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The map label for this gene is rlmL

Identifier: 153950189

GI number: 153950189

Start: 2887040

End: 2889160

Strand: Reverse

Name: rlmL

Synonym: YpsIP31758_2552

Alternate gene names: 153950189

Gene position: 2889160-2887040 (Counterclockwise)

Preceding gene: 153948536

Following gene: 153946843

Centisome position: 61.17

GC content: 51.2

Gene sequence:

>2121_bases
ATGAACTCTCTGTTTGCCAGCACGGCGCGTGGACTGGAAGAACTGTTAAAAAGCGAACTCGAAGCGCTGGGCGCTCACGA
CTGTAAAATAGTACAGGGTGGGGTACATTTTCAGGGCGACGATCGACTCATGTACCAAAGCCTGTTGTGGAGCCGGTTGG
CTTCGCGCATCCTGTTGCCGCTCAACGAGTTTAAGGTCTACAGTGATTTAGATTTGTACCTTGGGGTACAGGCTATCGAT
TGGCCGTCAATCTTTGGTGTGGACAAAACATTTGCCGTACATTTTAGCGGTGTGAATGACGAGATCCGTAATAGTCAGTA
TGGTGCGTTGAAAGTAAAAGATGCGATTGTGGACAGCTTCACGCGCAAAATGGATCAACGCCCAACGGTTGCCAAACAAC
AGCCTGATATTCGGGTGAACGTTTTCTTGCAACGGGATATGGCCAGCGTGGCTCTTGATCTCAGTGGTGAGGGGCTACAT
CAGCGTGGTTATCGTGATTTGACCGGTCAGGCACCGCTGAAAGAGAATCTGGCGGCAGCGATTATCCAGCGTTCTGGTTG
GCAGCCAGGTACGCCTATGGTCGATCCTATGTGTGGCTCAGGGACTTTGCTGATTGAAGCCGCAATGATGGCCTCTGATC
GTGCACCGGGTTTGCACCGGGGGCATTGGGGTTTTACTGCCTGGAATGCTTTTAATGAAGCGCTGTGGCGTGAACTGACC
ACAGAAGCGCAAGTTCGTGCTCGTCGTGGCCTGCTAGAGACTTCATCTCGCTTCTTTGGTTCAGATATTGATCGTCGGGT
CATCGAAATGGCGCGTGCGAACGCTCGGCGGGCAGGTGTTGCCGAGTTGATTACCTTCAATGCTAACGATATCAGTAAGC
TGGTGAATCCATTACCTGAAGGGCCGGTGGGTACTGTTATCAGCAACCCCCCTTACGGCGAACGGTTGGAAAGTGAGCCT
GCATTGATTGCATTGCATAATATGTTTGGCCGTATGATGAAAACCGCATTTGGCGGCTGGCGTCTGTCACTGTTTAGTGC
TTCTCCAGAATTATTGAGCTGCCTGCAACTGCGAGCGGATCGTGAATTTAAAGCCAAGAATGGCCCGCTGGATTGCGTAC
AGAAAAACTATCAGTTGACGGCGAATCCACAGGGGGCGGGGGGCGCGTTGGTGGCTGAAGATTATGCTAACCGTCTGCGT
AAGAACGTGAAAAAACTGGATAAGTGGGCGAAACAACAGGGTATCGAATGCTACCGTCTGTATGATGCCGACCTACCGGA
TTACAATGTTGCGGTCGATCGCTATGGCAGTAAAGTGGTGGTGCAGGAGTATGCGCCGCCCAAAACGATCGATCCACAAA
AAGCCCGTCAGCGCTTGTTTGATGTGATTAATGCTACGTTGGCGGTGCTAGAACTGCCCTCTAATCAGTTGGTGTTGAAA
ACGCGTGAGCGCCAGAAGGGTAAAAATCAATACGAGAAATTGGCCCAAAAGGGTGAATTCTTGCTGGTCAGTGAGTATAA
CGCCAAGCTATGGGTTAACCTGACTGACTATCTTGATACTGGTCTGTTCCTGGATCACCGTATTGCCCGCCAGATGTTGG
GCAAAATGAGCCAGGGCAAAGATTTCCTTAACCTGTTTGCTTATACCGGTACCGCCAGCGTCCATGCGGGGCTGGGCGGC
GCGCGCAGCACAACCACGGTAGATATGTCGCGTACCTATCTGGAGTGGGCAGAGAAGAACTTGCGAGCGAATGGCTTGAC
GGGCCAGCAACACCGCTTAATTCAGGCGGATTGCCTTTCTTGGCTCAGCAACACTGATGAGCAATTCGACGTGATCTTCA
TTGATCCGCCGACCTTCTCTAACTCCAAGCGTATGGAAACCACCTTTGATGTTCAGCGCGATCATTTAGTGCTGATGAAA
GAACTTAAACGGTTACTGCGTCGCAAGGGGACAATCATGTTCTCGAACAATAAGCGTGGTTTCCAGATGGATCTGGCCGG
GATAGCCGCGCTGGGGCTGGAAGCGAAAGAGATAACCGCACTTACACAATCTGAAGACTTTGCCCGTAATCGTCAGATCC
ACAATTGTTGGCTGGTCACCCACAGCCAAGAGGAAAAGTAA

Upstream 100 bases:

>100_bases
TTACCTCCGTGTGAGTATTAAATTAGAGGGAGCAACTTTATGACAAGCTGCTGGGATTAGCTATAATGCGCAACAATTTT
CTTTTTGGTGATAGTGCGAT

Downstream 100 bases:

>100_bases
AAACTATGTCGTTAATTAGCATGTCTGGAGCCTGGCTGTCGTTCAGCGATGCACCTTTATTAGATAACACTGAATTACAT
ATCGAGGCGAACGAGCGTGT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MNSLFASTARGLEELLKSELEALGAHDCKIVQGGVHFQGDDRLMYQSLLWSRLASRILLPLNEFKVYSDLDLYLGVQAID
WPSIFGVDKTFAVHFSGVNDEIRNSQYGALKVKDAIVDSFTRKMDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKENLAAAIIQRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLHRGHWGFTAWNAFNEALWRELT
TEAQVRARRGLLETSSRFFGSDIDRRVIEMARANARRAGVAELITFNANDISKLVNPLPEGPVGTVISNPPYGERLESEP
ALIALHNMFGRMMKTAFGGWRLSLFSASPELLSCLQLRADREFKAKNGPLDCVQKNYQLTANPQGAGGALVAEDYANRLR
KNVKKLDKWAKQQGIECYRLYDADLPDYNVAVDRYGSKVVVQEYAPPKTIDPQKARQRLFDVINATLAVLELPSNQLVLK
TRERQKGKNQYEKLAQKGEFLLVSEYNAKLWVNLTDYLDTGLFLDHRIARQMLGKMSQGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRANGLTGQQHRLIQADCLSWLSNTDEQFDVIFIDPPTFSNSKRMETTFDVQRDHLVLMK
ELKRLLRRKGTIMFSNNKRGFQMDLAGIAALGLEAKEITALTQSEDFARNRQIHNCWLVTHSQEEK

Sequences:

>Translated_706_residues
MNSLFASTARGLEELLKSELEALGAHDCKIVQGGVHFQGDDRLMYQSLLWSRLASRILLPLNEFKVYSDLDLYLGVQAID
WPSIFGVDKTFAVHFSGVNDEIRNSQYGALKVKDAIVDSFTRKMDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKENLAAAIIQRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLHRGHWGFTAWNAFNEALWRELT
TEAQVRARRGLLETSSRFFGSDIDRRVIEMARANARRAGVAELITFNANDISKLVNPLPEGPVGTVISNPPYGERLESEP
ALIALHNMFGRMMKTAFGGWRLSLFSASPELLSCLQLRADREFKAKNGPLDCVQKNYQLTANPQGAGGALVAEDYANRLR
KNVKKLDKWAKQQGIECYRLYDADLPDYNVAVDRYGSKVVVQEYAPPKTIDPQKARQRLFDVINATLAVLELPSNQLVLK
TRERQKGKNQYEKLAQKGEFLLVSEYNAKLWVNLTDYLDTGLFLDHRIARQMLGKMSQGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRANGLTGQQHRLIQADCLSWLSNTDEQFDVIFIDPPTFSNSKRMETTFDVQRDHLVLMK
ELKRLLRRKGTIMFSNNKRGFQMDLAGIAALGLEAKEITALTQSEDFARNRQIHNCWLVTHSQEEK
>Mature_706_residues
MNSLFASTARGLEELLKSELEALGAHDCKIVQGGVHFQGDDRLMYQSLLWSRLASRILLPLNEFKVYSDLDLYLGVQAID
WPSIFGVDKTFAVHFSGVNDEIRNSQYGALKVKDAIVDSFTRKMDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLH
QRGYRDLTGQAPLKENLAAAIIQRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLHRGHWGFTAWNAFNEALWRELT
TEAQVRARRGLLETSSRFFGSDIDRRVIEMARANARRAGVAELITFNANDISKLVNPLPEGPVGTVISNPPYGERLESEP
ALIALHNMFGRMMKTAFGGWRLSLFSASPELLSCLQLRADREFKAKNGPLDCVQKNYQLTANPQGAGGALVAEDYANRLR
KNVKKLDKWAKQQGIECYRLYDADLPDYNVAVDRYGSKVVVQEYAPPKTIDPQKARQRLFDVINATLAVLELPSNQLVLK
TRERQKGKNQYEKLAQKGEFLLVSEYNAKLWVNLTDYLDTGLFLDHRIARQMLGKMSQGKDFLNLFAYTGTASVHAGLGG
ARSTTTVDMSRTYLEWAEKNLRANGLTGQQHRLIQADCLSWLSNTDEQFDVIFIDPPTFSNSKRMETTFDVQRDHLVLMK
ELKRLLRRKGTIMFSNNKRGFQMDLAGIAALGLEAKEITALTQSEDFARNRQIHNCWLVTHSQEEK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=700, Percent_Identity=75.4285714285714, Blast_Score=1107, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=243, Percent_Identity=27.5720164609054, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_YERP3 (A7FJT9)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401517.1
- ProteinModelPortal:   A7FJT9
- SMR:   A7FJT9
- STRING:   A7FJT9
- GeneID:   5386018
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2552
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   YPSE349747:YPSIP31758_2552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79375; Mature: 79375

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKSELEALGAHDCKIVQGGVHFQGDDRLMYQSLLWSRLASRILLP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCC
LNEFKVYSDLDLYLGVQAIDWPSIFGVDKTFAVHFSGVNDEIRNSQYGALKVKDAIVDSF
HHHHEEECCCHHHCCCEEECCCCCCCCCCEEEEEEECCCHHHCCCCCCEEEHHHHHHHHH
TRKMDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLHQRGYRDLTGQAPLKENLAAA
HHHHCCCCCCCCCCCCCEEEEEEECCHHHEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
IIQRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLHRGHWGFTAWNAFNEALWRELT
HHHHCCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEAQVRARRGLLETSSRFFGSDIDRRVIEMARANARRAGVAELITFNANDISKLVNPLPE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHEEEECCHHHHHHHHCCCC
GPVGTVISNPPYGERLESEPALIALHNMFGRMMKTAFGGWRLSLFSASPELLSCLQLRAD
CCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC
REFKAKNGPLDCVQKNYQLTANPQGAGGALVAEDYANRLRKNVKKLDKWAKQQGIECYRL
CCCCCCCCCHHHHCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPDYNVAVDRYGSKVVVQEYAPPKTIDPQKARQRLFDVINATLAVLELPSNQLVLK
ECCCCCCCCEEHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEE
TRERQKGKNQYEKLAQKGEFLLVSEYNAKLWVNLTDYLDTGLFLDHRIARQMLGKMSQGK
EHHHHCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCC
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAEKNLRANGLTGQQHRLIQADCLS
HHHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
WLSNTDEQFDVIFIDPPTFSNSKRMETTFDVQRDHLVLMKELKRLLRRKGTIMFSNNKRG
HHCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
FQMDLAGIAALGLEAKEITALTQSEDFARNRQIHNCWLVTHSQEEK
EEEHHHHHHHHCCCHHHHHHHHCCHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MNSLFASTARGLEELLKSELEALGAHDCKIVQGGVHFQGDDRLMYQSLLWSRLASRILLP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCC
LNEFKVYSDLDLYLGVQAIDWPSIFGVDKTFAVHFSGVNDEIRNSQYGALKVKDAIVDSF
HHHHEEECCCHHHCCCEEECCCCCCCCCCEEEEEEECCCHHHCCCCCCEEEHHHHHHHHH
TRKMDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLHQRGYRDLTGQAPLKENLAAA
HHHHCCCCCCCCCCCCCEEEEEEECCHHHEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
IIQRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLHRGHWGFTAWNAFNEALWRELT
HHHHCCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEAQVRARRGLLETSSRFFGSDIDRRVIEMARANARRAGVAELITFNANDISKLVNPLPE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHEEEECCHHHHHHHHCCCC
GPVGTVISNPPYGERLESEPALIALHNMFGRMMKTAFGGWRLSLFSASPELLSCLQLRAD
CCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC
REFKAKNGPLDCVQKNYQLTANPQGAGGALVAEDYANRLRKNVKKLDKWAKQQGIECYRL
CCCCCCCCCHHHHCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPDYNVAVDRYGSKVVVQEYAPPKTIDPQKARQRLFDVINATLAVLELPSNQLVLK
ECCCCCCCCEEHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEE
TRERQKGKNQYEKLAQKGEFLLVSEYNAKLWVNLTDYLDTGLFLDHRIARQMLGKMSQGK
EHHHHCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCC
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAEKNLRANGLTGQQHRLIQADCLS
HHHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
WLSNTDEQFDVIFIDPPTFSNSKRMETTFDVQRDHLVLMKELKRLLRRKGTIMFSNNKRG
HHCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
FQMDLAGIAALGLEAKEITALTQSEDFARNRQIHNCWLVTHSQEEK
EEEHHHHHHHHCCCHHHHHHHHCCHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA