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The map label for this gene is dmlR [H]

Identifier: 153949659

GI number: 153949659

Start: 1753205

End: 1754146

Strand: Reverse

Name: dmlR [H]

Synonym: YpsIP31758_1511

Alternate gene names: 153949659

Gene position: 1754146-1753205 (Counterclockwise)

Preceding gene: 153947162

Following gene: 153948085

Centisome position: 37.14

GC content: 52.76

Gene sequence:

>942_bases
ATGAATAATCTGCCGATACTCAGTGACCTGCGCGTTTTTCTTCTGGTAGCCCGACGCGCAGGTTTTGCCGCTGCCGCTGA
AGAGATGGGGGTTTCTCCCGCCTACGTCAGTAAGCGTGTCGCGATACTGGAAAAAACGCTGAACGTGGCATTACTGCATC
GCACGACACGGAGAGTCACGATTAGTGAGGATGGCGAGCGGACTTATGAATGGGCACAGCGCATTTTGCACGATGTCGAT
CAGATGATGGACGAGCTGTCAGCCATTCGTCAGGAGCCTCAGGGCACCTTAAGGGTTATCAGCAGTTTTGGTTTTGGCCG
ACGCTTTGTGGCCCCCGCACTCTCTGCGTTAGCTCGCGAGTATCCCCGCCTGGAACTGCGGTTTGATGTGGAGGATCGGT
TAATCGATCTGGTGACTGAAGGGGTTGATTTAGATATCCGCGTAGGCGATAACATTGGCGACAACCTGATTGCTTGCCAA
CTCGCCCCGAACCGCCGCATTCTGTGCGCATCCCCCGATTTTCTACAGCGCTACGGCACACCCAAAGCCCTCAGTGAACT
CCCCGGTTTTGCCTGTTTAGTGATCAAAGAGCGTGACCATCCCTTTGGCATATGGCGCTTGCATAATGGCACCGAAGAAC
AATCGATAAAGGTCACTGGCCCGATGGCCTCTAACCATGGCGAAATCGTTCACCAATGGTGCCTGGACGGGCAAGGTATT
GCTTTACGCTCTTACTGGGATGTTAAAGACAATATTCACTCGGGCAAACTGGTGCATATTCTACCGGAGTACTTTCAATC
GGCTAATATCTGGGCGGTGTACGTCACCCGGCTGGCGATGTCGGCAAAAGTCAGAGTGAGCGTAGAATTTTTACGTCGCT
ATTTTAATGAGCACTACGGCGCAGAAAATACATCGGCAAATGGCGGAACGCTGAAGCGCTGA

Upstream 100 bases:

>100_bases
CAAGTCTCATTTATCAGCGGTAAGAATTGACATCTCCTCCATCCTATAATTGACTAATTTACATACAATACAAAAACTGG
TGATACATAAAACACAGATC

Downstream 100 bases:

>100_bases
TAAACCGCCCGCTTATTGTTCCCAACCCACCGCGTAATCGCCCTAAATAAAGCAAAGTCGGGATATTCAGAGAGAAAGAA
TTAACGTGGAATAAAAACTC

Product: LysR family substrate binding transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKTLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR

Sequences:

>Translated_313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKTLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR
>Mature_313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKTLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=74.5819397993311, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=49.3333333333333, Blast_Score=307, Evalue=6e-85,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=30.6122448979592, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=27.681660899654, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.3515358361775, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787589, Length=260, Percent_Identity=26.9230769230769, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.3972602739726, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788706, Length=262, Percent_Identity=28.6259541984733, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=28.6821705426357, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789173, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35346; Mature: 35346

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKTLNVALLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCEEEE
ISEDGERTYEWAQRILHDVDQMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALARE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
YPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQLAPNRRILCASPDFLQRYGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCHHHHHCCC
PKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
HHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYG
EEEEEECCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AENTSANGGTLKR
CCCCCCCCCCCCC
>Mature Secondary Structure
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKTLNVALLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCEEEE
ISEDGERTYEWAQRILHDVDQMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALARE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
YPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQLAPNRRILCASPDFLQRYGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCHHHHHCCC
PKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
HHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYG
EEEEEECCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AENTSANGGTLKR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]