTATTCATTTTTGCGCCCCAATAGGCCACCGTCAGGAAGATGGCGACTATCGCGAGTCCTGGCAACCCCAAGCCATGAGT
GATATCGCCCTGCAGCGAGCAAAAGAGATCTCCGCACAAGTGGTCACCGCGTTAGGTGGGTTTGGGTTATTTGGCGTAGA
ACTGTTTGTTTGTGGTGATGACGTTATTTTCAGCGAAGTTTCTCCTCGCCCACACGATACCGGCATGGTGACCTTGATTT
CGCAGAATATGTCCGAGTTTGCCTTGCACGTGCGCGCATTTCTTGGTTTGCCGATTGGCACTATCCGCCAATATGGGGCG
GCTGCCTCGGCGGTTATTTTGCCAGAACTGACCAGCCAGAATATCACCTATCGTGGGCTGGAAACTGCCCTGATAGGCGA
TACACAGATTCGGTTATTTGGTAAACCTGAAATTGCCGGTAAGCGGCGTTTAGGGGTTGCACTGGCCGTTGCAGATAATA
TAGAAACCGCCATTGAAGTGGCAAAAAAGGCCGCGGGAAACATTGAGGTCAGTGGGGAATAA

Upstream 100 bases:

>100_bases
CCTTTAACATTACAGACGCAATCGTTTTCGTCGCTATTTTGCCGTATACTGGCGCGCGTCAAATTAACGTGTAATCTCCC
ACAGATGTAGGTATCTAACT

Downstream 100 bases:

>100_bases
TCATCCTGCTCATTTAGCCGACGCTAGCAAATAAATAAACCCGCCATTCAGCCAGCGGGTTTATTTTTATCTATCAATTT
TTCTATCTATCGGTCAGTAA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYANAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE

Sequences:

>Translated_393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYANAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
>Mature_393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYANAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=78.0612244897959, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1786733, Length=245, Percent_Identity=24.4897959183673, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=23.1607629427793, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42510; Mature: 42510

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYANAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALKQLVAQEKPHYIVPEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
AAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSSSGKGQSLIRSEEHLQAAWEY
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
HHCCCCCCCCCEEEEEEEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHH
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCEEEEECCCHHHH
ALHVRAFLGLPIGTIRQYGAAASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAG
HHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCEEEECCCEEEEECCCCCCC
KRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
CCCCCEEEEECCCHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYANAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALKQLVAQEKPHYIVPEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
AAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSSSGKGQSLIRSEEHLQAAWEY
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
HHCCCCCCCCCEEEEEEEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHH
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCEEEEECCCHHHH
ALHVRAFLGLPIGTIRQYGAAASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAG
HHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCEEEECCCEEEEECCCCCCC
KRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
CCCCCEEEEECCCHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA