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The map label for this gene is mdfA [H]

Identifier: 153949984

GI number: 153949984

Start: 4637831

End: 4639060

Strand: Direct

Name: mdfA [H]

Synonym: YpsIP31758_4110

Alternate gene names: 153949984

Gene position: 4637831-4639060 (Clockwise)

Preceding gene: 153947329

Following gene: 153948390

Centisome position: 98.19

GC content: 52.03

Gene sequence:

>1230_bases
ATGCAAACTTCATTTTCACCCGCGACACGCTTAGGTCGACGGGCGCTTTTATTCCCTCTCTGCTTAGTATTGTTCGAATT
TGCAGCGTATATCGCCAACGATATGATCCAGCCAGGTATGCTGGCAGTGGTCGCCGAGTTTAATGCCAGTGTCGAGTGGG
TTCCAACCTCTATGACCGCTTATCTGGCGGGGGGGATGTTCCTACAATGGCTTCTAGGGCCGTTGTCTGACCGCCGTGGG
CGTCGTCCAGTGATGTTGGCTGGGGTCGCTTTCTTTGTCGTGACTTGCCTTGCCATTTTACTGGTTAATAGCATTGAACA
GTTCATCGCAATGCGCTTCTTGCAAGGCATTGGCTTGTGCTTTATCGGTGCCGTCGGTTACGCCACTATTCAGGAATCTT
TTGAAGAAGCGGTCTGTATTAAAATCACCGCGTTAATGGCCAATGTGGCATTGATTGCTCCGTTGCTTGGCCCATTGGCC
GGTGCTGCCCTGATCCATGTGGCTCCCTGGCAAACCATGTTCGTCTTGTTTGCAGTGCTGGGGGCAATTTCATTTGCCGG
CCTGTGGCGCGCGATGCCAGAAACTGCTTCGCTGAAAGGGGAGAAACTGTCAGTTGCCAACATGTGGCGTGATTATAAGC
AAGTACTTGCTAACCGCCGCTTCCTCTGTGGTTCTTTAGCATTGGGCTTTGCCAGCCTGCCTTTGCTGGCATGGATTGCC
CAGTCACCGGTAATTTTGATCAGCGGAGAGCAACTCTCTACTTTTGAATATGGCATTTTGCAGGTACCAATTTTCGGCGC
ACTGATTATAGGTAACCTGACACTGGCACGATTGAGCGGTAAAACCAGCATTCCTCAACTCATCCGTTACGGCGCTGGCC
CAATGATTGTTGGCCTGATGATCGCCGCCGGTTCAACACTCTATTCATCCCATGCCTATCTGTGGATGACCGCCGGGTTG
AGCCTGTATGCTTTTGGTATCGGTCTGGCGAACGCCGGTCTGGTGCGGTTGACACTATTCGCCAGTGATATCAGTAAAGG
AACCGTCTCTGCGGCCATGGGGATGATCAGCATGATGATCTTCACCTTAGGTATTGAGTTGGCCAAAGTGGCTTACCTGT
GGGGCGACAGTGGGGGGTTTAATCTGTTTAACCTGATGAGCGGTTTACTGTGGCTAGGCTTGGTGATGGTGTTCATCCGC
CGCCAACCCGAGGCGGTTGCTACCGAGTAA

Upstream 100 bases:

>100_bases
ACACGCTATTGACGGCAAAATAACCGCTCTGACATGTTCCGACTGTAGGCCCTGCCCAAGCAGTTCCCTTTTACATACTT
ATTATTAATTAGCTTAATTT

Downstream 100 bases:

>100_bases
GTTTATTCCCTGCTGGAGGAGCGTCAGGTCGCTTCTCCAGCAGGATAGTTTTATCCACGCTATATTTCTCAAGACAGTAT
TAAAAACACACCGATACGTA

Product: multidrug translocase MdfA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWRDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSGGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE

Sequences:

>Translated_409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWRDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSGGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE
>Mature_409_residues
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTAYLAGGMFLQWLLGPLSDRRG
RRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLCFIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQTMFVLFAVLGAISFAGLWRAMPETASLKGEKLSVANMWRDYKQVLANRRFLCGSLALGFASLPLLAWIA
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLMIAAGSTLYSSHAYLWMTAGL
SLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMIFTLGIELAKVAYLWGDSGGFNLFNLMSGLLWLGLVMVFIR
RQPEAVATE

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=409, Percent_Identity=71.8826405867971, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790794, Length=377, Percent_Identity=43.236074270557, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI48994889, Length=264, Percent_Identity=28.030303030303, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790146, Length=228, Percent_Identity=28.0701754385965, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788509, Length=258, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082312, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43966; Mature: 43966

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCHHHH
YLAGGMFLQWLLGPLSDRRGRRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLAGAALIHVAPWQTMFVLFAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GAISFAGLWRAMPETASLKGEKLSVANMWRDYKQVLANRRFLCGSLALGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLM
CCCEEEEECCCCCHHHCCCEEHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCHHHHHHH
IAAGSTLYSSHAYLWMTAGLSLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMI
HHCCCHHHHCCCEEEECCCHHHHHHHHHHHCCCHHEEEHHHHHCCCHHHHHHHHHHHHHH
FTLGIELAKVAYLWGDSGGFNLFNLMSGLLWLGLVMVFIRRQPEAVATE
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTSFSPATRLGRRALLFPLCLVLFEFAAYIANDMIQPGMLAVVAEFNASVEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCHHHH
YLAGGMFLQWLLGPLSDRRGRRPVMLAGVAFFVVTCLAILLVNSIEQFIAMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEEAVCIKITALMANVALIAPLLGPLAGAALIHVAPWQTMFVLFAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GAISFAGLWRAMPETASLKGEKLSVANMWRDYKQVLANRRFLCGSLALGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QSPVILISGEQLSTFEYGILQVPIFGALIIGNLTLARLSGKTSIPQLIRYGAGPMIVGLM
CCCEEEEECCCCCHHHCCCEEHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCHHHHHHH
IAAGSTLYSSHAYLWMTAGLSLYAFGIGLANAGLVRLTLFASDISKGTVSAAMGMISMMI
HHCCCHHHHCCCEEEECCCHHHHHHHHHHHCCCHHEEEHHHHHCCCHHHHHHHHHHHHHH
FTLGIELAKVAYLWGDSGGFNLFNLMSGLLWLGLVMVFIRRQPEAVATE
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]