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The map label for this gene is kefB [H]

Identifier: 153950176

GI number: 153950176

Start: 4406173

End: 4407981

Strand: Reverse

Name: kefB [H]

Synonym: YpsIP31758_3930

Alternate gene names: 153950176

Gene position: 4407981-4406173 (Counterclockwise)

Preceding gene: 153950796

Following gene: 153949879

Centisome position: 93.32

GC content: 51.24

Gene sequence:

>1809_bases
ATGGAGGGTACAGGGCTACTTACCGCTGTTCTAGTGTTTTTATTCGCTGCTGTAGTTGCGGTCCCTATCGCCCAACGCTT
AGGTATTGGTGCGGTACTGGGTTACCTGATCGCGGGTATTGCCATTGGCCCGTGGGGGTTAGGATTTATTCGCGATGTTG
ACGAAATTCTACATTTTTCTGAGCTAGGCGTCGTCTTCCTGATGTTTATCATTGGGTTGGAGTTGAATCCCGCAAAACTT
TGGCAATTAAGGCGATCTATTTTTGGTGTCGGTGCCGGTCAAGTCGTGATAACGGCCGCGGTACTGGGTGCATTGCTTTA
TTTTACCCAGTTCGCCTGGCAGGCAGCGGTTATCGGTGGCGTAGGTTTGGCGATGTCGTCTACCGCCATGGCATTGCAAC
TAATGCGGGAAAAGGGGATGAACCGCAATGAAGGCGGCCAGCTCGGTTTTTCCGTTCTACTGTTCCAGGATATGGCGGTG
ATCCCCGCGTTAGCCTTGATTCCTATTCTGGCGGGTAACGAGGGGGGAGCCAATGACTGGGTCAAGATTGGCCTCAAAAT
TGCGGCTTTTGCCGGTATGTTGATTGGGGGCCGTTATTTGTTGCGCCCGTTGTTCCGCTACATTGTCGCCTCTGGGGTAC
GTGAGGTTTTTACCGCTGCGGCATTGCTGGTGGTGCTGGGGTCCGCGCTGTTTATGGATGCCCTGGGGCTATCGATGGCA
TTGGGGACATTTATCGCCGGGATATTACTGGCTGAAAGTGAGTTTCAGCACGAATTGGAAATCGCTATCGAGCCGTTTAA
AGGGCTCTTGCTCGGTTTGTTTTTTATTTCTGTCGGCATGGCATTGGACCTCGGCGTGCTGTTTACCCATTTACTGGATG
TACTGCTGGGCGTCTTGGCACTGGTCTTTATCAAAAGTGCCATCTTGTATGGTTTGGCGCGGGTGTTTGGCTTGCGGCGT
TCGGTCCGTTTGCAATTTGCCGGTGTGCTCAGCCAAGGCGGCGAATTTGCTTTCGTCCTGTTTTCCGCGGCGTTCTCTCA
GCGTGTGTTGAATACAGAACAACTGGCATTGCTATTGGTGGTCGTCACCTTATCGATGATGACTACGCCGTTGCTGATGC
AGGTTATTGACCGTATTTTGGTTCGTCGCTACAACGCGCAGGAAGAGAGTGATGAGAAACCCTTTGTTGAAGATAATGAC
CCACAGGTGATTATTGTCGGCTTCGGGCGATTTGGTCAGGTGATTGGCCGTTTATTGATGGCGAACAAAATGCGCATTAC
CGTACTGGAACGTGATGTCAGCGCGGTCAGTATGATGCGCAAATATGGCTACAAAGTTTACTATGGTGACGCGACCGAGT
TGGAACTGCTACGAGCCGCTGGCGCAGAAAAAGCCAAAGCCATTGTTATCACCTGCAATGAGCCGGAAGACACCATGGCG
CTGGTGCATTTATGCCAGCAACACTTCCCTAATCTGCACATTCTTGCTCGCGCCAGAGGGCGTGTCGAAGCCCATGAACT
GCTCCAAAACGGGGTCAAAGACTTCACGCGTGAAACCTTCTCCAGTGCGCTGGAACTGGGGCGTAAGACATTATTGGAAC
TGGGCATGCATCCACATCAGGCCTACCGGGCGCAACAACATTTTCGTCGCTTGGACATGCGAATGCTACGTGAGCTGATG
CCACAACATCATGGCGATGTGGCACAAATTTCCAGAATTAAAGAAGCTCGCCGTGAGTTGGAAGATATTTTTCAACGAGA
AATGCTACATGAAAGCCGGCAGTTAGATGGGTGGGATGAATACGAATAA

Upstream 100 bases:

>100_bases
TATTTACTGGGCCAGACGCCAAAAGCCGGAAACACTCGCCAGTCACGCACAAGCTTATGTGCAATGGCTGCAGTCACCGC
TCACGAGAGGACTCTGATTT

Downstream 100 bases:

>100_bases
TGTCGAGGACAAACGATGACTATCACTCTTAATAAGACAGTGACACGTAAAAGATTTATTGCCGGAGCCGTCTGCCCGCA
ATGTAATGCCTTGGATACGC

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNTEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE

Sequences:

>Translated_602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNTEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE
>Mature_602_residues
MEGTGLLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKL
WQLRRSIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGNEGGANDWVKIGLKIAAFAGMLIGGRYLLRPLFRYIVASGVREVFTAAALLVVLGSALFMDALGLSMA
LGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRR
SVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNTEQLALLLVVVTLSMMTTPLLMQVIDRILVRRYNAQEESDEKPFVEDND
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVSMMRKYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMA
LVHLCQQHFPNLHILARARGRVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFRRLDMRMLRELM
PQHHGDVAQISRIKEARRELEDIFQREMLHESRQLDGWDEYE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=398, Percent_Identity=27.1356783919598, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789749, Length=602, Percent_Identity=77.9069767441861, Blast_Score=922, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=600, Percent_Identity=43.3333333333333, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786685, Length=545, Percent_Identity=29.9082568807339, Blast_Score=183, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66359; Mature: 66359

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA