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The map label for this gene is topB [H]

Identifier: 153949900

GI number: 153949900

Start: 2279181

End: 2281106

Strand: Reverse

Name: topB [H]

Synonym: YpsIP31758_1979

Alternate gene names: 153949900

Gene position: 2281106-2279181 (Counterclockwise)

Preceding gene: 153947839

Following gene: 153950795

Centisome position: 48.29

GC content: 49.38

Gene sequence:

>1926_bases
ATGCGACTGTTTATTGCCGAAAAACCCAGTTTAGCGCGAGCGATTGCAGATATTTTGCCAAAACCGCATCGTCGAGGTGA
TGGATTCATCGCCTGTGGTAATGATCAAATGGTGACCTGGTGTGTTGGTCATTTACTCGAACAAGCCCAGCCGGATGCTT
ATGACAGCCGTTATGCACGTTGGTCATTAGCGGACTTACCGATCATTCCTGAAAAATGGCAGCTACAACCTCGTCCTTCC
GTCAGCAAACAACTCAATGTGATTAAGCAACTGTTACAGCAAGCCGATGAAGTGGTCCATGCTGGCGACCCGGATAGAGA
AGGGCAACTGTTAGTTGATGAGGTGCTGGATTATTTGGGATTGGCCGCAGAGAAACGGCAAAATGTTCGTCGTTGCTTGA
TTAACGATTTAAACCCACAGGCGGTTGAGCGTGCAGTGGAGCGCTTGCGTTATAACCGCGAGTTTATCCCGCTGTGTGTT
TCTGCGTTGGCTCGAGCCAGAGCCGATTGGCTCTATGGCATCAATATGACTCGCGCCTATACCATTCTTGGGCGCAATGC
GGGCTATGACGGTGTTTTATCCGTCGGGCGGGTACAAACGCCCGTATTGGGGCTGGTTGTTCGCCGTGATGAAGAGATTG
AAAGCTTCATTCCCAAAGATTTTTTTGAAGTTAAAGCCCACATAGTGACGCCTGCAGACGAACGTTTTATTGCTCTCTGG
CAGCCGAGTGACTCCTGCGAATCTTATCAGGACGAAGAAGGGCGCTTGTTGCATCGGCCGCTTGCAGAACATGTTGTCAA
TCGCATAACCGGCCAGCCCGCGCTGGTGACATCCTATAATGATAAACGGGAATCGGAAACGGCACCGTTGCCATTCTCCT
TGTCTACATTACAAATCGAAGCTGCAAAACGTTTCGGCCTCAGTGCTCAGCAAGTATTGGATATTTGCCAACGGTTGTAT
GAAACGCACAAATTGATTACGTATCCCCGTTCGGATTGCCGATATCTACCTGAAGAGCATTTTGCCGGGCGTCATTCGGT
GCTTAATGCCATTAGTCTTCATCAGTCAGACCTTTTGCCGCTTGAGGTCATGGATAGTGATCGGCGCAACCGCTGCTGGG
ATGATAAAAAAGTCGATGCGCACCACGCTATTATCCCAACCGCACGTGCCAGCAAAGTGAATTTGACGACGGATGAAGGC
AAAGTATACCGGTTGGTGGCACAGCAATATCTGATGCAATTCTGTCCAGATGCACAGTTCCGTAAGTGTGTGATTGAATT
GGATATCGCTGGCGGGAAATTTATTGCCAAGGCACGCTTTTTGGCCGAGGCGGGATGGCGGACCTTACTGGGCAGTAAAG
AGCGTGATGAAGAGAACGAAGGAGCGCCATTGCCGGTGGTAGCCAAAGGGGCTGAATTATTATGCGAACGCGGTGAGGTT
GTTGAACGACAAACTCAGCCACCCCGGCCTTTCACCGACGCCAGTTTATTATCGGCGATGACAGGAATTGCGCGTTTCGT
ACAGGATAAAGAACTGAAAAAAGTTTTGCGAGCCACCGATGGCTTGGGTACGGAAGCCACTCGAGCAGGTATTATCGAAC
TGCTCTTCAAGCGCACTTTTCTCTTTAAGAAAGGGCGTTATATACATGCCAGCCCTGCGGGACGCGCGTTGATTCATGCA
TTACCGGATATTGCGGCACGGCCAGATATGACAGCACATTGGGAATCAACGTTGACACAAATCAGTGAAAAAAACTGCCG
CTATCAAGATTTTATGCAACCACTGGTGATGACATTGCAGGATTTAATTAGTCAGGCGAAACAAAATCGTTCTCCACAGG
TTTTCCGTGGGTTACCCGCCGGCCCTTCAGGGGCACCCAAGAAACGTAAAAAAATATCGTCTAAGGCCAAGGAGAGTAAA
TCATGA

Upstream 100 bases:

>100_bases
GCGGATAGTCGCCGGGCGCTGATTGAGATTGTAGTGTGATGGATTGTAGTGTGATGGATTGTAGCGTGATGTTTTCTTTG
ACGTATTTATGCGGTGAATG

Downstream 100 bases:

>100_bases
AAACACGGTTGCTATTGAGTTGCATTGGCTTTGCCACACTTATTTCTGGTGCGGCATTAGCGAATCGTAGCAATGTTGAT
GTGATTGTTCCTTTGCCACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCV
SALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIALW
QPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEG
KVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIHA
LPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESK
S

Sequences:

>Translated_641_residues
MRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCV
SALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIALW
QPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEG
KVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIHA
LPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESK
S
>Mature_641_residues
MRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCV
SALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIALW
QPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEG
KVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIHA
LPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESK
S

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=624, Percent_Identity=22.9166666666667, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788061, Length=641, Percent_Identity=82.9953198127925, Blast_Score=1117, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=646, Percent_Identity=23.0650154798762, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=618, Percent_Identity=23.462783171521, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=628, Percent_Identity=24.5222929936306, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=672, Percent_Identity=24.2559523809524, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640096, Length=627, Percent_Identity=22.6475279106858, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24585251, Length=698, Percent_Identity=23.6389684813754, Blast_Score=97, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72322; Mature: 72322

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYAR
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHCE
WSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLG
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
LAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRAY
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHEE
TILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIALW
EEEECCCCCCHHHHCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEEE
QPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIE
CCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEEH
AAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLP
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCCC
LEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQF
HHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHH
RKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGEV
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCHH
VERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQ
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYAR
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHCE
WSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLG
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
LAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRAY
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHEE
TILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIALW
EEEECCCCCCHHHHCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEEE
QPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIE
CCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEEH
AAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLP
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCCC
LEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQF
HHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHH
RKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGEV
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCHH
VERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQ
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]