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The map label for this gene is insF1 [C]

Identifier: 153950433

GI number: 153950433

Start: 2389351

End: 2389860

Strand: Direct

Name: insF1 [C]

Synonym: YpsIP31758_2077

Alternate gene names: 153950433

Gene position: 2389351-2389860 (Clockwise)

Preceding gene: 153947546

Following gene: 153947115

Centisome position: 50.59

GC content: 50.98

Gene sequence:

>510_bases
ATGGGGATTAGCCGACAGGCGTGGTATCAGTCTTTGCAGCGGGAACGCGGAAGAGAAATACAGGCCCAAAGTATCGTTGA
ACAGGGAACGGCGTATGTAAGTCTGGTGACCGACGCCTGGTCGAGAAAAATAGTGGGATATCATGTGCATGAAAGCCTGC
ATACCCGGCATGTGGCCGCCGCGCTTAAAATGGCGCTGGTGAGCAGACGCACGACCTCTGCGTTGATACATCACTCCGAC
TGGGGTATCCAGTACTGCTCGCAGGAATATCAGGCGTTGTATCAACGGTATGGTGTCACGTGCTCGATGACGGACGGATA
TGACTGTTATCAGAATGCGCTGGCGGAACGGGTAAACGGGATATTAAAAATGGAGTATCTGCTTGTTAAACCGGAAGATA
TTGTTCAGGCAAGACAGATGGTCAGGGAGTCGGTGGAGATCTACAACACCCGGCGTCCTCATCTGTCGTTAAAATACAAA
ACGCCCGATGAAGTTCATCAGGCGTTTTAA

Upstream 100 bases:

>100_bases
TGAACACGGAGTTCGGAGCCACACTAACAAAAAAGCAGTTGGCCACATTATCACGCAAGCACAAACGCAAGGACTCACAG
TAACGCGCGCGTGCCGGTTC

Downstream 100 bases:

>100_bases
GGCAAAAAAGTGTCAACCTATATCAGGACAGGTCTATATCCGGATCCTCAGCAACCGCAGGAAATCGGTGTACCTTCTTT
CCCCTCGATACCCTCAGTGG

Product: IS3 family transposase orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MGISRQAWYQSLQRERGREIQAQSIVEQGTAYVSLVTDAWSRKIVGYHVHESLHTRHVAAALKMALVSRRTTSALIHHSD
WGIQYCSQEYQALYQRYGVTCSMTDGYDCYQNALAERVNGILKMEYLLVKPEDIVQARQMVRESVEIYNTRRPHLSLKYK
TPDEVHQAF

Sequences:

>Translated_169_residues
MGISRQAWYQSLQRERGREIQAQSIVEQGTAYVSLVTDAWSRKIVGYHVHESLHTRHVAAALKMALVSRRTTSALIHHSD
WGIQYCSQEYQALYQRYGVTCSMTDGYDCYQNALAERVNGILKMEYLLVKPEDIVQARQMVRESVEIYNTRRPHLSLKYK
TPDEVHQAF
>Mature_168_residues
GISRQAWYQSLQRERGREIQAQSIVEQGTAYVSLVTDAWSRKIVGYHVHESLHTRHVAAALKMALVSRRTTSALIHHSDW
GIQYCSQEYQALYQRYGVTCSMTDGYDCYQNALAERVNGILKMEYLLVKPEDIVQARQMVRESVEIYNTRRPHLSLKYKT
PDEVHQAF

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=99, Percent_Identity=40.4040404040404, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1788405, Length=123, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787263, Length=123, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786753, Length=123, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786570, Length=123, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786490, Length=123, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1789981, Length=134, Percent_Identity=32.089552238806, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 19610; Mature: 19478

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISRQAWYQSLQRERGREIQAQSIVEQGTAYVSLVTDAWSRKIVGYHVHESLHTRHVAA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKMALVSRRTTSALIHHSDWGIQYCSQEYQALYQRYGVTCSMTDGYDCYQNALAERVNG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHH
ILKMEYLLVKPEDIVQARQMVRESVEIYNTRRPHLSLKYKTPDEVHQAF
HHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCC
>Mature Secondary Structure 
GISRQAWYQSLQRERGREIQAQSIVEQGTAYVSLVTDAWSRKIVGYHVHESLHTRHVAA
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKMALVSRRTTSALIHHSDWGIQYCSQEYQALYQRYGVTCSMTDGYDCYQNALAERVNG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHH
ILKMEYLLVKPEDIVQARQMVRESVEIYNTRRPHLSLKYKTPDEVHQAF
HHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]