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The map label for this gene is ppsA [H]

Identifier: 153950097

GI number: 153950097

Start: 2006178

End: 2008562

Strand: Direct

Name: ppsA [H]

Synonym: YpsIP31758_1737

Alternate gene names: 153950097

Gene position: 2006178-2008562 (Clockwise)

Preceding gene: 153948973

Following gene: 153949009

Centisome position: 42.47

GC content: 50.15

Gene sequence:

>2385_bases
ATGTCCAACAATGGCCACACCCGCTGTAATGTGCTTTGGTACAACCAGCTTGGCATGCACGATGTTGACCGCGTAGGGGG
TAAAAATGCCTCCCTGGGTGAAATGATAACTCATCTTTCTGAACTGGGCGTTTCTGTGCCCAATGGCTTTGCTACCACAG
CTCAGGCGTTTAACGACTTTCTGGAGCAAAGTGGTGTCAACCAACGGATTTATCAATTATTGGATAATACCAATATTGAC
GATATCGCAGAATTATCCAAAGCGGGCGCTCAGATCCGCCAATGGGTTATTGAGACGCCGTTCCATCCAGAGTTCGAACA
ATCAATTTATGACGCTTATAAGCAATTAGCTGACGGCGAACCTGAGGCATCATTTGCTGTTCGCTCTTCTGCGACAGCAG
AAGATATGCCGGATGCTTCCTTTGCTGGGCAACAAGAGACATTTCTTAATGTACAGGGCATTGATGCGATAATGGTCGCA
ATTAAGCACGTGTTCGCTTCACTGTTTAATGACCGGGCTATTTCTTATCGTGTGCATCAGGGGTACGACCACCGCGGCGT
AGCATTGTCTGCCGGCGTACAACGGATGGTGCGTTCTGATCTTGCGTCTTCGGGCGTGATGTTCACGATTGATACCGAGT
CTGGTTTTGATCAAGTGGTCTTTATCACCGCAGCGCATGGTCTGGGAGAAATGGTGGTACAGGGGGCGGTTAACCCTGAT
GAATTCTATGTACACAAACCCACATTGCGTAACGGTAAACCGGCCATCGTGCGGCGCAATATGGGGTCCAAAAAAATTCG
GATGATATACGCCGAGAACCAAGAACATGGCAAGCAGGTGTGTATTGAAGATGTGCCAGAAATACAACGCAATCGTTTCG
CTCTCAGTGATGATGAAGTAGAAGCATTGGCCCATCAGGCGCTGTTGATTGAAGAACATTATGGCCGCCCAATGGATATC
GAGTGGGCCAAAGATGGTCATACGGGGCAGTTGTTTATCGTGCAGGCCCGCCCGGAAACCGTGCGTTCCAATGAGCAGGT
GATGGAACGCTACCAGTTGAATAATCAAAGTAATGTGCTGGTGGAAGGCCGTGCTATCGGTCATCGTATTGGCGCGGGTC
CGGTCAAGGTTATTCATGACATCAGTGAGATGGATCGAATTCAGGCGGGTGATGTCCTGGTGACGGATATGACTGACCCC
GATTGGGAGCCGATCATGAAAAAGGCCTCAGCCATTGTTACCAACCGTGGTGGGCGTACCTGCCATGCCGCGATTATTGC
CCGTGAATTGGGGATACCTGCGGTGGTCGGTTGTGGTAATGCAACCGAGCTATTGAAAGAGGGCCAAAATGTTACCGTCT
CCTGTGCTGAGGGGGATACGGGTTACGTTTATCACGAGCTACTGGACTTCTCGGTTCAAAGTTCGCAAGTCACTGAACTG
CCTGATTTACCGTTAAAAATCATGATGAATGTTGGCAACCCGGACCGGGCTTTTGATTTCGCCCGTTTACCTAATGAAGG
GGTCGGTCTGGCGCGGCTGGAGTTTATTATCAATCGGATGATTGGTGTGCATCCCAAAGCTCTTCTGGAGTTTGATCAGC
AGGAGCCTGCGCTACAGGCTGAAATCAAAGCGTTGATGCATGGCTATGACGACCCGGTCGAGTTCTATATTGGTCGCCTG
ACCGAGGGGATCGCTACATTGGGGGCGGCATTTTGGCCAAAACCGGTGATTGTTCGGTTGTCTGACTTTAAGTCTAATGA
GTATGCCAATCTGGTGGGGGGCGCGGGTTATGAACCGCACGAAGAGAACCCAATGCTAGGTTTCCGTGGCGCGGGGCGCT
ATGTCTCTGATCACTTCCGTGATTGCTTCGCTCTGGAATGTGAGGCGATGAAGCGAGTGCGCAATGTGATGGGGTTGACT
AACGTCGAAGTGATGGTGCCTTTTGTGCGGACGGTGGCGCAAGCGGAGGCCGTAGTGGCCGAACTGGCAAGGCAAGGATT
GAAGCGCGGTGAGAATGGTCTGAAGATCATCATGATGTGTGAAATTCCTTCTAATGCATTGCTGGCAGAACAGTTCCTTG
AGCATTTTGATGGTTTCTCCATTGGTTCCAATGATATGACTCAATTGACACTGGGGCTGGATCGTGATTCTGGCGTGGTA
TCGGCGTTGTTTGATGAGCGCAATGAGGCAGTAAAAGCGTTGTTATCAATGGCAATCCAAGCGGCTAAACGTCACGGTAA
ATATGTCGGGATTTGTGGTCAAGGTCCGTCAGACCATGAAGATTTCGCTCTGTGGTTAATGGAACAGGGGATCGACAGCT
TGTCTCTTAATCCAGATACCGTAGTGAAAACTTGGCTGAGCTTGGCGCAACACACCAAACATTGA

Upstream 100 bases:

>100_bases
CGGCGATGTAATAACTAATCGACGGTGTAATAACTAATCGGCGATGTAATAACTAATCGGCGATGTAATAACTAATCGAC
AATGTAAAAGGATTGTTTCG

Downstream 100 bases:

>100_bases
TCACTCAGGGTTAGGGATGAGTGTTTGTTTGTGAGAGTGAATGTGAGTGTTCATTGAGACGAGTATTTATAGGTATTCAT
CATCCGCAGCTTAGGTTGCG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MSNNGHTRCNVLWYNQLGMHDVDRVGGKNASLGEMITHLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYQLLDNTNID
DIAELSKAGAQIRQWVIETPFHPEFEQSIYDAYKQLADGEPEASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAIMVA
IKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFTIDTESGFDQVVFITAAHGLGEMVVQGAVNPD
EFYVHKPTLRNGKPAIVRRNMGSKKIRMIYAENQEHGKQVCIEDVPEIQRNRFALSDDEVEALAHQALLIEEHYGRPMDI
EWAKDGHTGQLFIVQARPETVRSNEQVMERYQLNNQSNVLVEGRAIGHRIGAGPVKVIHDISEMDRIQAGDVLVTDMTDP
DWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKEGQNVTVSCAEGDTGYVYHELLDFSVQSSQVTEL
PDLPLKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGVHPKALLEFDQQEPALQAEIKALMHGYDDPVEFYIGRL
TEGIATLGAAFWPKPVIVRLSDFKSNEYANLVGGAGYEPHEENPMLGFRGAGRYVSDHFRDCFALECEAMKRVRNVMGLT
NVEVMVPFVRTVAQAEAVVAELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEHFDGFSIGSNDMTQLTLGLDRDSGVV
SALFDERNEAVKALLSMAIQAAKRHGKYVGICGQGPSDHEDFALWLMEQGIDSLSLNPDTVVKTWLSLAQHTKH

Sequences:

>Translated_794_residues
MSNNGHTRCNVLWYNQLGMHDVDRVGGKNASLGEMITHLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYQLLDNTNID
DIAELSKAGAQIRQWVIETPFHPEFEQSIYDAYKQLADGEPEASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAIMVA
IKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFTIDTESGFDQVVFITAAHGLGEMVVQGAVNPD
EFYVHKPTLRNGKPAIVRRNMGSKKIRMIYAENQEHGKQVCIEDVPEIQRNRFALSDDEVEALAHQALLIEEHYGRPMDI
EWAKDGHTGQLFIVQARPETVRSNEQVMERYQLNNQSNVLVEGRAIGHRIGAGPVKVIHDISEMDRIQAGDVLVTDMTDP
DWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKEGQNVTVSCAEGDTGYVYHELLDFSVQSSQVTEL
PDLPLKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGVHPKALLEFDQQEPALQAEIKALMHGYDDPVEFYIGRL
TEGIATLGAAFWPKPVIVRLSDFKSNEYANLVGGAGYEPHEENPMLGFRGAGRYVSDHFRDCFALECEAMKRVRNVMGLT
NVEVMVPFVRTVAQAEAVVAELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEHFDGFSIGSNDMTQLTLGLDRDSGVV
SALFDERNEAVKALLSMAIQAAKRHGKYVGICGQGPSDHEDFALWLMEQGIDSLSLNPDTVVKTWLSLAQHTKH
>Mature_793_residues
SNNGHTRCNVLWYNQLGMHDVDRVGGKNASLGEMITHLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYQLLDNTNIDD
IAELSKAGAQIRQWVIETPFHPEFEQSIYDAYKQLADGEPEASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAIMVAI
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFTIDTESGFDQVVFITAAHGLGEMVVQGAVNPDE
FYVHKPTLRNGKPAIVRRNMGSKKIRMIYAENQEHGKQVCIEDVPEIQRNRFALSDDEVEALAHQALLIEEHYGRPMDIE
WAKDGHTGQLFIVQARPETVRSNEQVMERYQLNNQSNVLVEGRAIGHRIGAGPVKVIHDISEMDRIQAGDVLVTDMTDPD
WEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKEGQNVTVSCAEGDTGYVYHELLDFSVQSSQVTELP
DLPLKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGVHPKALLEFDQQEPALQAEIKALMHGYDDPVEFYIGRLT
EGIATLGAAFWPKPVIVRLSDFKSNEYANLVGGAGYEPHEENPMLGFRGAGRYVSDHFRDCFALECEAMKRVRNVMGLTN
VEVMVPFVRTVAQAEAVVAELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEHFDGFSIGSNDMTQLTLGLDRDSGVVS
ALFDERNEAVKALLSMAIQAAKRHGKYVGICGQGPSDHEDFALWLMEQGIDSLSLNPDTVVKTWLSLAQHTKH

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=793, Percent_Identity=83.6065573770492, Blast_Score=1387, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=430, Percent_Identity=27.4418604651163, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.9842931937173, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789193, Length=405, Percent_Identity=24.9382716049383, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=330, Percent_Identity=26.969696969697, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87697; Mature: 87566

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNGHTRCNVLWYNQLGMHDVDRVGGKNASLGEMITHLSELGVSVPNGFATTAQAFNDF
CCCCCCEEEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LEQSGVNQRIYQLLDNTNIDDIAELSKAGAQIRQWVIETPFHPEFEQSIYDAYKQLADGE
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PEASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAIMVAIKHVFASLFNDRAISYRVHQ
CCCCEEECCCCCCCCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GYDHRGVALSAGVQRMVRSDLASSGVMFTIDTESGFDQVVFITAAHGLGEMVVQGAVNPD
CCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCC
EFYVHKPTLRNGKPAIVRRNMGSKKIRMIYAENQEHGKQVCIEDVPEIQRNRFALSDDEV
CEEEECCCCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHH
EALAHQALLIEEHYGRPMDIEWAKDGHTGQLFIVQARPETVRSNEQVMERYQLNNQSNVL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHCCHHHHHHHHHCCCCCCEE
VEGRAIGHRIGAGPVKVIHDISEMDRIQAGDVLVTDMTDPDWEPIMKKASAIVTNRGGRT
EECHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGIPAVVGCGNATELLKEGQNVTVSCAEGDTGYVYHELLDFSVQSSQVTEL
HHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCC
PDLPLKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGVHPKALLEFDQQEPALQA
CCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHH
EIKALMHGYDDPVEFYIGRLTEGIATLGAAFWPKPVIVRLSDFKSNEYANLVGGAGYEPH
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCC
EENPMLGFRGAGRYVSDHFRDCFALECEAMKRVRNVMGLTNVEVMVPFVRTVAQAEAVVA
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEHFDGFSIGSNDMTQLTLGLDRDSGVV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SALFDERNEAVKALLSMAIQAAKRHGKYVGICGQGPSDHEDFALWLMEQGIDSLSLNPDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHH
VVKTWLSLAQHTKH
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SNNGHTRCNVLWYNQLGMHDVDRVGGKNASLGEMITHLSELGVSVPNGFATTAQAFNDF
CCCCCEEEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LEQSGVNQRIYQLLDNTNIDDIAELSKAGAQIRQWVIETPFHPEFEQSIYDAYKQLADGE
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PEASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAIMVAIKHVFASLFNDRAISYRVHQ
CCCCEEECCCCCCCCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GYDHRGVALSAGVQRMVRSDLASSGVMFTIDTESGFDQVVFITAAHGLGEMVVQGAVNPD
CCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCC
EFYVHKPTLRNGKPAIVRRNMGSKKIRMIYAENQEHGKQVCIEDVPEIQRNRFALSDDEV
CEEEECCCCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHH
EALAHQALLIEEHYGRPMDIEWAKDGHTGQLFIVQARPETVRSNEQVMERYQLNNQSNVL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHCCHHHHHHHHHCCCCCCEE
VEGRAIGHRIGAGPVKVIHDISEMDRIQAGDVLVTDMTDPDWEPIMKKASAIVTNRGGRT
EECHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGIPAVVGCGNATELLKEGQNVTVSCAEGDTGYVYHELLDFSVQSSQVTEL
HHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCC
PDLPLKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGVHPKALLEFDQQEPALQA
CCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHH
EIKALMHGYDDPVEFYIGRLTEGIATLGAAFWPKPVIVRLSDFKSNEYANLVGGAGYEPH
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCC
EENPMLGFRGAGRYVSDHFRDCFALECEAMKRVRNVMGLTNVEVMVPFVRTVAQAEAVVA
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEHFDGFSIGSNDMTQLTLGLDRDSGVV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SALFDERNEAVKALLSMAIQAAKRHGKYVGICGQGPSDHEDFALWLMEQGIDSLSLNPDT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHH
VVKTWLSLAQHTKH
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]