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The map label for this gene is ynfL [H]

Identifier: 153950017

GI number: 153950017

Start: 2166120

End: 2167034

Strand: Reverse

Name: ynfL [H]

Synonym: YpsIP31758_1870

Alternate gene names: 153950017

Gene position: 2167034-2166120 (Counterclockwise)

Preceding gene: 153948178

Following gene: 153950472

Centisome position: 45.88

GC content: 46.56

Gene sequence:

>915_bases
ATGAGTATTGAACTAAGACATTTGCGGTATTTTATTGCTGTCGCGGAAGAGCTGCACTTTGGCCGGGCAGCTGAACGCTT
GCATATTTCTCAACCCCCCCTGAGCCAGCAAATCCAAATCCTTGAAGAACAGATTGGGGCCCGCTTATTGGCACGAAATA
AGCGTAATATCAGTCTCACCCAAGCGGGAAAGCTCTTTTTAAAAGAGTCCTACCAAATTTTGGCACAGGTCAACTCTGCA
ACTGAGAAAGCAGCCTGTCTACATCGTGGGGAGTCGGGAGTGCTGACGATCGGTTTCACTTCATCGGCACCATTGATCAA
TGTCGTTTCTAAGAATTTGCGTACTTTTCGGCAAATATATCCACAAGTACATATAAAAATGCAGGAGGTAAGTACCAAAC
AGCAAATTGAAGCATTACTCGACGGGCGGTTAGATCTAGGTATTATGCGTAATACTCGGCTGCCTGAGGCATTACAATAT
CGATTGCTATTACGTGAACCTCTGGTCGCTGTCGTCCATGAAGATCATCCATTAGCCTCCCTACCAAATGGCAGTGTCAA
ATTTAGCTCACTGGACAAACAACCTTTTGTCTTCTTTTCACGTGAAGTGGGGACTGCACTTTATGACGAATTCTTTACCT
TATTAACCCGCGCGGGGATCACACCTTATATTACGCAGGAGGTCGGCGAGGCGATGACCATCATAGGCTTGGTATCATCT
GGCCTGGGGGTTTCAATCCTGCCCGCCTCGTTTAGGCGAGTAAACGTTGATGGTGTCAAATACTTATCACTGGACGAAGC
TGATGCGACGACAGAAATGTGGTTGGTTAATCATAAGCATCGTCCGATGCCCCCCCCTGCAGAAGCATTGCTCCGTTTGA
TGATGGCTGATATTCAAGTTGCTCCGTATAAATAG

Upstream 100 bases:

>100_bases
TACACAATAAATCATTATCGACAGAACATTTTAATCATAGAAATAGTTTGTTTTTTTGTCTAATATATTAATCATTGCAA
ATAATACGTTTAAAATATCA

Downstream 100 bases:

>100_bases
TCACCATATTAAGGAAAAAAGAAAACACATGATTATGTGCAGCAAATCACATAACTAATCAAATAGTTGACGTCATATAC
CAAAAGCCCCAACATAACGA

Product: LysR family substrate binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKLFLKESYQILAQVNSA
TEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQY
RLLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQEVGEAMTIIGLVSS
GLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPPAEALLRLMMADIQVAPYK

Sequences:

>Translated_304_residues
MSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKLFLKESYQILAQVNSA
TEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQY
RLLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQEVGEAMTIIGLVSS
GLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPPAEALLRLMMADIQVAPYK
>Mature_303_residues
SIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKLFLKESYQILAQVNSAT
EKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQYR
LLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQEVGEAMTIIGLVSSG
LGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPPAEALLRLMMADIQVAPYK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=59.581881533101, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI1788887, Length=259, Percent_Identity=38.2239382239382, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=33.7121212121212, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=30.1038062283737, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=32.6996197718631, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=30.1960784313725, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081904, Length=181, Percent_Identity=35.3591160220994, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=28.90625, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790208, Length=304, Percent_Identity=25.9868421052632, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789506, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=33.0769230769231, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=24.7386759581882, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787589, Length=145, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=27.683615819209, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=240, Percent_Identity=25.8333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=23.1075697211155, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=25.5102040816327, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34078; Mature: 33947

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLT
CCEEHHHHHHHHHHHHHHHHCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHH
QAGKLFLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
PQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQYRLLLREPLVAVVHEDHPLAS
HHHEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCC
LPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQEVGEAMTIIGLVSS
CCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPPAEALLRLMMADIQV
CCCEEEECCCCEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
APYK
CCCC
>Mature Secondary Structure 
SIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLT
CEEHHHHHHHHHHHHHHHHCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHH
QAGKLFLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
PQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQYRLLLREPLVAVVHEDHPLAS
HHHEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCC
LPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQEVGEAMTIIGLVSS
CCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPPAEALLRLMMADIQV
CCCEEEECCCCEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
APYK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]